Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20445 and EX31_RS20450 are separated by 104 nucleotides EX31_RS20450 and EX31_RS20455 are separated by 168 nucleotides
EX31_RS20445: EX31_RS20445 - glutamine-hydrolyzing GMP synthase, at 721,957 to 723,534
_RS20445
EX31_RS20450: EX31_RS20450 - IMP dehydrogenase, at 723,639 to 725,105
_RS20450
EX31_RS20455: EX31_RS20455 - exodeoxyribonuclease VII large subunit, at 725,274 to 726,647
_RS20455
Position (kb) 723
724
725
726 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 723.569 kb on - strand at 723.600 kb on - strand at 723.635 kb on - strand at 723.656 kb on - strand at 723.656 kb on - strand at 723.656 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.762 kb on - strand at 723.762 kb on - strand at 723.762 kb on - strand at 723.837 kb on - strand, within EX31_RS20450 at 723.837 kb on - strand, within EX31_RS20450 at 723.839 kb on - strand, within EX31_RS20450 at 723.839 kb on - strand, within EX31_RS20450 at 723.857 kb on + strand, within EX31_RS20450 at 723.902 kb on - strand, within EX31_RS20450 at 723.902 kb on - strand, within EX31_RS20450 at 723.977 kb on - strand, within EX31_RS20450 at 724.055 kb on + strand, within EX31_RS20450 at 724.055 kb on + strand, within EX31_RS20450 at 724.056 kb on - strand, within EX31_RS20450 at 724.188 kb on - strand, within EX31_RS20450 at 724.188 kb on - strand, within EX31_RS20450 at 724.190 kb on + strand, within EX31_RS20450 at 724.190 kb on + strand, within EX31_RS20450 at 724.191 kb on - strand, within EX31_RS20450 at 724.303 kb on + strand, within EX31_RS20450 at 724.304 kb on - strand, within EX31_RS20450 at 724.308 kb on - strand, within EX31_RS20450 at 724.308 kb on - strand, within EX31_RS20450 at 724.308 kb on - strand, within EX31_RS20450 at 724.407 kb on - strand, within EX31_RS20450 at 724.407 kb on - strand, within EX31_RS20450 at 724.473 kb on - strand, within EX31_RS20450 at 724.488 kb on - strand, within EX31_RS20450 at 724.513 kb on - strand, within EX31_RS20450 at 724.513 kb on - strand, within EX31_RS20450 at 724.523 kb on + strand, within EX31_RS20450 at 724.567 kb on - strand, within EX31_RS20450 at 724.704 kb on - strand, within EX31_RS20450 at 724.706 kb on + strand, within EX31_RS20450 at 724.706 kb on + strand, within EX31_RS20450 at 724.707 kb on - strand, within EX31_RS20450 at 724.707 kb on - strand, within EX31_RS20450 at 724.710 kb on - strand, within EX31_RS20450 at 724.814 kb on + strand, within EX31_RS20450 at 724.881 kb on - strand, within EX31_RS20450 at 724.976 kb on + strand at 724.977 kb on - strand at 724.977 kb on - strand at 724.977 kb on - strand at 725.027 kb on + strand at 725.079 kb on + strand at 725.101 kb on - strand at 725.101 kb on - strand at 725.101 kb on - strand at 725.101 kb on - strand at 725.101 kb on - strand at 725.101 kb on - strand at 725.101 kb on - strand at 725.112 kb on + strand at 725.141 kb on - strand at 725.146 kb on - strand at 725.159 kb on + strand at 725.161 kb on + strand at 725.172 kb on - strand at 725.190 kb on + strand at 725.210 kb on + strand at 725.210 kb on + strand at 725.227 kb on + strand at 725.227 kb on + strand at 725.227 kb on + strand at 725.227 kb on + strand at 725.228 kb on - strand at 725.228 kb on - strand at 725.228 kb on - strand at 725.230 kb on + strand at 725.230 kb on + strand at 725.230 kb on + strand at 725.230 kb on + strand at 725.231 kb on - strand at 725.231 kb on - strand at 725.231 kb on - strand at 725.231 kb on - strand at 725.241 kb on + strand at 725.241 kb on + strand at 725.281 kb on + strand at 725.281 kb on + strand at 725.297 kb on + strand at 725.298 kb on - strand at 725.308 kb on + strand at 725.308 kb on + strand at 725.309 kb on - strand at 725.415 kb on + strand, within EX31_RS20455 at 725.415 kb on + strand, within EX31_RS20455 at 725.420 kb on + strand, within EX31_RS20455 at 725.421 kb on - strand, within EX31_RS20455 at 725.512 kb on + strand, within EX31_RS20455 at 725.512 kb on + strand, within EX31_RS20455 at 725.512 kb on + strand, within EX31_RS20455 at 725.513 kb on - strand, within EX31_RS20455 at 725.531 kb on + strand, within EX31_RS20455 at 725.531 kb on + strand, within EX31_RS20455 at 725.531 kb on + strand, within EX31_RS20455 at 725.532 kb on - strand, within EX31_RS20455 at 725.532 kb on - strand, within EX31_RS20455 at 725.532 kb on - strand, within EX31_RS20455 at 725.538 kb on + strand, within EX31_RS20455 at 725.538 kb on + strand, within EX31_RS20455 at 725.538 kb on + strand, within EX31_RS20455 at 725.538 kb on + strand, within EX31_RS20455 at 725.538 kb on + strand, within EX31_RS20455 at 725.538 kb on + strand, within EX31_RS20455 at 725.539 kb on - strand, within EX31_RS20455 at 725.576 kb on + strand, within EX31_RS20455 at 725.576 kb on + strand, within EX31_RS20455 at 725.576 kb on + strand, within EX31_RS20455 at 725.577 kb on - strand, within EX31_RS20455 at 725.577 kb on - strand, within EX31_RS20455 at 725.577 kb on - strand, within EX31_RS20455 at 725.599 kb on + strand, within EX31_RS20455 at 725.600 kb on - strand, within EX31_RS20455 at 725.666 kb on + strand, within EX31_RS20455 at 725.708 kb on + strand, within EX31_RS20455 at 725.708 kb on + strand, within EX31_RS20455 at 725.708 kb on + strand, within EX31_RS20455 at 725.708 kb on + strand, within EX31_RS20455 at 725.709 kb on - strand, within EX31_RS20455 at 725.709 kb on - strand, within EX31_RS20455 at 725.709 kb on - strand, within EX31_RS20455 at 725.709 kb on - strand, within EX31_RS20455 at 725.744 kb on - strand, within EX31_RS20455 at 725.744 kb on - strand, within EX31_RS20455 at 725.753 kb on - strand, within EX31_RS20455 at 725.783 kb on + strand, within EX31_RS20455 at 725.783 kb on + strand, within EX31_RS20455 at 725.783 kb on + strand, within EX31_RS20455 at 725.783 kb on + strand, within EX31_RS20455 at 725.783 kb on + strand, within EX31_RS20455 at 725.784 kb on - strand, within EX31_RS20455 at 725.787 kb on + strand, within EX31_RS20455 at 725.787 kb on + strand, within EX31_RS20455 at 725.911 kb on + strand, within EX31_RS20455 at 725.912 kb on - strand, within EX31_RS20455 at 726.025 kb on + strand, within EX31_RS20455 at 726.025 kb on + strand, within EX31_RS20455 at 726.025 kb on + strand, within EX31_RS20455 at 726.025 kb on + strand, within EX31_RS20455 at 726.025 kb on + strand, within EX31_RS20455 at 726.026 kb on - strand, within EX31_RS20455 at 726.026 kb on - strand, within EX31_RS20455 at 726.026 kb on - strand, within EX31_RS20455
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 723,569 - -0.0 723,600 - +0.1 723,635 - -0.1 723,656 - -0.1 723,656 - -0.5 723,656 - -0.8 723,691 - -2.0 723,691 - -1.5 723,691 - -2.6 723,691 - -2.5 723,691 - -3.2 723,691 - -1.8 723,691 - -3.2 723,691 - -1.4 723,691 - -2.7 723,691 - -1.1 723,691 - -3.8 723,691 - -1.5 723,691 - -1.5 723,691 - -1.1 723,691 - -1.7 723,691 - -2.7 723,691 - -0.4 723,762 - -2.2 723,762 - -2.9 723,762 - -2.6 723,837 - EX31_RS20450 0.13 -1.8 723,837 - EX31_RS20450 0.13 +0.0 723,839 - EX31_RS20450 0.14 -2.2 723,839 - EX31_RS20450 0.14 -3.5 723,857 + EX31_RS20450 0.15 -1.5 723,902 - EX31_RS20450 0.18 -1.8 723,902 - EX31_RS20450 0.18 -1.1 723,977 - EX31_RS20450 0.23 -2.2 724,055 + EX31_RS20450 0.28 -0.8 724,055 + EX31_RS20450 0.28 +1.0 724,056 - EX31_RS20450 0.28 -2.5 724,188 - EX31_RS20450 0.37 -2.7 724,188 - EX31_RS20450 0.37 -2.0 724,190 + EX31_RS20450 0.38 -0.5 724,190 + EX31_RS20450 0.38 -3.0 724,191 - EX31_RS20450 0.38 +0.0 724,303 + EX31_RS20450 0.45 -2.4 724,304 - EX31_RS20450 0.45 +0.2 724,308 - EX31_RS20450 0.46 -3.2 724,308 - EX31_RS20450 0.46 -1.7 724,308 - EX31_RS20450 0.46 -2.4 724,407 - EX31_RS20450 0.52 -0.9 724,407 - EX31_RS20450 0.52 -0.8 724,473 - EX31_RS20450 0.57 -1.5 724,488 - EX31_RS20450 0.58 -1.7 724,513 - EX31_RS20450 0.60 -2.4 724,513 - EX31_RS20450 0.60 -1.4 724,523 + EX31_RS20450 0.60 -2.0 724,567 - EX31_RS20450 0.63 -2.9 724,704 - EX31_RS20450 0.73 -0.8 724,706 + EX31_RS20450 0.73 -0.5 724,706 + EX31_RS20450 0.73 -0.5 724,707 - EX31_RS20450 0.73 -2.9 724,707 - EX31_RS20450 0.73 -2.4 724,710 - EX31_RS20450 0.73 -1.3 724,814 + EX31_RS20450 0.80 -3.2 724,881 - EX31_RS20450 0.85 -1.0 724,976 + -1.9 724,977 - -3.1 724,977 - -3.7 724,977 - -2.9 725,027 + -0.8 725,079 + -0.8 725,101 - -1.5 725,101 - -3.0 725,101 - -2.5 725,101 - -2.4 725,101 - +0.0 725,101 - -3.8 725,101 - -3.0 725,112 + -2.0 725,141 - -0.7 725,146 - -0.2 725,159 + +0.0 725,161 + -3.2 725,172 - -0.8 725,190 + +1.0 725,210 + -0.2 725,210 + -0.5 725,227 + -1.8 725,227 + -0.3 725,227 + +0.4 725,227 + -0.9 725,228 - +1.5 725,228 - +1.5 725,228 - +0.0 725,230 + +0.7 725,230 + +1.3 725,230 + -0.4 725,230 + -1.4 725,231 - +0.6 725,231 - +0.4 725,231 - +0.8 725,231 - -0.0 725,241 + +0.6 725,241 + -1.2 725,281 + -0.5 725,281 + +0.6 725,297 + -0.1 725,298 - +1.3 725,308 + -0.6 725,308 + -0.2 725,309 - -1.7 725,415 + EX31_RS20455 0.10 +0.9 725,415 + EX31_RS20455 0.10 +0.1 725,420 + EX31_RS20455 0.11 +1.5 725,421 - EX31_RS20455 0.11 -0.5 725,512 + EX31_RS20455 0.17 -0.3 725,512 + EX31_RS20455 0.17 -1.4 725,512 + EX31_RS20455 0.17 -0.1 725,513 - EX31_RS20455 0.17 -0.7 725,531 + EX31_RS20455 0.19 +1.6 725,531 + EX31_RS20455 0.19 -1.6 725,531 + EX31_RS20455 0.19 +0.3 725,532 - EX31_RS20455 0.19 -0.2 725,532 - EX31_RS20455 0.19 -0.1 725,532 - EX31_RS20455 0.19 +0.4 725,538 + EX31_RS20455 0.19 -0.3 725,538 + EX31_RS20455 0.19 -0.7 725,538 + EX31_RS20455 0.19 +0.5 725,538 + EX31_RS20455 0.19 -0.4 725,538 + EX31_RS20455 0.19 -2.0 725,538 + EX31_RS20455 0.19 +0.5 725,539 - EX31_RS20455 0.19 +0.5 725,576 + EX31_RS20455 0.22 +0.2 725,576 + EX31_RS20455 0.22 +0.0 725,576 + EX31_RS20455 0.22 -0.2 725,577 - EX31_RS20455 0.22 +0.8 725,577 - EX31_RS20455 0.22 -1.0 725,577 - EX31_RS20455 0.22 -0.7 725,599 + EX31_RS20455 0.24 -0.8 725,600 - EX31_RS20455 0.24 -0.0 725,666 + EX31_RS20455 0.29 +0.3 725,708 + EX31_RS20455 0.32 +0.5 725,708 + EX31_RS20455 0.32 +0.1 725,708 + EX31_RS20455 0.32 -0.4 725,708 + EX31_RS20455 0.32 -0.5 725,709 - EX31_RS20455 0.32 -0.5 725,709 - EX31_RS20455 0.32 +0.3 725,709 - EX31_RS20455 0.32 -1.7 725,709 - EX31_RS20455 0.32 +0.0 725,744 - EX31_RS20455 0.34 +0.1 725,744 - EX31_RS20455 0.34 -0.7 725,753 - EX31_RS20455 0.35 +1.0 725,783 + EX31_RS20455 0.37 +2.0 725,783 + EX31_RS20455 0.37 -1.0 725,783 + EX31_RS20455 0.37 +0.6 725,783 + EX31_RS20455 0.37 -0.8 725,783 + EX31_RS20455 0.37 -0.8 725,784 - EX31_RS20455 0.37 +0.7 725,787 + EX31_RS20455 0.37 -0.5 725,787 + EX31_RS20455 0.37 -0.1 725,911 + EX31_RS20455 0.46 -1.6 725,912 - EX31_RS20455 0.46 +0.2 726,025 + EX31_RS20455 0.55 -0.1 726,025 + EX31_RS20455 0.55 -0.4 726,025 + EX31_RS20455 0.55 +1.1 726,025 + EX31_RS20455 0.55 +0.4 726,025 + EX31_RS20455 0.55 -0.0 726,026 - EX31_RS20455 0.55 -0.8 726,026 - EX31_RS20455 0.55 +0.2 726,026 - EX31_RS20455 0.55 +0.9
Or see this region's nucleotide sequence