Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS16320 and EX31_RS16325 are separated by 149 nucleotides EX31_RS16325 and EX31_RS16330 overlap by 1 nucleotides EX31_RS16330 and EX31_RS16335 are separated by 218 nucleotides EX31_RS16335 and EX31_RS16340 are separated by 102 nucleotides
EX31_RS16320: EX31_RS16320 - conjugal transfer protein TraT, at 11,690 to 12,424
_RS16320
EX31_RS16325: EX31_RS16325 - AbrB/MazE/SpoVT family DNA-binding domain-containing protein, at 12,574 to 12,819
_RS16325
EX31_RS16330: EX31_RS16330 - endoribonuclease MazF, at 12,819 to 13,151
_RS16330
EX31_RS16335: EX31_RS16335 - hypothetical protein, at 13,370 to 13,717
_RS16335
EX31_RS16340: EX31_RS16340 - type IV conjugative transfer system coupling protein TraD, at 13,820 to 16,507
_RS16340
Position (kb) 13
14 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 12.370 kb on + strand at 12.370 kb on + strand at 12.422 kb on + strand at 12.422 kb on + strand at 12.422 kb on + strand at 12.422 kb on + strand at 12.422 kb on + strand at 12.441 kb on + strand at 12.441 kb on + strand at 12.441 kb on + strand at 12.471 kb on - strand at 12.471 kb on - strand at 12.471 kb on - strand at 12.471 kb on - strand at 12.501 kb on - strand at 12.504 kb on + strand at 12.528 kb on + strand at 12.528 kb on + strand at 12.529 kb on - strand at 12.531 kb on + strand at 12.532 kb on - strand at 12.538 kb on - strand at 12.542 kb on + strand at 12.585 kb on + strand at 12.585 kb on + strand at 12.719 kb on + strand, within EX31_RS16325 at 12.777 kb on + strand, within EX31_RS16325 at 12.777 kb on + strand, within EX31_RS16325 at 12.777 kb on + strand, within EX31_RS16325 at 12.812 kb on + strand at 12.812 kb on + strand at 12.933 kb on + strand, within EX31_RS16330 at 12.933 kb on + strand, within EX31_RS16330 at 12.933 kb on + strand, within EX31_RS16330 at 12.933 kb on + strand, within EX31_RS16330 at 12.933 kb on + strand, within EX31_RS16330 at 12.933 kb on + strand, within EX31_RS16330 at 12.933 kb on + strand, within EX31_RS16330 at 12.935 kb on + strand, within EX31_RS16330 at 12.955 kb on + strand, within EX31_RS16330 at 12.955 kb on + strand, within EX31_RS16330 at 12.955 kb on + strand, within EX31_RS16330 at 12.955 kb on + strand, within EX31_RS16330 at 13.009 kb on + strand, within EX31_RS16330 at 13.120 kb on + strand at 13.120 kb on + strand at 13.133 kb on + strand at 13.133 kb on + strand at 13.133 kb on + strand at 13.144 kb on + strand at 13.144 kb on + strand at 13.144 kb on + strand at 13.144 kb on + strand at 13.144 kb on + strand at 13.145 kb on - strand at 13.170 kb on - strand at 13.170 kb on - strand at 13.172 kb on + strand at 13.187 kb on + strand at 13.190 kb on + strand at 13.190 kb on + strand at 13.190 kb on + strand at 13.191 kb on - strand at 13.218 kb on + strand at 13.218 kb on + strand at 13.219 kb on - strand at 13.223 kb on + strand at 13.223 kb on + strand at 13.223 kb on + strand at 13.223 kb on + strand at 13.223 kb on + strand at 13.223 kb on + strand at 13.223 kb on + strand at 13.223 kb on + strand at 13.223 kb on + strand at 13.223 kb on + strand at 13.223 kb on + strand at 13.224 kb on - strand at 13.224 kb on - strand at 13.224 kb on - strand at 13.224 kb on - strand at 13.224 kb on - strand at 13.224 kb on - strand at 13.224 kb on - strand at 13.224 kb on - strand at 13.233 kb on - strand at 13.233 kb on - strand at 13.233 kb on - strand at 13.233 kb on - strand at 13.233 kb on - strand at 13.246 kb on + strand at 13.246 kb on + strand at 13.247 kb on - strand at 13.247 kb on - strand at 13.374 kb on + strand at 13.374 kb on + strand at 13.374 kb on + strand at 13.374 kb on + strand at 13.375 kb on - strand at 13.375 kb on - strand at 13.375 kb on - strand at 13.375 kb on - strand at 13.375 kb on - strand at 13.375 kb on - strand at 13.376 kb on + strand at 13.376 kb on + strand at 13.377 kb on - strand at 13.377 kb on - strand at 13.377 kb on - strand at 13.377 kb on - strand at 13.408 kb on + strand, within EX31_RS16335 at 13.486 kb on + strand, within EX31_RS16335 at 13.487 kb on - strand, within EX31_RS16335 at 13.487 kb on - strand, within EX31_RS16335 at 13.487 kb on - strand, within EX31_RS16335 at 13.532 kb on - strand, within EX31_RS16335 at 13.557 kb on + strand, within EX31_RS16335 at 13.558 kb on - strand, within EX31_RS16335 at 13.566 kb on + strand, within EX31_RS16335 at 13.620 kb on + strand, within EX31_RS16335 at 13.620 kb on + strand, within EX31_RS16335 at 13.620 kb on + strand, within EX31_RS16335 at 13.681 kb on + strand, within EX31_RS16335 at 13.682 kb on - strand, within EX31_RS16335 at 13.682 kb on - strand, within EX31_RS16335 at 13.699 kb on + strand at 13.699 kb on + strand at 13.699 kb on + strand at 13.699 kb on + strand at 13.699 kb on + strand at 13.700 kb on - strand at 13.700 kb on - strand at 13.700 kb on - strand at 13.700 kb on - strand at 13.700 kb on - strand at 13.700 kb on - strand at 13.700 kb on - strand at 13.700 kb on - strand at 13.700 kb on - strand at 13.700 kb on - strand at 13.700 kb on - strand at 13.716 kb on - strand at 13.724 kb on + strand at 13.791 kb on + strand at 13.791 kb on + strand at 13.828 kb on + strand at 13.838 kb on + strand at 13.838 kb on + strand at 13.838 kb on + strand at 13.838 kb on + strand at 13.839 kb on - strand at 13.839 kb on - strand at 13.932 kb on + strand at 13.935 kb on + strand at 13.936 kb on - strand at 13.950 kb on + strand at 13.951 kb on - strand at 13.951 kb on - strand at 13.951 kb on - strand at 13.963 kb on + strand at 13.963 kb on + strand at 13.963 kb on + strand at 13.963 kb on + strand at 13.963 kb on + strand at 13.964 kb on - strand at 13.964 kb on - strand at 13.985 kb on - strand at 14.091 kb on + strand, within EX31_RS16340 at 14.091 kb on + strand, within EX31_RS16340 at 14.091 kb on + strand, within EX31_RS16340 at 14.092 kb on - strand, within EX31_RS16340 at 14.092 kb on - strand, within EX31_RS16340 at 14.178 kb on + strand, within EX31_RS16340 at 14.178 kb on + strand, within EX31_RS16340 at 14.179 kb on - strand, within EX31_RS16340 at 14.240 kb on + strand, within EX31_RS16340 at 14.241 kb on - strand, within EX31_RS16340 at 14.241 kb on - strand, within EX31_RS16340 at 14.241 kb on - strand, within EX31_RS16340 at 14.241 kb on - strand, within EX31_RS16340 at 14.275 kb on + strand, within EX31_RS16340 at 14.275 kb on + strand, within EX31_RS16340 at 14.275 kb on + strand, within EX31_RS16340 at 14.275 kb on + strand, within EX31_RS16340 at 14.275 kb on + strand, within EX31_RS16340 at 14.275 kb on + strand, within EX31_RS16340 at 14.275 kb on + strand, within EX31_RS16340 at 14.275 kb on + strand, within EX31_RS16340 at 14.276 kb on - strand, within EX31_RS16340 at 14.276 kb on - strand, within EX31_RS16340 at 14.276 kb on - strand, within EX31_RS16340 at 14.276 kb on - strand, within EX31_RS16340 at 14.276 kb on - strand, within EX31_RS16340 at 14.276 kb on - strand, within EX31_RS16340 at 14.276 kb on - strand, within EX31_RS16340 at 14.279 kb on + strand, within EX31_RS16340 at 14.279 kb on + strand, within EX31_RS16340 at 14.279 kb on + strand, within EX31_RS16340 at 14.279 kb on + strand, within EX31_RS16340 at 14.279 kb on + strand, within EX31_RS16340 at 14.279 kb on + strand, within EX31_RS16340 at 14.279 kb on + strand, within EX31_RS16340 at 14.279 kb on + strand, within EX31_RS16340 at 14.279 kb on + strand, within EX31_RS16340 at 14.279 kb on + strand, within EX31_RS16340 at 14.279 kb on + strand, within EX31_RS16340 at 14.279 kb on + strand, within EX31_RS16340 at 14.279 kb on + strand, within EX31_RS16340 at 14.279 kb on + strand, within EX31_RS16340 at 14.280 kb on - strand, within EX31_RS16340 at 14.280 kb on - strand, within EX31_RS16340 at 14.280 kb on - strand, within EX31_RS16340 at 14.280 kb on - strand, within EX31_RS16340 at 14.280 kb on - strand, within EX31_RS16340 at 14.280 kb on - strand, within EX31_RS16340 at 14.280 kb on - strand, within EX31_RS16340 at 14.280 kb on - strand, within EX31_RS16340 at 14.280 kb on - strand, within EX31_RS16340 at 14.280 kb on - strand, within EX31_RS16340 at 14.319 kb on + strand, within EX31_RS16340 at 14.319 kb on + strand, within EX31_RS16340 at 14.373 kb on + strand, within EX31_RS16340 at 14.374 kb on - strand, within EX31_RS16340 at 14.374 kb on - strand, within EX31_RS16340 at 14.374 kb on - strand, within EX31_RS16340 at 14.398 kb on - strand, within EX31_RS16340 at 14.398 kb on - strand, within EX31_RS16340 at 14.398 kb on - strand, within EX31_RS16340 at 14.405 kb on + strand, within EX31_RS16340 at 14.405 kb on + strand, within EX31_RS16340 at 14.405 kb on + strand, within EX31_RS16340 at 14.406 kb on - strand, within EX31_RS16340 at 14.406 kb on - strand, within EX31_RS16340 at 14.406 kb on - strand, within EX31_RS16340 at 14.406 kb on - strand, within EX31_RS16340 at 14.406 kb on - strand, within EX31_RS16340 at 14.412 kb on + strand, within EX31_RS16340 at 14.422 kb on + strand, within EX31_RS16340 at 14.423 kb on - strand, within EX31_RS16340 at 14.423 kb on - strand, within EX31_RS16340 at 14.467 kb on + strand, within EX31_RS16340 at 14.543 kb on - strand, within EX31_RS16340 at 14.543 kb on - strand, within EX31_RS16340 at 14.543 kb on - strand, within EX31_RS16340 at 14.645 kb on - strand, within EX31_RS16340 at 14.645 kb on - strand, within EX31_RS16340
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 12,370 + +0.0 12,370 + -0.1 12,422 + -1.4 12,422 + +0.0 12,422 + -0.1 12,422 + -0.1 12,422 + -0.4 12,441 + -0.2 12,441 + -0.7 12,441 + +0.6 12,471 - -0.8 12,471 - +0.3 12,471 - +0.9 12,471 - -0.3 12,501 - +0.3 12,504 + +0.6 12,528 + +0.3 12,528 + -0.4 12,529 - +1.3 12,531 + +0.7 12,532 - +0.0 12,538 - -0.2 12,542 + -1.4 12,585 + +2.3 12,585 + +0.8 12,719 + EX31_RS16325 0.59 -2.3 12,777 + EX31_RS16325 0.83 +1.6 12,777 + EX31_RS16325 0.83 +0.6 12,777 + EX31_RS16325 0.83 +1.1 12,812 + +0.1 12,812 + +1.5 12,933 + EX31_RS16330 0.34 +0.4 12,933 + EX31_RS16330 0.34 +0.5 12,933 + EX31_RS16330 0.34 -0.2 12,933 + EX31_RS16330 0.34 +0.1 12,933 + EX31_RS16330 0.34 +0.1 12,933 + EX31_RS16330 0.34 -1.0 12,933 + EX31_RS16330 0.34 -0.1 12,935 + EX31_RS16330 0.35 +1.3 12,955 + EX31_RS16330 0.41 +1.3 12,955 + EX31_RS16330 0.41 -0.3 12,955 + EX31_RS16330 0.41 +0.0 12,955 + EX31_RS16330 0.41 +0.1 13,009 + EX31_RS16330 0.57 +1.0 13,120 + -1.7 13,120 + +0.3 13,133 + +0.0 13,133 + +0.8 13,133 + -0.1 13,144 + +0.4 13,144 + -1.0 13,144 + +0.9 13,144 + +0.9 13,144 + +1.3 13,145 - +1.3 13,170 - +0.7 13,170 - +0.4 13,172 + +0.3 13,187 + +0.3 13,190 + +0.4 13,190 + +0.1 13,190 + +0.6 13,191 - +0.0 13,218 + +0.6 13,218 + -1.3 13,219 - +0.1 13,223 + -1.1 13,223 + +2.0 13,223 + +0.8 13,223 + -0.5 13,223 + -0.5 13,223 + -0.1 13,223 + -1.1 13,223 + +0.3 13,223 + -0.5 13,223 + +0.5 13,223 + -0.5 13,224 - +0.4 13,224 - +1.3 13,224 - +0.3 13,224 - -0.2 13,224 - +0.8 13,224 - +0.5 13,224 - +0.4 13,224 - +1.1 13,233 - +1.0 13,233 - +0.5 13,233 - +1.9 13,233 - +0.7 13,233 - -0.4 13,246 + +0.6 13,246 + +0.1 13,247 - +0.8 13,247 - -0.4 13,374 + -1.3 13,374 + +0.7 13,374 + +0.5 13,374 + +1.1 13,375 - -0.4 13,375 - -1.0 13,375 - -1.8 13,375 - +1.3 13,375 - +0.8 13,375 - -0.6 13,376 + +0.5 13,376 + -0.9 13,377 - -0.2 13,377 - +1.3 13,377 - +0.0 13,377 - -0.6 13,408 + EX31_RS16335 0.11 +1.0 13,486 + EX31_RS16335 0.33 -0.1 13,487 - EX31_RS16335 0.34 -0.0 13,487 - EX31_RS16335 0.34 +1.3 13,487 - EX31_RS16335 0.34 -0.9 13,532 - EX31_RS16335 0.47 -0.8 13,557 + EX31_RS16335 0.54 +0.6 13,558 - EX31_RS16335 0.54 -0.8 13,566 + EX31_RS16335 0.56 -1.4 13,620 + EX31_RS16335 0.72 +0.9 13,620 + EX31_RS16335 0.72 +0.7 13,620 + EX31_RS16335 0.72 +3.3 13,681 + EX31_RS16335 0.89 +0.8 13,682 - EX31_RS16335 0.90 +1.5 13,682 - EX31_RS16335 0.90 +1.3 13,699 + +0.1 13,699 + -0.2 13,699 + -1.1 13,699 + -0.8 13,699 + +0.4 13,700 - +0.8 13,700 - +0.8 13,700 - -0.1 13,700 - +0.3 13,700 - +0.3 13,700 - +0.3 13,700 - -0.3 13,700 - -0.1 13,700 - -0.7 13,700 - +0.4 13,700 - +1.3 13,716 - +0.1 13,724 + +1.8 13,791 + +1.8 13,791 + +2.3 13,828 + +0.8 13,838 + +1.3 13,838 + -0.2 13,838 + -0.2 13,838 + -0.3 13,839 - +1.0 13,839 - +1.0 13,932 + +0.3 13,935 + +0.1 13,936 - +1.0 13,950 + +0.4 13,951 - +0.9 13,951 - -0.6 13,951 - +1.3 13,963 + -0.7 13,963 + +0.2 13,963 + -0.8 13,963 + +0.4 13,963 + +0.7 13,964 - -0.1 13,964 - -0.2 13,985 - -0.6 14,091 + EX31_RS16340 0.10 -0.4 14,091 + EX31_RS16340 0.10 -1.6 14,091 + EX31_RS16340 0.10 +0.3 14,092 - EX31_RS16340 0.10 +0.7 14,092 - EX31_RS16340 0.10 -0.5 14,178 + EX31_RS16340 0.13 -0.3 14,178 + EX31_RS16340 0.13 -1.4 14,179 - EX31_RS16340 0.13 -0.4 14,240 + EX31_RS16340 0.16 +1.1 14,241 - EX31_RS16340 0.16 +1.0 14,241 - EX31_RS16340 0.16 +1.1 14,241 - EX31_RS16340 0.16 +1.1 14,241 - EX31_RS16340 0.16 -1.7 14,275 + EX31_RS16340 0.17 +0.8 14,275 + EX31_RS16340 0.17 +0.2 14,275 + EX31_RS16340 0.17 +0.6 14,275 + EX31_RS16340 0.17 -2.0 14,275 + EX31_RS16340 0.17 +1.7 14,275 + EX31_RS16340 0.17 +0.8 14,275 + EX31_RS16340 0.17 +0.0 14,275 + EX31_RS16340 0.17 -0.2 14,276 - EX31_RS16340 0.17 -0.0 14,276 - EX31_RS16340 0.17 +0.0 14,276 - EX31_RS16340 0.17 -0.5 14,276 - EX31_RS16340 0.17 +0.4 14,276 - EX31_RS16340 0.17 -0.3 14,276 - EX31_RS16340 0.17 +0.5 14,276 - EX31_RS16340 0.17 +0.4 14,279 + EX31_RS16340 0.17 +0.5 14,279 + EX31_RS16340 0.17 +0.5 14,279 + EX31_RS16340 0.17 +1.6 14,279 + EX31_RS16340 0.17 +1.8 14,279 + EX31_RS16340 0.17 +0.1 14,279 + EX31_RS16340 0.17 +0.8 14,279 + EX31_RS16340 0.17 -1.1 14,279 + EX31_RS16340 0.17 +1.3 14,279 + EX31_RS16340 0.17 +0.4 14,279 + EX31_RS16340 0.17 -0.3 14,279 + EX31_RS16340 0.17 +0.9 14,279 + EX31_RS16340 0.17 +0.4 14,279 + EX31_RS16340 0.17 -0.3 14,279 + EX31_RS16340 0.17 +1.8 14,280 - EX31_RS16340 0.17 +0.7 14,280 - EX31_RS16340 0.17 +1.7 14,280 - EX31_RS16340 0.17 -0.2 14,280 - EX31_RS16340 0.17 +0.6 14,280 - EX31_RS16340 0.17 -0.5 14,280 - EX31_RS16340 0.17 +0.0 14,280 - EX31_RS16340 0.17 -0.8 14,280 - EX31_RS16340 0.17 +1.3 14,280 - EX31_RS16340 0.17 +1.7 14,280 - EX31_RS16340 0.17 +1.4 14,319 + EX31_RS16340 0.19 +1.0 14,319 + EX31_RS16340 0.19 -2.0 14,373 + EX31_RS16340 0.21 +0.5 14,374 - EX31_RS16340 0.21 +0.3 14,374 - EX31_RS16340 0.21 -1.5 14,374 - EX31_RS16340 0.21 +1.4 14,398 - EX31_RS16340 0.22 -1.2 14,398 - EX31_RS16340 0.22 -1.8 14,398 - EX31_RS16340 0.22 -0.5 14,405 + EX31_RS16340 0.22 -0.6 14,405 + EX31_RS16340 0.22 +0.6 14,405 + EX31_RS16340 0.22 -1.3 14,406 - EX31_RS16340 0.22 +0.5 14,406 - EX31_RS16340 0.22 -1.3 14,406 - EX31_RS16340 0.22 -0.2 14,406 - EX31_RS16340 0.22 -0.1 14,406 - EX31_RS16340 0.22 -1.7 14,412 + EX31_RS16340 0.22 -0.6 14,422 + EX31_RS16340 0.22 +0.7 14,423 - EX31_RS16340 0.22 -0.2 14,423 - EX31_RS16340 0.22 -0.6 14,467 + EX31_RS16340 0.24 -0.9 14,543 - EX31_RS16340 0.27 +1.3 14,543 - EX31_RS16340 0.27 +0.6 14,543 - EX31_RS16340 0.27 -1.1 14,645 - EX31_RS16340 0.31 +0.4 14,645 - EX31_RS16340 0.31 -0.3
Or see this region's nucleotide sequence