Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS14295 and EX31_RS14300 are separated by 127 nucleotides EX31_RS14300 and EX31_RS14305 are separated by 67 nucleotides EX31_RS14305 and EX31_RS14310 are separated by 184 nucleotides
EX31_RS14295: EX31_RS14295 - DUF1454 family protein, at 41,146 to 41,754
_RS14295
EX31_RS14300: EX31_RS14300 - triose-phosphate isomerase, at 41,882 to 42,649
_RS14300
EX31_RS14305: EX31_RS14305 - sulfate ABC transporter substrate-binding protein, at 42,717 to 43,706
_RS14305
EX31_RS14310: EX31_RS14310 - 6-phosphofructokinase, at 43,891 to 44,877
_RS14310
Position (kb) 42
43
44 Strain fitness (log2 ratio) -2
-1
0
1 at 41.767 kb on + strand at 41.789 kb on + strand at 41.805 kb on + strand at 41.806 kb on - strand at 41.806 kb on - strand at 41.807 kb on + strand at 41.807 kb on + strand at 41.807 kb on + strand at 41.807 kb on + strand at 41.807 kb on + strand at 41.807 kb on + strand at 41.808 kb on - strand at 41.821 kb on + strand at 41.821 kb on + strand at 41.821 kb on + strand at 41.821 kb on + strand at 41.822 kb on - strand at 41.830 kb on + strand at 41.830 kb on + strand at 41.830 kb on + strand at 41.830 kb on + strand at 42.638 kb on - strand at 42.638 kb on - strand at 42.647 kb on + strand at 42.647 kb on + strand at 42.707 kb on - strand at 42.707 kb on - strand at 42.874 kb on + strand, within EX31_RS14305 at 42.910 kb on + strand, within EX31_RS14305 at 42.911 kb on - strand, within EX31_RS14305 at 42.916 kb on + strand, within EX31_RS14305 at 42.916 kb on + strand, within EX31_RS14305 at 42.916 kb on + strand, within EX31_RS14305 at 42.916 kb on + strand, within EX31_RS14305 at 42.916 kb on + strand, within EX31_RS14305 at 42.916 kb on + strand, within EX31_RS14305 at 42.916 kb on + strand, within EX31_RS14305 at 42.916 kb on + strand, within EX31_RS14305 at 42.917 kb on - strand, within EX31_RS14305 at 42.917 kb on - strand, within EX31_RS14305 at 42.917 kb on - strand, within EX31_RS14305 at 42.917 kb on - strand, within EX31_RS14305 at 42.917 kb on - strand, within EX31_RS14305 at 42.917 kb on - strand, within EX31_RS14305 at 42.926 kb on - strand, within EX31_RS14305 at 42.941 kb on + strand, within EX31_RS14305 at 42.941 kb on + strand, within EX31_RS14305 at 42.942 kb on - strand, within EX31_RS14305 at 42.956 kb on + strand, within EX31_RS14305 at 42.956 kb on + strand, within EX31_RS14305 at 42.968 kb on + strand, within EX31_RS14305 at 43.031 kb on + strand, within EX31_RS14305 at 43.031 kb on + strand, within EX31_RS14305 at 43.032 kb on - strand, within EX31_RS14305 at 43.115 kb on + strand, within EX31_RS14305 at 43.115 kb on + strand, within EX31_RS14305 at 43.115 kb on + strand, within EX31_RS14305 at 43.116 kb on - strand, within EX31_RS14305 at 43.118 kb on + strand, within EX31_RS14305 at 43.119 kb on - strand, within EX31_RS14305 at 43.119 kb on - strand, within EX31_RS14305 at 43.160 kb on + strand, within EX31_RS14305 at 43.161 kb on - strand, within EX31_RS14305 at 43.163 kb on - strand, within EX31_RS14305 at 43.163 kb on - strand, within EX31_RS14305 at 43.163 kb on - strand, within EX31_RS14305 at 43.163 kb on - strand, within EX31_RS14305 at 43.240 kb on + strand, within EX31_RS14305 at 43.241 kb on - strand, within EX31_RS14305 at 43.241 kb on - strand, within EX31_RS14305 at 43.268 kb on + strand, within EX31_RS14305 at 43.268 kb on + strand, within EX31_RS14305 at 43.274 kb on + strand, within EX31_RS14305 at 43.274 kb on + strand, within EX31_RS14305 at 43.280 kb on + strand, within EX31_RS14305 at 43.280 kb on + strand, within EX31_RS14305 at 43.281 kb on - strand, within EX31_RS14305 at 43.281 kb on - strand, within EX31_RS14305 at 43.281 kb on - strand, within EX31_RS14305 at 43.290 kb on + strand, within EX31_RS14305 at 43.372 kb on + strand, within EX31_RS14305 at 43.372 kb on + strand, within EX31_RS14305 at 43.373 kb on - strand, within EX31_RS14305 at 43.436 kb on + strand, within EX31_RS14305 at 43.436 kb on + strand, within EX31_RS14305 at 43.437 kb on - strand, within EX31_RS14305 at 43.450 kb on + strand, within EX31_RS14305 at 43.450 kb on + strand, within EX31_RS14305 at 43.450 kb on + strand, within EX31_RS14305 at 43.451 kb on - strand, within EX31_RS14305 at 43.451 kb on - strand, within EX31_RS14305 at 43.468 kb on - strand, within EX31_RS14305 at 43.490 kb on + strand, within EX31_RS14305 at 43.490 kb on + strand, within EX31_RS14305 at 43.490 kb on + strand, within EX31_RS14305 at 43.494 kb on + strand, within EX31_RS14305 at 43.495 kb on - strand, within EX31_RS14305 at 43.495 kb on - strand, within EX31_RS14305 at 43.495 kb on - strand, within EX31_RS14305 at 43.495 kb on - strand, within EX31_RS14305 at 43.502 kb on + strand, within EX31_RS14305 at 43.502 kb on + strand, within EX31_RS14305 at 43.502 kb on + strand, within EX31_RS14305 at 43.502 kb on + strand, within EX31_RS14305 at 43.502 kb on + strand, within EX31_RS14305 at 43.503 kb on - strand, within EX31_RS14305 at 43.503 kb on - strand, within EX31_RS14305 at 43.537 kb on - strand, within EX31_RS14305 at 43.547 kb on + strand, within EX31_RS14305 at 43.570 kb on + strand, within EX31_RS14305 at 43.571 kb on - strand, within EX31_RS14305 at 43.571 kb on - strand, within EX31_RS14305 at 43.574 kb on + strand, within EX31_RS14305 at 43.575 kb on - strand, within EX31_RS14305 at 43.589 kb on + strand, within EX31_RS14305 at 43.589 kb on + strand, within EX31_RS14305 at 43.589 kb on + strand, within EX31_RS14305 at 43.589 kb on + strand, within EX31_RS14305 at 43.589 kb on + strand, within EX31_RS14305 at 43.589 kb on + strand, within EX31_RS14305 at 43.589 kb on + strand, within EX31_RS14305 at 43.589 kb on + strand, within EX31_RS14305 at 43.589 kb on + strand, within EX31_RS14305 at 43.590 kb on - strand, within EX31_RS14305 at 43.590 kb on - strand, within EX31_RS14305 at 43.590 kb on - strand, within EX31_RS14305 at 43.590 kb on - strand, within EX31_RS14305 at 43.590 kb on - strand, within EX31_RS14305 at 43.590 kb on - strand, within EX31_RS14305 at 43.590 kb on - strand, within EX31_RS14305 at 43.590 kb on - strand, within EX31_RS14305 at 43.590 kb on - strand, within EX31_RS14305 at 43.590 kb on - strand, within EX31_RS14305 at 43.591 kb on + strand, within EX31_RS14305 at 43.591 kb on + strand, within EX31_RS14305 at 43.591 kb on + strand, within EX31_RS14305 at 43.591 kb on + strand, within EX31_RS14305 at 43.591 kb on + strand, within EX31_RS14305 at 43.591 kb on + strand, within EX31_RS14305 at 43.591 kb on + strand, within EX31_RS14305 at 43.592 kb on - strand, within EX31_RS14305 at 43.592 kb on - strand, within EX31_RS14305 at 43.592 kb on - strand, within EX31_RS14305 at 43.592 kb on - strand, within EX31_RS14305 at 43.592 kb on - strand, within EX31_RS14305 at 43.592 kb on - strand, within EX31_RS14305 at 43.592 kb on - strand, within EX31_RS14305 at 43.592 kb on - strand, within EX31_RS14305 at 43.592 kb on - strand, within EX31_RS14305 at 43.592 kb on - strand, within EX31_RS14305 at 43.592 kb on - strand, within EX31_RS14305 at 43.592 kb on - strand, within EX31_RS14305 at 43.592 kb on - strand, within EX31_RS14305 at 43.592 kb on - strand, within EX31_RS14305 at 43.600 kb on + strand, within EX31_RS14305 at 43.600 kb on + strand, within EX31_RS14305 at 43.600 kb on + strand, within EX31_RS14305 at 43.601 kb on - strand, within EX31_RS14305 at 43.622 kb on - strand at 43.622 kb on - strand at 43.646 kb on - strand at 43.700 kb on + strand at 43.700 kb on + strand at 43.700 kb on + strand at 43.701 kb on - strand at 43.701 kb on - strand at 43.701 kb on - strand at 43.701 kb on - strand at 43.701 kb on - strand at 43.709 kb on + strand at 43.709 kb on + strand at 43.709 kb on + strand at 43.710 kb on - strand at 43.710 kb on - strand at 43.710 kb on - strand at 43.710 kb on - strand at 43.722 kb on + strand at 43.722 kb on + strand at 43.722 kb on + strand at 43.722 kb on + strand at 43.723 kb on - strand at 43.723 kb on - strand at 43.723 kb on - strand at 43.723 kb on - strand at 43.723 kb on - strand at 43.723 kb on - strand at 43.729 kb on + strand at 43.742 kb on + strand at 43.743 kb on - strand at 43.743 kb on - strand at 43.771 kb on + strand at 43.787 kb on - strand at 43.794 kb on - strand at 43.812 kb on - strand at 43.883 kb on - strand at 44.028 kb on - strand, within EX31_RS14310 at 44.064 kb on - strand, within EX31_RS14310 at 44.089 kb on - strand, within EX31_RS14310 at 44.097 kb on - strand, within EX31_RS14310 at 44.132 kb on - strand, within EX31_RS14310 at 44.132 kb on - strand, within EX31_RS14310 at 44.174 kb on + strand, within EX31_RS14310 at 44.308 kb on - strand, within EX31_RS14310 at 44.379 kb on + strand, within EX31_RS14310 at 44.386 kb on - strand, within EX31_RS14310 at 44.468 kb on + strand, within EX31_RS14310 at 44.469 kb on - strand, within EX31_RS14310 at 44.469 kb on - strand, within EX31_RS14310 at 44.469 kb on - strand, within EX31_RS14310 at 44.469 kb on - strand, within EX31_RS14310 at 44.559 kb on - strand, within EX31_RS14310 at 44.559 kb on - strand, within EX31_RS14310 at 44.559 kb on - strand, within EX31_RS14310 at 44.559 kb on - strand, within EX31_RS14310 at 44.596 kb on - strand, within EX31_RS14310 at 44.611 kb on - strand, within EX31_RS14310 at 44.629 kb on - strand, within EX31_RS14310
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 41,767 + +1.0 41,789 + +0.9 41,805 + -0.1 41,806 - +0.8 41,806 - -1.2 41,807 + -0.0 41,807 + -0.1 41,807 + +0.2 41,807 + +1.2 41,807 + -0.1 41,807 + -0.3 41,808 - -1.8 41,821 + +0.0 41,821 + -0.3 41,821 + +0.1 41,821 + +0.1 41,822 - -0.0 41,830 + +1.0 41,830 + +1.1 41,830 + +0.6 41,830 + +0.8 42,638 - -0.3 42,638 - -1.0 42,647 + -0.3 42,647 + -0.6 42,707 - +0.1 42,707 - -0.1 42,874 + EX31_RS14305 0.16 +0.5 42,910 + EX31_RS14305 0.19 -0.5 42,911 - EX31_RS14305 0.20 +0.8 42,916 + EX31_RS14305 0.20 +1.0 42,916 + EX31_RS14305 0.20 -0.9 42,916 + EX31_RS14305 0.20 +0.1 42,916 + EX31_RS14305 0.20 +0.8 42,916 + EX31_RS14305 0.20 -0.7 42,916 + EX31_RS14305 0.20 -0.9 42,916 + EX31_RS14305 0.20 +0.0 42,916 + EX31_RS14305 0.20 +0.7 42,917 - EX31_RS14305 0.20 +1.0 42,917 - EX31_RS14305 0.20 +0.1 42,917 - EX31_RS14305 0.20 +0.0 42,917 - EX31_RS14305 0.20 -0.3 42,917 - EX31_RS14305 0.20 -0.6 42,917 - EX31_RS14305 0.20 +1.2 42,926 - EX31_RS14305 0.21 +0.6 42,941 + EX31_RS14305 0.23 -1.0 42,941 + EX31_RS14305 0.23 -0.3 42,942 - EX31_RS14305 0.23 +0.8 42,956 + EX31_RS14305 0.24 -0.5 42,956 + EX31_RS14305 0.24 -0.9 42,968 + EX31_RS14305 0.25 -0.3 43,031 + EX31_RS14305 0.32 -1.1 43,031 + EX31_RS14305 0.32 -1.2 43,032 - EX31_RS14305 0.32 +0.6 43,115 + EX31_RS14305 0.40 -0.6 43,115 + EX31_RS14305 0.40 -0.2 43,115 + EX31_RS14305 0.40 -0.0 43,116 - EX31_RS14305 0.40 -1.7 43,118 + EX31_RS14305 0.41 -2.2 43,119 - EX31_RS14305 0.41 -0.2 43,119 - EX31_RS14305 0.41 -0.5 43,160 + EX31_RS14305 0.45 -0.9 43,161 - EX31_RS14305 0.45 -1.7 43,163 - EX31_RS14305 0.45 -0.2 43,163 - EX31_RS14305 0.45 -0.2 43,163 - EX31_RS14305 0.45 -0.3 43,163 - EX31_RS14305 0.45 +0.5 43,240 + EX31_RS14305 0.53 -0.1 43,241 - EX31_RS14305 0.53 -0.7 43,241 - EX31_RS14305 0.53 +0.8 43,268 + EX31_RS14305 0.56 -0.7 43,268 + EX31_RS14305 0.56 +0.5 43,274 + EX31_RS14305 0.56 +0.1 43,274 + EX31_RS14305 0.56 -0.7 43,280 + EX31_RS14305 0.57 +0.7 43,280 + EX31_RS14305 0.57 -0.6 43,281 - EX31_RS14305 0.57 -0.2 43,281 - EX31_RS14305 0.57 -1.5 43,281 - EX31_RS14305 0.57 +0.1 43,290 + EX31_RS14305 0.58 -0.1 43,372 + EX31_RS14305 0.66 -0.1 43,372 + EX31_RS14305 0.66 +1.4 43,373 - EX31_RS14305 0.66 -1.1 43,436 + EX31_RS14305 0.73 -0.2 43,436 + EX31_RS14305 0.73 -0.3 43,437 - EX31_RS14305 0.73 +1.7 43,450 + EX31_RS14305 0.74 +0.5 43,450 + EX31_RS14305 0.74 -1.0 43,450 + EX31_RS14305 0.74 +1.0 43,451 - EX31_RS14305 0.74 -0.2 43,451 - EX31_RS14305 0.74 -0.1 43,468 - EX31_RS14305 0.76 +1.0 43,490 + EX31_RS14305 0.78 +0.0 43,490 + EX31_RS14305 0.78 +1.3 43,490 + EX31_RS14305 0.78 -1.1 43,494 + EX31_RS14305 0.78 +0.6 43,495 - EX31_RS14305 0.79 +0.2 43,495 - EX31_RS14305 0.79 -2.2 43,495 - EX31_RS14305 0.79 +0.4 43,495 - EX31_RS14305 0.79 -0.8 43,502 + EX31_RS14305 0.79 -0.1 43,502 + EX31_RS14305 0.79 +1.7 43,502 + EX31_RS14305 0.79 +0.2 43,502 + EX31_RS14305 0.79 -0.1 43,502 + EX31_RS14305 0.79 -0.3 43,503 - EX31_RS14305 0.79 -1.2 43,503 - EX31_RS14305 0.79 +0.6 43,537 - EX31_RS14305 0.83 -0.7 43,547 + EX31_RS14305 0.84 -0.8 43,570 + EX31_RS14305 0.86 +1.2 43,571 - EX31_RS14305 0.86 +0.3 43,571 - EX31_RS14305 0.86 +0.4 43,574 + EX31_RS14305 0.87 -2.2 43,575 - EX31_RS14305 0.87 +0.3 43,589 + EX31_RS14305 0.88 -0.7 43,589 + EX31_RS14305 0.88 +0.0 43,589 + EX31_RS14305 0.88 +0.5 43,589 + EX31_RS14305 0.88 +0.1 43,589 + EX31_RS14305 0.88 +0.7 43,589 + EX31_RS14305 0.88 -1.0 43,589 + EX31_RS14305 0.88 -1.0 43,589 + EX31_RS14305 0.88 -0.5 43,589 + EX31_RS14305 0.88 -0.6 43,590 - EX31_RS14305 0.88 -0.2 43,590 - EX31_RS14305 0.88 +0.3 43,590 - EX31_RS14305 0.88 -0.5 43,590 - EX31_RS14305 0.88 +0.5 43,590 - EX31_RS14305 0.88 -0.6 43,590 - EX31_RS14305 0.88 -0.3 43,590 - EX31_RS14305 0.88 +0.2 43,590 - EX31_RS14305 0.88 -1.0 43,590 - EX31_RS14305 0.88 -1.3 43,590 - EX31_RS14305 0.88 -0.5 43,591 + EX31_RS14305 0.88 -0.7 43,591 + EX31_RS14305 0.88 +0.1 43,591 + EX31_RS14305 0.88 -0.2 43,591 + EX31_RS14305 0.88 -1.8 43,591 + EX31_RS14305 0.88 +0.0 43,591 + EX31_RS14305 0.88 +0.4 43,591 + EX31_RS14305 0.88 -2.6 43,592 - EX31_RS14305 0.88 +0.3 43,592 - EX31_RS14305 0.88 -0.3 43,592 - EX31_RS14305 0.88 -1.0 43,592 - EX31_RS14305 0.88 -0.2 43,592 - EX31_RS14305 0.88 -0.5 43,592 - EX31_RS14305 0.88 +1.0 43,592 - EX31_RS14305 0.88 -0.7 43,592 - EX31_RS14305 0.88 -0.1 43,592 - EX31_RS14305 0.88 +0.2 43,592 - EX31_RS14305 0.88 +0.5 43,592 - EX31_RS14305 0.88 -0.6 43,592 - EX31_RS14305 0.88 +0.1 43,592 - EX31_RS14305 0.88 +0.3 43,592 - EX31_RS14305 0.88 -1.1 43,600 + EX31_RS14305 0.89 -0.5 43,600 + EX31_RS14305 0.89 +1.2 43,600 + EX31_RS14305 0.89 -0.9 43,601 - EX31_RS14305 0.89 -0.6 43,622 - -0.8 43,622 - -0.2 43,646 - -0.2 43,700 + -0.2 43,700 + -2.0 43,700 + -1.0 43,701 - -1.3 43,701 - -0.5 43,701 - -0.4 43,701 - -1.0 43,701 - -0.1 43,709 + +0.6 43,709 + +0.2 43,709 + -0.6 43,710 - +0.3 43,710 - +0.1 43,710 - -0.7 43,710 - -0.1 43,722 + +0.2 43,722 + -0.6 43,722 + +0.3 43,722 + +0.6 43,723 - -0.9 43,723 - -0.2 43,723 - -0.3 43,723 - +0.1 43,723 - -0.3 43,723 - +0.2 43,729 + +0.9 43,742 + +1.5 43,743 - -0.4 43,743 - +0.3 43,771 + -0.4 43,787 - -0.5 43,794 - +0.4 43,812 - -0.4 43,883 - +0.7 44,028 - EX31_RS14310 0.14 -1.6 44,064 - EX31_RS14310 0.18 -2.4 44,089 - EX31_RS14310 0.20 -2.4 44,097 - EX31_RS14310 0.21 -1.8 44,132 - EX31_RS14310 0.24 -1.8 44,132 - EX31_RS14310 0.24 -1.2 44,174 + EX31_RS14310 0.29 +1.0 44,308 - EX31_RS14310 0.42 -0.8 44,379 + EX31_RS14310 0.49 +0.2 44,386 - EX31_RS14310 0.50 -1.8 44,468 + EX31_RS14310 0.58 -0.5 44,469 - EX31_RS14310 0.59 -0.8 44,469 - EX31_RS14310 0.59 -2.4 44,469 - EX31_RS14310 0.59 -0.8 44,469 - EX31_RS14310 0.59 -1.8 44,559 - EX31_RS14310 0.68 +1.0 44,559 - EX31_RS14310 0.68 -1.2 44,559 - EX31_RS14310 0.68 -0.0 44,559 - EX31_RS14310 0.68 -0.6 44,596 - EX31_RS14310 0.71 -2.2 44,611 - EX31_RS14310 0.73 -1.2 44,629 - EX31_RS14310 0.75 -0.6
Or see this region's nucleotide sequence