Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS13905 and EX31_RS13910 are separated by 46 nucleotides EX31_RS13910 and EX31_RS13915 are separated by 456 nucleotides EX31_RS13915 and EX31_RS13920 are separated by 116 nucleotides
EX31_RS13905: EX31_RS13905 - Fe-S cluster assembly protein SufB, at 40,201 to 41,724
_RS13905
EX31_RS13910: EX31_RS13910 - Fe-S cluster assembly scaffold SufA, at 41,771 to 42,142
_RS13910
EX31_RS13915: EX31_RS13915 - hypothetical protein, at 42,599 to 43,003
_RS13915
EX31_RS13920: EX31_RS13920 - hotdog fold thioesterase, at 43,120 to 43,539
_RS13920
Position (kb) 41
42
43 Strain fitness (log2 ratio) -2
-1
0
1
2 at 41.751 kb on - strand at 41.751 kb on - strand at 41.773 kb on - strand at 42.163 kb on - strand at 42.163 kb on - strand at 42.182 kb on - strand at 42.182 kb on - strand at 42.182 kb on - strand at 42.196 kb on - strand at 42.196 kb on - strand at 42.237 kb on + strand at 42.282 kb on + strand at 42.282 kb on + strand at 42.282 kb on + strand at 42.283 kb on - strand at 42.283 kb on - strand at 42.364 kb on + strand at 42.371 kb on + strand at 42.373 kb on + strand at 42.387 kb on + strand at 42.406 kb on + strand at 42.406 kb on + strand at 42.406 kb on + strand at 42.407 kb on - strand at 42.409 kb on + strand at 42.409 kb on + strand at 42.409 kb on + strand at 42.410 kb on - strand at 42.410 kb on - strand at 42.411 kb on + strand at 42.412 kb on - strand at 42.433 kb on + strand at 42.434 kb on - strand at 42.486 kb on + strand at 42.487 kb on - strand at 42.490 kb on + strand at 42.490 kb on + strand at 42.490 kb on + strand at 42.491 kb on - strand at 42.491 kb on - strand at 42.491 kb on - strand at 42.544 kb on + strand at 42.544 kb on + strand at 42.545 kb on - strand at 42.545 kb on - strand at 42.545 kb on - strand at 42.545 kb on - strand at 42.545 kb on - strand at 42.545 kb on - strand at 42.612 kb on + strand at 42.645 kb on - strand, within EX31_RS13915 at 42.646 kb on + strand, within EX31_RS13915 at 42.646 kb on + strand, within EX31_RS13915 at 42.647 kb on - strand, within EX31_RS13915 at 42.647 kb on - strand, within EX31_RS13915 at 42.680 kb on + strand, within EX31_RS13915 at 42.680 kb on + strand, within EX31_RS13915 at 42.681 kb on - strand, within EX31_RS13915 at 42.797 kb on + strand, within EX31_RS13915 at 42.798 kb on - strand, within EX31_RS13915 at 42.798 kb on - strand, within EX31_RS13915 at 42.798 kb on - strand, within EX31_RS13915 at 42.798 kb on - strand, within EX31_RS13915 at 42.819 kb on + strand, within EX31_RS13915 at 42.819 kb on + strand, within EX31_RS13915 at 42.820 kb on - strand, within EX31_RS13915 at 42.820 kb on - strand, within EX31_RS13915 at 42.820 kb on - strand, within EX31_RS13915 at 42.820 kb on - strand, within EX31_RS13915 at 42.823 kb on + strand, within EX31_RS13915 at 42.907 kb on - strand, within EX31_RS13915 at 42.923 kb on + strand, within EX31_RS13915 at 42.927 kb on - strand, within EX31_RS13915 at 43.020 kb on + strand at 43.043 kb on - strand at 43.093 kb on - strand at 43.093 kb on - strand at 43.118 kb on + strand at 43.133 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 41,751 - +0.0 41,751 - -0.2 41,773 - +1.7 42,163 - +0.4 42,163 - -0.1 42,182 - +1.5 42,182 - +0.3 42,182 - +0.5 42,196 - +1.3 42,196 - +1.1 42,237 + +0.9 42,282 + +1.8 42,282 + +1.7 42,282 + +0.4 42,283 - -0.8 42,283 - +0.4 42,364 + +1.2 42,371 + +1.4 42,373 + +1.6 42,387 + +0.2 42,406 + -0.5 42,406 + +0.1 42,406 + -0.1 42,407 - -0.1 42,409 + +0.1 42,409 + +0.7 42,409 + +2.0 42,410 - +0.1 42,410 - +0.6 42,411 + +1.5 42,412 - +0.4 42,433 + +0.1 42,434 - -1.7 42,486 + -0.1 42,487 - +0.1 42,490 + +0.9 42,490 + +0.5 42,490 + +0.7 42,491 - +1.1 42,491 - +0.2 42,491 - +0.7 42,544 + -0.6 42,544 + -1.8 42,545 - +0.9 42,545 - -0.3 42,545 - -1.1 42,545 - -0.3 42,545 - +0.1 42,545 - +0.3 42,612 + +0.5 42,645 - EX31_RS13915 0.11 +0.4 42,646 + EX31_RS13915 0.12 -1.8 42,646 + EX31_RS13915 0.12 -1.0 42,647 - EX31_RS13915 0.12 +0.1 42,647 - EX31_RS13915 0.12 -0.8 42,680 + EX31_RS13915 0.20 +0.2 42,680 + EX31_RS13915 0.20 -0.4 42,681 - EX31_RS13915 0.20 +0.9 42,797 + EX31_RS13915 0.49 +0.3 42,798 - EX31_RS13915 0.49 +0.4 42,798 - EX31_RS13915 0.49 -0.3 42,798 - EX31_RS13915 0.49 +1.7 42,798 - EX31_RS13915 0.49 -0.8 42,819 + EX31_RS13915 0.54 +0.8 42,819 + EX31_RS13915 0.54 -0.1 42,820 - EX31_RS13915 0.55 -0.3 42,820 - EX31_RS13915 0.55 -0.6 42,820 - EX31_RS13915 0.55 -0.3 42,820 - EX31_RS13915 0.55 -0.6 42,823 + EX31_RS13915 0.55 +0.3 42,907 - EX31_RS13915 0.76 -0.2 42,923 + EX31_RS13915 0.80 -0.3 42,927 - EX31_RS13915 0.81 -0.6 43,020 + -0.2 43,043 - -0.4 43,093 - +0.0 43,093 - -1.1 43,118 + -0.1 43,133 - +0.1
Or see this region's nucleotide sequence