Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09845 and EX31_RS09850 are separated by 231 nucleotides EX31_RS09850 and EX31_RS09855 are separated by 12 nucleotides EX31_RS09855 and EX31_RS09860 are separated by 191 nucleotides EX31_RS09860 and EX31_RS09865 are separated by 94 nucleotides
EX31_RS09845: EX31_RS09845 - osmotically-inducible lipoprotein OsmB, at 177,268 to 177,486
_RS09845
EX31_RS09850: EX31_RS09850 - stress response translation initiation inhibitor YciH, at 177,718 to 178,047
_RS09850
EX31_RS09855: EX31_RS09855 - orotidine-5'-phosphate decarboxylase, at 178,060 to 178,788
_RS09855
EX31_RS09860: EX31_RS09860 - Arc family DNA-binding protein, at 178,980 to 179,267
_RS09860
EX31_RS09865: EX31_RS09865 - lipopolysaccharide assembly protein LapB, at 179,362 to 180,531
_RS09865
Position (kb) 178
179 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 177.077 kb on - strand at 177.095 kb on + strand at 177.095 kb on + strand at 177.183 kb on + strand at 177.206 kb on + strand at 177.206 kb on + strand at 177.207 kb on - strand at 177.226 kb on + strand at 177.226 kb on + strand at 177.230 kb on + strand at 177.283 kb on - strand at 177.351 kb on + strand, within EX31_RS09845 at 177.352 kb on - strand, within EX31_RS09845 at 177.428 kb on + strand, within EX31_RS09845 at 177.437 kb on + strand, within EX31_RS09845 at 177.437 kb on + strand, within EX31_RS09845 at 177.437 kb on + strand, within EX31_RS09845 at 177.437 kb on + strand, within EX31_RS09845 at 177.459 kb on + strand, within EX31_RS09845 at 177.460 kb on - strand, within EX31_RS09845 at 177.480 kb on + strand at 177.501 kb on + strand at 177.502 kb on - strand at 177.508 kb on + strand at 177.512 kb on + strand at 177.549 kb on - strand at 177.648 kb on - strand at 177.685 kb on - strand at 177.685 kb on - strand at 177.688 kb on - strand at 177.709 kb on - strand at 178.021 kb on + strand at 178.022 kb on - strand at 178.140 kb on - strand, within EX31_RS09855 at 178.140 kb on - strand, within EX31_RS09855 at 178.140 kb on - strand, within EX31_RS09855 at 178.180 kb on - strand, within EX31_RS09855 at 178.292 kb on - strand, within EX31_RS09855 at 178.292 kb on - strand, within EX31_RS09855 at 178.367 kb on - strand, within EX31_RS09855 at 178.367 kb on - strand, within EX31_RS09855 at 178.367 kb on - strand, within EX31_RS09855 at 178.549 kb on - strand, within EX31_RS09855 at 178.714 kb on - strand, within EX31_RS09855 at 178.830 kb on + strand at 178.833 kb on - strand at 178.854 kb on - strand at 178.880 kb on + strand at 178.881 kb on - strand at 178.881 kb on - strand at 178.881 kb on - strand at 178.881 kb on - strand at 178.886 kb on + strand at 178.887 kb on - strand at 178.887 kb on - strand at 178.958 kb on + strand at 178.959 kb on - strand at 178.963 kb on + strand at 178.963 kb on + strand at 178.990 kb on + strand at 179.265 kb on + strand at 179.265 kb on + strand at 179.266 kb on - strand at 179.266 kb on - strand at 179.283 kb on - strand at 179.285 kb on - strand at 179.285 kb on - strand at 179.300 kb on - strand at 179.304 kb on - strand at 179.348 kb on + strand at 179.349 kb on - strand at 179.364 kb on - strand at 179.364 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 177,077 - +0.9 177,095 + +0.6 177,095 + -0.4 177,183 + -0.0 177,206 + -0.6 177,206 + +0.1 177,207 - -2.1 177,226 + +0.1 177,226 + +3.6 177,230 + +0.6 177,283 - +1.6 177,351 + EX31_RS09845 0.38 -0.0 177,352 - EX31_RS09845 0.38 +1.2 177,428 + EX31_RS09845 0.73 +0.5 177,437 + EX31_RS09845 0.77 -1.0 177,437 + EX31_RS09845 0.77 -0.6 177,437 + EX31_RS09845 0.77 +0.4 177,437 + EX31_RS09845 0.77 -0.1 177,459 + EX31_RS09845 0.87 +0.6 177,460 - EX31_RS09845 0.88 -0.4 177,480 + +1.6 177,501 + +1.1 177,502 - -1.0 177,508 + -0.5 177,512 + -0.1 177,549 - +1.2 177,648 - +2.0 177,685 - -0.2 177,685 - +0.1 177,688 - +0.3 177,709 - +0.8 178,021 + +0.1 178,022 - +0.2 178,140 - EX31_RS09855 0.11 -3.8 178,140 - EX31_RS09855 0.11 -1.6 178,140 - EX31_RS09855 0.11 +0.6 178,180 - EX31_RS09855 0.16 -3.0 178,292 - EX31_RS09855 0.32 -2.6 178,292 - EX31_RS09855 0.32 -1.7 178,367 - EX31_RS09855 0.42 -2.1 178,367 - EX31_RS09855 0.42 -1.9 178,367 - EX31_RS09855 0.42 -1.2 178,549 - EX31_RS09855 0.67 -2.2 178,714 - EX31_RS09855 0.90 -1.6 178,830 + +1.6 178,833 - +1.7 178,854 - +1.1 178,880 + +1.1 178,881 - -0.6 178,881 - -1.2 178,881 - -0.5 178,881 - -0.4 178,886 + -0.0 178,887 - +0.6 178,887 - -1.9 178,958 + +0.9 178,959 - +0.8 178,963 + -0.2 178,963 + -0.0 178,990 + -2.1 179,265 + +0.6 179,265 + +0.6 179,266 - +0.3 179,266 - +0.6 179,283 - +2.6 179,285 - +0.6 179,285 - -1.4 179,300 - -1.0 179,304 - -0.4 179,348 + -1.3 179,349 - +0.3 179,364 - +1.1 179,364 - +1.9
Or see this region's nucleotide sequence