Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02505 and EX31_RS02510 are separated by 9 nucleotides EX31_RS02510 and EX31_RS02515 overlap by 44 nucleotides EX31_RS02515 and EX31_RS02520 overlap by 8 nucleotides
EX31_RS02505: EX31_RS02505 - hypothetical protein, at 531,112 to 531,768
_RS02505
EX31_RS02510: EX31_RS02510 - 4'-phosphopantetheinyl transferase superfamily protein, at 531,778 to 532,467
_RS02510
EX31_RS02515: EX31_RS02515 - PAS domain-containing protein, at 532,424 to 533,737
_RS02515
EX31_RS02520: EX31_RS02520 - sigma-54-dependent Fis family transcriptional regulator, at 533,730 to 535,142
_RS02520
Position (kb)
532
533
534 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 531.608 kb on + strand, within EX31_RS02505 at 531.608 kb on + strand, within EX31_RS02505 at 531.608 kb on + strand, within EX31_RS02505 at 531.609 kb on - strand, within EX31_RS02505 at 531.645 kb on + strand, within EX31_RS02505 at 531.799 kb on - strand at 531.860 kb on + strand, within EX31_RS02510 at 531.860 kb on + strand, within EX31_RS02510 at 531.892 kb on + strand, within EX31_RS02510 at 531.892 kb on + strand, within EX31_RS02510 at 531.893 kb on - strand, within EX31_RS02510 at 531.893 kb on - strand, within EX31_RS02510 at 531.893 kb on - strand, within EX31_RS02510 at 531.913 kb on - strand, within EX31_RS02510 at 531.925 kb on + strand, within EX31_RS02510 at 531.925 kb on + strand, within EX31_RS02510 at 531.925 kb on + strand, within EX31_RS02510 at 531.981 kb on + strand, within EX31_RS02510 at 531.982 kb on - strand, within EX31_RS02510 at 532.211 kb on - strand, within EX31_RS02510 at 532.276 kb on + strand, within EX31_RS02510 at 532.276 kb on + strand, within EX31_RS02510 at 532.276 kb on + strand, within EX31_RS02510 at 532.277 kb on - strand, within EX31_RS02510 at 532.277 kb on - strand, within EX31_RS02510 at 532.277 kb on - strand, within EX31_RS02510 at 532.558 kb on + strand, within EX31_RS02515 at 532.561 kb on - strand, within EX31_RS02515 at 532.654 kb on + strand, within EX31_RS02515 at 533.021 kb on + strand, within EX31_RS02515 at 533.135 kb on - strand, within EX31_RS02515 at 533.136 kb on + strand, within EX31_RS02515 at 533.136 kb on + strand, within EX31_RS02515 at 533.136 kb on + strand, within EX31_RS02515 at 533.136 kb on + strand, within EX31_RS02515 at 533.136 kb on + strand, within EX31_RS02515 at 533.136 kb on + strand, within EX31_RS02515 at 533.137 kb on - strand, within EX31_RS02515 at 533.137 kb on - strand, within EX31_RS02515 at 533.137 kb on - strand, within EX31_RS02515 at 533.137 kb on - strand, within EX31_RS02515 at 533.137 kb on - strand, within EX31_RS02515 at 533.155 kb on + strand, within EX31_RS02515 at 533.156 kb on - strand, within EX31_RS02515 at 533.156 kb on - strand, within EX31_RS02515 at 533.156 kb on - strand, within EX31_RS02515 at 533.302 kb on + strand, within EX31_RS02515 at 533.303 kb on - strand, within EX31_RS02515 at 533.303 kb on - strand, within EX31_RS02515 at 533.414 kb on + strand, within EX31_RS02515 at 533.622 kb on + strand at 533.696 kb on + strand at 533.697 kb on - strand at 533.697 kb on - strand at 533.697 kb on - strand at 533.699 kb on + strand at 533.700 kb on - strand at 533.824 kb on + strand at 534.092 kb on + strand, within EX31_RS02520 at 534.097 kb on + strand, within EX31_RS02520 at 534.098 kb on - strand, within EX31_RS02520 at 534.098 kb on - strand, within EX31_RS02520 at 534.143 kb on + strand, within EX31_RS02520 at 534.388 kb on - strand, within EX31_RS02520 at 534.388 kb on - strand, within EX31_RS02520 at 534.388 kb on - strand, within EX31_RS02520 at 534.555 kb on - strand, within EX31_RS02520 at 534.557 kb on - strand, within EX31_RS02520 at 534.645 kb on + strand, within EX31_RS02520
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 531,608 + EX31_RS02505 0.75 +1.1 531,608 + EX31_RS02505 0.75 -0.4 531,608 + EX31_RS02505 0.75 +0.5 531,609 - EX31_RS02505 0.76 +1.1 531,645 + EX31_RS02505 0.81 -0.5 531,799 - -1.5 531,860 + EX31_RS02510 0.12 -1.2 531,860 + EX31_RS02510 0.12 +0.5 531,892 + EX31_RS02510 0.17 -0.6 531,892 + EX31_RS02510 0.17 +0.3 531,893 - EX31_RS02510 0.17 -0.7 531,893 - EX31_RS02510 0.17 -1.4 531,893 - EX31_RS02510 0.17 +1.1 531,913 - EX31_RS02510 0.20 -1.5 531,925 + EX31_RS02510 0.21 -0.1 531,925 + EX31_RS02510 0.21 -0.8 531,925 + EX31_RS02510 0.21 -0.4 531,981 + EX31_RS02510 0.29 +0.8 531,982 - EX31_RS02510 0.30 -0.1 532,211 - EX31_RS02510 0.63 +0.3 532,276 + EX31_RS02510 0.72 +0.3 532,276 + EX31_RS02510 0.72 +0.9 532,276 + EX31_RS02510 0.72 -0.1 532,277 - EX31_RS02510 0.72 -1.4 532,277 - EX31_RS02510 0.72 -0.2 532,277 - EX31_RS02510 0.72 +0.3 532,558 + EX31_RS02515 0.10 -2.5 532,561 - EX31_RS02515 0.10 -1.9 532,654 + EX31_RS02515 0.18 -1.7 533,021 + EX31_RS02515 0.45 -0.7 533,135 - EX31_RS02515 0.54 -2.0 533,136 + EX31_RS02515 0.54 -0.8 533,136 + EX31_RS02515 0.54 -2.6 533,136 + EX31_RS02515 0.54 -1.8 533,136 + EX31_RS02515 0.54 -1.3 533,136 + EX31_RS02515 0.54 -0.9 533,136 + EX31_RS02515 0.54 -1.5 533,137 - EX31_RS02515 0.54 -1.3 533,137 - EX31_RS02515 0.54 -1.1 533,137 - EX31_RS02515 0.54 -3.3 533,137 - EX31_RS02515 0.54 -2.3 533,137 - EX31_RS02515 0.54 -2.5 533,155 + EX31_RS02515 0.56 -2.8 533,156 - EX31_RS02515 0.56 -0.7 533,156 - EX31_RS02515 0.56 -2.0 533,156 - EX31_RS02515 0.56 -1.8 533,302 + EX31_RS02515 0.67 -2.7 533,303 - EX31_RS02515 0.67 -2.4 533,303 - EX31_RS02515 0.67 -1.3 533,414 + EX31_RS02515 0.75 -3.8 533,622 + -2.5 533,696 + -2.3 533,697 - -2.4 533,697 - -0.1 533,697 - -1.7 533,699 + -1.1 533,700 - -1.7 533,824 + -1.5 534,092 + EX31_RS02520 0.26 -1.8 534,097 + EX31_RS02520 0.26 -1.7 534,098 - EX31_RS02520 0.26 -3.7 534,098 - EX31_RS02520 0.26 -1.7 534,143 + EX31_RS02520 0.29 -2.8 534,388 - EX31_RS02520 0.47 -2.1 534,388 - EX31_RS02520 0.47 -1.9 534,388 - EX31_RS02520 0.47 -1.4 534,555 - EX31_RS02520 0.58 -2.7 534,557 - EX31_RS02520 0.59 -2.3 534,645 + EX31_RS02520 0.65 -1.8
Or see this region's nucleotide sequence