Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00720 and EX31_RS00725 are separated by 554 nucleotides EX31_RS00725 and EX31_RS00730 are separated by 18 nucleotides
EX31_RS00720: EX31_RS00720 - hypothetical protein, at 153,775 to 154,152
_RS00720
EX31_RS00725: EX31_RS00725 - diaminobutyrate--2-oxoglutarate transaminase, at 154,707 to 156,092
_RS00725
EX31_RS00730: EX31_RS00730 - aspartate aminotransferase family protein, at 156,111 to 157,583
_RS00730
Position (kb) 154
155
156
157 Strain fitness (log2 ratio) -2
-1
0
1
2 at 153.735 kb on + strand at 153.735 kb on + strand at 153.736 kb on - strand at 153.736 kb on - strand at 153.736 kb on - strand at 153.802 kb on - strand at 153.952 kb on + strand, within EX31_RS00720 at 154.096 kb on + strand, within EX31_RS00720 at 154.096 kb on + strand, within EX31_RS00720 at 154.106 kb on + strand, within EX31_RS00720 at 154.106 kb on + strand, within EX31_RS00720 at 154.107 kb on - strand, within EX31_RS00720 at 154.107 kb on - strand, within EX31_RS00720 at 154.108 kb on + strand, within EX31_RS00720 at 154.108 kb on + strand, within EX31_RS00720 at 154.109 kb on - strand, within EX31_RS00720 at 154.175 kb on + strand at 154.176 kb on - strand at 154.176 kb on - strand at 154.177 kb on + strand at 154.177 kb on + strand at 154.177 kb on + strand at 154.178 kb on - strand at 154.180 kb on - strand at 154.194 kb on - strand at 154.203 kb on - strand at 154.205 kb on + strand at 154.205 kb on + strand at 154.239 kb on - strand at 154.243 kb on + strand at 154.243 kb on + strand at 154.244 kb on - strand at 154.244 kb on - strand at 154.244 kb on - strand at 154.256 kb on - strand at 154.280 kb on + strand at 154.280 kb on + strand at 154.295 kb on + strand at 154.299 kb on - strand at 154.311 kb on - strand at 154.312 kb on + strand at 154.312 kb on + strand at 154.313 kb on - strand at 154.328 kb on + strand at 154.328 kb on + strand at 154.329 kb on - strand at 154.387 kb on + strand at 154.399 kb on + strand at 154.435 kb on + strand at 154.435 kb on + strand at 154.435 kb on + strand at 154.435 kb on + strand at 154.436 kb on - strand at 154.436 kb on - strand at 154.436 kb on - strand at 154.436 kb on - strand at 154.436 kb on - strand at 154.436 kb on - strand at 154.488 kb on + strand at 154.489 kb on - strand at 154.492 kb on - strand at 154.578 kb on - strand at 154.616 kb on + strand at 154.616 kb on + strand at 154.616 kb on + strand at 154.616 kb on + strand at 154.618 kb on + strand at 154.618 kb on + strand at 154.618 kb on + strand at 154.618 kb on + strand at 154.618 kb on + strand at 154.619 kb on - strand at 154.636 kb on + strand at 154.653 kb on + strand at 154.663 kb on + strand at 154.681 kb on + strand at 154.681 kb on + strand at 154.681 kb on + strand at 154.681 kb on + strand at 154.681 kb on + strand at 154.681 kb on + strand at 154.684 kb on + strand at 154.697 kb on + strand at 154.706 kb on + strand at 154.773 kb on + strand at 154.774 kb on - strand at 154.823 kb on + strand at 154.823 kb on + strand at 154.823 kb on + strand at 154.823 kb on + strand at 154.823 kb on + strand at 154.823 kb on + strand at 154.931 kb on + strand, within EX31_RS00725 at 154.931 kb on + strand, within EX31_RS00725 at 154.931 kb on + strand, within EX31_RS00725 at 154.931 kb on + strand, within EX31_RS00725 at 154.931 kb on + strand, within EX31_RS00725 at 154.931 kb on + strand, within EX31_RS00725 at 154.932 kb on - strand, within EX31_RS00725 at 154.970 kb on + strand, within EX31_RS00725 at 154.987 kb on + strand, within EX31_RS00725 at 154.987 kb on + strand, within EX31_RS00725 at 154.987 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.991 kb on + strand, within EX31_RS00725 at 154.992 kb on - strand, within EX31_RS00725 at 154.992 kb on - strand, within EX31_RS00725 at 154.992 kb on - strand, within EX31_RS00725 at 154.992 kb on - strand, within EX31_RS00725 at 154.992 kb on - strand, within EX31_RS00725 at 155.034 kb on + strand, within EX31_RS00725 at 155.070 kb on + strand, within EX31_RS00725 at 155.070 kb on + strand, within EX31_RS00725 at 155.156 kb on + strand, within EX31_RS00725 at 155.267 kb on + strand, within EX31_RS00725 at 155.267 kb on + strand, within EX31_RS00725 at 155.268 kb on - strand, within EX31_RS00725 at 155.268 kb on - strand, within EX31_RS00725 at 155.274 kb on + strand, within EX31_RS00725 at 155.332 kb on + strand, within EX31_RS00725 at 155.332 kb on + strand, within EX31_RS00725 at 155.340 kb on + strand, within EX31_RS00725 at 155.576 kb on + strand, within EX31_RS00725 at 155.576 kb on + strand, within EX31_RS00725 at 155.630 kb on + strand, within EX31_RS00725 at 156.047 kb on + strand at 156.099 kb on + strand at 156.225 kb on + strand at 156.225 kb on + strand at 156.225 kb on + strand at 156.225 kb on + strand at 156.225 kb on + strand at 156.225 kb on + strand at 156.225 kb on + strand at 156.260 kb on + strand, within EX31_RS00730 at 156.260 kb on + strand, within EX31_RS00730 at 156.324 kb on + strand, within EX31_RS00730 at 156.440 kb on + strand, within EX31_RS00730 at 156.440 kb on + strand, within EX31_RS00730 at 156.506 kb on + strand, within EX31_RS00730 at 156.506 kb on + strand, within EX31_RS00730 at 156.670 kb on + strand, within EX31_RS00730 at 156.670 kb on + strand, within EX31_RS00730 at 156.670 kb on + strand, within EX31_RS00730 at 156.981 kb on + strand, within EX31_RS00730 at 156.981 kb on + strand, within EX31_RS00730 at 156.981 kb on + strand, within EX31_RS00730 at 157.000 kb on + strand, within EX31_RS00730
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 153,735 + +1.5 153,735 + -0.3 153,736 - -1.0 153,736 - +2.3 153,736 - +0.4 153,802 - +0.6 153,952 + EX31_RS00720 0.47 +1.4 154,096 + EX31_RS00720 0.85 +0.1 154,096 + EX31_RS00720 0.85 +0.5 154,106 + EX31_RS00720 0.88 +1.2 154,106 + EX31_RS00720 0.88 -2.1 154,107 - EX31_RS00720 0.88 +0.4 154,107 - EX31_RS00720 0.88 -0.6 154,108 + EX31_RS00720 0.88 +0.7 154,108 + EX31_RS00720 0.88 -0.5 154,109 - EX31_RS00720 0.88 +0.9 154,175 + +1.4 154,176 - -1.5 154,176 - +0.5 154,177 + +0.7 154,177 + -0.4 154,177 + -0.2 154,178 - -0.9 154,180 - -1.1 154,194 - -1.9 154,203 - -0.4 154,205 + -0.9 154,205 + +0.4 154,239 - -0.2 154,243 + -0.1 154,243 + -0.6 154,244 - -0.6 154,244 - -0.5 154,244 - -1.2 154,256 - +0.6 154,280 + -0.1 154,280 + -0.5 154,295 + -1.1 154,299 - -1.2 154,311 - +0.1 154,312 + +0.4 154,312 + +0.4 154,313 - -0.0 154,328 + +1.1 154,328 + +1.4 154,329 - +0.8 154,387 + -2.3 154,399 + +0.5 154,435 + -0.4 154,435 + +0.6 154,435 + -0.6 154,435 + -0.2 154,436 - +0.7 154,436 - -0.6 154,436 - -0.4 154,436 - -1.2 154,436 - +0.9 154,436 - -0.3 154,488 + -0.1 154,489 - +0.0 154,492 - -1.0 154,578 - +0.0 154,616 + -1.0 154,616 + -0.2 154,616 + +0.5 154,616 + -0.9 154,618 + -0.8 154,618 + -0.1 154,618 + -1.4 154,618 + +0.8 154,618 + +0.1 154,619 - +0.9 154,636 + -0.5 154,653 + -0.1 154,663 + -0.1 154,681 + +0.1 154,681 + -0.4 154,681 + -0.6 154,681 + -0.5 154,681 + +0.5 154,681 + -1.4 154,684 + -0.0 154,697 + -0.2 154,706 + +0.2 154,773 + -0.5 154,774 - +1.2 154,823 + +0.2 154,823 + -0.2 154,823 + -0.0 154,823 + +1.1 154,823 + -0.4 154,823 + +0.0 154,931 + EX31_RS00725 0.16 +0.6 154,931 + EX31_RS00725 0.16 +1.0 154,931 + EX31_RS00725 0.16 +1.1 154,931 + EX31_RS00725 0.16 -1.5 154,931 + EX31_RS00725 0.16 +0.4 154,931 + EX31_RS00725 0.16 +0.9 154,932 - EX31_RS00725 0.16 -1.9 154,970 + EX31_RS00725 0.19 +0.4 154,987 + EX31_RS00725 0.20 -1.1 154,987 + EX31_RS00725 0.20 -1.1 154,987 + EX31_RS00725 0.20 -1.4 154,991 + EX31_RS00725 0.20 +0.1 154,991 + EX31_RS00725 0.20 -0.2 154,991 + EX31_RS00725 0.20 +0.4 154,991 + EX31_RS00725 0.20 -0.4 154,991 + EX31_RS00725 0.20 -1.4 154,991 + EX31_RS00725 0.20 -0.0 154,991 + EX31_RS00725 0.20 +0.1 154,991 + EX31_RS00725 0.20 -0.6 154,991 + EX31_RS00725 0.20 -0.0 154,991 + EX31_RS00725 0.20 -0.6 154,991 + EX31_RS00725 0.20 +1.0 154,991 + EX31_RS00725 0.20 +1.9 154,991 + EX31_RS00725 0.20 +0.5 154,991 + EX31_RS00725 0.20 +0.3 154,991 + EX31_RS00725 0.20 -1.2 154,991 + EX31_RS00725 0.20 +0.8 154,991 + EX31_RS00725 0.20 +0.4 154,991 + EX31_RS00725 0.20 -0.9 154,992 - EX31_RS00725 0.21 +1.5 154,992 - EX31_RS00725 0.21 +0.6 154,992 - EX31_RS00725 0.21 +1.5 154,992 - EX31_RS00725 0.21 +0.6 154,992 - EX31_RS00725 0.21 +0.5 155,034 + EX31_RS00725 0.24 +0.1 155,070 + EX31_RS00725 0.26 +0.0 155,070 + EX31_RS00725 0.26 +0.5 155,156 + EX31_RS00725 0.32 -0.1 155,267 + EX31_RS00725 0.40 +0.3 155,267 + EX31_RS00725 0.40 +0.4 155,268 - EX31_RS00725 0.40 +1.9 155,268 - EX31_RS00725 0.40 +0.9 155,274 + EX31_RS00725 0.41 -1.4 155,332 + EX31_RS00725 0.45 +1.3 155,332 + EX31_RS00725 0.45 +0.5 155,340 + EX31_RS00725 0.46 -1.0 155,576 + EX31_RS00725 0.63 +0.4 155,576 + EX31_RS00725 0.63 -0.4 155,630 + EX31_RS00725 0.67 +0.1 156,047 + +0.4 156,099 + -1.2 156,225 + -1.4 156,225 + -0.6 156,225 + +1.9 156,225 + -0.8 156,225 + -0.1 156,225 + +0.4 156,225 + +1.1 156,260 + EX31_RS00730 0.10 +0.1 156,260 + EX31_RS00730 0.10 -1.3 156,324 + EX31_RS00730 0.14 -0.3 156,440 + EX31_RS00730 0.22 +0.3 156,440 + EX31_RS00730 0.22 -0.4 156,506 + EX31_RS00730 0.27 -0.5 156,506 + EX31_RS00730 0.27 -0.5 156,670 + EX31_RS00730 0.38 -0.1 156,670 + EX31_RS00730 0.38 +0.4 156,670 + EX31_RS00730 0.38 -0.7 156,981 + EX31_RS00730 0.59 -0.2 156,981 + EX31_RS00730 0.59 +0.3 156,981 + EX31_RS00730 0.59 +0.7 157,000 + EX31_RS00730 0.60 +1.9
Or see this region's nucleotide sequence