Experiment: R2A with perchlorate 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11020 and CA265_RS11025 are separated by 251 nucleotides CA265_RS11025 and CA265_RS11030 are separated by 57 nucleotides CA265_RS11030 and CA265_RS11035 are separated by 160 nucleotides CA265_RS11035 and CA265_RS11040 are separated by 224 nucleotides CA265_RS11040 and CA265_RS11045 are separated by 7 nucleotides
CA265_RS11020: CA265_RS11020 - hypothetical protein, at 2,704,968 to 2,708,525
_RS11020
CA265_RS11025: CA265_RS11025 - hypothetical protein, at 2,708,777 to 2,708,989
_RS11025
CA265_RS11030: CA265_RS11030 - twin-arginine translocase TatA/TatE family subunit, at 2,709,047 to 2,709,187
_RS11030
CA265_RS11035: CA265_RS11035 - phosphohydrolase, at 2,709,348 to 2,710,202
_RS11035
CA265_RS11040: CA265_RS11040 - hypothetical protein, at 2,710,427 to 2,711,080
_RS11040
CA265_RS11045: CA265_RS11045 - ABC transporter, at 2,711,088 to 2,712,386
_RS11045
Position (kb)
2709
2710
2711 Strain fitness (log2 ratio)
-1
0
1 at 2708.400 kb on - strand at 2708.400 kb on - strand at 2708.400 kb on - strand at 2708.400 kb on - strand at 2708.423 kb on - strand at 2708.581 kb on + strand at 2708.582 kb on - strand at 2708.675 kb on + strand at 2708.704 kb on - strand at 2708.771 kb on - strand at 2708.860 kb on + strand, within CA265_RS11025 at 2708.861 kb on - strand, within CA265_RS11025 at 2708.910 kb on - strand, within CA265_RS11025 at 2709.048 kb on + strand at 2709.049 kb on - strand at 2709.053 kb on - strand at 2709.162 kb on - strand, within CA265_RS11030 at 2709.187 kb on + strand at 2709.187 kb on + strand at 2709.195 kb on + strand at 2709.196 kb on - strand at 2709.207 kb on + strand at 2709.207 kb on + strand at 2709.209 kb on + strand at 2709.294 kb on + strand at 2709.295 kb on - strand at 2709.318 kb on + strand at 2709.408 kb on - strand at 2709.464 kb on - strand, within CA265_RS11035 at 2709.522 kb on + strand, within CA265_RS11035 at 2709.580 kb on - strand, within CA265_RS11035 at 2709.584 kb on + strand, within CA265_RS11035 at 2709.597 kb on - strand, within CA265_RS11035 at 2709.602 kb on + strand, within CA265_RS11035 at 2709.602 kb on + strand, within CA265_RS11035 at 2709.602 kb on + strand, within CA265_RS11035 at 2709.603 kb on - strand, within CA265_RS11035 at 2709.603 kb on - strand, within CA265_RS11035 at 2709.839 kb on - strand, within CA265_RS11035 at 2709.856 kb on - strand, within CA265_RS11035 at 2709.856 kb on - strand, within CA265_RS11035 at 2709.908 kb on - strand, within CA265_RS11035 at 2709.921 kb on - strand, within CA265_RS11035 at 2709.968 kb on + strand, within CA265_RS11035 at 2710.011 kb on + strand, within CA265_RS11035 at 2710.011 kb on + strand, within CA265_RS11035 at 2710.134 kb on - strand at 2710.211 kb on - strand at 2710.228 kb on - strand at 2710.284 kb on - strand at 2710.304 kb on + strand at 2710.348 kb on + strand at 2710.348 kb on + strand at 2710.407 kb on + strand at 2710.413 kb on + strand at 2710.424 kb on + strand at 2710.426 kb on + strand at 2710.427 kb on - strand at 2710.461 kb on + strand at 2710.462 kb on - strand at 2710.462 kb on - strand at 2710.504 kb on + strand, within CA265_RS11040 at 2710.573 kb on - strand, within CA265_RS11040 at 2710.573 kb on - strand, within CA265_RS11040 at 2710.647 kb on - strand, within CA265_RS11040 at 2710.649 kb on - strand, within CA265_RS11040 at 2710.679 kb on + strand, within CA265_RS11040 at 2710.680 kb on - strand, within CA265_RS11040 at 2710.680 kb on - strand, within CA265_RS11040 at 2710.685 kb on + strand, within CA265_RS11040 at 2710.724 kb on + strand, within CA265_RS11040 at 2710.725 kb on - strand, within CA265_RS11040 at 2710.756 kb on + strand, within CA265_RS11040 at 2710.813 kb on + strand, within CA265_RS11040 at 2710.827 kb on + strand, within CA265_RS11040 at 2710.832 kb on - strand, within CA265_RS11040 at 2710.865 kb on - strand, within CA265_RS11040 at 2711.014 kb on + strand, within CA265_RS11040 at 2711.079 kb on - strand at 2711.114 kb on + strand at 2711.120 kb on + strand at 2711.172 kb on + strand at 2711.175 kb on - strand at 2711.175 kb on - strand at 2711.178 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with perchlorate 25 mM remove 2,708,400 - -0.6 2,708,400 - -0.3 2,708,400 - -0.2 2,708,400 - -0.2 2,708,423 - +0.3 2,708,581 + -0.4 2,708,582 - +0.3 2,708,675 + +0.1 2,708,704 - +0.3 2,708,771 - +0.5 2,708,860 + CA265_RS11025 0.39 -1.1 2,708,861 - CA265_RS11025 0.39 -1.4 2,708,910 - CA265_RS11025 0.62 -1.1 2,709,048 + +0.7 2,709,049 - +0.2 2,709,053 - +0.3 2,709,162 - CA265_RS11030 0.82 +0.4 2,709,187 + +0.2 2,709,187 + -0.1 2,709,195 + +0.1 2,709,196 - -0.1 2,709,207 + +0.6 2,709,207 + +0.1 2,709,209 + +0.3 2,709,294 + +0.1 2,709,295 - -0.0 2,709,318 + -0.1 2,709,408 - -0.6 2,709,464 - CA265_RS11035 0.14 -0.5 2,709,522 + CA265_RS11035 0.20 -0.4 2,709,580 - CA265_RS11035 0.27 -0.2 2,709,584 + CA265_RS11035 0.28 +0.0 2,709,597 - CA265_RS11035 0.29 -0.7 2,709,602 + CA265_RS11035 0.30 -1.4 2,709,602 + CA265_RS11035 0.30 -0.2 2,709,602 + CA265_RS11035 0.30 +0.2 2,709,603 - CA265_RS11035 0.30 -0.6 2,709,603 - CA265_RS11035 0.30 +0.0 2,709,839 - CA265_RS11035 0.57 -0.1 2,709,856 - CA265_RS11035 0.59 -0.9 2,709,856 - CA265_RS11035 0.59 +1.6 2,709,908 - CA265_RS11035 0.65 -0.1 2,709,921 - CA265_RS11035 0.67 -1.1 2,709,968 + CA265_RS11035 0.73 +0.4 2,710,011 + CA265_RS11035 0.78 +1.2 2,710,011 + CA265_RS11035 0.78 +0.0 2,710,134 - -0.3 2,710,211 - +0.3 2,710,228 - -0.7 2,710,284 - -0.4 2,710,304 + -0.4 2,710,348 + +0.7 2,710,348 + -0.2 2,710,407 + +0.1 2,710,413 + -0.7 2,710,424 + -0.3 2,710,426 + -0.7 2,710,427 - -0.2 2,710,461 + +0.1 2,710,462 - -1.0 2,710,462 - -1.2 2,710,504 + CA265_RS11040 0.12 +0.3 2,710,573 - CA265_RS11040 0.22 +0.2 2,710,573 - CA265_RS11040 0.22 +0.5 2,710,647 - CA265_RS11040 0.34 -0.6 2,710,649 - CA265_RS11040 0.34 -1.6 2,710,679 + CA265_RS11040 0.39 -0.8 2,710,680 - CA265_RS11040 0.39 +0.2 2,710,680 - CA265_RS11040 0.39 -0.3 2,710,685 + CA265_RS11040 0.39 -0.0 2,710,724 + CA265_RS11040 0.45 -0.6 2,710,725 - CA265_RS11040 0.46 -0.3 2,710,756 + CA265_RS11040 0.50 +0.5 2,710,813 + CA265_RS11040 0.59 -0.8 2,710,827 + CA265_RS11040 0.61 +0.8 2,710,832 - CA265_RS11040 0.62 -1.3 2,710,865 - CA265_RS11040 0.67 +1.5 2,711,014 + CA265_RS11040 0.90 -0.7 2,711,079 - +0.2 2,711,114 + +0.1 2,711,120 + +0.4 2,711,172 + -0.2 2,711,175 - -0.4 2,711,175 - -0.5 2,711,178 - -0.5
Or see this region's nucleotide sequence