Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 10 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06300 and MMP_RS06305 are separated by 115 nucleotides MMP_RS06305 and MMP_RS06310 are separated by 19 nucleotides MMP_RS06310 and MMP_RS06315 are separated by 112 nucleotides MMP_RS06315 and MMP_RS06320 are separated by 22 nucleotides
MMP_RS06300: MMP_RS06300 - ABC transporter substrate-binding protein, at 1,213,682 to 1,214,446
_RS06300
MMP_RS06305: MMP_RS06305 - transporter substrate-binding domain-containing protein, at 1,214,562 to 1,215,326
_RS06305
MMP_RS06310: MMP_RS06310 - NAD(P)/FAD-dependent oxidoreductase, at 1,215,346 to 1,216,503
_RS06310
MMP_RS06315: MMP_RS06315 - cobalt-precorrin-7 (C(5))-methyltransferase, at 1,216,616 to 1,217,242
_RS06315
MMP_RS06320: MMP_RS06320 - hypothetical protein, at 1,217,265 to 1,217,903
_RS06320
Position (kb)
1215
1216
1217 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1214.539 kb on + strand at 1214.539 kb on + strand at 1214.546 kb on + strand at 1214.678 kb on + strand, within MMP_RS06305 at 1214.703 kb on - strand, within MMP_RS06305 at 1214.704 kb on + strand, within MMP_RS06305 at 1214.847 kb on + strand, within MMP_RS06305 at 1215.060 kb on - strand, within MMP_RS06305 at 1215.159 kb on + strand, within MMP_RS06305 at 1215.175 kb on + strand, within MMP_RS06305 at 1215.218 kb on - strand, within MMP_RS06305 at 1215.258 kb on + strand at 1215.259 kb on - strand at 1215.484 kb on - strand, within MMP_RS06310 at 1215.648 kb on + strand, within MMP_RS06310 at 1215.664 kb on - strand, within MMP_RS06310 at 1215.895 kb on + strand, within MMP_RS06310 at 1215.928 kb on - strand, within MMP_RS06310 at 1216.012 kb on + strand, within MMP_RS06310 at 1216.100 kb on + strand, within MMP_RS06310 at 1216.128 kb on - strand, within MMP_RS06310 at 1216.129 kb on + strand, within MMP_RS06310 at 1216.192 kb on + strand, within MMP_RS06310 at 1216.506 kb on - strand at 1216.550 kb on - strand at 1216.550 kb on - strand at 1216.553 kb on + strand at 1216.555 kb on - strand at 1216.572 kb on - strand at 1216.573 kb on + strand at 1216.573 kb on + strand at 1216.998 kb on - strand, within MMP_RS06315 at 1216.999 kb on + strand, within MMP_RS06315 at 1217.233 kb on - strand at 1217.236 kb on + strand at 1217.386 kb on - strand, within MMP_RS06320 at 1217.391 kb on + strand, within MMP_RS06320 at 1217.396 kb on - strand, within MMP_RS06320 at 1217.396 kb on - strand, within MMP_RS06320 at 1217.397 kb on + strand, within MMP_RS06320 at 1217.401 kb on - strand, within MMP_RS06320 at 1217.472 kb on - strand, within MMP_RS06320
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 10 generations remove 1,214,539 + +0.1 1,214,539 + -0.3 1,214,546 + +0.1 1,214,678 + MMP_RS06305 0.15 +0.6 1,214,703 - MMP_RS06305 0.18 -0.3 1,214,704 + MMP_RS06305 0.19 +0.2 1,214,847 + MMP_RS06305 0.37 +0.0 1,215,060 - MMP_RS06305 0.65 +0.4 1,215,159 + MMP_RS06305 0.78 +0.3 1,215,175 + MMP_RS06305 0.80 +0.1 1,215,218 - MMP_RS06305 0.86 +0.2 1,215,258 + -0.4 1,215,259 - -0.0 1,215,484 - MMP_RS06310 0.12 -2.4 1,215,648 + MMP_RS06310 0.26 -0.7 1,215,664 - MMP_RS06310 0.27 -1.8 1,215,895 + MMP_RS06310 0.47 -2.5 1,215,928 - MMP_RS06310 0.50 -2.2 1,216,012 + MMP_RS06310 0.58 -1.3 1,216,100 + MMP_RS06310 0.65 -3.6 1,216,128 - MMP_RS06310 0.68 -2.4 1,216,129 + MMP_RS06310 0.68 -0.6 1,216,192 + MMP_RS06310 0.73 +0.6 1,216,506 - -2.7 1,216,550 - -1.6 1,216,550 - -1.7 1,216,553 + -3.7 1,216,555 - -3.2 1,216,572 - -1.6 1,216,573 + -4.0 1,216,573 + -1.7 1,216,998 - MMP_RS06315 0.61 -3.5 1,216,999 + MMP_RS06315 0.61 -4.0 1,217,233 - -1.3 1,217,236 + -3.0 1,217,386 - MMP_RS06320 0.19 -0.0 1,217,391 + MMP_RS06320 0.20 +1.2 1,217,396 - MMP_RS06320 0.21 -0.6 1,217,396 - MMP_RS06320 0.21 +0.2 1,217,397 + MMP_RS06320 0.21 -0.2 1,217,401 - MMP_RS06320 0.21 +0.1 1,217,472 - MMP_RS06320 0.32 -0.1
Or see this region's nucleotide sequence