Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24385 and EX31_RS24390 are separated by 223 nucleotides EX31_RS24390 and EX31_RS24395 are separated by 73 nucleotides EX31_RS24395 and EX31_RS24400 are separated by 600 nucleotides
EX31_RS24385: EX31_RS24385 - dihydroorotase, at 644,286 to 645,335
_RS24385
EX31_RS24390: EX31_RS24390 - antibiotic biosynthesis monooxygenase, at 645,559 to 645,846
_RS24390
EX31_RS24395: EX31_RS24395 - ribonuclease E, at 645,920 to 649,207
_RS24395
EX31_RS24400: EX31_RS24400 - 23S rRNA pseudouridine(955/2504/2580) synthase RluC, at 649,808 to 650,770
_RS24400
Position (kb) 645
646
647
648
649
650 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 644.948 kb on + strand, within EX31_RS24385 at 644.948 kb on + strand, within EX31_RS24385 at 644.948 kb on + strand, within EX31_RS24385 at 644.948 kb on + strand, within EX31_RS24385 at 644.949 kb on - strand, within EX31_RS24385 at 645.023 kb on - strand, within EX31_RS24385 at 645.098 kb on - strand, within EX31_RS24385 at 645.098 kb on - strand, within EX31_RS24385 at 645.098 kb on - strand, within EX31_RS24385 at 645.098 kb on - strand, within EX31_RS24385 at 645.122 kb on + strand, within EX31_RS24385 at 645.122 kb on + strand, within EX31_RS24385 at 645.147 kb on - strand, within EX31_RS24385 at 645.228 kb on + strand, within EX31_RS24385 at 645.284 kb on + strand at 645.285 kb on - strand at 645.416 kb on - strand at 645.421 kb on + strand at 645.448 kb on + strand at 645.458 kb on - strand at 645.501 kb on + strand at 645.529 kb on - strand at 645.635 kb on + strand, within EX31_RS24390 at 645.636 kb on - strand, within EX31_RS24390 at 645.659 kb on + strand, within EX31_RS24390 at 645.659 kb on + strand, within EX31_RS24390 at 645.659 kb on + strand, within EX31_RS24390 at 645.660 kb on - strand, within EX31_RS24390 at 645.673 kb on + strand, within EX31_RS24390 at 645.674 kb on - strand, within EX31_RS24390 at 645.696 kb on + strand, within EX31_RS24390 at 645.997 kb on + strand at 646.057 kb on - strand at 646.057 kb on - strand at 646.057 kb on - strand at 646.057 kb on - strand at 646.057 kb on - strand at 646.057 kb on - strand at 646.178 kb on - strand at 646.487 kb on + strand, within EX31_RS24395 at 646.556 kb on - strand, within EX31_RS24395 at 646.556 kb on - strand, within EX31_RS24395 at 646.588 kb on - strand, within EX31_RS24395 at 646.774 kb on + strand, within EX31_RS24395 at 646.829 kb on - strand, within EX31_RS24395 at 646.866 kb on - strand, within EX31_RS24395 at 646.866 kb on - strand, within EX31_RS24395 at 647.057 kb on - strand, within EX31_RS24395 at 647.057 kb on - strand, within EX31_RS24395 at 647.144 kb on - strand, within EX31_RS24395 at 647.393 kb on - strand, within EX31_RS24395 at 647.393 kb on - strand, within EX31_RS24395 at 649.226 kb on - strand at 649.234 kb on - strand at 649.341 kb on - strand at 649.341 kb on - strand at 649.341 kb on - strand at 649.341 kb on - strand at 649.369 kb on - strand at 649.369 kb on - strand at 649.369 kb on - strand at 649.425 kb on - strand at 649.425 kb on - strand at 649.497 kb on - strand at 649.497 kb on - strand at 649.497 kb on - strand at 649.566 kb on - strand at 649.566 kb on - strand at 649.596 kb on - strand at 649.643 kb on + strand at 649.644 kb on - strand at 649.648 kb on + strand at 649.648 kb on + strand at 649.648 kb on + strand at 649.648 kb on + strand at 649.648 kb on + strand at 649.648 kb on + strand at 649.649 kb on - strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.653 kb on - strand at 649.653 kb on - strand at 649.698 kb on - strand at 649.735 kb on + strand at 649.735 kb on + strand at 649.736 kb on - strand at 649.753 kb on + strand at 649.753 kb on + strand at 649.753 kb on + strand at 649.794 kb on + strand at 649.795 kb on - strand at 649.830 kb on + strand at 649.830 kb on + strand at 649.831 kb on - strand at 649.836 kb on + strand at 649.836 kb on + strand at 649.837 kb on - strand at 649.884 kb on + strand at 649.884 kb on + strand at 649.888 kb on + strand at 649.889 kb on - strand at 649.889 kb on - strand at 649.889 kb on - strand at 649.889 kb on - strand at 649.922 kb on + strand, within EX31_RS24400 at 649.922 kb on + strand, within EX31_RS24400 at 649.922 kb on + strand, within EX31_RS24400 at 649.923 kb on - strand, within EX31_RS24400 at 649.923 kb on - strand, within EX31_RS24400 at 649.923 kb on - strand, within EX31_RS24400 at 649.936 kb on + strand, within EX31_RS24400 at 649.953 kb on + strand, within EX31_RS24400 at 649.957 kb on + strand, within EX31_RS24400 at 649.957 kb on + strand, within EX31_RS24400 at 649.957 kb on + strand, within EX31_RS24400 at 649.958 kb on - strand, within EX31_RS24400 at 649.958 kb on - strand, within EX31_RS24400 at 649.969 kb on + strand, within EX31_RS24400 at 649.980 kb on - strand, within EX31_RS24400 at 649.981 kb on + strand, within EX31_RS24400 at 649.986 kb on + strand, within EX31_RS24400 at 649.986 kb on + strand, within EX31_RS24400 at 649.986 kb on + strand, within EX31_RS24400 at 649.986 kb on + strand, within EX31_RS24400 at 650.066 kb on - strand, within EX31_RS24400 at 650.094 kb on + strand, within EX31_RS24400 at 650.094 kb on + strand, within EX31_RS24400 at 650.094 kb on + strand, within EX31_RS24400 at 650.094 kb on + strand, within EX31_RS24400 at 650.094 kb on + strand, within EX31_RS24400 at 650.094 kb on + strand, within EX31_RS24400 at 650.094 kb on + strand, within EX31_RS24400 at 650.096 kb on + strand, within EX31_RS24400 at 650.096 kb on + strand, within EX31_RS24400 at 650.096 kb on + strand, within EX31_RS24400 at 650.096 kb on + strand, within EX31_RS24400 at 650.096 kb on + strand, within EX31_RS24400 at 650.096 kb on + strand, within EX31_RS24400 at 650.097 kb on - strand, within EX31_RS24400 at 650.122 kb on + strand, within EX31_RS24400 at 650.125 kb on + strand, within EX31_RS24400 at 650.126 kb on - strand, within EX31_RS24400 at 650.146 kb on - strand, within EX31_RS24400 at 650.163 kb on + strand, within EX31_RS24400
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 644,948 + EX31_RS24385 0.63 -1.3 644,948 + EX31_RS24385 0.63 +0.2 644,948 + EX31_RS24385 0.63 -0.6 644,948 + EX31_RS24385 0.63 -3.5 644,949 - EX31_RS24385 0.63 -1.3 645,023 - EX31_RS24385 0.70 -0.8 645,098 - EX31_RS24385 0.77 -2.9 645,098 - EX31_RS24385 0.77 -2.8 645,098 - EX31_RS24385 0.77 -1.7 645,098 - EX31_RS24385 0.77 -1.8 645,122 + EX31_RS24385 0.80 -2.3 645,122 + EX31_RS24385 0.80 -0.8 645,147 - EX31_RS24385 0.82 -1.1 645,228 + EX31_RS24385 0.90 -1.3 645,284 + -2.4 645,285 - -1.6 645,416 - -1.3 645,421 + -0.3 645,448 + +1.5 645,458 - -0.3 645,501 + +1.2 645,529 - -0.8 645,635 + EX31_RS24390 0.26 -1.0 645,636 - EX31_RS24390 0.27 -0.3 645,659 + EX31_RS24390 0.35 +0.6 645,659 + EX31_RS24390 0.35 +0.2 645,659 + EX31_RS24390 0.35 +0.2 645,660 - EX31_RS24390 0.35 -0.1 645,673 + EX31_RS24390 0.40 +0.4 645,674 - EX31_RS24390 0.40 +0.5 645,696 + EX31_RS24390 0.48 +1.0 645,997 + +1.0 646,057 - +2.3 646,057 - -1.0 646,057 - +0.3 646,057 - -1.3 646,057 - -1.4 646,057 - +1.7 646,178 - -2.0 646,487 + EX31_RS24395 0.17 +0.7 646,556 - EX31_RS24395 0.19 +0.3 646,556 - EX31_RS24395 0.19 -1.3 646,588 - EX31_RS24395 0.20 +0.2 646,774 + EX31_RS24395 0.26 +0.2 646,829 - EX31_RS24395 0.28 -1.4 646,866 - EX31_RS24395 0.29 -0.1 646,866 - EX31_RS24395 0.29 -3.0 647,057 - EX31_RS24395 0.35 -0.6 647,057 - EX31_RS24395 0.35 -0.3 647,144 - EX31_RS24395 0.37 -2.6 647,393 - EX31_RS24395 0.45 +0.2 647,393 - EX31_RS24395 0.45 -0.4 649,226 - +0.5 649,234 - -1.1 649,341 - -0.5 649,341 - +0.2 649,341 - -0.5 649,341 - +1.1 649,369 - +0.4 649,369 - -0.1 649,369 - -0.6 649,425 - +0.7 649,425 - -1.3 649,497 - +1.2 649,497 - +1.0 649,497 - +0.6 649,566 - +1.7 649,566 - -2.2 649,596 - -0.6 649,643 + -1.3 649,644 - -0.2 649,648 + -0.2 649,648 + -1.0 649,648 + +0.4 649,648 + -1.5 649,648 + +0.9 649,648 + -1.8 649,649 - +0.5 649,652 + +0.1 649,652 + -3.4 649,652 + -0.0 649,652 + +1.3 649,653 - -0.6 649,653 - +0.6 649,698 - +0.6 649,735 + -0.8 649,735 + +0.1 649,736 - +0.3 649,753 + -0.8 649,753 + -0.5 649,753 + -0.1 649,794 + +0.8 649,795 - +2.5 649,830 + +1.1 649,830 + -0.3 649,831 - -1.7 649,836 + +0.3 649,836 + -1.0 649,837 - +0.0 649,884 + +0.2 649,884 + +0.5 649,888 + -0.7 649,889 - +0.3 649,889 - -1.8 649,889 - -0.9 649,889 - -1.1 649,922 + EX31_RS24400 0.12 +0.2 649,922 + EX31_RS24400 0.12 -1.8 649,922 + EX31_RS24400 0.12 +0.0 649,923 - EX31_RS24400 0.12 +0.4 649,923 - EX31_RS24400 0.12 +1.2 649,923 - EX31_RS24400 0.12 +0.2 649,936 + EX31_RS24400 0.13 +0.7 649,953 + EX31_RS24400 0.15 -1.9 649,957 + EX31_RS24400 0.15 -0.6 649,957 + EX31_RS24400 0.15 -0.0 649,957 + EX31_RS24400 0.15 +0.2 649,958 - EX31_RS24400 0.16 -0.8 649,958 - EX31_RS24400 0.16 +2.2 649,969 + EX31_RS24400 0.17 +1.1 649,980 - EX31_RS24400 0.18 +1.4 649,981 + EX31_RS24400 0.18 +0.6 649,986 + EX31_RS24400 0.18 -2.0 649,986 + EX31_RS24400 0.18 +0.7 649,986 + EX31_RS24400 0.18 +0.9 649,986 + EX31_RS24400 0.18 +0.3 650,066 - EX31_RS24400 0.27 +0.4 650,094 + EX31_RS24400 0.30 +0.2 650,094 + EX31_RS24400 0.30 +0.2 650,094 + EX31_RS24400 0.30 +0.4 650,094 + EX31_RS24400 0.30 +1.4 650,094 + EX31_RS24400 0.30 -1.4 650,094 + EX31_RS24400 0.30 +1.0 650,094 + EX31_RS24400 0.30 +0.0 650,096 + EX31_RS24400 0.30 +0.7 650,096 + EX31_RS24400 0.30 -0.6 650,096 + EX31_RS24400 0.30 -0.3 650,096 + EX31_RS24400 0.30 +0.0 650,096 + EX31_RS24400 0.30 +0.6 650,096 + EX31_RS24400 0.30 +0.2 650,097 - EX31_RS24400 0.30 -1.1 650,122 + EX31_RS24400 0.33 -1.1 650,125 + EX31_RS24400 0.33 -0.4 650,126 - EX31_RS24400 0.33 -1.6 650,146 - EX31_RS24400 0.35 +1.3 650,163 + EX31_RS24400 0.37 -0.9
Or see this region's nucleotide sequence