Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18995 and EX31_RS19000 are separated by 203 nucleotides EX31_RS19000 and EX31_RS19005 are separated by 84 nucleotides EX31_RS19005 and EX31_RS19010 are separated by 112 nucleotides EX31_RS19010 and EX31_RS19015 are separated by 301 nucleotides
EX31_RS18995: EX31_RS18995 - 23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF, at 387,347 to 388,315
_RS18995
EX31_RS19000: EX31_RS19000 - flavin reductase family protein, at 388,519 to 389,148
_RS19000
EX31_RS19005: EX31_RS19005 - peptidase M3, at 389,233 to 391,095
_RS19005
EX31_RS19010: EX31_RS19010 - alkylphosphonate utilization protein, at 391,208 to 391,549
_RS19010
EX31_RS19015: EX31_RS19015 - DNA-binding protein YbiB, at 391,851 to 392,813
_RS19015
Position (kb) 389
390
391
392 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 388.354 kb on + strand at 388.354 kb on + strand at 388.373 kb on + strand at 388.373 kb on + strand at 388.373 kb on + strand at 388.374 kb on - strand at 388.374 kb on - strand at 388.374 kb on - strand at 388.378 kb on - strand at 388.395 kb on + strand at 388.396 kb on - strand at 388.443 kb on + strand at 388.444 kb on - strand at 388.464 kb on + strand at 388.478 kb on + strand at 388.478 kb on + strand at 388.478 kb on + strand at 388.478 kb on + strand at 388.478 kb on + strand at 388.478 kb on + strand at 388.478 kb on + strand at 388.478 kb on + strand at 388.478 kb on + strand at 388.478 kb on + strand at 388.479 kb on - strand at 388.479 kb on - strand at 388.479 kb on - strand at 388.479 kb on - strand at 388.487 kb on + strand at 388.488 kb on - strand at 388.488 kb on - strand at 388.488 kb on - strand at 388.501 kb on + strand at 388.501 kb on + strand at 388.502 kb on - strand at 388.502 kb on - strand at 388.537 kb on + strand at 388.538 kb on - strand at 388.541 kb on - strand at 388.541 kb on - strand at 388.541 kb on - strand at 388.543 kb on + strand at 388.543 kb on + strand at 388.544 kb on - strand at 388.544 kb on - strand at 388.661 kb on - strand, within EX31_RS19000 at 388.661 kb on - strand, within EX31_RS19000 at 388.661 kb on - strand, within EX31_RS19000 at 388.684 kb on + strand, within EX31_RS19000 at 388.741 kb on - strand, within EX31_RS19000 at 388.938 kb on + strand, within EX31_RS19000 at 388.938 kb on + strand, within EX31_RS19000 at 388.938 kb on + strand, within EX31_RS19000 at 388.938 kb on + strand, within EX31_RS19000 at 388.938 kb on + strand, within EX31_RS19000 at 388.938 kb on + strand, within EX31_RS19000 at 388.939 kb on - strand, within EX31_RS19000 at 388.939 kb on - strand, within EX31_RS19000 at 388.939 kb on - strand, within EX31_RS19000 at 388.975 kb on - strand, within EX31_RS19000 at 389.041 kb on + strand, within EX31_RS19000 at 389.069 kb on + strand, within EX31_RS19000 at 389.095 kb on + strand at 389.096 kb on - strand at 389.158 kb on + strand at 389.158 kb on + strand at 389.158 kb on + strand at 389.159 kb on - strand at 389.206 kb on - strand at 389.214 kb on + strand at 389.215 kb on - strand at 389.215 kb on - strand at 389.215 kb on - strand at 389.235 kb on - strand at 389.235 kb on - strand at 389.246 kb on + strand at 389.247 kb on - strand at 389.247 kb on - strand at 389.457 kb on - strand, within EX31_RS19005 at 389.497 kb on - strand, within EX31_RS19005 at 389.497 kb on - strand, within EX31_RS19005 at 389.532 kb on - strand, within EX31_RS19005 at 389.577 kb on - strand, within EX31_RS19005 at 389.721 kb on + strand, within EX31_RS19005 at 389.722 kb on - strand, within EX31_RS19005 at 389.807 kb on + strand, within EX31_RS19005 at 389.808 kb on - strand, within EX31_RS19005 at 389.866 kb on - strand, within EX31_RS19005 at 389.866 kb on - strand, within EX31_RS19005 at 389.919 kb on - strand, within EX31_RS19005 at 389.982 kb on - strand, within EX31_RS19005 at 389.982 kb on - strand, within EX31_RS19005 at 390.036 kb on + strand, within EX31_RS19005 at 390.037 kb on - strand, within EX31_RS19005 at 390.037 kb on - strand, within EX31_RS19005 at 390.078 kb on + strand, within EX31_RS19005 at 390.078 kb on + strand, within EX31_RS19005 at 390.079 kb on - strand, within EX31_RS19005 at 390.135 kb on + strand, within EX31_RS19005 at 390.135 kb on + strand, within EX31_RS19005 at 390.136 kb on - strand, within EX31_RS19005 at 390.136 kb on - strand, within EX31_RS19005 at 390.167 kb on + strand, within EX31_RS19005 at 390.193 kb on - strand, within EX31_RS19005 at 390.193 kb on - strand, within EX31_RS19005 at 390.284 kb on - strand, within EX31_RS19005 at 390.317 kb on + strand, within EX31_RS19005 at 390.318 kb on - strand, within EX31_RS19005 at 390.469 kb on + strand, within EX31_RS19005 at 390.498 kb on + strand, within EX31_RS19005 at 390.498 kb on + strand, within EX31_RS19005 at 390.507 kb on + strand, within EX31_RS19005 at 390.507 kb on + strand, within EX31_RS19005 at 390.508 kb on - strand, within EX31_RS19005 at 390.518 kb on + strand, within EX31_RS19005 at 390.518 kb on + strand, within EX31_RS19005 at 390.519 kb on - strand, within EX31_RS19005 at 390.671 kb on - strand, within EX31_RS19005 at 390.671 kb on - strand, within EX31_RS19005 at 390.671 kb on - strand, within EX31_RS19005 at 390.671 kb on - strand, within EX31_RS19005 at 390.671 kb on - strand, within EX31_RS19005 at 390.671 kb on - strand, within EX31_RS19005 at 390.735 kb on + strand, within EX31_RS19005 at 390.735 kb on + strand, within EX31_RS19005 at 390.736 kb on - strand, within EX31_RS19005 at 390.736 kb on - strand, within EX31_RS19005 at 390.736 kb on - strand, within EX31_RS19005 at 390.736 kb on - strand, within EX31_RS19005 at 390.736 kb on - strand, within EX31_RS19005 at 390.736 kb on - strand, within EX31_RS19005 at 390.736 kb on - strand, within EX31_RS19005 at 390.769 kb on - strand, within EX31_RS19005 at 390.795 kb on + strand, within EX31_RS19005 at 390.854 kb on - strand, within EX31_RS19005 at 390.911 kb on - strand at 391.005 kb on + strand at 391.005 kb on + strand at 391.006 kb on - strand at 391.007 kb on + strand at 391.007 kb on + strand at 391.007 kb on + strand at 391.007 kb on + strand at 391.007 kb on + strand at 391.007 kb on + strand at 391.007 kb on + strand at 391.008 kb on - strand at 391.008 kb on - strand at 391.008 kb on - strand at 391.008 kb on - strand at 391.008 kb on - strand at 391.008 kb on - strand at 391.008 kb on - strand at 391.008 kb on - strand at 391.008 kb on - strand at 391.008 kb on - strand at 391.076 kb on + strand at 391.126 kb on + strand at 391.126 kb on + strand at 391.126 kb on + strand at 391.139 kb on + strand at 391.139 kb on + strand at 391.139 kb on + strand at 391.140 kb on - strand at 391.140 kb on - strand at 391.140 kb on - strand at 391.140 kb on - strand at 391.422 kb on - strand, within EX31_RS19010 at 391.422 kb on - strand, within EX31_RS19010 at 391.422 kb on - strand, within EX31_RS19010 at 391.484 kb on - strand, within EX31_RS19010 at 391.484 kb on - strand, within EX31_RS19010 at 391.484 kb on - strand, within EX31_RS19010 at 391.484 kb on - strand, within EX31_RS19010 at 391.704 kb on - strand at 391.758 kb on + strand at 391.759 kb on - strand at 391.777 kb on + strand at 391.777 kb on + strand at 391.777 kb on + strand at 391.778 kb on - strand at 391.788 kb on - strand at 391.788 kb on - strand at 391.807 kb on + strand at 391.808 kb on - strand at 391.811 kb on - strand at 391.889 kb on + strand at 391.984 kb on + strand, within EX31_RS19015 at 391.984 kb on + strand, within EX31_RS19015 at 391.984 kb on + strand, within EX31_RS19015 at 391.984 kb on + strand, within EX31_RS19015 at 391.985 kb on - strand, within EX31_RS19015 at 391.985 kb on - strand, within EX31_RS19015 at 391.985 kb on - strand, within EX31_RS19015 at 391.985 kb on - strand, within EX31_RS19015 at 392.001 kb on + strand, within EX31_RS19015 at 392.001 kb on + strand, within EX31_RS19015 at 392.002 kb on - strand, within EX31_RS19015 at 392.055 kb on + strand, within EX31_RS19015
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 388,354 + -0.4 388,354 + +0.5 388,373 + -0.6 388,373 + +0.9 388,373 + +0.2 388,374 - -0.1 388,374 - -0.0 388,374 - -1.7 388,378 - -1.4 388,395 + +0.3 388,396 - -0.7 388,443 + +1.0 388,444 - +0.9 388,464 + +0.3 388,478 + -0.7 388,478 + -0.6 388,478 + +0.6 388,478 + -0.1 388,478 + -0.7 388,478 + +0.4 388,478 + -2.5 388,478 + +1.5 388,478 + +0.9 388,478 + -1.6 388,479 - +1.0 388,479 - +0.9 388,479 - -0.1 388,479 - +1.1 388,487 + +0.9 388,488 - +0.9 388,488 - +1.5 388,488 - -2.2 388,501 + +1.5 388,501 + -0.2 388,502 - +0.6 388,502 - +1.0 388,537 + +0.9 388,538 - -1.7 388,541 - +0.9 388,541 - +0.2 388,541 - +1.7 388,543 + +0.8 388,543 + -0.8 388,544 - +1.1 388,544 - -0.1 388,661 - EX31_RS19000 0.23 -0.2 388,661 - EX31_RS19000 0.23 -2.2 388,661 - EX31_RS19000 0.23 -1.6 388,684 + EX31_RS19000 0.26 -0.4 388,741 - EX31_RS19000 0.35 +0.4 388,938 + EX31_RS19000 0.67 +0.1 388,938 + EX31_RS19000 0.67 -0.1 388,938 + EX31_RS19000 0.67 +1.3 388,938 + EX31_RS19000 0.67 -0.6 388,938 + EX31_RS19000 0.67 -0.1 388,938 + EX31_RS19000 0.67 +0.0 388,939 - EX31_RS19000 0.67 -1.0 388,939 - EX31_RS19000 0.67 +0.4 388,939 - EX31_RS19000 0.67 +0.7 388,975 - EX31_RS19000 0.72 -1.0 389,041 + EX31_RS19000 0.83 -0.1 389,069 + EX31_RS19000 0.87 +0.5 389,095 + +1.2 389,096 - +1.0 389,158 + +1.1 389,158 + +1.4 389,158 + -0.5 389,159 - +1.9 389,206 - +0.2 389,214 + -1.3 389,215 - -0.2 389,215 - -0.3 389,215 - +0.1 389,235 - +1.3 389,235 - +0.0 389,246 + +0.6 389,247 - -0.1 389,247 - +1.0 389,457 - EX31_RS19005 0.12 -1.1 389,497 - EX31_RS19005 0.14 -0.3 389,497 - EX31_RS19005 0.14 +0.6 389,532 - EX31_RS19005 0.16 +0.7 389,577 - EX31_RS19005 0.18 +0.4 389,721 + EX31_RS19005 0.26 +0.7 389,722 - EX31_RS19005 0.26 -1.1 389,807 + EX31_RS19005 0.31 +1.9 389,808 - EX31_RS19005 0.31 -1.1 389,866 - EX31_RS19005 0.34 +0.5 389,866 - EX31_RS19005 0.34 -0.2 389,919 - EX31_RS19005 0.37 -0.9 389,982 - EX31_RS19005 0.40 +1.2 389,982 - EX31_RS19005 0.40 +1.2 390,036 + EX31_RS19005 0.43 -1.0 390,037 - EX31_RS19005 0.43 -1.3 390,037 - EX31_RS19005 0.43 -0.1 390,078 + EX31_RS19005 0.45 +1.5 390,078 + EX31_RS19005 0.45 -0.3 390,079 - EX31_RS19005 0.45 -0.1 390,135 + EX31_RS19005 0.48 -2.7 390,135 + EX31_RS19005 0.48 -0.7 390,136 - EX31_RS19005 0.48 +0.4 390,136 - EX31_RS19005 0.48 +0.3 390,167 + EX31_RS19005 0.50 -0.5 390,193 - EX31_RS19005 0.52 -0.7 390,193 - EX31_RS19005 0.52 +0.4 390,284 - EX31_RS19005 0.56 +0.3 390,317 + EX31_RS19005 0.58 -1.7 390,318 - EX31_RS19005 0.58 +0.3 390,469 + EX31_RS19005 0.66 -1.7 390,498 + EX31_RS19005 0.68 +1.0 390,498 + EX31_RS19005 0.68 +0.1 390,507 + EX31_RS19005 0.68 +1.4 390,507 + EX31_RS19005 0.68 +2.3 390,508 - EX31_RS19005 0.68 -0.5 390,518 + EX31_RS19005 0.69 -0.8 390,518 + EX31_RS19005 0.69 -2.8 390,519 - EX31_RS19005 0.69 -0.3 390,671 - EX31_RS19005 0.77 +0.7 390,671 - EX31_RS19005 0.77 +0.0 390,671 - EX31_RS19005 0.77 +0.9 390,671 - EX31_RS19005 0.77 +0.6 390,671 - EX31_RS19005 0.77 +0.2 390,671 - EX31_RS19005 0.77 +0.3 390,735 + EX31_RS19005 0.81 -1.5 390,735 + EX31_RS19005 0.81 +0.7 390,736 - EX31_RS19005 0.81 +0.5 390,736 - EX31_RS19005 0.81 +0.1 390,736 - EX31_RS19005 0.81 +1.3 390,736 - EX31_RS19005 0.81 +1.9 390,736 - EX31_RS19005 0.81 +0.2 390,736 - EX31_RS19005 0.81 +1.1 390,736 - EX31_RS19005 0.81 -0.4 390,769 - EX31_RS19005 0.82 -0.0 390,795 + EX31_RS19005 0.84 +0.3 390,854 - EX31_RS19005 0.87 +0.1 390,911 - +0.3 391,005 + +1.0 391,005 + -0.1 391,006 - +0.6 391,007 + -0.9 391,007 + -0.6 391,007 + +1.5 391,007 + +1.1 391,007 + -0.1 391,007 + -0.8 391,007 + -0.9 391,008 - -0.6 391,008 - +0.6 391,008 - +1.1 391,008 - -0.5 391,008 - +0.4 391,008 - -0.9 391,008 - +0.8 391,008 - -0.5 391,008 - +0.0 391,008 - -0.1 391,076 + -0.6 391,126 + +0.1 391,126 + +1.1 391,126 + +0.5 391,139 + +1.4 391,139 + +1.5 391,139 + -1.1 391,140 - +0.1 391,140 - +0.9 391,140 - -0.3 391,140 - +0.7 391,422 - EX31_RS19010 0.63 -0.6 391,422 - EX31_RS19010 0.63 -1.4 391,422 - EX31_RS19010 0.63 +0.9 391,484 - EX31_RS19010 0.81 -1.5 391,484 - EX31_RS19010 0.81 +0.5 391,484 - EX31_RS19010 0.81 -1.1 391,484 - EX31_RS19010 0.81 -0.5 391,704 - -0.6 391,758 + -0.8 391,759 - -0.6 391,777 + +0.7 391,777 + +0.0 391,777 + -0.8 391,778 - -1.1 391,788 - -0.2 391,788 - -0.4 391,807 + -0.2 391,808 - +0.7 391,811 - +1.6 391,889 + -0.1 391,984 + EX31_RS19015 0.14 +1.1 391,984 + EX31_RS19015 0.14 +0.8 391,984 + EX31_RS19015 0.14 -0.7 391,984 + EX31_RS19015 0.14 +0.3 391,985 - EX31_RS19015 0.14 -2.0 391,985 - EX31_RS19015 0.14 -1.2 391,985 - EX31_RS19015 0.14 +0.3 391,985 - EX31_RS19015 0.14 -0.1 392,001 + EX31_RS19015 0.16 +0.6 392,001 + EX31_RS19015 0.16 -0.4 392,002 - EX31_RS19015 0.16 +0.8 392,055 + EX31_RS19015 0.21 +1.0
Or see this region's nucleotide sequence