Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12595 and EX31_RS12600 are separated by 23 nucleotides EX31_RS12600 and EX31_RS12605 are separated by 247 nucleotides EX31_RS12605 and EX31_RS12610 are separated by 95 nucleotides
EX31_RS12595: EX31_RS12595 - type II and III secretion system protein, at 190,262 to 191,710
_RS12595
EX31_RS12600: EX31_RS12600 - pilus assembly protein, at 191,734 to 191,985
_RS12600
EX31_RS12605: EX31_RS12605 - type II secretion system protein, at 192,233 to 193,900
_RS12605
EX31_RS12610: EX31_RS12610 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 193,996 to 194,694
_RS12610
Position (kb) 192
193
194 Strain fitness (log2 ratio) -2
-1
0
1
2 at 191.233 kb on - strand, within EX31_RS12595 at 191.233 kb on - strand, within EX31_RS12595 at 191.239 kb on + strand, within EX31_RS12595 at 191.239 kb on + strand, within EX31_RS12595 at 191.240 kb on - strand, within EX31_RS12595 at 191.240 kb on - strand, within EX31_RS12595 at 191.240 kb on - strand, within EX31_RS12595 at 191.241 kb on + strand, within EX31_RS12595 at 191.241 kb on + strand, within EX31_RS12595 at 191.241 kb on + strand, within EX31_RS12595 at 191.242 kb on - strand, within EX31_RS12595 at 191.242 kb on - strand, within EX31_RS12595 at 191.254 kb on + strand, within EX31_RS12595 at 191.261 kb on - strand, within EX31_RS12595 at 191.264 kb on - strand, within EX31_RS12595 at 191.264 kb on - strand, within EX31_RS12595 at 191.442 kb on - strand, within EX31_RS12595 at 191.442 kb on - strand, within EX31_RS12595 at 191.442 kb on - strand, within EX31_RS12595 at 191.442 kb on - strand, within EX31_RS12595 at 191.467 kb on + strand, within EX31_RS12595 at 191.527 kb on - strand, within EX31_RS12595 at 191.531 kb on + strand, within EX31_RS12595 at 191.543 kb on + strand, within EX31_RS12595 at 191.548 kb on - strand, within EX31_RS12595 at 191.568 kb on + strand at 191.610 kb on - strand at 191.610 kb on - strand at 191.748 kb on + strand at 191.818 kb on + strand, within EX31_RS12600 at 191.870 kb on + strand, within EX31_RS12600 at 191.875 kb on + strand, within EX31_RS12600 at 191.875 kb on + strand, within EX31_RS12600 at 191.879 kb on + strand, within EX31_RS12600 at 191.907 kb on + strand, within EX31_RS12600 at 191.907 kb on + strand, within EX31_RS12600 at 191.935 kb on - strand, within EX31_RS12600 at 192.009 kb on + strand at 192.009 kb on + strand at 192.009 kb on + strand at 192.009 kb on + strand at 192.009 kb on + strand at 192.009 kb on + strand at 192.009 kb on + strand at 192.010 kb on - strand at 192.010 kb on - strand at 192.010 kb on - strand at 192.010 kb on - strand at 192.012 kb on + strand at 192.013 kb on - strand at 192.085 kb on + strand at 192.211 kb on + strand at 192.218 kb on + strand at 192.219 kb on - strand at 192.220 kb on + strand at 192.221 kb on - strand at 192.221 kb on - strand at 192.221 kb on - strand at 192.235 kb on - strand at 192.251 kb on + strand at 192.279 kb on - strand at 192.286 kb on + strand at 192.286 kb on + strand at 192.287 kb on - strand at 192.287 kb on - strand at 192.302 kb on + strand at 192.325 kb on + strand at 192.328 kb on + strand at 192.390 kb on + strand at 192.412 kb on + strand, within EX31_RS12605 at 192.416 kb on - strand, within EX31_RS12605 at 192.443 kb on + strand, within EX31_RS12605 at 192.444 kb on - strand, within EX31_RS12605 at 192.452 kb on + strand, within EX31_RS12605 at 192.453 kb on - strand, within EX31_RS12605 at 192.453 kb on - strand, within EX31_RS12605 at 192.455 kb on + strand, within EX31_RS12605 at 192.455 kb on + strand, within EX31_RS12605 at 192.564 kb on - strand, within EX31_RS12605 at 192.565 kb on + strand, within EX31_RS12605 at 192.565 kb on + strand, within EX31_RS12605 at 192.565 kb on + strand, within EX31_RS12605 at 192.565 kb on + strand, within EX31_RS12605 at 192.698 kb on - strand, within EX31_RS12605 at 192.702 kb on + strand, within EX31_RS12605 at 192.702 kb on + strand, within EX31_RS12605 at 192.702 kb on + strand, within EX31_RS12605 at 192.703 kb on - strand, within EX31_RS12605 at 192.703 kb on - strand, within EX31_RS12605 at 192.703 kb on - strand, within EX31_RS12605 at 192.719 kb on - strand, within EX31_RS12605 at 192.769 kb on - strand, within EX31_RS12605 at 192.769 kb on - strand, within EX31_RS12605 at 192.769 kb on - strand, within EX31_RS12605 at 192.791 kb on + strand, within EX31_RS12605 at 192.792 kb on - strand, within EX31_RS12605 at 192.859 kb on + strand, within EX31_RS12605 at 192.859 kb on + strand, within EX31_RS12605 at 192.860 kb on - strand, within EX31_RS12605 at 192.860 kb on - strand, within EX31_RS12605 at 192.860 kb on - strand, within EX31_RS12605 at 192.860 kb on - strand, within EX31_RS12605 at 192.860 kb on - strand, within EX31_RS12605 at 192.965 kb on - strand, within EX31_RS12605 at 192.992 kb on - strand, within EX31_RS12605 at 193.072 kb on + strand, within EX31_RS12605 at 193.084 kb on + strand, within EX31_RS12605 at 193.084 kb on + strand, within EX31_RS12605 at 193.085 kb on - strand, within EX31_RS12605 at 193.095 kb on + strand, within EX31_RS12605 at 193.095 kb on + strand, within EX31_RS12605 at 193.096 kb on - strand, within EX31_RS12605 at 193.120 kb on + strand, within EX31_RS12605 at 193.120 kb on + strand, within EX31_RS12605 at 193.120 kb on + strand, within EX31_RS12605 at 193.120 kb on + strand, within EX31_RS12605 at 193.121 kb on - strand, within EX31_RS12605 at 193.121 kb on - strand, within EX31_RS12605 at 193.144 kb on + strand, within EX31_RS12605 at 193.144 kb on + strand, within EX31_RS12605 at 193.145 kb on - strand, within EX31_RS12605 at 193.145 kb on - strand, within EX31_RS12605 at 193.218 kb on + strand, within EX31_RS12605 at 193.218 kb on + strand, within EX31_RS12605 at 193.219 kb on - strand, within EX31_RS12605 at 193.219 kb on - strand, within EX31_RS12605 at 193.219 kb on - strand, within EX31_RS12605 at 193.246 kb on - strand, within EX31_RS12605 at 193.265 kb on - strand, within EX31_RS12605 at 193.278 kb on + strand, within EX31_RS12605 at 193.279 kb on - strand, within EX31_RS12605 at 193.279 kb on - strand, within EX31_RS12605 at 193.297 kb on + strand, within EX31_RS12605 at 193.318 kb on + strand, within EX31_RS12605 at 193.410 kb on + strand, within EX31_RS12605 at 193.410 kb on + strand, within EX31_RS12605 at 193.410 kb on + strand, within EX31_RS12605 at 193.410 kb on + strand, within EX31_RS12605 at 193.410 kb on + strand, within EX31_RS12605 at 193.411 kb on - strand, within EX31_RS12605 at 193.439 kb on - strand, within EX31_RS12605 at 193.445 kb on + strand, within EX31_RS12605 at 193.446 kb on - strand, within EX31_RS12605 at 193.446 kb on - strand, within EX31_RS12605 at 193.497 kb on + strand, within EX31_RS12605 at 193.497 kb on + strand, within EX31_RS12605 at 193.535 kb on - strand, within EX31_RS12605 at 193.547 kb on - strand, within EX31_RS12605 at 193.547 kb on - strand, within EX31_RS12605 at 193.589 kb on - strand, within EX31_RS12605 at 193.645 kb on + strand, within EX31_RS12605 at 193.757 kb on + strand at 193.757 kb on + strand at 193.758 kb on - strand at 193.758 kb on - strand at 193.870 kb on + strand at 193.871 kb on - strand at 193.985 kb on + strand at 193.993 kb on + strand at 193.994 kb on - strand at 193.997 kb on + strand at 193.997 kb on + strand at 193.998 kb on - strand at 193.998 kb on - strand at 194.040 kb on + strand at 194.041 kb on - strand at 194.115 kb on + strand, within EX31_RS12610 at 194.115 kb on + strand, within EX31_RS12610 at 194.149 kb on + strand, within EX31_RS12610 at 194.187 kb on - strand, within EX31_RS12610 at 194.187 kb on - strand, within EX31_RS12610 at 194.187 kb on - strand, within EX31_RS12610 at 194.217 kb on + strand, within EX31_RS12610 at 194.235 kb on + strand, within EX31_RS12610 at 194.258 kb on + strand, within EX31_RS12610 at 194.259 kb on - strand, within EX31_RS12610 at 194.259 kb on - strand, within EX31_RS12610 at 194.277 kb on + strand, within EX31_RS12610 at 194.278 kb on - strand, within EX31_RS12610 at 194.291 kb on + strand, within EX31_RS12610 at 194.292 kb on - strand, within EX31_RS12610 at 194.292 kb on - strand, within EX31_RS12610 at 194.295 kb on + strand, within EX31_RS12610 at 194.296 kb on - strand, within EX31_RS12610 at 194.326 kb on + strand, within EX31_RS12610 at 194.326 kb on + strand, within EX31_RS12610 at 194.327 kb on - strand, within EX31_RS12610 at 194.327 kb on - strand, within EX31_RS12610 at 194.327 kb on - strand, within EX31_RS12610 at 194.327 kb on - strand, within EX31_RS12610 at 194.328 kb on + strand, within EX31_RS12610 at 194.329 kb on - strand, within EX31_RS12610 at 194.329 kb on - strand, within EX31_RS12610 at 194.329 kb on - strand, within EX31_RS12610 at 194.397 kb on + strand, within EX31_RS12610 at 194.397 kb on + strand, within EX31_RS12610 at 194.398 kb on - strand, within EX31_RS12610 at 194.410 kb on - strand, within EX31_RS12610 at 194.425 kb on - strand, within EX31_RS12610 at 194.425 kb on - strand, within EX31_RS12610 at 194.457 kb on - strand, within EX31_RS12610 at 194.473 kb on - strand, within EX31_RS12610 at 194.474 kb on + strand, within EX31_RS12610 at 194.474 kb on + strand, within EX31_RS12610 at 194.475 kb on - strand, within EX31_RS12610 at 194.475 kb on - strand, within EX31_RS12610 at 194.475 kb on - strand, within EX31_RS12610 at 194.475 kb on - strand, within EX31_RS12610 at 194.506 kb on - strand, within EX31_RS12610 at 194.508 kb on + strand, within EX31_RS12610 at 194.509 kb on - strand, within EX31_RS12610 at 194.509 kb on - strand, within EX31_RS12610 at 194.509 kb on - strand, within EX31_RS12610 at 194.509 kb on - strand, within EX31_RS12610 at 194.566 kb on + strand, within EX31_RS12610 at 194.566 kb on + strand, within EX31_RS12610 at 194.566 kb on + strand, within EX31_RS12610 at 194.567 kb on - strand, within EX31_RS12610 at 194.618 kb on - strand, within EX31_RS12610
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 191,233 - EX31_RS12595 0.67 +0.5 191,233 - EX31_RS12595 0.67 +1.6 191,239 + EX31_RS12595 0.67 +0.9 191,239 + EX31_RS12595 0.67 -0.9 191,240 - EX31_RS12595 0.67 +0.5 191,240 - EX31_RS12595 0.67 +0.4 191,240 - EX31_RS12595 0.67 +0.9 191,241 + EX31_RS12595 0.68 +1.2 191,241 + EX31_RS12595 0.68 +1.1 191,241 + EX31_RS12595 0.68 -0.1 191,242 - EX31_RS12595 0.68 +1.0 191,242 - EX31_RS12595 0.68 +1.0 191,254 + EX31_RS12595 0.68 +0.2 191,261 - EX31_RS12595 0.69 +1.1 191,264 - EX31_RS12595 0.69 -0.7 191,264 - EX31_RS12595 0.69 +1.1 191,442 - EX31_RS12595 0.81 -2.3 191,442 - EX31_RS12595 0.81 +1.0 191,442 - EX31_RS12595 0.81 -0.6 191,442 - EX31_RS12595 0.81 -1.3 191,467 + EX31_RS12595 0.83 +1.3 191,527 - EX31_RS12595 0.87 +0.4 191,531 + EX31_RS12595 0.88 +1.5 191,543 + EX31_RS12595 0.88 -0.5 191,548 - EX31_RS12595 0.89 -0.7 191,568 + -0.1 191,610 - +0.6 191,610 - +0.8 191,748 + +0.8 191,818 + EX31_RS12600 0.33 -0.3 191,870 + EX31_RS12600 0.54 +0.2 191,875 + EX31_RS12600 0.56 +0.6 191,875 + EX31_RS12600 0.56 +0.8 191,879 + EX31_RS12600 0.58 +0.9 191,907 + EX31_RS12600 0.69 -0.2 191,907 + EX31_RS12600 0.69 -1.7 191,935 - EX31_RS12600 0.80 -0.6 192,009 + +0.5 192,009 + +0.8 192,009 + +0.5 192,009 + -0.2 192,009 + -0.2 192,009 + +0.6 192,009 + +0.8 192,010 - -0.2 192,010 - +1.6 192,010 - -0.0 192,010 - +1.2 192,012 + +1.2 192,013 - +0.7 192,085 + -0.2 192,211 + -0.2 192,218 + +0.9 192,219 - +1.0 192,220 + -1.2 192,221 - +0.2 192,221 - +0.3 192,221 - +0.3 192,235 - +1.3 192,251 + -0.7 192,279 - +0.4 192,286 + -0.7 192,286 + +2.0 192,287 - -0.6 192,287 - -0.6 192,302 + +0.4 192,325 + +0.5 192,328 + +0.7 192,390 + -0.2 192,412 + EX31_RS12605 0.11 -0.2 192,416 - EX31_RS12605 0.11 +0.7 192,443 + EX31_RS12605 0.13 -0.5 192,444 - EX31_RS12605 0.13 +1.1 192,452 + EX31_RS12605 0.13 +1.0 192,453 - EX31_RS12605 0.13 +1.2 192,453 - EX31_RS12605 0.13 -0.4 192,455 + EX31_RS12605 0.13 -0.5 192,455 + EX31_RS12605 0.13 +0.2 192,564 - EX31_RS12605 0.20 -0.7 192,565 + EX31_RS12605 0.20 +0.6 192,565 + EX31_RS12605 0.20 +1.3 192,565 + EX31_RS12605 0.20 +1.0 192,565 + EX31_RS12605 0.20 +0.7 192,698 - EX31_RS12605 0.28 +0.5 192,702 + EX31_RS12605 0.28 +0.1 192,702 + EX31_RS12605 0.28 -0.8 192,702 + EX31_RS12605 0.28 +0.1 192,703 - EX31_RS12605 0.28 +0.0 192,703 - EX31_RS12605 0.28 -1.0 192,703 - EX31_RS12605 0.28 +1.3 192,719 - EX31_RS12605 0.29 +1.9 192,769 - EX31_RS12605 0.32 +1.7 192,769 - EX31_RS12605 0.32 -1.5 192,769 - EX31_RS12605 0.32 -0.8 192,791 + EX31_RS12605 0.33 +0.8 192,792 - EX31_RS12605 0.34 -0.2 192,859 + EX31_RS12605 0.38 +0.9 192,859 + EX31_RS12605 0.38 +0.4 192,860 - EX31_RS12605 0.38 +0.2 192,860 - EX31_RS12605 0.38 -0.2 192,860 - EX31_RS12605 0.38 +0.2 192,860 - EX31_RS12605 0.38 +0.9 192,860 - EX31_RS12605 0.38 -0.4 192,965 - EX31_RS12605 0.44 -1.5 192,992 - EX31_RS12605 0.46 +0.6 193,072 + EX31_RS12605 0.50 +1.7 193,084 + EX31_RS12605 0.51 +0.3 193,084 + EX31_RS12605 0.51 -0.7 193,085 - EX31_RS12605 0.51 -0.8 193,095 + EX31_RS12605 0.52 +1.2 193,095 + EX31_RS12605 0.52 -0.0 193,096 - EX31_RS12605 0.52 +0.2 193,120 + EX31_RS12605 0.53 +0.8 193,120 + EX31_RS12605 0.53 +1.5 193,120 + EX31_RS12605 0.53 +0.3 193,120 + EX31_RS12605 0.53 +0.4 193,121 - EX31_RS12605 0.53 +0.1 193,121 - EX31_RS12605 0.53 +0.8 193,144 + EX31_RS12605 0.55 -0.1 193,144 + EX31_RS12605 0.55 +0.1 193,145 - EX31_RS12605 0.55 -0.7 193,145 - EX31_RS12605 0.55 -0.5 193,218 + EX31_RS12605 0.59 +1.1 193,218 + EX31_RS12605 0.59 +0.3 193,219 - EX31_RS12605 0.59 -0.7 193,219 - EX31_RS12605 0.59 +0.8 193,219 - EX31_RS12605 0.59 +0.2 193,246 - EX31_RS12605 0.61 -0.3 193,265 - EX31_RS12605 0.62 +0.9 193,278 + EX31_RS12605 0.63 -1.2 193,279 - EX31_RS12605 0.63 +0.2 193,279 - EX31_RS12605 0.63 +0.3 193,297 + EX31_RS12605 0.64 +0.2 193,318 + EX31_RS12605 0.65 +1.0 193,410 + EX31_RS12605 0.71 -1.2 193,410 + EX31_RS12605 0.71 -0.4 193,410 + EX31_RS12605 0.71 +0.6 193,410 + EX31_RS12605 0.71 -0.3 193,410 + EX31_RS12605 0.71 +0.1 193,411 - EX31_RS12605 0.71 -0.4 193,439 - EX31_RS12605 0.72 +0.1 193,445 + EX31_RS12605 0.73 +0.3 193,446 - EX31_RS12605 0.73 +0.1 193,446 - EX31_RS12605 0.73 -0.8 193,497 + EX31_RS12605 0.76 +2.0 193,497 + EX31_RS12605 0.76 +2.5 193,535 - EX31_RS12605 0.78 +0.1 193,547 - EX31_RS12605 0.79 +0.5 193,547 - EX31_RS12605 0.79 +0.8 193,589 - EX31_RS12605 0.81 +0.5 193,645 + EX31_RS12605 0.85 +0.2 193,757 + -0.6 193,757 + +0.2 193,758 - +0.7 193,758 - +0.7 193,870 + +0.3 193,871 - +1.2 193,985 + -1.3 193,993 + -0.9 193,994 - -0.1 193,997 + +1.2 193,997 + +0.2 193,998 - -1.6 193,998 - +0.5 194,040 + -0.8 194,041 - +0.1 194,115 + EX31_RS12610 0.17 -0.6 194,115 + EX31_RS12610 0.17 +1.0 194,149 + EX31_RS12610 0.22 +0.1 194,187 - EX31_RS12610 0.27 +0.3 194,187 - EX31_RS12610 0.27 -0.7 194,187 - EX31_RS12610 0.27 +0.4 194,217 + EX31_RS12610 0.32 +1.1 194,235 + EX31_RS12610 0.34 +1.1 194,258 + EX31_RS12610 0.37 +0.6 194,259 - EX31_RS12610 0.38 -0.4 194,259 - EX31_RS12610 0.38 +0.3 194,277 + EX31_RS12610 0.40 +0.5 194,278 - EX31_RS12610 0.40 +1.8 194,291 + EX31_RS12610 0.42 +1.1 194,292 - EX31_RS12610 0.42 -0.0 194,292 - EX31_RS12610 0.42 +1.0 194,295 + EX31_RS12610 0.43 -1.1 194,296 - EX31_RS12610 0.43 +1.9 194,326 + EX31_RS12610 0.47 +0.2 194,326 + EX31_RS12610 0.47 +0.2 194,327 - EX31_RS12610 0.47 +0.1 194,327 - EX31_RS12610 0.47 -0.6 194,327 - EX31_RS12610 0.47 -0.8 194,327 - EX31_RS12610 0.47 +0.5 194,328 + EX31_RS12610 0.47 +0.5 194,329 - EX31_RS12610 0.48 -0.6 194,329 - EX31_RS12610 0.48 +0.5 194,329 - EX31_RS12610 0.48 +0.1 194,397 + EX31_RS12610 0.57 +0.9 194,397 + EX31_RS12610 0.57 +0.8 194,398 - EX31_RS12610 0.58 +0.8 194,410 - EX31_RS12610 0.59 +0.4 194,425 - EX31_RS12610 0.61 -0.5 194,425 - EX31_RS12610 0.61 +0.5 194,457 - EX31_RS12610 0.66 -0.3 194,473 - EX31_RS12610 0.68 -1.0 194,474 + EX31_RS12610 0.68 -0.7 194,474 + EX31_RS12610 0.68 -0.2 194,475 - EX31_RS12610 0.69 +2.0 194,475 - EX31_RS12610 0.69 +0.2 194,475 - EX31_RS12610 0.69 +0.5 194,475 - EX31_RS12610 0.69 -2.4 194,506 - EX31_RS12610 0.73 +1.1 194,508 + EX31_RS12610 0.73 +1.6 194,509 - EX31_RS12610 0.73 +0.0 194,509 - EX31_RS12610 0.73 +0.1 194,509 - EX31_RS12610 0.73 +2.0 194,509 - EX31_RS12610 0.73 +0.0 194,566 + EX31_RS12610 0.82 +0.3 194,566 + EX31_RS12610 0.82 +0.3 194,566 + EX31_RS12610 0.82 +2.1 194,567 - EX31_RS12610 0.82 +1.8 194,618 - EX31_RS12610 0.89 -0.4
Or see this region's nucleotide sequence