Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11250 and EX31_RS11255 are separated by 389 nucleotides EX31_RS11255 and EX31_RS11260 overlap by 8 nucleotides EX31_RS11260 and EX31_RS11265 are separated by 304 nucleotides
EX31_RS11250: EX31_RS11250 - amino acid ABC transporter ATP-binding protein, at 182,873 to 183,622
_RS11250
EX31_RS11255: EX31_RS11255 - CidA/LrgA family protein, at 184,012 to 184,416
_RS11255
EX31_RS11260: EX31_RS11260 - CidB/LrgB family autolysis modulator, at 184,409 to 185,104
_RS11260
EX31_RS11265: EX31_RS11265 - flagellar hook-filament junction protein FlgL, at 185,409 to 186,371
_RS11265
Position (kb) 184
185 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 183.040 kb on + strand, within EX31_RS11250 at 183.059 kb on + strand, within EX31_RS11250 at 183.059 kb on + strand, within EX31_RS11250 at 183.059 kb on + strand, within EX31_RS11250 at 183.060 kb on - strand, within EX31_RS11250 at 183.060 kb on - strand, within EX31_RS11250 at 183.140 kb on + strand, within EX31_RS11250 at 183.162 kb on + strand, within EX31_RS11250 at 183.365 kb on - strand, within EX31_RS11250 at 183.436 kb on + strand, within EX31_RS11250 at 183.436 kb on + strand, within EX31_RS11250 at 183.436 kb on + strand, within EX31_RS11250 at 183.436 kb on + strand, within EX31_RS11250 at 183.523 kb on + strand, within EX31_RS11250 at 183.621 kb on - strand at 183.697 kb on + strand at 183.810 kb on - strand at 183.934 kb on - strand at 183.934 kb on - strand at 183.971 kb on + strand at 183.972 kb on - strand at 183.978 kb on - strand at 183.986 kb on + strand at 183.987 kb on - strand at 184.006 kb on + strand at 184.007 kb on - strand at 184.043 kb on - strand at 184.066 kb on + strand, within EX31_RS11255 at 184.106 kb on + strand, within EX31_RS11255 at 184.203 kb on + strand, within EX31_RS11255 at 184.203 kb on + strand, within EX31_RS11255 at 184.204 kb on - strand, within EX31_RS11255 at 184.204 kb on - strand, within EX31_RS11255 at 184.219 kb on + strand, within EX31_RS11255 at 184.219 kb on + strand, within EX31_RS11255 at 184.219 kb on + strand, within EX31_RS11255 at 184.219 kb on + strand, within EX31_RS11255 at 184.220 kb on - strand, within EX31_RS11255 at 184.221 kb on + strand, within EX31_RS11255 at 184.264 kb on + strand, within EX31_RS11255 at 184.264 kb on + strand, within EX31_RS11255 at 184.264 kb on + strand, within EX31_RS11255 at 184.268 kb on - strand, within EX31_RS11255 at 184.320 kb on + strand, within EX31_RS11255 at 184.331 kb on + strand, within EX31_RS11255 at 184.331 kb on + strand, within EX31_RS11255 at 184.331 kb on + strand, within EX31_RS11255 at 184.332 kb on - strand, within EX31_RS11255 at 184.348 kb on + strand, within EX31_RS11255 at 184.348 kb on + strand, within EX31_RS11255 at 184.360 kb on + strand, within EX31_RS11255 at 184.360 kb on + strand, within EX31_RS11255 at 184.360 kb on + strand, within EX31_RS11255 at 184.360 kb on + strand, within EX31_RS11255 at 184.360 kb on + strand, within EX31_RS11255 at 184.362 kb on + strand, within EX31_RS11255 at 184.362 kb on + strand, within EX31_RS11255 at 184.362 kb on + strand, within EX31_RS11255 at 184.362 kb on + strand, within EX31_RS11255 at 184.363 kb on - strand, within EX31_RS11255 at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.418 kb on - strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.420 kb on - strand at 184.420 kb on - strand at 184.420 kb on - strand at 184.477 kb on + strand at 184.477 kb on + strand at 184.637 kb on + strand, within EX31_RS11260 at 184.637 kb on + strand, within EX31_RS11260 at 184.637 kb on + strand, within EX31_RS11260 at 184.637 kb on + strand, within EX31_RS11260 at 184.638 kb on - strand, within EX31_RS11260 at 184.638 kb on - strand, within EX31_RS11260 at 184.684 kb on + strand, within EX31_RS11260 at 184.737 kb on + strand, within EX31_RS11260 at 184.738 kb on - strand, within EX31_RS11260 at 184.773 kb on + strand, within EX31_RS11260 at 184.831 kb on + strand, within EX31_RS11260 at 184.832 kb on - strand, within EX31_RS11260 at 184.851 kb on + strand, within EX31_RS11260 at 184.852 kb on - strand, within EX31_RS11260 at 184.852 kb on - strand, within EX31_RS11260 at 184.852 kb on - strand, within EX31_RS11260 at 184.852 kb on - strand, within EX31_RS11260 at 184.852 kb on - strand, within EX31_RS11260 at 185.144 kb on + strand at 185.144 kb on + strand at 185.144 kb on + strand at 185.144 kb on + strand at 185.179 kb on + strand at 185.179 kb on + strand at 185.179 kb on + strand at 185.179 kb on + strand at 185.179 kb on + strand at 185.180 kb on - strand at 185.184 kb on + strand at 185.184 kb on + strand at 185.184 kb on + strand at 185.184 kb on + strand at 185.185 kb on - strand at 185.185 kb on - strand at 185.188 kb on + strand at 185.188 kb on + strand at 185.188 kb on + strand at 185.188 kb on + strand at 185.188 kb on + strand at 185.189 kb on - strand at 185.189 kb on - strand at 185.189 kb on - strand at 185.189 kb on - strand at 185.254 kb on + strand at 185.254 kb on + strand at 185.255 kb on - strand at 185.255 kb on - strand at 185.263 kb on + strand at 185.263 kb on + strand at 185.264 kb on - strand at 185.284 kb on + strand at 185.284 kb on + strand at 185.284 kb on + strand at 185.284 kb on + strand at 185.285 kb on - strand at 185.285 kb on - strand at 185.285 kb on - strand at 185.307 kb on + strand at 185.312 kb on - strand at 185.394 kb on - strand at 185.394 kb on - strand at 185.407 kb on + strand at 185.408 kb on - strand at 185.408 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 183,040 + EX31_RS11250 0.22 +0.9 183,059 + EX31_RS11250 0.25 +0.2 183,059 + EX31_RS11250 0.25 -0.6 183,059 + EX31_RS11250 0.25 +3.0 183,060 - EX31_RS11250 0.25 +0.5 183,060 - EX31_RS11250 0.25 +1.6 183,140 + EX31_RS11250 0.36 -0.2 183,162 + EX31_RS11250 0.39 +1.0 183,365 - EX31_RS11250 0.66 -0.1 183,436 + EX31_RS11250 0.75 +0.1 183,436 + EX31_RS11250 0.75 +0.3 183,436 + EX31_RS11250 0.75 -1.5 183,436 + EX31_RS11250 0.75 +0.9 183,523 + EX31_RS11250 0.87 -0.0 183,621 - +0.3 183,697 + -0.0 183,810 - +1.3 183,934 - -0.8 183,934 - +0.5 183,971 + +3.2 183,972 - +1.8 183,978 - +0.8 183,986 + +0.9 183,987 - +0.2 184,006 + -0.5 184,007 - -0.2 184,043 - +0.1 184,066 + EX31_RS11255 0.13 +0.4 184,106 + EX31_RS11255 0.23 +1.4 184,203 + EX31_RS11255 0.47 +0.8 184,203 + EX31_RS11255 0.47 -2.2 184,204 - EX31_RS11255 0.47 +2.1 184,204 - EX31_RS11255 0.47 +0.2 184,219 + EX31_RS11255 0.51 -0.7 184,219 + EX31_RS11255 0.51 -2.7 184,219 + EX31_RS11255 0.51 -0.7 184,219 + EX31_RS11255 0.51 -0.6 184,220 - EX31_RS11255 0.51 +0.7 184,221 + EX31_RS11255 0.52 -1.2 184,264 + EX31_RS11255 0.62 +1.1 184,264 + EX31_RS11255 0.62 +0.2 184,264 + EX31_RS11255 0.62 -0.4 184,268 - EX31_RS11255 0.63 +0.2 184,320 + EX31_RS11255 0.76 -2.9 184,331 + EX31_RS11255 0.79 +0.2 184,331 + EX31_RS11255 0.79 -0.5 184,331 + EX31_RS11255 0.79 +0.2 184,332 - EX31_RS11255 0.79 +0.3 184,348 + EX31_RS11255 0.83 +0.3 184,348 + EX31_RS11255 0.83 -0.9 184,360 + EX31_RS11255 0.86 +0.4 184,360 + EX31_RS11255 0.86 +0.3 184,360 + EX31_RS11255 0.86 +1.4 184,360 + EX31_RS11255 0.86 +1.5 184,360 + EX31_RS11255 0.86 +0.7 184,362 + EX31_RS11255 0.86 +0.2 184,362 + EX31_RS11255 0.86 +1.3 184,362 + EX31_RS11255 0.86 +0.4 184,362 + EX31_RS11255 0.86 +0.6 184,363 - EX31_RS11255 0.87 +0.3 184,417 + +0.3 184,417 + -1.2 184,417 + +1.2 184,417 + -0.0 184,417 + -1.2 184,417 + -0.5 184,417 + +0.6 184,417 + -0.2 184,417 + -0.7 184,418 - +0.2 184,419 + -0.5 184,419 + -0.0 184,419 + +1.4 184,419 + +3.0 184,419 + +0.1 184,419 + -0.9 184,419 + -0.5 184,419 + -1.2 184,420 - +1.6 184,420 - +2.0 184,420 - +0.3 184,477 + -0.1 184,477 + -0.3 184,637 + EX31_RS11260 0.33 +0.6 184,637 + EX31_RS11260 0.33 +1.6 184,637 + EX31_RS11260 0.33 -1.5 184,637 + EX31_RS11260 0.33 +0.5 184,638 - EX31_RS11260 0.33 +1.1 184,638 - EX31_RS11260 0.33 +1.1 184,684 + EX31_RS11260 0.40 +0.1 184,737 + EX31_RS11260 0.47 +0.1 184,738 - EX31_RS11260 0.47 -1.1 184,773 + EX31_RS11260 0.52 +0.6 184,831 + EX31_RS11260 0.61 +0.4 184,832 - EX31_RS11260 0.61 +0.6 184,851 + EX31_RS11260 0.64 -0.4 184,852 - EX31_RS11260 0.64 +0.3 184,852 - EX31_RS11260 0.64 -0.0 184,852 - EX31_RS11260 0.64 -0.8 184,852 - EX31_RS11260 0.64 -0.2 184,852 - EX31_RS11260 0.64 +2.6 185,144 + -0.1 185,144 + +0.4 185,144 + -0.7 185,144 + -0.4 185,179 + +0.6 185,179 + +2.1 185,179 + +1.2 185,179 + +0.3 185,179 + +0.4 185,180 - +0.2 185,184 + -1.3 185,184 + -1.4 185,184 + +1.6 185,184 + +0.3 185,185 - -1.4 185,185 - +0.9 185,188 + +1.1 185,188 + +0.3 185,188 + +0.4 185,188 + -0.9 185,188 + -0.5 185,189 - -0.8 185,189 - -0.5 185,189 - -1.1 185,189 - -1.1 185,254 + -0.2 185,254 + +0.3 185,255 - +1.0 185,255 - -0.1 185,263 + +1.2 185,263 + -0.4 185,264 - +1.6 185,284 + +0.6 185,284 + -0.9 185,284 + -0.8 185,284 + -0.9 185,285 - -0.7 185,285 - -0.2 185,285 - +1.4 185,307 + +0.5 185,312 - +0.6 185,394 - -0.5 185,394 - -0.5 185,407 + +0.4 185,408 - +1.0 185,408 - -0.7
Or see this region's nucleotide sequence