Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10190 and EX31_RS10195 are separated by 361 nucleotides EX31_RS10195 and EX31_RS10200 are separated by 150 nucleotides EX31_RS10200 and EX31_RS10205 overlap by 4 nucleotides
EX31_RS10190: EX31_RS10190 - glycosyltransferase, at 250,526 to 251,638
_RS10190
EX31_RS10195: EX31_RS10195 - LysR family transcriptional regulator, at 252,000 to 253,229
_RS10195
EX31_RS10200: EX31_RS10200 - 4-oxalmesaconate hydratase, at 253,380 to 254,126
_RS10200
EX31_RS10205: EX31_RS10205 - 4-carboxy-4-hydroxy-2-oxoadipate aldolase/oxaloacetate decarboxylase, at 254,123 to 254,848
_RS10205
Position (kb) 251
252
253
254 Strain fitness (log2 ratio) -2
-1
0
1
2 at 251.217 kb on + strand, within EX31_RS10190 at 251.535 kb on - strand at 251.702 kb on + strand at 251.703 kb on - strand at 251.703 kb on - strand at 251.706 kb on + strand at 251.803 kb on - strand at 251.914 kb on - strand at 251.914 kb on - strand at 251.917 kb on - strand at 251.925 kb on + strand at 251.925 kb on + strand at 251.950 kb on - strand at 251.964 kb on + strand at 251.988 kb on + strand at 252.449 kb on - strand, within EX31_RS10195 at 252.449 kb on - strand, within EX31_RS10195 at 252.476 kb on + strand, within EX31_RS10195 at 252.689 kb on + strand, within EX31_RS10195 at 252.792 kb on + strand, within EX31_RS10195 at 252.793 kb on - strand, within EX31_RS10195 at 252.855 kb on - strand, within EX31_RS10195 at 252.855 kb on - strand, within EX31_RS10195 at 252.855 kb on - strand, within EX31_RS10195 at 252.855 kb on - strand, within EX31_RS10195 at 252.879 kb on - strand, within EX31_RS10195 at 252.902 kb on + strand, within EX31_RS10195 at 252.903 kb on - strand, within EX31_RS10195 at 252.911 kb on - strand, within EX31_RS10195 at 252.911 kb on - strand, within EX31_RS10195 at 252.955 kb on - strand, within EX31_RS10195 at 253.041 kb on + strand, within EX31_RS10195 at 253.406 kb on + strand at 253.406 kb on + strand at 253.407 kb on - strand at 253.417 kb on + strand at 253.479 kb on - strand, within EX31_RS10200 at 253.479 kb on - strand, within EX31_RS10200 at 253.479 kb on - strand, within EX31_RS10200 at 253.562 kb on + strand, within EX31_RS10200 at 253.563 kb on - strand, within EX31_RS10200 at 253.717 kb on + strand, within EX31_RS10200 at 253.758 kb on + strand, within EX31_RS10200 at 253.764 kb on + strand, within EX31_RS10200 at 253.764 kb on + strand, within EX31_RS10200 at 253.765 kb on - strand, within EX31_RS10200 at 253.765 kb on - strand, within EX31_RS10200 at 253.809 kb on - strand, within EX31_RS10200 at 253.827 kb on + strand, within EX31_RS10200 at 253.827 kb on + strand, within EX31_RS10200 at 253.828 kb on - strand, within EX31_RS10200 at 253.828 kb on - strand, within EX31_RS10200 at 253.829 kb on + strand, within EX31_RS10200 at 253.829 kb on + strand, within EX31_RS10200 at 253.830 kb on - strand, within EX31_RS10200 at 253.995 kb on + strand, within EX31_RS10200 at 253.996 kb on - strand, within EX31_RS10200 at 253.998 kb on + strand, within EX31_RS10200 at 253.998 kb on + strand, within EX31_RS10200 at 253.999 kb on - strand, within EX31_RS10200 at 254.039 kb on + strand, within EX31_RS10200 at 254.039 kb on + strand, within EX31_RS10200 at 254.119 kb on + strand at 254.120 kb on - strand at 254.120 kb on - strand at 254.161 kb on + strand at 254.177 kb on - strand at 254.220 kb on + strand, within EX31_RS10205 at 254.221 kb on - strand, within EX31_RS10205
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 251,217 + EX31_RS10190 0.62 -0.7 251,535 - -2.3 251,702 + +0.5 251,703 - +0.4 251,703 - -0.4 251,706 + -0.1 251,803 - -2.2 251,914 - +0.4 251,914 - +2.0 251,917 - +0.2 251,925 + -0.4 251,925 + +2.5 251,950 - +0.2 251,964 + +1.5 251,988 + +1.5 252,449 - EX31_RS10195 0.37 -1.0 252,449 - EX31_RS10195 0.37 -1.8 252,476 + EX31_RS10195 0.39 -0.9 252,689 + EX31_RS10195 0.56 +0.8 252,792 + EX31_RS10195 0.64 -0.4 252,793 - EX31_RS10195 0.64 -0.4 252,855 - EX31_RS10195 0.70 -1.6 252,855 - EX31_RS10195 0.70 +1.5 252,855 - EX31_RS10195 0.70 -1.2 252,855 - EX31_RS10195 0.70 -0.5 252,879 - EX31_RS10195 0.71 -0.5 252,902 + EX31_RS10195 0.73 -2.3 252,903 - EX31_RS10195 0.73 -1.9 252,911 - EX31_RS10195 0.74 +0.1 252,911 - EX31_RS10195 0.74 -0.5 252,955 - EX31_RS10195 0.78 -1.6 253,041 + EX31_RS10195 0.85 +0.4 253,406 + -0.8 253,406 + -0.6 253,407 - +0.9 253,417 + +0.4 253,479 - EX31_RS10200 0.13 +0.0 253,479 - EX31_RS10200 0.13 +0.0 253,479 - EX31_RS10200 0.13 +1.7 253,562 + EX31_RS10200 0.24 +0.5 253,563 - EX31_RS10200 0.24 +0.0 253,717 + EX31_RS10200 0.45 +1.7 253,758 + EX31_RS10200 0.51 +1.8 253,764 + EX31_RS10200 0.51 +1.5 253,764 + EX31_RS10200 0.51 +0.4 253,765 - EX31_RS10200 0.52 +0.3 253,765 - EX31_RS10200 0.52 -1.2 253,809 - EX31_RS10200 0.57 -0.2 253,827 + EX31_RS10200 0.60 +1.4 253,827 + EX31_RS10200 0.60 -0.8 253,828 - EX31_RS10200 0.60 +0.0 253,828 - EX31_RS10200 0.60 -0.1 253,829 + EX31_RS10200 0.60 -0.5 253,829 + EX31_RS10200 0.60 -0.1 253,830 - EX31_RS10200 0.60 +0.9 253,995 + EX31_RS10200 0.82 -0.3 253,996 - EX31_RS10200 0.82 -0.4 253,998 + EX31_RS10200 0.83 +0.4 253,998 + EX31_RS10200 0.83 -1.1 253,999 - EX31_RS10200 0.83 -0.7 254,039 + EX31_RS10200 0.88 -0.4 254,039 + EX31_RS10200 0.88 +1.2 254,119 + -0.9 254,120 - +0.5 254,120 - -1.1 254,161 + -0.5 254,177 - -0.1 254,220 + EX31_RS10205 0.13 -1.0 254,221 - EX31_RS10205 0.13 +0.7
Or see this region's nucleotide sequence