Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06435 and EX31_RS06440 are separated by 50 nucleotides EX31_RS06440 and EX31_RS06445 are separated by 202 nucleotides EX31_RS06445 and EX31_RS06450 are separated by 65 nucleotides
EX31_RS06435: EX31_RS06435 - DUF333 domain-containing protein, at 57,510 to 57,791
_RS06435
EX31_RS06440: EX31_RS06440 - heat shock protein HslJ, at 57,842 to 58,264
_RS06440
EX31_RS06445: EX31_RS06445 - ABC transporter ATP-binding protein, at 58,467 to 59,570
_RS06445
EX31_RS06450: EX31_RS06450 - ABC transporter substrate-binding protein, at 59,636 to 60,916
_RS06450
Position (kb) 58
59
60 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 57.492 kb on + strand at 57.532 kb on + strand at 57.654 kb on + strand, within EX31_RS06435 at 57.668 kb on + strand, within EX31_RS06435 at 57.668 kb on + strand, within EX31_RS06435 at 57.668 kb on + strand, within EX31_RS06435 at 57.668 kb on + strand, within EX31_RS06435 at 57.668 kb on + strand, within EX31_RS06435 at 57.685 kb on + strand, within EX31_RS06435 at 57.722 kb on + strand, within EX31_RS06435 at 57.723 kb on - strand, within EX31_RS06435 at 57.782 kb on + strand at 57.793 kb on - strand at 57.843 kb on + strand at 57.880 kb on + strand at 57.881 kb on - strand at 57.932 kb on - strand, within EX31_RS06440 at 57.935 kb on - strand, within EX31_RS06440 at 57.935 kb on - strand, within EX31_RS06440 at 57.935 kb on - strand, within EX31_RS06440 at 57.964 kb on + strand, within EX31_RS06440 at 57.965 kb on - strand, within EX31_RS06440 at 57.965 kb on - strand, within EX31_RS06440 at 57.965 kb on - strand, within EX31_RS06440 at 57.965 kb on - strand, within EX31_RS06440 at 57.965 kb on - strand, within EX31_RS06440 at 58.058 kb on - strand, within EX31_RS06440 at 58.058 kb on - strand, within EX31_RS06440 at 58.061 kb on - strand, within EX31_RS06440 at 58.282 kb on - strand at 58.309 kb on + strand at 58.309 kb on + strand at 58.309 kb on + strand at 58.310 kb on - strand at 58.310 kb on - strand at 58.310 kb on - strand at 58.323 kb on - strand at 58.323 kb on - strand at 58.323 kb on - strand at 58.323 kb on - strand at 58.323 kb on - strand at 58.323 kb on - strand at 58.345 kb on - strand at 58.346 kb on + strand at 58.347 kb on - strand at 58.405 kb on + strand at 58.406 kb on - strand at 58.428 kb on - strand at 58.458 kb on + strand at 58.458 kb on + strand at 58.458 kb on + strand at 58.458 kb on + strand at 58.458 kb on + strand at 58.458 kb on + strand at 58.458 kb on + strand at 58.458 kb on + strand at 58.459 kb on - strand at 58.459 kb on - strand at 58.459 kb on - strand at 58.459 kb on - strand at 58.459 kb on - strand at 58.459 kb on - strand at 58.459 kb on - strand at 58.459 kb on - strand at 58.473 kb on + strand at 58.474 kb on - strand at 58.474 kb on - strand at 58.474 kb on - strand at 58.477 kb on + strand at 58.477 kb on + strand at 58.478 kb on - strand at 58.478 kb on - strand at 58.478 kb on - strand at 58.600 kb on + strand, within EX31_RS06445 at 58.600 kb on + strand, within EX31_RS06445 at 58.603 kb on + strand, within EX31_RS06445 at 58.603 kb on + strand, within EX31_RS06445 at 58.603 kb on + strand, within EX31_RS06445 at 58.604 kb on - strand, within EX31_RS06445 at 58.604 kb on - strand, within EX31_RS06445 at 58.641 kb on - strand, within EX31_RS06445 at 58.647 kb on + strand, within EX31_RS06445 at 58.691 kb on + strand, within EX31_RS06445 at 58.692 kb on - strand, within EX31_RS06445 at 59.027 kb on - strand, within EX31_RS06445 at 59.027 kb on - strand, within EX31_RS06445 at 59.031 kb on + strand, within EX31_RS06445 at 59.031 kb on + strand, within EX31_RS06445 at 59.031 kb on + strand, within EX31_RS06445 at 59.031 kb on + strand, within EX31_RS06445 at 59.031 kb on + strand, within EX31_RS06445 at 59.031 kb on + strand, within EX31_RS06445 at 59.031 kb on + strand, within EX31_RS06445 at 59.032 kb on - strand, within EX31_RS06445 at 59.032 kb on - strand, within EX31_RS06445 at 59.032 kb on - strand, within EX31_RS06445 at 59.032 kb on - strand, within EX31_RS06445 at 59.130 kb on + strand, within EX31_RS06445 at 59.130 kb on + strand, within EX31_RS06445 at 59.130 kb on + strand, within EX31_RS06445 at 59.131 kb on - strand, within EX31_RS06445 at 59.132 kb on + strand, within EX31_RS06445 at 59.132 kb on + strand, within EX31_RS06445 at 59.132 kb on + strand, within EX31_RS06445 at 59.132 kb on + strand, within EX31_RS06445 at 59.133 kb on - strand, within EX31_RS06445 at 59.133 kb on - strand, within EX31_RS06445 at 59.133 kb on - strand, within EX31_RS06445 at 59.221 kb on - strand, within EX31_RS06445 at 59.221 kb on - strand, within EX31_RS06445 at 59.221 kb on - strand, within EX31_RS06445 at 59.221 kb on - strand, within EX31_RS06445 at 59.221 kb on - strand, within EX31_RS06445 at 59.232 kb on - strand, within EX31_RS06445 at 59.297 kb on + strand, within EX31_RS06445 at 59.355 kb on + strand, within EX31_RS06445 at 59.355 kb on + strand, within EX31_RS06445 at 59.355 kb on + strand, within EX31_RS06445 at 59.355 kb on + strand, within EX31_RS06445 at 59.355 kb on + strand, within EX31_RS06445 at 59.355 kb on + strand, within EX31_RS06445 at 59.355 kb on + strand, within EX31_RS06445 at 59.355 kb on + strand, within EX31_RS06445 at 59.355 kb on + strand, within EX31_RS06445 at 59.355 kb on + strand, within EX31_RS06445 at 59.356 kb on - strand, within EX31_RS06445 at 59.356 kb on - strand, within EX31_RS06445 at 59.356 kb on - strand, within EX31_RS06445 at 59.356 kb on - strand, within EX31_RS06445 at 59.356 kb on - strand, within EX31_RS06445 at 59.356 kb on - strand, within EX31_RS06445 at 59.356 kb on - strand, within EX31_RS06445 at 59.356 kb on - strand, within EX31_RS06445 at 59.407 kb on + strand, within EX31_RS06445 at 59.407 kb on + strand, within EX31_RS06445 at 59.408 kb on - strand, within EX31_RS06445 at 59.408 kb on - strand, within EX31_RS06445 at 59.498 kb on + strand at 59.499 kb on - strand at 59.568 kb on + strand at 59.568 kb on + strand at 59.568 kb on + strand at 59.568 kb on + strand at 59.568 kb on + strand at 59.569 kb on - strand at 59.569 kb on - strand at 59.569 kb on - strand at 59.569 kb on - strand at 59.598 kb on + strand at 59.599 kb on - strand at 59.608 kb on + strand at 59.608 kb on + strand at 59.609 kb on - strand at 59.609 kb on - strand at 59.636 kb on - strand at 59.724 kb on + strand at 59.732 kb on + strand at 59.732 kb on + strand at 59.733 kb on - strand at 59.733 kb on - strand at 59.733 kb on - strand at 59.733 kb on - strand at 59.768 kb on - strand, within EX31_RS06450 at 59.768 kb on - strand, within EX31_RS06450 at 59.768 kb on - strand, within EX31_RS06450 at 59.776 kb on + strand, within EX31_RS06450 at 59.812 kb on + strand, within EX31_RS06450 at 59.831 kb on + strand, within EX31_RS06450 at 59.843 kb on + strand, within EX31_RS06450 at 60.084 kb on - strand, within EX31_RS06450 at 60.084 kb on - strand, within EX31_RS06450
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 57,492 + -2.4 57,532 + +0.0 57,654 + EX31_RS06435 0.51 -1.3 57,668 + EX31_RS06435 0.56 +0.2 57,668 + EX31_RS06435 0.56 -1.4 57,668 + EX31_RS06435 0.56 -0.3 57,668 + EX31_RS06435 0.56 -0.9 57,668 + EX31_RS06435 0.56 -0.6 57,685 + EX31_RS06435 0.62 +0.5 57,722 + EX31_RS06435 0.75 -0.8 57,723 - EX31_RS06435 0.76 -1.0 57,782 + +0.5 57,793 - +0.6 57,843 + -0.6 57,880 + -2.5 57,881 - -0.3 57,932 - EX31_RS06440 0.21 +2.0 57,935 - EX31_RS06440 0.22 -1.4 57,935 - EX31_RS06440 0.22 +0.2 57,935 - EX31_RS06440 0.22 -0.4 57,964 + EX31_RS06440 0.29 +0.1 57,965 - EX31_RS06440 0.29 -0.8 57,965 - EX31_RS06440 0.29 +1.1 57,965 - EX31_RS06440 0.29 +0.0 57,965 - EX31_RS06440 0.29 +0.6 57,965 - EX31_RS06440 0.29 -0.2 58,058 - EX31_RS06440 0.51 +0.6 58,058 - EX31_RS06440 0.51 +0.1 58,061 - EX31_RS06440 0.52 +0.0 58,282 - -1.1 58,309 + -0.4 58,309 + +1.4 58,309 + -0.2 58,310 - -0.4 58,310 - -1.4 58,310 - +0.2 58,323 - +0.1 58,323 - -0.5 58,323 - +0.3 58,323 - +0.8 58,323 - +0.6 58,323 - +0.6 58,345 - +1.7 58,346 + +0.2 58,347 - +1.4 58,405 + +0.4 58,406 - +3.0 58,428 - +0.6 58,458 + +1.4 58,458 + -1.6 58,458 + -2.2 58,458 + -1.6 58,458 + -0.2 58,458 + -0.5 58,458 + +1.0 58,458 + +0.8 58,459 - -0.4 58,459 - -0.3 58,459 - -0.4 58,459 - +1.5 58,459 - +0.5 58,459 - +0.9 58,459 - +0.0 58,459 - +0.2 58,473 + -0.6 58,474 - +0.0 58,474 - +0.4 58,474 - +1.2 58,477 + -0.2 58,477 + -0.0 58,478 - -1.3 58,478 - -1.0 58,478 - -2.1 58,600 + EX31_RS06445 0.12 -0.0 58,600 + EX31_RS06445 0.12 +0.9 58,603 + EX31_RS06445 0.12 +1.4 58,603 + EX31_RS06445 0.12 -0.5 58,603 + EX31_RS06445 0.12 -1.2 58,604 - EX31_RS06445 0.12 +0.3 58,604 - EX31_RS06445 0.12 -2.5 58,641 - EX31_RS06445 0.16 +1.7 58,647 + EX31_RS06445 0.16 +0.5 58,691 + EX31_RS06445 0.20 -1.4 58,692 - EX31_RS06445 0.20 +0.4 59,027 - EX31_RS06445 0.51 +0.6 59,027 - EX31_RS06445 0.51 +1.4 59,031 + EX31_RS06445 0.51 +1.0 59,031 + EX31_RS06445 0.51 -1.1 59,031 + EX31_RS06445 0.51 +0.9 59,031 + EX31_RS06445 0.51 -0.2 59,031 + EX31_RS06445 0.51 -0.4 59,031 + EX31_RS06445 0.51 -2.5 59,031 + EX31_RS06445 0.51 +0.8 59,032 - EX31_RS06445 0.51 +0.4 59,032 - EX31_RS06445 0.51 -1.0 59,032 - EX31_RS06445 0.51 +0.8 59,032 - EX31_RS06445 0.51 -0.0 59,130 + EX31_RS06445 0.60 -2.0 59,130 + EX31_RS06445 0.60 -1.0 59,130 + EX31_RS06445 0.60 +0.2 59,131 - EX31_RS06445 0.60 +0.7 59,132 + EX31_RS06445 0.60 +1.0 59,132 + EX31_RS06445 0.60 -0.0 59,132 + EX31_RS06445 0.60 +2.7 59,132 + EX31_RS06445 0.60 +0.9 59,133 - EX31_RS06445 0.60 +1.0 59,133 - EX31_RS06445 0.60 +0.9 59,133 - EX31_RS06445 0.60 -1.0 59,221 - EX31_RS06445 0.68 -1.3 59,221 - EX31_RS06445 0.68 -0.4 59,221 - EX31_RS06445 0.68 +0.9 59,221 - EX31_RS06445 0.68 +0.6 59,221 - EX31_RS06445 0.68 -1.0 59,232 - EX31_RS06445 0.69 +0.1 59,297 + EX31_RS06445 0.75 -1.3 59,355 + EX31_RS06445 0.80 -0.6 59,355 + EX31_RS06445 0.80 -0.8 59,355 + EX31_RS06445 0.80 -0.8 59,355 + EX31_RS06445 0.80 +0.7 59,355 + EX31_RS06445 0.80 +0.4 59,355 + EX31_RS06445 0.80 +0.9 59,355 + EX31_RS06445 0.80 +0.4 59,355 + EX31_RS06445 0.80 -0.1 59,355 + EX31_RS06445 0.80 +1.0 59,355 + EX31_RS06445 0.80 +0.5 59,356 - EX31_RS06445 0.81 +0.1 59,356 - EX31_RS06445 0.81 +0.3 59,356 - EX31_RS06445 0.81 +0.4 59,356 - EX31_RS06445 0.81 +0.7 59,356 - EX31_RS06445 0.81 -0.6 59,356 - EX31_RS06445 0.81 +0.4 59,356 - EX31_RS06445 0.81 -0.8 59,356 - EX31_RS06445 0.81 -0.5 59,407 + EX31_RS06445 0.85 -1.8 59,407 + EX31_RS06445 0.85 -2.0 59,408 - EX31_RS06445 0.85 -0.1 59,408 - EX31_RS06445 0.85 -0.4 59,498 + +0.7 59,499 - -0.8 59,568 + +0.4 59,568 + +0.1 59,568 + +0.9 59,568 + -1.1 59,568 + +0.5 59,569 - +0.7 59,569 - +0.0 59,569 - -1.1 59,569 - -0.9 59,598 + +1.8 59,599 - +0.3 59,608 + -0.5 59,608 + -0.3 59,609 - -1.0 59,609 - -0.1 59,636 - -1.0 59,724 + +0.9 59,732 + +1.4 59,732 + +0.1 59,733 - +0.9 59,733 - +0.4 59,733 - +0.4 59,733 - -1.2 59,768 - EX31_RS06450 0.10 -0.2 59,768 - EX31_RS06450 0.10 -1.1 59,768 - EX31_RS06450 0.10 -0.9 59,776 + EX31_RS06450 0.11 -0.9 59,812 + EX31_RS06450 0.14 -1.4 59,831 + EX31_RS06450 0.15 +1.4 59,843 + EX31_RS06450 0.16 -0.4 60,084 - EX31_RS06450 0.35 -0.9 60,084 - EX31_RS06450 0.35 +0.3
Or see this region's nucleotide sequence