Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03910 and EX31_RS03915 are separated by 142 nucleotides EX31_RS03915 and EX31_RS03920 are separated by 126 nucleotides EX31_RS03920 and EX31_RS03925 are separated by 275 nucleotides
EX31_RS03910: EX31_RS03910 - MFS transporter, at 297,159 to 298,481
_RS03910
EX31_RS03915: EX31_RS03915 - molybdopterin adenylyltransferase, at 298,624 to 299,211
_RS03915
EX31_RS03920: EX31_RS03920 - transaldolase, at 299,338 to 300,291
_RS03920
EX31_RS03925: EX31_RS03925 - alanine:cation symporter family protein, at 300,567 to 301,994
_RS03925
Position (kb) 299
300
301 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 298.363 kb on - strand at 298.363 kb on - strand at 298.481 kb on + strand at 298.481 kb on + strand at 298.481 kb on + strand at 298.481 kb on + strand at 298.481 kb on + strand at 298.481 kb on + strand at 298.481 kb on + strand at 298.481 kb on + strand at 298.481 kb on + strand at 298.481 kb on + strand at 298.481 kb on + strand at 298.481 kb on + strand at 298.482 kb on - strand at 298.482 kb on - strand at 298.482 kb on - strand at 298.482 kb on - strand at 298.504 kb on + strand at 298.532 kb on + strand at 298.548 kb on + strand at 298.593 kb on - strand at 298.621 kb on + strand at 298.622 kb on - strand at 298.694 kb on + strand, within EX31_RS03915 at 298.694 kb on + strand, within EX31_RS03915 at 298.694 kb on + strand, within EX31_RS03915 at 298.694 kb on + strand, within EX31_RS03915 at 298.694 kb on + strand, within EX31_RS03915 at 298.694 kb on + strand, within EX31_RS03915 at 298.694 kb on + strand, within EX31_RS03915 at 298.694 kb on + strand, within EX31_RS03915 at 298.695 kb on - strand, within EX31_RS03915 at 298.695 kb on - strand, within EX31_RS03915 at 298.695 kb on - strand, within EX31_RS03915 at 298.695 kb on - strand, within EX31_RS03915 at 298.695 kb on - strand, within EX31_RS03915 at 298.695 kb on - strand, within EX31_RS03915 at 298.695 kb on - strand, within EX31_RS03915 at 298.695 kb on - strand, within EX31_RS03915 at 298.695 kb on - strand, within EX31_RS03915 at 298.695 kb on - strand, within EX31_RS03915 at 298.695 kb on - strand, within EX31_RS03915 at 298.695 kb on - strand, within EX31_RS03915 at 298.695 kb on - strand, within EX31_RS03915 at 298.758 kb on + strand, within EX31_RS03915 at 298.759 kb on - strand, within EX31_RS03915 at 298.797 kb on + strand, within EX31_RS03915 at 298.797 kb on + strand, within EX31_RS03915 at 298.798 kb on - strand, within EX31_RS03915 at 298.798 kb on - strand, within EX31_RS03915 at 298.798 kb on - strand, within EX31_RS03915 at 298.817 kb on - strand, within EX31_RS03915 at 298.817 kb on - strand, within EX31_RS03915 at 298.828 kb on + strand, within EX31_RS03915 at 299.091 kb on + strand, within EX31_RS03915 at 299.091 kb on + strand, within EX31_RS03915 at 299.091 kb on + strand, within EX31_RS03915 at 299.092 kb on - strand, within EX31_RS03915 at 299.092 kb on - strand, within EX31_RS03915 at 299.092 kb on - strand, within EX31_RS03915 at 299.092 kb on - strand, within EX31_RS03915 at 299.100 kb on + strand, within EX31_RS03915 at 299.100 kb on + strand, within EX31_RS03915 at 299.100 kb on + strand, within EX31_RS03915 at 299.100 kb on + strand, within EX31_RS03915 at 299.100 kb on + strand, within EX31_RS03915 at 299.101 kb on - strand, within EX31_RS03915 at 299.101 kb on - strand, within EX31_RS03915 at 299.101 kb on - strand, within EX31_RS03915 at 299.103 kb on + strand, within EX31_RS03915 at 299.104 kb on - strand, within EX31_RS03915 at 299.104 kb on - strand, within EX31_RS03915 at 299.104 kb on - strand, within EX31_RS03915 at 299.185 kb on + strand at 299.201 kb on - strand at 299.205 kb on + strand at 299.205 kb on + strand at 299.205 kb on + strand at 299.205 kb on + strand at 299.205 kb on + strand at 299.206 kb on - strand at 299.206 kb on - strand at 299.206 kb on - strand at 299.206 kb on - strand at 299.206 kb on - strand at 299.206 kb on - strand at 299.206 kb on - strand at 299.206 kb on - strand at 299.206 kb on - strand at 299.206 kb on - strand at 299.214 kb on + strand at 299.214 kb on + strand at 299.215 kb on - strand at 299.215 kb on - strand at 299.239 kb on + strand at 299.240 kb on - strand at 299.248 kb on + strand at 299.248 kb on + strand at 299.248 kb on + strand at 299.248 kb on + strand at 299.249 kb on - strand at 299.255 kb on + strand at 299.255 kb on + strand at 299.255 kb on + strand at 299.255 kb on + strand at 299.255 kb on + strand at 299.255 kb on + strand at 299.255 kb on + strand at 299.256 kb on - strand at 299.256 kb on - strand at 299.256 kb on - strand at 299.256 kb on - strand at 299.256 kb on - strand at 299.308 kb on + strand at 299.317 kb on + strand at 299.317 kb on + strand at 299.318 kb on - strand at 299.318 kb on - strand at 299.339 kb on + strand at 299.371 kb on - strand at 299.371 kb on - strand at 299.392 kb on - strand at 299.392 kb on - strand at 299.392 kb on - strand at 299.448 kb on - strand, within EX31_RS03920 at 299.509 kb on - strand, within EX31_RS03920 at 299.509 kb on - strand, within EX31_RS03920 at 299.509 kb on - strand, within EX31_RS03920 at 299.509 kb on - strand, within EX31_RS03920 at 299.608 kb on - strand, within EX31_RS03920 at 299.655 kb on - strand, within EX31_RS03920 at 299.657 kb on + strand, within EX31_RS03920 at 299.657 kb on + strand, within EX31_RS03920 at 299.658 kb on - strand, within EX31_RS03920 at 299.658 kb on - strand, within EX31_RS03920 at 299.658 kb on - strand, within EX31_RS03920 at 299.664 kb on - strand, within EX31_RS03920 at 299.736 kb on - strand, within EX31_RS03920 at 299.736 kb on - strand, within EX31_RS03920 at 299.736 kb on - strand, within EX31_RS03920 at 299.736 kb on - strand, within EX31_RS03920 at 299.736 kb on - strand, within EX31_RS03920 at 299.887 kb on - strand, within EX31_RS03920 at 299.887 kb on - strand, within EX31_RS03920 at 299.907 kb on + strand, within EX31_RS03920 at 299.935 kb on - strand, within EX31_RS03920 at 299.936 kb on + strand, within EX31_RS03920 at 299.937 kb on - strand, within EX31_RS03920 at 299.937 kb on - strand, within EX31_RS03920 at 299.937 kb on - strand, within EX31_RS03920 at 299.937 kb on - strand, within EX31_RS03920 at 299.985 kb on - strand, within EX31_RS03920 at 299.985 kb on - strand, within EX31_RS03920 at 299.985 kb on - strand, within EX31_RS03920 at 299.985 kb on - strand, within EX31_RS03920 at 299.985 kb on - strand, within EX31_RS03920 at 300.082 kb on - strand, within EX31_RS03920 at 300.213 kb on - strand at 300.231 kb on + strand at 300.232 kb on - strand at 300.299 kb on + strand at 300.299 kb on + strand at 300.300 kb on + strand at 300.300 kb on - strand at 300.300 kb on - strand at 300.324 kb on - strand at 300.324 kb on - strand at 300.326 kb on - strand at 300.358 kb on + strand at 300.359 kb on - strand at 300.359 kb on - strand at 300.359 kb on - strand at 300.359 kb on - strand at 300.389 kb on - strand at 300.389 kb on - strand at 300.417 kb on - strand at 300.417 kb on - strand at 300.417 kb on - strand at 300.462 kb on + strand at 300.463 kb on - strand at 300.463 kb on - strand at 300.478 kb on + strand at 300.478 kb on + strand at 300.479 kb on - strand at 300.479 kb on - strand at 300.479 kb on - strand at 300.488 kb on - strand at 300.488 kb on - strand at 300.488 kb on - strand at 300.488 kb on - strand at 300.492 kb on + strand at 300.531 kb on - strand at 300.566 kb on + strand at 300.566 kb on + strand at 300.566 kb on + strand at 300.566 kb on + strand at 300.566 kb on + strand at 300.566 kb on + strand at 300.566 kb on + strand at 300.566 kb on + strand at 300.566 kb on + strand at 300.566 kb on + strand at 300.566 kb on + strand at 300.566 kb on + strand at 300.567 kb on - strand at 300.567 kb on - strand at 300.567 kb on - strand at 300.567 kb on - strand at 300.567 kb on - strand at 300.567 kb on - strand at 300.567 kb on - strand at 300.594 kb on - strand at 300.596 kb on + strand at 300.596 kb on + strand at 300.596 kb on + strand at 300.596 kb on + strand at 300.596 kb on + strand at 300.597 kb on - strand at 300.597 kb on - strand at 300.597 kb on - strand at 300.597 kb on - strand at 300.597 kb on - strand at 300.597 kb on - strand at 300.597 kb on - strand at 300.597 kb on - strand at 300.621 kb on + strand at 300.621 kb on + strand at 300.621 kb on + strand at 300.622 kb on - strand at 300.645 kb on + strand at 300.645 kb on + strand at 300.646 kb on - strand at 300.646 kb on - strand at 300.650 kb on + strand at 300.650 kb on + strand at 300.650 kb on + strand at 300.650 kb on + strand at 300.651 kb on - strand at 300.651 kb on - strand at 300.711 kb on + strand, within EX31_RS03925 at 300.712 kb on - strand, within EX31_RS03925 at 300.712 kb on - strand, within EX31_RS03925 at 300.712 kb on - strand, within EX31_RS03925 at 300.714 kb on - strand, within EX31_RS03925 at 300.737 kb on + strand, within EX31_RS03925 at 300.737 kb on + strand, within EX31_RS03925 at 300.737 kb on + strand, within EX31_RS03925 at 300.738 kb on - strand, within EX31_RS03925 at 300.738 kb on - strand, within EX31_RS03925 at 300.780 kb on - strand, within EX31_RS03925 at 300.857 kb on + strand, within EX31_RS03925 at 300.858 kb on - strand, within EX31_RS03925 at 300.858 kb on - strand, within EX31_RS03925 at 300.858 kb on - strand, within EX31_RS03925 at 300.889 kb on + strand, within EX31_RS03925 at 300.889 kb on + strand, within EX31_RS03925 at 300.890 kb on - strand, within EX31_RS03925 at 300.913 kb on - strand, within EX31_RS03925 at 300.913 kb on - strand, within EX31_RS03925 at 300.913 kb on - strand, within EX31_RS03925 at 300.913 kb on - strand, within EX31_RS03925 at 300.913 kb on - strand, within EX31_RS03925 at 300.925 kb on + strand, within EX31_RS03925 at 300.925 kb on + strand, within EX31_RS03925 at 300.925 kb on + strand, within EX31_RS03925 at 300.926 kb on - strand, within EX31_RS03925 at 300.926 kb on - strand, within EX31_RS03925 at 300.926 kb on - strand, within EX31_RS03925 at 300.926 kb on - strand, within EX31_RS03925 at 300.926 kb on - strand, within EX31_RS03925 at 300.926 kb on - strand, within EX31_RS03925 at 300.926 kb on - strand, within EX31_RS03925 at 300.926 kb on - strand, within EX31_RS03925 at 300.960 kb on + strand, within EX31_RS03925 at 300.960 kb on + strand, within EX31_RS03925 at 300.960 kb on + strand, within EX31_RS03925 at 300.960 kb on + strand, within EX31_RS03925 at 300.960 kb on + strand, within EX31_RS03925 at 300.960 kb on + strand, within EX31_RS03925 at 300.960 kb on + strand, within EX31_RS03925 at 300.961 kb on - strand, within EX31_RS03925 at 300.961 kb on - strand, within EX31_RS03925 at 300.995 kb on + strand, within EX31_RS03925 at 301.049 kb on - strand, within EX31_RS03925 at 301.093 kb on + strand, within EX31_RS03925 at 301.190 kb on - strand, within EX31_RS03925 at 301.190 kb on - strand, within EX31_RS03925 at 301.209 kb on + strand, within EX31_RS03925 at 301.209 kb on + strand, within EX31_RS03925 at 301.210 kb on - strand, within EX31_RS03925 at 301.210 kb on - strand, within EX31_RS03925
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 298,363 - -0.9 298,363 - -1.3 298,481 + +1.1 298,481 + +0.2 298,481 + +0.7 298,481 + +0.9 298,481 + +1.2 298,481 + +0.3 298,481 + -0.2 298,481 + +0.9 298,481 + +0.7 298,481 + -0.4 298,481 + +0.4 298,481 + +0.4 298,482 - -1.3 298,482 - -1.4 298,482 - +0.3 298,482 - +0.2 298,504 + +0.6 298,532 + +0.2 298,548 + -0.1 298,593 - +0.0 298,621 + +0.6 298,622 - +1.3 298,694 + EX31_RS03915 0.12 +0.3 298,694 + EX31_RS03915 0.12 +0.4 298,694 + EX31_RS03915 0.12 -0.3 298,694 + EX31_RS03915 0.12 +1.4 298,694 + EX31_RS03915 0.12 -0.7 298,694 + EX31_RS03915 0.12 +0.5 298,694 + EX31_RS03915 0.12 +0.3 298,694 + EX31_RS03915 0.12 -0.1 298,695 - EX31_RS03915 0.12 +0.2 298,695 - EX31_RS03915 0.12 +1.0 298,695 - EX31_RS03915 0.12 -0.0 298,695 - EX31_RS03915 0.12 +0.6 298,695 - EX31_RS03915 0.12 +0.1 298,695 - EX31_RS03915 0.12 -0.5 298,695 - EX31_RS03915 0.12 +0.9 298,695 - EX31_RS03915 0.12 +1.5 298,695 - EX31_RS03915 0.12 -1.0 298,695 - EX31_RS03915 0.12 -0.4 298,695 - EX31_RS03915 0.12 +0.8 298,695 - EX31_RS03915 0.12 -1.7 298,695 - EX31_RS03915 0.12 -0.2 298,758 + EX31_RS03915 0.23 +0.4 298,759 - EX31_RS03915 0.23 -0.4 298,797 + EX31_RS03915 0.29 +0.1 298,797 + EX31_RS03915 0.29 +0.1 298,798 - EX31_RS03915 0.30 +0.6 298,798 - EX31_RS03915 0.30 +1.2 298,798 - EX31_RS03915 0.30 -0.1 298,817 - EX31_RS03915 0.33 +1.2 298,817 - EX31_RS03915 0.33 +1.2 298,828 + EX31_RS03915 0.35 -0.3 299,091 + EX31_RS03915 0.79 -0.2 299,091 + EX31_RS03915 0.79 +0.7 299,091 + EX31_RS03915 0.79 +0.7 299,092 - EX31_RS03915 0.80 -0.3 299,092 - EX31_RS03915 0.80 -0.6 299,092 - EX31_RS03915 0.80 +0.4 299,092 - EX31_RS03915 0.80 -0.8 299,100 + EX31_RS03915 0.81 -0.1 299,100 + EX31_RS03915 0.81 -2.0 299,100 + EX31_RS03915 0.81 +0.7 299,100 + EX31_RS03915 0.81 -0.4 299,100 + EX31_RS03915 0.81 +0.8 299,101 - EX31_RS03915 0.81 -0.2 299,101 - EX31_RS03915 0.81 -0.6 299,101 - EX31_RS03915 0.81 -1.2 299,103 + EX31_RS03915 0.81 -1.6 299,104 - EX31_RS03915 0.82 -0.7 299,104 - EX31_RS03915 0.82 -0.3 299,104 - EX31_RS03915 0.82 +1.0 299,185 + +0.6 299,201 - +0.5 299,205 + -0.5 299,205 + +0.4 299,205 + +1.0 299,205 + -1.1 299,205 + +0.0 299,206 - +0.4 299,206 - -0.1 299,206 - -0.5 299,206 - -0.4 299,206 - +1.6 299,206 - -0.8 299,206 - -0.8 299,206 - -0.1 299,206 - -1.0 299,206 - -0.3 299,214 + +1.3 299,214 + +0.8 299,215 - +0.2 299,215 - -0.4 299,239 + +2.2 299,240 - -1.7 299,248 + -0.3 299,248 + -0.1 299,248 + -0.6 299,248 + +0.2 299,249 - -0.3 299,255 + -0.0 299,255 + +0.2 299,255 + -0.1 299,255 + -0.5 299,255 + -1.4 299,255 + +0.4 299,255 + +0.5 299,256 - -0.4 299,256 - -1.1 299,256 - +0.7 299,256 - +0.7 299,256 - +0.6 299,308 + +0.0 299,317 + -0.6 299,317 + -0.2 299,318 - -1.3 299,318 - -0.8 299,339 + -1.2 299,371 - -2.2 299,371 - +0.4 299,392 - -1.4 299,392 - +0.9 299,392 - -1.2 299,448 - EX31_RS03920 0.12 +0.1 299,509 - EX31_RS03920 0.18 -0.2 299,509 - EX31_RS03920 0.18 +0.3 299,509 - EX31_RS03920 0.18 -0.4 299,509 - EX31_RS03920 0.18 +0.3 299,608 - EX31_RS03920 0.28 -2.3 299,655 - EX31_RS03920 0.33 -1.0 299,657 + EX31_RS03920 0.33 +0.4 299,657 + EX31_RS03920 0.33 -0.8 299,658 - EX31_RS03920 0.34 -0.2 299,658 - EX31_RS03920 0.34 -1.5 299,658 - EX31_RS03920 0.34 +0.3 299,664 - EX31_RS03920 0.34 -1.0 299,736 - EX31_RS03920 0.42 -0.8 299,736 - EX31_RS03920 0.42 -1.4 299,736 - EX31_RS03920 0.42 -0.7 299,736 - EX31_RS03920 0.42 -0.2 299,736 - EX31_RS03920 0.42 -2.0 299,887 - EX31_RS03920 0.58 +0.6 299,887 - EX31_RS03920 0.58 -1.2 299,907 + EX31_RS03920 0.60 -0.1 299,935 - EX31_RS03920 0.63 -1.1 299,936 + EX31_RS03920 0.63 -0.7 299,937 - EX31_RS03920 0.63 -2.4 299,937 - EX31_RS03920 0.63 +0.8 299,937 - EX31_RS03920 0.63 -1.5 299,937 - EX31_RS03920 0.63 +1.2 299,985 - EX31_RS03920 0.68 -0.7 299,985 - EX31_RS03920 0.68 +0.1 299,985 - EX31_RS03920 0.68 -1.0 299,985 - EX31_RS03920 0.68 -0.5 299,985 - EX31_RS03920 0.68 +0.8 300,082 - EX31_RS03920 0.78 +0.4 300,213 - -0.2 300,231 + +0.6 300,232 - -1.5 300,299 + +0.3 300,299 + +0.0 300,300 + -0.7 300,300 - -0.1 300,300 - +0.3 300,324 - -0.8 300,324 - +0.7 300,326 - +0.2 300,358 + -0.8 300,359 - -1.7 300,359 - +0.6 300,359 - +0.3 300,359 - +0.7 300,389 - -0.7 300,389 - -0.1 300,417 - +0.1 300,417 - +1.2 300,417 - +0.4 300,462 + +0.9 300,463 - -0.7 300,463 - -0.7 300,478 + +0.3 300,478 + +0.4 300,479 - -0.8 300,479 - -0.3 300,479 - -0.6 300,488 - +0.3 300,488 - +0.3 300,488 - -0.5 300,488 - +0.2 300,492 + +0.3 300,531 - +1.2 300,566 + -0.7 300,566 + +0.8 300,566 + +0.4 300,566 + +1.3 300,566 + +0.8 300,566 + -0.2 300,566 + -1.1 300,566 + +0.4 300,566 + +2.4 300,566 + -0.1 300,566 + -1.0 300,566 + +0.0 300,567 - +0.4 300,567 - +0.2 300,567 - -0.0 300,567 - -0.2 300,567 - -1.8 300,567 - -0.1 300,567 - +0.9 300,594 - +0.8 300,596 + +0.8 300,596 + +1.5 300,596 + -0.9 300,596 + +0.3 300,596 + +1.6 300,597 - -0.7 300,597 - +0.2 300,597 - +1.2 300,597 - -0.3 300,597 - +2.0 300,597 - +1.3 300,597 - -1.0 300,597 - -0.3 300,621 + -0.4 300,621 + -0.8 300,621 + +0.8 300,622 - -0.2 300,645 + +0.6 300,645 + -0.9 300,646 - -0.7 300,646 - +0.7 300,650 + -1.1 300,650 + -0.2 300,650 + -0.9 300,650 + +0.4 300,651 - -0.5 300,651 - -0.5 300,711 + EX31_RS03925 0.10 +0.3 300,712 - EX31_RS03925 0.10 +0.1 300,712 - EX31_RS03925 0.10 +0.1 300,712 - EX31_RS03925 0.10 -1.5 300,714 - EX31_RS03925 0.10 +0.8 300,737 + EX31_RS03925 0.12 -0.5 300,737 + EX31_RS03925 0.12 -0.5 300,737 + EX31_RS03925 0.12 +1.0 300,738 - EX31_RS03925 0.12 -0.8 300,738 - EX31_RS03925 0.12 +1.0 300,780 - EX31_RS03925 0.15 +0.3 300,857 + EX31_RS03925 0.20 -1.0 300,858 - EX31_RS03925 0.20 +0.1 300,858 - EX31_RS03925 0.20 +0.0 300,858 - EX31_RS03925 0.20 +0.6 300,889 + EX31_RS03925 0.23 -0.5 300,889 + EX31_RS03925 0.23 +0.3 300,890 - EX31_RS03925 0.23 +0.1 300,913 - EX31_RS03925 0.24 -0.6 300,913 - EX31_RS03925 0.24 +0.0 300,913 - EX31_RS03925 0.24 -0.3 300,913 - EX31_RS03925 0.24 +0.7 300,913 - EX31_RS03925 0.24 +1.0 300,925 + EX31_RS03925 0.25 +0.2 300,925 + EX31_RS03925 0.25 -0.4 300,925 + EX31_RS03925 0.25 -1.0 300,926 - EX31_RS03925 0.25 +1.2 300,926 - EX31_RS03925 0.25 -1.0 300,926 - EX31_RS03925 0.25 +0.7 300,926 - EX31_RS03925 0.25 -0.8 300,926 - EX31_RS03925 0.25 +1.6 300,926 - EX31_RS03925 0.25 -0.2 300,926 - EX31_RS03925 0.25 -1.2 300,926 - EX31_RS03925 0.25 +2.2 300,960 + EX31_RS03925 0.28 +1.3 300,960 + EX31_RS03925 0.28 -1.2 300,960 + EX31_RS03925 0.28 -0.4 300,960 + EX31_RS03925 0.28 +0.6 300,960 + EX31_RS03925 0.28 +0.1 300,960 + EX31_RS03925 0.28 +1.7 300,960 + EX31_RS03925 0.28 -0.2 300,961 - EX31_RS03925 0.28 +0.3 300,961 - EX31_RS03925 0.28 -3.1 300,995 + EX31_RS03925 0.30 -0.6 301,049 - EX31_RS03925 0.34 -0.7 301,093 + EX31_RS03925 0.37 +0.2 301,190 - EX31_RS03925 0.44 -1.0 301,190 - EX31_RS03925 0.44 -0.9 301,209 + EX31_RS03925 0.45 +0.6 301,209 + EX31_RS03925 0.45 -0.2 301,210 - EX31_RS03925 0.45 -0.4 301,210 - EX31_RS03925 0.45 +0.0
Or see this region's nucleotide sequence