Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24475 and EX31_RS24480 are separated by 187 nucleotides EX31_RS24480 and EX31_RS24485 are separated by 8 nucleotides EX31_RS24485 and EX31_RS24490 are separated by 21 nucleotides EX31_RS24490 and EX31_RS24495 overlap by 17 nucleotides
EX31_RS24475: EX31_RS24475 - PTS glucose transporter subunit IIBC, at 662,630 to 664,063
_RS24475
EX31_RS24480: EX31_RS24480 - purine nucleoside phosphoramidase, at 664,251 to 664,604
_RS24480
EX31_RS24485: EX31_RS24485 - DUF1425 domain-containing protein, at 664,613 to 664,981
_RS24485
EX31_RS24490: EX31_RS24490 - penicillin-binding protein activator LpoB, at 665,003 to 665,605
_RS24490
EX31_RS24495: EX31_RS24495 - phosphotransferase, at 665,589 to 666,446
_RS24495
Position (kb) 664
665 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 663.265 kb on + strand, within EX31_RS24475 at 663.265 kb on + strand, within EX31_RS24475 at 663.302 kb on + strand, within EX31_RS24475 at 663.302 kb on + strand, within EX31_RS24475 at 663.302 kb on + strand, within EX31_RS24475 at 663.303 kb on - strand, within EX31_RS24475 at 663.303 kb on - strand, within EX31_RS24475 at 663.303 kb on - strand, within EX31_RS24475 at 663.347 kb on + strand, within EX31_RS24475 at 663.347 kb on + strand, within EX31_RS24475 at 663.347 kb on + strand, within EX31_RS24475 at 663.347 kb on + strand, within EX31_RS24475 at 663.347 kb on + strand, within EX31_RS24475 at 663.347 kb on + strand, within EX31_RS24475 at 663.347 kb on + strand, within EX31_RS24475 at 663.347 kb on + strand, within EX31_RS24475 at 663.347 kb on + strand, within EX31_RS24475 at 663.347 kb on + strand, within EX31_RS24475 at 663.347 kb on + strand, within EX31_RS24475 at 663.347 kb on + strand, within EX31_RS24475 at 663.347 kb on + strand, within EX31_RS24475 at 663.348 kb on - strand, within EX31_RS24475 at 663.350 kb on - strand, within EX31_RS24475 at 663.373 kb on + strand, within EX31_RS24475 at 663.373 kb on + strand, within EX31_RS24475 at 663.430 kb on + strand, within EX31_RS24475 at 663.563 kb on + strand, within EX31_RS24475 at 663.563 kb on + strand, within EX31_RS24475 at 663.564 kb on - strand, within EX31_RS24475 at 663.676 kb on + strand, within EX31_RS24475 at 663.708 kb on + strand, within EX31_RS24475 at 663.708 kb on + strand, within EX31_RS24475 at 663.708 kb on + strand, within EX31_RS24475 at 663.708 kb on + strand, within EX31_RS24475 at 663.708 kb on + strand, within EX31_RS24475 at 663.709 kb on - strand, within EX31_RS24475 at 663.719 kb on + strand, within EX31_RS24475 at 663.719 kb on + strand, within EX31_RS24475 at 663.719 kb on + strand, within EX31_RS24475 at 663.719 kb on + strand, within EX31_RS24475 at 663.720 kb on - strand, within EX31_RS24475 at 663.720 kb on - strand, within EX31_RS24475 at 663.731 kb on + strand, within EX31_RS24475 at 663.731 kb on + strand, within EX31_RS24475 at 663.734 kb on + strand, within EX31_RS24475 at 663.892 kb on + strand, within EX31_RS24475 at 663.892 kb on + strand, within EX31_RS24475 at 663.892 kb on + strand, within EX31_RS24475 at 663.892 kb on + strand, within EX31_RS24475 at 664.046 kb on + strand at 664.046 kb on + strand at 664.046 kb on + strand at 664.046 kb on + strand at 664.046 kb on + strand at 664.046 kb on + strand at 664.046 kb on + strand at 664.046 kb on + strand at 664.047 kb on - strand at 664.061 kb on + strand at 664.062 kb on - strand at 664.160 kb on + strand at 664.161 kb on - strand at 664.161 kb on - strand at 664.177 kb on - strand at 664.211 kb on - strand at 664.211 kb on - strand at 664.217 kb on + strand at 664.218 kb on - strand at 664.229 kb on + strand at 664.247 kb on + strand at 664.247 kb on + strand at 664.247 kb on + strand at 664.247 kb on + strand at 664.247 kb on + strand at 664.247 kb on + strand at 664.247 kb on + strand at 664.248 kb on - strand at 664.248 kb on - strand at 664.248 kb on - strand at 664.248 kb on - strand at 664.248 kb on - strand at 664.311 kb on + strand, within EX31_RS24480 at 664.367 kb on + strand, within EX31_RS24480 at 664.368 kb on - strand, within EX31_RS24480 at 664.542 kb on + strand, within EX31_RS24480 at 664.553 kb on + strand, within EX31_RS24480 at 664.553 kb on + strand, within EX31_RS24480 at 664.554 kb on - strand, within EX31_RS24480 at 664.653 kb on + strand, within EX31_RS24485 at 664.653 kb on + strand, within EX31_RS24485 at 664.653 kb on + strand, within EX31_RS24485 at 664.653 kb on + strand, within EX31_RS24485 at 664.653 kb on + strand, within EX31_RS24485 at 664.653 kb on + strand, within EX31_RS24485 at 664.653 kb on + strand, within EX31_RS24485 at 664.653 kb on + strand, within EX31_RS24485 at 664.654 kb on - strand, within EX31_RS24485 at 664.661 kb on - strand, within EX31_RS24485 at 664.755 kb on + strand, within EX31_RS24485 at 664.838 kb on + strand, within EX31_RS24485 at 664.838 kb on + strand, within EX31_RS24485 at 664.838 kb on + strand, within EX31_RS24485 at 664.838 kb on + strand, within EX31_RS24485 at 664.839 kb on - strand, within EX31_RS24485 at 664.844 kb on + strand, within EX31_RS24485 at 664.845 kb on - strand, within EX31_RS24485 at 664.849 kb on + strand, within EX31_RS24485 at 664.850 kb on - strand, within EX31_RS24485 at 664.850 kb on - strand, within EX31_RS24485 at 664.866 kb on + strand, within EX31_RS24485 at 664.894 kb on + strand, within EX31_RS24485 at 664.894 kb on + strand, within EX31_RS24485 at 664.894 kb on + strand, within EX31_RS24485 at 664.894 kb on + strand, within EX31_RS24485 at 664.895 kb on - strand, within EX31_RS24485 at 664.895 kb on - strand, within EX31_RS24485 at 664.952 kb on + strand at 664.952 kb on + strand at 664.953 kb on - strand at 664.963 kb on + strand at 664.963 kb on + strand at 664.964 kb on - strand at 664.964 kb on - strand at 664.964 kb on - strand at 664.964 kb on - strand at 664.967 kb on + strand at 664.967 kb on + strand at 664.967 kb on + strand at 664.973 kb on + strand at 664.974 kb on - strand at 665.056 kb on + strand at 665.056 kb on + strand at 665.056 kb on + strand at 665.056 kb on + strand at 665.057 kb on - strand at 665.057 kb on - strand at 665.057 kb on - strand at 665.478 kb on + strand, within EX31_RS24490 at 665.478 kb on + strand, within EX31_RS24490 at 665.479 kb on - strand, within EX31_RS24490 at 665.479 kb on - strand, within EX31_RS24490 at 665.489 kb on + strand, within EX31_RS24490 at 665.504 kb on - strand, within EX31_RS24490
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 663,265 + EX31_RS24475 0.44 -0.3 663,265 + EX31_RS24475 0.44 +0.8 663,302 + EX31_RS24475 0.47 +0.2 663,302 + EX31_RS24475 0.47 -0.2 663,302 + EX31_RS24475 0.47 +0.4 663,303 - EX31_RS24475 0.47 -0.7 663,303 - EX31_RS24475 0.47 +0.8 663,303 - EX31_RS24475 0.47 +0.7 663,347 + EX31_RS24475 0.50 +2.0 663,347 + EX31_RS24475 0.50 +0.5 663,347 + EX31_RS24475 0.50 -0.3 663,347 + EX31_RS24475 0.50 -0.7 663,347 + EX31_RS24475 0.50 -1.4 663,347 + EX31_RS24475 0.50 -1.0 663,347 + EX31_RS24475 0.50 -0.3 663,347 + EX31_RS24475 0.50 -0.1 663,347 + EX31_RS24475 0.50 +0.3 663,347 + EX31_RS24475 0.50 -0.3 663,347 + EX31_RS24475 0.50 +0.4 663,347 + EX31_RS24475 0.50 -1.2 663,347 + EX31_RS24475 0.50 -0.3 663,348 - EX31_RS24475 0.50 -0.2 663,350 - EX31_RS24475 0.50 +0.1 663,373 + EX31_RS24475 0.52 -1.8 663,373 + EX31_RS24475 0.52 -0.1 663,430 + EX31_RS24475 0.56 -1.0 663,563 + EX31_RS24475 0.65 +0.2 663,563 + EX31_RS24475 0.65 -0.0 663,564 - EX31_RS24475 0.65 +0.7 663,676 + EX31_RS24475 0.73 -0.8 663,708 + EX31_RS24475 0.75 +1.2 663,708 + EX31_RS24475 0.75 +0.3 663,708 + EX31_RS24475 0.75 -0.2 663,708 + EX31_RS24475 0.75 +0.7 663,708 + EX31_RS24475 0.75 +0.1 663,709 - EX31_RS24475 0.75 -0.2 663,719 + EX31_RS24475 0.76 -0.5 663,719 + EX31_RS24475 0.76 -0.7 663,719 + EX31_RS24475 0.76 -0.7 663,719 + EX31_RS24475 0.76 -1.5 663,720 - EX31_RS24475 0.76 +0.5 663,720 - EX31_RS24475 0.76 -0.1 663,731 + EX31_RS24475 0.77 -0.1 663,731 + EX31_RS24475 0.77 -2.7 663,734 + EX31_RS24475 0.77 -1.7 663,892 + EX31_RS24475 0.88 +0.8 663,892 + EX31_RS24475 0.88 -1.3 663,892 + EX31_RS24475 0.88 -1.1 663,892 + EX31_RS24475 0.88 -0.4 664,046 + -0.9 664,046 + +1.7 664,046 + +0.1 664,046 + -0.1 664,046 + +0.3 664,046 + -0.3 664,046 + -0.9 664,046 + +0.2 664,047 - +0.2 664,061 + +1.0 664,062 - +1.1 664,160 + -0.8 664,161 - +2.7 664,161 - +1.7 664,177 - -1.8 664,211 - +1.7 664,211 - -1.0 664,217 + -0.2 664,218 - +1.2 664,229 + +0.8 664,247 + +1.1 664,247 + +2.2 664,247 + -0.3 664,247 + +0.1 664,247 + +0.1 664,247 + -1.0 664,247 + -0.1 664,248 - +0.1 664,248 - -0.2 664,248 - +0.5 664,248 - -1.3 664,248 - +0.9 664,311 + EX31_RS24480 0.17 -1.7 664,367 + EX31_RS24480 0.33 -0.9 664,368 - EX31_RS24480 0.33 -0.1 664,542 + EX31_RS24480 0.82 +0.7 664,553 + EX31_RS24480 0.85 -0.3 664,553 + EX31_RS24480 0.85 -0.9 664,554 - EX31_RS24480 0.86 +0.8 664,653 + EX31_RS24485 0.11 -0.7 664,653 + EX31_RS24485 0.11 -0.6 664,653 + EX31_RS24485 0.11 +1.3 664,653 + EX31_RS24485 0.11 -0.7 664,653 + EX31_RS24485 0.11 +0.7 664,653 + EX31_RS24485 0.11 +1.1 664,653 + EX31_RS24485 0.11 -1.1 664,653 + EX31_RS24485 0.11 -0.6 664,654 - EX31_RS24485 0.11 +1.1 664,661 - EX31_RS24485 0.13 +0.1 664,755 + EX31_RS24485 0.38 -0.3 664,838 + EX31_RS24485 0.61 +0.1 664,838 + EX31_RS24485 0.61 +0.5 664,838 + EX31_RS24485 0.61 +0.7 664,838 + EX31_RS24485 0.61 +1.9 664,839 - EX31_RS24485 0.61 +0.7 664,844 + EX31_RS24485 0.63 +0.1 664,845 - EX31_RS24485 0.63 +1.1 664,849 + EX31_RS24485 0.64 +0.5 664,850 - EX31_RS24485 0.64 +0.2 664,850 - EX31_RS24485 0.64 -0.7 664,866 + EX31_RS24485 0.69 +0.2 664,894 + EX31_RS24485 0.76 +0.2 664,894 + EX31_RS24485 0.76 +1.7 664,894 + EX31_RS24485 0.76 +0.2 664,894 + EX31_RS24485 0.76 +1.2 664,895 - EX31_RS24485 0.76 -0.2 664,895 - EX31_RS24485 0.76 +0.7 664,952 + -3.0 664,952 + +0.9 664,953 - +0.3 664,963 + +1.2 664,963 + +0.2 664,964 - +0.9 664,964 - +0.3 664,964 - +0.6 664,964 - -2.7 664,967 + +0.4 664,967 + +1.2 664,967 + +0.2 664,973 + +1.4 664,974 - +1.6 665,056 + +1.4 665,056 + +0.6 665,056 + -0.3 665,056 + -0.3 665,057 - -0.6 665,057 - +0.3 665,057 - -0.7 665,478 + EX31_RS24490 0.79 +0.9 665,478 + EX31_RS24490 0.79 +0.4 665,479 - EX31_RS24490 0.79 +0.5 665,479 - EX31_RS24490 0.79 +0.2 665,489 + EX31_RS24490 0.81 +0.1 665,504 - EX31_RS24490 0.83 -0.6
Or see this region's nucleotide sequence