Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23435 and EX31_RS23440 are separated by 400 nucleotides EX31_RS23440 and EX31_RS23445 are separated by 46 nucleotides
EX31_RS23435: EX31_RS23435 - succinate dehydrogenase iron-sulfur subunit, at 453,259 to 453,975
_RS23435
EX31_RS23440: EX31_RS23440 - 2-oxoglutarate dehydrogenase E1 component, at 454,376 to 457,183
_RS23440
EX31_RS23445: EX31_RS23445 - 2-oxoglutarate dehydrogenase complex dihydrolipoyllysine-residue succinyltransferase, at 457,230 to 458,459
_RS23445
Position (kb)
454
455
456
457
458 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 453.483 kb on + strand, within EX31_RS23435 at 453.483 kb on + strand, within EX31_RS23435 at 453.483 kb on + strand, within EX31_RS23435 at 453.570 kb on - strand, within EX31_RS23435 at 453.572 kb on + strand, within EX31_RS23435 at 453.572 kb on + strand, within EX31_RS23435 at 453.572 kb on + strand, within EX31_RS23435 at 453.589 kb on + strand, within EX31_RS23435 at 453.609 kb on + strand, within EX31_RS23435 at 453.616 kb on + strand, within EX31_RS23435 at 453.616 kb on + strand, within EX31_RS23435 at 453.616 kb on + strand, within EX31_RS23435 at 453.616 kb on + strand, within EX31_RS23435 at 453.697 kb on + strand, within EX31_RS23435 at 453.697 kb on + strand, within EX31_RS23435 at 453.705 kb on + strand, within EX31_RS23435 at 453.705 kb on + strand, within EX31_RS23435 at 453.705 kb on + strand, within EX31_RS23435 at 453.706 kb on - strand, within EX31_RS23435 at 453.902 kb on + strand, within EX31_RS23435 at 453.903 kb on - strand, within EX31_RS23435 at 453.945 kb on + strand at 453.945 kb on + strand at 453.946 kb on - strand at 453.946 kb on - strand at 453.959 kb on + strand at 453.959 kb on + strand at 453.980 kb on + strand at 453.980 kb on + strand at 454.020 kb on + strand at 454.020 kb on + strand at 454.021 kb on - strand at 454.021 kb on - strand at 454.048 kb on - strand at 454.062 kb on + strand at 454.062 kb on + strand at 454.143 kb on + strand at 454.207 kb on + strand at 454.207 kb on + strand at 454.207 kb on + strand at 454.305 kb on + strand at 454.305 kb on + strand at 454.315 kb on + strand at 454.315 kb on + strand at 454.327 kb on + strand at 454.327 kb on + strand at 454.327 kb on + strand at 454.330 kb on + strand at 454.330 kb on + strand at 454.349 kb on + strand at 454.463 kb on + strand at 454.889 kb on + strand, within EX31_RS23440 at 454.900 kb on + strand, within EX31_RS23440 at 454.900 kb on + strand, within EX31_RS23440 at 454.993 kb on + strand, within EX31_RS23440 at 455.536 kb on + strand, within EX31_RS23440 at 455.545 kb on + strand, within EX31_RS23440 at 455.605 kb on + strand, within EX31_RS23440 at 455.779 kb on + strand, within EX31_RS23440 at 455.801 kb on + strand, within EX31_RS23440 at 455.937 kb on + strand, within EX31_RS23440 at 455.987 kb on + strand, within EX31_RS23440 at 456.313 kb on + strand, within EX31_RS23440 at 456.653 kb on + strand, within EX31_RS23440 at 457.077 kb on + strand at 457.204 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 453,483 + EX31_RS23435 0.31 -0.0 453,483 + EX31_RS23435 0.31 -0.1 453,483 + EX31_RS23435 0.31 +2.1 453,570 - EX31_RS23435 0.43 +0.7 453,572 + EX31_RS23435 0.44 -0.9 453,572 + EX31_RS23435 0.44 -1.9 453,572 + EX31_RS23435 0.44 +0.6 453,589 + EX31_RS23435 0.46 +1.0 453,609 + EX31_RS23435 0.49 +0.5 453,616 + EX31_RS23435 0.50 +1.1 453,616 + EX31_RS23435 0.50 -0.7 453,616 + EX31_RS23435 0.50 -0.9 453,616 + EX31_RS23435 0.50 +0.9 453,697 + EX31_RS23435 0.61 +0.4 453,697 + EX31_RS23435 0.61 +1.0 453,705 + EX31_RS23435 0.62 +2.0 453,705 + EX31_RS23435 0.62 -0.5 453,705 + EX31_RS23435 0.62 -0.7 453,706 - EX31_RS23435 0.62 -2.0 453,902 + EX31_RS23435 0.90 -1.7 453,903 - EX31_RS23435 0.90 +0.7 453,945 + -0.5 453,945 + -1.0 453,946 - +0.7 453,946 - +1.3 453,959 + +0.1 453,959 + +0.4 453,980 + +0.6 453,980 + +0.6 454,020 + +0.8 454,020 + -1.7 454,021 - -0.5 454,021 - +0.7 454,048 - -0.3 454,062 + +0.6 454,062 + +1.2 454,143 + -0.0 454,207 + +0.3 454,207 + +0.3 454,207 + -1.4 454,305 + +0.1 454,305 + +0.4 454,315 + -0.9 454,315 + -0.6 454,327 + -2.7 454,327 + -0.4 454,327 + -0.6 454,330 + -1.1 454,330 + -1.1 454,349 + +0.5 454,463 + -0.6 454,889 + EX31_RS23440 0.18 -0.9 454,900 + EX31_RS23440 0.19 +0.7 454,900 + EX31_RS23440 0.19 -0.6 454,993 + EX31_RS23440 0.22 -1.4 455,536 + EX31_RS23440 0.41 -2.5 455,545 + EX31_RS23440 0.42 -2.3 455,605 + EX31_RS23440 0.44 +0.1 455,779 + EX31_RS23440 0.50 +1.7 455,801 + EX31_RS23440 0.51 -0.9 455,937 + EX31_RS23440 0.56 -1.1 455,987 + EX31_RS23440 0.57 -1.6 456,313 + EX31_RS23440 0.69 -2.2 456,653 + EX31_RS23440 0.81 -1.1 457,077 + -2.4 457,204 + +0.3
Or see this region's nucleotide sequence