Strain Fitness in Rahnella sp. WP5 around EX31_RS16635

Experiment: L-Glutamine (N); ShewMM media; shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS16630 and EX31_RS16635 are separated by 16 nucleotidesEX31_RS16635 and EX31_RS16640 overlap by 4 nucleotides EX31_RS16630: EX31_RS16630 - phosphogluconate dehydratase, at 8,117 to 9,931 _RS16630 EX31_RS16635: EX31_RS16635 - gluconate transporter, at 9,948 to 11,285 _RS16635 EX31_RS16640: EX31_RS16640 - gluconokinase, at 11,282 to 11,821 _RS16640 Position (kb) 9 10 11 12Strain fitness (log2 ratio) -2 -1 0 1 2 3at 9.105 kb on + strand, within EX31_RS16630at 9.106 kb on - strand, within EX31_RS16630at 9.106 kb on - strand, within EX31_RS16630at 9.339 kb on + strand, within EX31_RS16630at 9.340 kb on - strand, within EX31_RS16630at 9.447 kb on - strand, within EX31_RS16630at 9.473 kb on - strand, within EX31_RS16630at 9.473 kb on - strand, within EX31_RS16630at 9.481 kb on - strand, within EX31_RS16630at 9.719 kb on - strand, within EX31_RS16630at 9.742 kb on + strand, within EX31_RS16630at 9.932 kb on - strandat 9.932 kb on - strandat 9.932 kb on - strandat 9.932 kb on - strandat 10.086 kb on + strand, within EX31_RS16635at 10.180 kb on - strand, within EX31_RS16635at 10.234 kb on - strand, within EX31_RS16635at 10.234 kb on - strand, within EX31_RS16635at 10.234 kb on - strand, within EX31_RS16635at 10.342 kb on - strand, within EX31_RS16635at 10.484 kb on + strand, within EX31_RS16635at 10.622 kb on + strand, within EX31_RS16635at 10.623 kb on - strand, within EX31_RS16635at 10.685 kb on + strand, within EX31_RS16635at 10.979 kb on - strand, within EX31_RS16635at 10.979 kb on - strand, within EX31_RS16635at 10.979 kb on - strand, within EX31_RS16635at 11.010 kb on + strand, within EX31_RS16635at 11.011 kb on - strand, within EX31_RS16635at 11.142 kb on - strand, within EX31_RS16635at 11.211 kb on - strandat 11.211 kb on - strandat 11.211 kb on - strandat 11.280 kb on - strandat 11.280 kb on - strandat 11.312 kb on - strandat 11.498 kb on + strand, within EX31_RS16640at 11.498 kb on + strand, within EX31_RS16640at 11.499 kb on - strand, within EX31_RS16640at 11.499 kb on - strand, within EX31_RS16640at 11.502 kb on + strand, within EX31_RS16640at 11.502 kb on + strand, within EX31_RS16640at 11.502 kb on + strand, within EX31_RS16640at 11.503 kb on - strand, within EX31_RS16640at 11.587 kb on + strand, within EX31_RS16640at 11.588 kb on - strand, within EX31_RS16640at 11.588 kb on - strand, within EX31_RS16640at 11.588 kb on - strand, within EX31_RS16640at 11.588 kb on - strand, within EX31_RS16640at 11.686 kb on + strand, within EX31_RS16640at 11.686 kb on + strand, within EX31_RS16640at 11.689 kb on + strand, within EX31_RS16640at 11.703 kb on - strand, within EX31_RS16640at 11.796 kb on + strandat 11.901 kb on - strandat 11.989 kb on - strandat 11.989 kb on - strandat 12.030 kb on + strandat 12.082 kb on + strandat 12.096 kb on + strandat 12.191 kb on + strandat 12.195 kb on - strandat 12.270 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking
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9,105 + EX31_RS16630 0.54 +2.0
9,106 - EX31_RS16630 0.54 -1.3
9,106 - EX31_RS16630 0.54 +0.6
9,339 + EX31_RS16630 0.67 -2.3
9,340 - EX31_RS16630 0.67 +1.0
9,447 - EX31_RS16630 0.73 -0.2
9,473 - EX31_RS16630 0.75 +0.7
9,473 - EX31_RS16630 0.75 -2.3
9,481 - EX31_RS16630 0.75 +1.1
9,719 - EX31_RS16630 0.88 +0.7
9,742 + EX31_RS16630 0.90 +1.5
9,932 - -0.1
9,932 - -0.1
9,932 - +2.0
9,932 - +0.0
10,086 + EX31_RS16635 0.10 -0.4
10,180 - EX31_RS16635 0.17 -0.2
10,234 - EX31_RS16635 0.21 +0.3
10,234 - EX31_RS16635 0.21 -1.2
10,234 - EX31_RS16635 0.21 -0.2
10,342 - EX31_RS16635 0.29 -1.1
10,484 + EX31_RS16635 0.40 -0.0
10,622 + EX31_RS16635 0.50 +0.9
10,623 - EX31_RS16635 0.50 +0.6
10,685 + EX31_RS16635 0.55 -1.8
10,979 - EX31_RS16635 0.77 -0.3
10,979 - EX31_RS16635 0.77 +1.5
10,979 - EX31_RS16635 0.77 -1.3
11,010 + EX31_RS16635 0.79 -0.9
11,011 - EX31_RS16635 0.79 -1.8
11,142 - EX31_RS16635 0.89 +0.5
11,211 - -2.0
11,211 - -0.5
11,211 - -0.9
11,280 - +1.5
11,280 - -1.0
11,312 - +1.9
11,498 + EX31_RS16640 0.40 -0.0
11,498 + EX31_RS16640 0.40 +1.3
11,499 - EX31_RS16640 0.40 +0.0
11,499 - EX31_RS16640 0.40 +0.8
11,502 + EX31_RS16640 0.41 +0.5
11,502 + EX31_RS16640 0.41 +1.7
11,502 + EX31_RS16640 0.41 -1.2
11,503 - EX31_RS16640 0.41 +1.6
11,587 + EX31_RS16640 0.56 +0.5
11,588 - EX31_RS16640 0.57 -0.7
11,588 - EX31_RS16640 0.57 +0.8
11,588 - EX31_RS16640 0.57 +0.7
11,588 - EX31_RS16640 0.57 +1.7
11,686 + EX31_RS16640 0.75 -0.4
11,686 + EX31_RS16640 0.75 -1.3
11,689 + EX31_RS16640 0.75 -0.1
11,703 - EX31_RS16640 0.78 +0.1
11,796 + -1.7
11,901 - +0.8
11,989 - +0.9
11,989 - +0.3
12,030 + +1.5
12,082 + -0.0
12,096 + -0.5
12,191 + -0.5
12,195 - -0.1
12,270 - +3.0

Or see this region's nucleotide sequence