Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15150 and EX31_RS25380 are separated by 158 nucleotides EX31_RS25380 and EX31_RS15155 are separated by 96 nucleotides EX31_RS15155 and EX31_RS15160 overlap by 4 nucleotides EX31_RS15160 and EX31_RS15165 overlap by 4 nucleotides
EX31_RS15150: EX31_RS15150 - class I adenylate cyclase, at 45,306 to 47,864
_RS15150
EX31_RS25380: EX31_RS25380 - hypothetical protein, at 48,023 to 48,226
_RS25380
EX31_RS15155: EX31_RS15155 - diaminopimelate epimerase, at 48,323 to 49,147
_RS15155
EX31_RS15160: EX31_RS15160 - DUF484 domain-containing protein, at 49,144 to 49,848
_RS15160
EX31_RS15165: EX31_RS15165 - tyrosine recombinase XerC, at 49,845 to 50,756
_RS15165
Position (kb)
48
49
50 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 47.425 kb on - strand, within EX31_RS15150 at 47.425 kb on - strand, within EX31_RS15150 at 47.570 kb on + strand, within EX31_RS15150 at 47.573 kb on + strand, within EX31_RS15150 at 47.574 kb on - strand, within EX31_RS15150 at 47.577 kb on + strand, within EX31_RS15150 at 47.577 kb on + strand, within EX31_RS15150 at 47.577 kb on + strand, within EX31_RS15150 at 47.577 kb on + strand, within EX31_RS15150 at 47.578 kb on - strand, within EX31_RS15150 at 47.578 kb on - strand, within EX31_RS15150 at 47.578 kb on - strand, within EX31_RS15150 at 47.578 kb on - strand, within EX31_RS15150 at 47.610 kb on + strand at 47.610 kb on + strand at 47.610 kb on + strand at 47.610 kb on + strand at 47.611 kb on - strand at 47.664 kb on + strand at 47.665 kb on - strand at 47.706 kb on - strand at 47.862 kb on + strand at 47.863 kb on - strand at 47.863 kb on - strand at 47.863 kb on - strand at 47.878 kb on - strand at 47.899 kb on - strand at 47.918 kb on + strand at 47.918 kb on + strand at 47.918 kb on + strand at 47.985 kb on + strand at 47.985 kb on + strand at 47.985 kb on + strand at 47.985 kb on + strand at 47.985 kb on + strand at 47.986 kb on - strand at 47.986 kb on - strand at 48.007 kb on + strand at 48.007 kb on + strand at 48.019 kb on + strand at 48.036 kb on + strand at 48.036 kb on + strand at 48.060 kb on + strand, within EX31_RS25380 at 48.088 kb on + strand, within EX31_RS25380 at 48.088 kb on + strand, within EX31_RS25380 at 48.088 kb on + strand, within EX31_RS25380 at 48.088 kb on + strand, within EX31_RS25380 at 48.088 kb on + strand, within EX31_RS25380 at 48.088 kb on + strand, within EX31_RS25380 at 48.088 kb on + strand, within EX31_RS25380 at 48.101 kb on + strand, within EX31_RS25380 at 48.101 kb on + strand, within EX31_RS25380 at 48.127 kb on + strand, within EX31_RS25380 at 48.476 kb on + strand, within EX31_RS15155 at 48.604 kb on + strand, within EX31_RS15155 at 48.605 kb on - strand, within EX31_RS15155 at 48.738 kb on - strand, within EX31_RS15155 at 48.765 kb on + strand, within EX31_RS15155 at 48.804 kb on + strand, within EX31_RS15155 at 48.927 kb on + strand, within EX31_RS15155 at 49.097 kb on + strand at 49.099 kb on + strand at 49.099 kb on + strand at 49.135 kb on + strand at 49.135 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.144 kb on - strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.179 kb on - strand at 49.179 kb on - strand at 49.179 kb on - strand at 49.179 kb on - strand at 49.179 kb on - strand at 49.179 kb on - strand at 49.207 kb on + strand at 49.207 kb on + strand at 49.207 kb on + strand at 49.207 kb on + strand at 49.207 kb on + strand at 49.356 kb on + strand, within EX31_RS15160 at 49.356 kb on + strand, within EX31_RS15160 at 49.356 kb on + strand, within EX31_RS15160 at 49.356 kb on + strand, within EX31_RS15160 at 49.357 kb on - strand, within EX31_RS15160 at 49.452 kb on + strand, within EX31_RS15160 at 49.652 kb on + strand, within EX31_RS15160 at 49.653 kb on - strand, within EX31_RS15160 at 49.661 kb on + strand, within EX31_RS15160 at 49.662 kb on - strand, within EX31_RS15160 at 49.787 kb on + strand at 49.844 kb on + strand at 49.897 kb on - strand at 49.929 kb on - strand at 49.941 kb on + strand, within EX31_RS15165 at 49.941 kb on + strand, within EX31_RS15165 at 49.941 kb on + strand, within EX31_RS15165 at 49.942 kb on - strand, within EX31_RS15165 at 49.942 kb on - strand, within EX31_RS15165 at 49.942 kb on - strand, within EX31_RS15165 at 49.942 kb on - strand, within EX31_RS15165 at 49.954 kb on + strand, within EX31_RS15165 at 49.955 kb on - strand, within EX31_RS15165 at 49.988 kb on + strand, within EX31_RS15165 at 50.046 kb on - strand, within EX31_RS15165
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 47,425 - EX31_RS15150 0.83 -0.3 47,425 - EX31_RS15150 0.83 +0.7 47,570 + EX31_RS15150 0.88 +0.4 47,573 + EX31_RS15150 0.89 +0.2 47,574 - EX31_RS15150 0.89 +0.8 47,577 + EX31_RS15150 0.89 +0.9 47,577 + EX31_RS15150 0.89 +0.9 47,577 + EX31_RS15150 0.89 +0.7 47,577 + EX31_RS15150 0.89 +0.6 47,578 - EX31_RS15150 0.89 +1.9 47,578 - EX31_RS15150 0.89 +1.0 47,578 - EX31_RS15150 0.89 +0.8 47,578 - EX31_RS15150 0.89 +1.0 47,610 + +1.2 47,610 + -0.5 47,610 + +0.6 47,610 + +1.0 47,611 - +1.5 47,664 + +1.1 47,665 - -0.3 47,706 - -0.9 47,862 + +0.2 47,863 - +0.5 47,863 - +1.7 47,863 - +1.0 47,878 - -1.1 47,899 - -0.6 47,918 + +0.3 47,918 + +0.6 47,918 + +0.1 47,985 + +0.5 47,985 + -0.2 47,985 + -0.5 47,985 + +0.8 47,985 + +1.9 47,986 - +3.5 47,986 - -1.1 48,007 + -0.6 48,007 + -0.1 48,019 + +0.1 48,036 + +1.5 48,036 + +0.2 48,060 + EX31_RS25380 0.18 -0.8 48,088 + EX31_RS25380 0.32 +0.1 48,088 + EX31_RS25380 0.32 -1.0 48,088 + EX31_RS25380 0.32 +1.5 48,088 + EX31_RS25380 0.32 +0.3 48,088 + EX31_RS25380 0.32 -0.0 48,088 + EX31_RS25380 0.32 -0.7 48,088 + EX31_RS25380 0.32 -0.2 48,101 + EX31_RS25380 0.38 -0.1 48,101 + EX31_RS25380 0.38 +1.0 48,127 + EX31_RS25380 0.51 -0.1 48,476 + EX31_RS15155 0.19 -1.0 48,604 + EX31_RS15155 0.34 +0.1 48,605 - EX31_RS15155 0.34 +1.2 48,738 - EX31_RS15155 0.50 +0.5 48,765 + EX31_RS15155 0.54 +0.6 48,804 + EX31_RS15155 0.58 -3.2 48,927 + EX31_RS15155 0.73 -1.6 49,097 + -1.6 49,099 + -0.5 49,099 + -0.4 49,135 + -0.9 49,135 + +0.3 49,143 + -0.1 49,143 + +0.0 49,143 + -0.7 49,143 + +1.6 49,143 + +0.8 49,143 + +0.2 49,143 + -0.1 49,143 + +0.1 49,143 + -1.4 49,143 + -1.0 49,143 + +0.0 49,143 + -0.3 49,144 - +0.5 49,178 + +0.4 49,178 + +1.1 49,178 + -0.9 49,178 + -1.0 49,178 + -0.1 49,178 + +0.1 49,178 + -0.4 49,178 + +0.1 49,178 + +0.0 49,178 + -0.1 49,179 - +0.0 49,179 - +0.5 49,179 - +0.4 49,179 - -1.0 49,179 - +0.7 49,179 - -0.2 49,207 + -0.3 49,207 + -0.4 49,207 + -0.5 49,207 + -0.5 49,207 + -0.1 49,356 + EX31_RS15160 0.30 -0.7 49,356 + EX31_RS15160 0.30 -0.8 49,356 + EX31_RS15160 0.30 -0.2 49,356 + EX31_RS15160 0.30 +1.5 49,357 - EX31_RS15160 0.30 -0.1 49,452 + EX31_RS15160 0.44 -0.6 49,652 + EX31_RS15160 0.72 -0.3 49,653 - EX31_RS15160 0.72 +1.2 49,661 + EX31_RS15160 0.73 -0.3 49,662 - EX31_RS15160 0.73 -0.5 49,787 + +0.4 49,844 + -0.4 49,897 - +0.6 49,929 - -0.0 49,941 + EX31_RS15165 0.11 +1.6 49,941 + EX31_RS15165 0.11 -1.5 49,941 + EX31_RS15165 0.11 -0.3 49,942 - EX31_RS15165 0.11 -0.3 49,942 - EX31_RS15165 0.11 -1.1 49,942 - EX31_RS15165 0.11 +1.1 49,942 - EX31_RS15165 0.11 -0.9 49,954 + EX31_RS15165 0.12 +0.5 49,955 - EX31_RS15165 0.12 -0.2 49,988 + EX31_RS15165 0.16 +0.4 50,046 - EX31_RS15165 0.22 -1.9
Or see this region's nucleotide sequence