Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10450 and EX31_RS10455 are separated by 27 nucleotides EX31_RS10455 and EX31_RS10460 are separated by 36 nucleotides
EX31_RS10450: EX31_RS10450 - IS110 family transposase, at 1 to 295
_RS10450
EX31_RS10455: EX31_RS10455 - filamentous hemagglutinin N-terminal domain-containing protein, at 323 to 3,697
_RS10455
EX31_RS10460: EX31_RS10460 - ShlB/FhaC/HecB family hemolysin secretion/activation protein, at 3,734 to 5,458
_RS10460
Position (kb) 0
1
2
3
4 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 0.445 kb on + strand at 0.803 kb on + strand, within EX31_RS10455 at 0.852 kb on - strand, within EX31_RS10455 at 0.852 kb on - strand, within EX31_RS10455 at 0.852 kb on - strand, within EX31_RS10455 at 0.887 kb on - strand, within EX31_RS10455 at 1.013 kb on + strand, within EX31_RS10455 at 1.013 kb on + strand, within EX31_RS10455 at 1.106 kb on - strand, within EX31_RS10455 at 1.114 kb on + strand, within EX31_RS10455 at 1.115 kb on - strand, within EX31_RS10455 at 1.372 kb on + strand, within EX31_RS10455 at 1.418 kb on + strand, within EX31_RS10455 at 1.426 kb on + strand, within EX31_RS10455 at 1.426 kb on + strand, within EX31_RS10455 at 1.426 kb on + strand, within EX31_RS10455 at 1.426 kb on + strand, within EX31_RS10455 at 1.427 kb on - strand, within EX31_RS10455 at 1.427 kb on - strand, within EX31_RS10455 at 1.657 kb on + strand, within EX31_RS10455 at 1.658 kb on - strand, within EX31_RS10455 at 1.903 kb on + strand, within EX31_RS10455 at 1.903 kb on + strand, within EX31_RS10455 at 1.904 kb on - strand, within EX31_RS10455 at 1.904 kb on - strand, within EX31_RS10455 at 2.413 kb on + strand, within EX31_RS10455 at 2.605 kb on + strand, within EX31_RS10455 at 2.677 kb on + strand, within EX31_RS10455 at 2.677 kb on + strand, within EX31_RS10455 at 2.819 kb on - strand, within EX31_RS10455 at 2.935 kb on - strand, within EX31_RS10455 at 2.935 kb on - strand, within EX31_RS10455 at 2.935 kb on - strand, within EX31_RS10455 at 2.938 kb on + strand, within EX31_RS10455 at 2.938 kb on + strand, within EX31_RS10455 at 2.939 kb on - strand, within EX31_RS10455 at 3.032 kb on + strand, within EX31_RS10455 at 3.032 kb on + strand, within EX31_RS10455 at 3.032 kb on + strand, within EX31_RS10455 at 3.033 kb on - strand, within EX31_RS10455 at 3.033 kb on - strand, within EX31_RS10455 at 3.264 kb on + strand, within EX31_RS10455 at 3.486 kb on + strand at 3.486 kb on + strand at 3.487 kb on - strand at 3.589 kb on + strand at 3.717 kb on + strand at 3.727 kb on + strand at 3.727 kb on + strand at 3.727 kb on + strand at 3.728 kb on - strand at 3.728 kb on - strand at 3.735 kb on + strand at 3.759 kb on + strand at 3.872 kb on + strand at 3.991 kb on + strand, within EX31_RS10460 at 4.023 kb on + strand, within EX31_RS10460 at 4.023 kb on + strand, within EX31_RS10460 at 4.023 kb on + strand, within EX31_RS10460 at 4.023 kb on + strand, within EX31_RS10460 at 4.024 kb on - strand, within EX31_RS10460 at 4.130 kb on - strand, within EX31_RS10460 at 4.130 kb on - strand, within EX31_RS10460 at 4.155 kb on + strand, within EX31_RS10460 at 4.155 kb on + strand, within EX31_RS10460 at 4.156 kb on - strand, within EX31_RS10460 at 4.156 kb on - strand, within EX31_RS10460 at 4.365 kb on + strand, within EX31_RS10460 at 4.525 kb on - strand, within EX31_RS10460 at 4.530 kb on + strand, within EX31_RS10460 at 4.531 kb on - strand, within EX31_RS10460 at 4.531 kb on - strand, within EX31_RS10460 at 4.557 kb on + strand, within EX31_RS10460
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 445 + +3.6 803 + EX31_RS10455 0.14 -0.4 852 - EX31_RS10455 0.16 +0.0 852 - EX31_RS10455 0.16 +0.4 852 - EX31_RS10455 0.16 +0.8 887 - EX31_RS10455 0.17 +0.6 1,013 + EX31_RS10455 0.20 -1.0 1,013 + EX31_RS10455 0.20 +0.4 1,106 - EX31_RS10455 0.23 -1.2 1,114 + EX31_RS10455 0.23 +0.9 1,115 - EX31_RS10455 0.23 -1.5 1,372 + EX31_RS10455 0.31 -0.3 1,418 + EX31_RS10455 0.32 +0.8 1,426 + EX31_RS10455 0.33 -2.9 1,426 + EX31_RS10455 0.33 -1.2 1,426 + EX31_RS10455 0.33 +0.1 1,426 + EX31_RS10455 0.33 +0.3 1,427 - EX31_RS10455 0.33 +0.6 1,427 - EX31_RS10455 0.33 +0.1 1,657 + EX31_RS10455 0.40 -0.7 1,658 - EX31_RS10455 0.40 +2.6 1,903 + EX31_RS10455 0.47 -1.2 1,903 + EX31_RS10455 0.47 -1.0 1,904 - EX31_RS10455 0.47 +1.1 1,904 - EX31_RS10455 0.47 -0.6 2,413 + EX31_RS10455 0.62 -0.2 2,605 + EX31_RS10455 0.68 +1.6 2,677 + EX31_RS10455 0.70 +0.2 2,677 + EX31_RS10455 0.70 +2.6 2,819 - EX31_RS10455 0.74 -0.8 2,935 - EX31_RS10455 0.77 -1.2 2,935 - EX31_RS10455 0.77 +1.1 2,935 - EX31_RS10455 0.77 -0.2 2,938 + EX31_RS10455 0.77 -0.7 2,938 + EX31_RS10455 0.77 -1.8 2,939 - EX31_RS10455 0.78 +0.8 3,032 + EX31_RS10455 0.80 -1.0 3,032 + EX31_RS10455 0.80 -0.4 3,032 + EX31_RS10455 0.80 +1.0 3,033 - EX31_RS10455 0.80 +0.8 3,033 - EX31_RS10455 0.80 -1.1 3,264 + EX31_RS10455 0.87 -0.4 3,486 + +1.0 3,486 + +1.9 3,487 - +0.0 3,589 + -1.7 3,717 + +0.8 3,727 + +0.3 3,727 + -0.2 3,727 + +0.9 3,728 - +0.3 3,728 - -2.3 3,735 + +0.7 3,759 + +0.8 3,872 + +0.1 3,991 + EX31_RS10460 0.15 +0.9 4,023 + EX31_RS10460 0.17 -1.0 4,023 + EX31_RS10460 0.17 -0.3 4,023 + EX31_RS10460 0.17 +2.3 4,023 + EX31_RS10460 0.17 -0.4 4,024 - EX31_RS10460 0.17 -1.2 4,130 - EX31_RS10460 0.23 -0.9 4,130 - EX31_RS10460 0.23 -0.8 4,155 + EX31_RS10460 0.24 -0.5 4,155 + EX31_RS10460 0.24 +0.4 4,156 - EX31_RS10460 0.24 -2.0 4,156 - EX31_RS10460 0.24 +1.3 4,365 + EX31_RS10460 0.37 -0.2 4,525 - EX31_RS10460 0.46 -1.3 4,530 + EX31_RS10460 0.46 -0.4 4,531 - EX31_RS10460 0.46 +1.4 4,531 - EX31_RS10460 0.46 +2.2 4,557 + EX31_RS10460 0.48 +1.9
Or see this region's nucleotide sequence