Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06000 and EX31_RS06005 are separated by 71 nucleotides EX31_RS06005 and EX31_RS06010 are separated by 100 nucleotides EX31_RS06010 and EX31_RS06015 are separated by 75 nucleotides
EX31_RS06000: EX31_RS06000 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE, at 360,026 to 361,390
_RS06000
EX31_RS06005: EX31_RS06005 - short chain dehydrogenase, at 361,462 to 362,061
_RS06005
EX31_RS06010: EX31_RS06010 - LysR family transcriptional regulator, at 362,162 to 363,049
_RS06010
EX31_RS06015: EX31_RS06015 - methyl-accepting chemotaxis protein, at 363,125 to 365,020
_RS06015
Position (kb) 362
363
364 Strain fitness (log2 ratio) -2
-1
0
1
2 at 361.168 kb on + strand, within EX31_RS06000 at 361.169 kb on - strand, within EX31_RS06000 at 361.336 kb on + strand at 361.350 kb on + strand at 361.379 kb on - strand at 361.388 kb on + strand at 361.388 kb on + strand at 361.389 kb on - strand at 361.389 kb on - strand at 361.389 kb on - strand at 361.389 kb on - strand at 361.389 kb on - strand at 361.474 kb on - strand at 361.475 kb on + strand at 361.475 kb on + strand at 361.476 kb on - strand at 361.572 kb on - strand, within EX31_RS06005 at 361.591 kb on + strand, within EX31_RS06005 at 361.591 kb on + strand, within EX31_RS06005 at 361.591 kb on + strand, within EX31_RS06005 at 361.591 kb on + strand, within EX31_RS06005 at 361.592 kb on - strand, within EX31_RS06005 at 361.592 kb on - strand, within EX31_RS06005 at 361.594 kb on + strand, within EX31_RS06005 at 361.594 kb on + strand, within EX31_RS06005 at 361.595 kb on - strand, within EX31_RS06005 at 361.678 kb on + strand, within EX31_RS06005 at 361.679 kb on - strand, within EX31_RS06005 at 361.679 kb on - strand, within EX31_RS06005 at 361.679 kb on - strand, within EX31_RS06005 at 361.807 kb on - strand, within EX31_RS06005 at 361.878 kb on + strand, within EX31_RS06005 at 361.878 kb on + strand, within EX31_RS06005 at 361.878 kb on + strand, within EX31_RS06005 at 361.879 kb on - strand, within EX31_RS06005 at 361.879 kb on - strand, within EX31_RS06005 at 361.879 kb on - strand, within EX31_RS06005 at 361.879 kb on - strand, within EX31_RS06005 at 361.879 kb on - strand, within EX31_RS06005 at 361.879 kb on - strand, within EX31_RS06005 at 361.879 kb on - strand, within EX31_RS06005 at 361.924 kb on + strand, within EX31_RS06005 at 361.924 kb on + strand, within EX31_RS06005 at 361.924 kb on + strand, within EX31_RS06005 at 361.924 kb on + strand, within EX31_RS06005 at 361.924 kb on + strand, within EX31_RS06005 at 361.925 kb on - strand, within EX31_RS06005 at 361.925 kb on - strand, within EX31_RS06005 at 361.944 kb on + strand, within EX31_RS06005 at 361.944 kb on + strand, within EX31_RS06005 at 362.001 kb on + strand, within EX31_RS06005 at 362.031 kb on + strand at 362.031 kb on + strand at 362.031 kb on + strand at 362.031 kb on + strand at 362.031 kb on + strand at 362.031 kb on + strand at 362.031 kb on + strand at 362.031 kb on + strand at 362.031 kb on + strand at 362.031 kb on + strand at 362.031 kb on + strand at 362.031 kb on + strand at 362.031 kb on + strand at 362.032 kb on - strand at 362.032 kb on - strand at 362.032 kb on - strand at 362.032 kb on - strand at 362.032 kb on - strand at 362.032 kb on - strand at 362.032 kb on - strand at 362.032 kb on - strand at 362.032 kb on - strand at 362.066 kb on + strand at 362.066 kb on + strand at 362.067 kb on - strand at 362.067 kb on - strand at 362.067 kb on - strand at 362.067 kb on - strand at 362.067 kb on - strand at 362.113 kb on + strand at 362.114 kb on - strand at 362.117 kb on + strand at 362.117 kb on + strand at 362.118 kb on - strand at 362.119 kb on + strand at 362.119 kb on + strand at 362.120 kb on - strand at 362.120 kb on - strand at 362.127 kb on + strand at 362.127 kb on + strand at 362.127 kb on + strand at 362.158 kb on + strand at 362.158 kb on + strand at 362.158 kb on + strand at 362.159 kb on - strand at 362.159 kb on - strand at 362.167 kb on + strand at 362.200 kb on - strand at 362.357 kb on + strand, within EX31_RS06010 at 362.358 kb on - strand, within EX31_RS06010 at 362.501 kb on + strand, within EX31_RS06010 at 362.501 kb on + strand, within EX31_RS06010 at 362.523 kb on + strand, within EX31_RS06010 at 362.524 kb on - strand, within EX31_RS06010 at 362.524 kb on - strand, within EX31_RS06010 at 362.539 kb on - strand, within EX31_RS06010 at 362.642 kb on + strand, within EX31_RS06010 at 362.672 kb on + strand, within EX31_RS06010 at 362.673 kb on - strand, within EX31_RS06010 at 362.676 kb on + strand, within EX31_RS06010 at 362.677 kb on - strand, within EX31_RS06010 at 362.677 kb on - strand, within EX31_RS06010 at 362.677 kb on - strand, within EX31_RS06010 at 362.677 kb on - strand, within EX31_RS06010 at 362.737 kb on + strand, within EX31_RS06010 at 362.738 kb on - strand, within EX31_RS06010 at 362.816 kb on + strand, within EX31_RS06010 at 362.816 kb on + strand, within EX31_RS06010 at 362.817 kb on - strand, within EX31_RS06010 at 362.817 kb on - strand, within EX31_RS06010 at 362.817 kb on - strand, within EX31_RS06010 at 362.817 kb on - strand, within EX31_RS06010 at 362.817 kb on - strand, within EX31_RS06010 at 362.944 kb on + strand, within EX31_RS06010 at 362.975 kb on + strand at 363.019 kb on + strand at 363.019 kb on + strand at 363.020 kb on - strand at 363.045 kb on - strand at 363.045 kb on - strand at 363.055 kb on - strand at 363.133 kb on + strand at 363.133 kb on + strand at 363.133 kb on + strand at 363.133 kb on + strand at 363.133 kb on + strand at 363.134 kb on - strand at 363.143 kb on + strand at 363.143 kb on + strand at 363.143 kb on + strand at 363.260 kb on + strand at 363.261 kb on - strand at 363.261 kb on - strand at 363.261 kb on - strand at 363.261 kb on - strand at 363.601 kb on + strand, within EX31_RS06015 at 363.851 kb on - strand, within EX31_RS06015 at 363.969 kb on - strand, within EX31_RS06015 at 363.969 kb on - strand, within EX31_RS06015 at 364.006 kb on + strand, within EX31_RS06015
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 361,168 + EX31_RS06000 0.84 -0.1 361,169 - EX31_RS06000 0.84 -0.3 361,336 + -0.3 361,350 + -1.6 361,379 - -0.5 361,388 + -0.2 361,388 + +0.2 361,389 - +0.6 361,389 - -0.7 361,389 - -0.5 361,389 - +0.4 361,389 - -0.4 361,474 - +0.0 361,475 + +0.1 361,475 + -0.0 361,476 - -0.1 361,572 - EX31_RS06005 0.18 -0.1 361,591 + EX31_RS06005 0.21 +0.4 361,591 + EX31_RS06005 0.21 +0.9 361,591 + EX31_RS06005 0.21 -0.6 361,591 + EX31_RS06005 0.21 +0.5 361,592 - EX31_RS06005 0.22 +0.7 361,592 - EX31_RS06005 0.22 +0.6 361,594 + EX31_RS06005 0.22 +1.1 361,594 + EX31_RS06005 0.22 +0.7 361,595 - EX31_RS06005 0.22 +0.0 361,678 + EX31_RS06005 0.36 +0.5 361,679 - EX31_RS06005 0.36 -0.2 361,679 - EX31_RS06005 0.36 -0.2 361,679 - EX31_RS06005 0.36 -0.9 361,807 - EX31_RS06005 0.57 +0.1 361,878 + EX31_RS06005 0.69 -0.3 361,878 + EX31_RS06005 0.69 -0.2 361,878 + EX31_RS06005 0.69 +0.7 361,879 - EX31_RS06005 0.69 +0.5 361,879 - EX31_RS06005 0.69 +0.2 361,879 - EX31_RS06005 0.69 +0.7 361,879 - EX31_RS06005 0.69 +0.2 361,879 - EX31_RS06005 0.69 -0.3 361,879 - EX31_RS06005 0.69 +0.8 361,879 - EX31_RS06005 0.69 -0.9 361,924 + EX31_RS06005 0.77 -0.2 361,924 + EX31_RS06005 0.77 -1.5 361,924 + EX31_RS06005 0.77 +0.9 361,924 + EX31_RS06005 0.77 -0.3 361,924 + EX31_RS06005 0.77 +0.7 361,925 - EX31_RS06005 0.77 +0.3 361,925 - EX31_RS06005 0.77 -1.4 361,944 + EX31_RS06005 0.80 -0.0 361,944 + EX31_RS06005 0.80 +2.3 362,001 + EX31_RS06005 0.90 -0.2 362,031 + +0.3 362,031 + -1.7 362,031 + +0.1 362,031 + +0.6 362,031 + +0.2 362,031 + -0.0 362,031 + +0.1 362,031 + +0.3 362,031 + +0.1 362,031 + -1.0 362,031 + -0.6 362,031 + +0.9 362,031 + +0.2 362,032 - -0.1 362,032 - -0.5 362,032 - -0.2 362,032 - +0.2 362,032 - +0.4 362,032 - +0.6 362,032 - +0.1 362,032 - -1.0 362,032 - -0.4 362,066 + +2.2 362,066 + +0.6 362,067 - +0.1 362,067 - +0.3 362,067 - +0.6 362,067 - +0.6 362,067 - +0.0 362,113 + +1.4 362,114 - -0.2 362,117 + +0.6 362,117 + +0.4 362,118 - +0.2 362,119 + +0.5 362,119 + +1.4 362,120 - -1.8 362,120 - +0.6 362,127 + +1.4 362,127 + +1.2 362,127 + -0.0 362,158 + -1.3 362,158 + -0.5 362,158 + -0.4 362,159 - -1.7 362,159 - +0.3 362,167 + +0.5 362,200 - -1.3 362,357 + EX31_RS06010 0.22 -0.1 362,358 - EX31_RS06010 0.22 -0.6 362,501 + EX31_RS06010 0.38 -0.8 362,501 + EX31_RS06010 0.38 -0.0 362,523 + EX31_RS06010 0.41 -0.3 362,524 - EX31_RS06010 0.41 +0.5 362,524 - EX31_RS06010 0.41 +0.1 362,539 - EX31_RS06010 0.42 +0.9 362,642 + EX31_RS06010 0.54 -0.3 362,672 + EX31_RS06010 0.57 -1.0 362,673 - EX31_RS06010 0.58 -0.0 362,676 + EX31_RS06010 0.58 +0.6 362,677 - EX31_RS06010 0.58 -0.5 362,677 - EX31_RS06010 0.58 -0.0 362,677 - EX31_RS06010 0.58 -0.5 362,677 - EX31_RS06010 0.58 -0.3 362,737 + EX31_RS06010 0.65 -0.1 362,738 - EX31_RS06010 0.65 -1.4 362,816 + EX31_RS06010 0.74 +0.7 362,816 + EX31_RS06010 0.74 +1.5 362,817 - EX31_RS06010 0.74 +0.1 362,817 - EX31_RS06010 0.74 +0.8 362,817 - EX31_RS06010 0.74 -0.7 362,817 - EX31_RS06010 0.74 +0.7 362,817 - EX31_RS06010 0.74 -0.4 362,944 + EX31_RS06010 0.88 +0.6 362,975 + +0.8 363,019 + -0.9 363,019 + -0.1 363,020 - -0.4 363,045 - -0.6 363,045 - +0.5 363,055 - -0.1 363,133 + -0.4 363,133 + -0.4 363,133 + +1.1 363,133 + +0.4 363,133 + -0.1 363,134 - +0.5 363,143 + -0.2 363,143 + -1.4 363,143 + +1.9 363,260 + -0.3 363,261 - +0.2 363,261 - +0.6 363,261 - -0.6 363,261 - -0.4 363,601 + EX31_RS06015 0.25 -0.2 363,851 - EX31_RS06015 0.38 -0.8 363,969 - EX31_RS06015 0.45 +0.0 363,969 - EX31_RS06015 0.45 -0.8 364,006 + EX31_RS06015 0.46 +0.1
Or see this region's nucleotide sequence