Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01830 and EX31_RS01835 are separated by 146 nucleotides EX31_RS01835 and EX31_RS01840 are separated by 405 nucleotides
EX31_RS01830: EX31_RS01830 - 5-histidylcysteine sulfoxide synthase, at 392,276 to 394,417
_RS01830
EX31_RS01835: EX31_RS01835 - site-specific integrase, at 394,564 to 395,688
_RS01835
EX31_RS01840: EX31_RS01840 - PAS domain-containing protein, at 396,094 to 397,641
_RS01840
Position (kb) 394
395
396 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 393.650 kb on - strand, within EX31_RS01830 at 393.686 kb on - strand, within EX31_RS01830 at 393.727 kb on - strand, within EX31_RS01830 at 393.748 kb on + strand, within EX31_RS01830 at 393.748 kb on + strand, within EX31_RS01830 at 393.749 kb on - strand, within EX31_RS01830 at 393.796 kb on + strand, within EX31_RS01830 at 393.797 kb on - strand, within EX31_RS01830 at 393.802 kb on + strand, within EX31_RS01830 at 393.897 kb on - strand, within EX31_RS01830 at 393.897 kb on - strand, within EX31_RS01830 at 393.897 kb on - strand, within EX31_RS01830 at 394.000 kb on + strand, within EX31_RS01830 at 394.001 kb on - strand, within EX31_RS01830 at 394.023 kb on - strand, within EX31_RS01830 at 394.023 kb on - strand, within EX31_RS01830 at 394.067 kb on - strand, within EX31_RS01830 at 394.067 kb on - strand, within EX31_RS01830 at 394.069 kb on - strand, within EX31_RS01830 at 394.069 kb on - strand, within EX31_RS01830 at 394.178 kb on - strand, within EX31_RS01830 at 394.180 kb on + strand, within EX31_RS01830 at 394.181 kb on - strand, within EX31_RS01830 at 394.315 kb on - strand at 394.422 kb on - strand at 394.463 kb on + strand at 394.512 kb on - strand at 394.514 kb on - strand at 394.514 kb on - strand at 394.557 kb on - strand at 394.669 kb on - strand at 394.669 kb on - strand at 394.669 kb on - strand at 394.728 kb on - strand, within EX31_RS01835 at 394.728 kb on - strand, within EX31_RS01835 at 394.728 kb on - strand, within EX31_RS01835 at 394.761 kb on + strand, within EX31_RS01835 at 394.839 kb on + strand, within EX31_RS01835 at 394.839 kb on + strand, within EX31_RS01835 at 394.840 kb on - strand, within EX31_RS01835 at 394.873 kb on + strand, within EX31_RS01835 at 394.874 kb on - strand, within EX31_RS01835 at 394.885 kb on + strand, within EX31_RS01835 at 394.976 kb on - strand, within EX31_RS01835 at 395.041 kb on - strand, within EX31_RS01835 at 395.275 kb on - strand, within EX31_RS01835 at 395.278 kb on + strand, within EX31_RS01835 at 395.278 kb on + strand, within EX31_RS01835 at 395.279 kb on - strand, within EX31_RS01835 at 395.279 kb on - strand, within EX31_RS01835 at 395.298 kb on - strand, within EX31_RS01835 at 395.298 kb on - strand, within EX31_RS01835 at 395.332 kb on - strand, within EX31_RS01835 at 395.332 kb on - strand, within EX31_RS01835 at 395.332 kb on - strand, within EX31_RS01835 at 395.332 kb on - strand, within EX31_RS01835 at 395.383 kb on + strand, within EX31_RS01835 at 395.389 kb on - strand, within EX31_RS01835 at 395.389 kb on - strand, within EX31_RS01835 at 395.527 kb on - strand, within EX31_RS01835 at 395.613 kb on - strand at 395.663 kb on - strand at 395.683 kb on - strand at 395.683 kb on - strand at 395.683 kb on - strand at 395.724 kb on + strand at 395.724 kb on + strand at 395.726 kb on + strand at 395.726 kb on + strand at 395.726 kb on + strand at 395.727 kb on - strand at 395.727 kb on - strand at 395.745 kb on - strand at 395.771 kb on + strand at 395.772 kb on - strand at 395.774 kb on + strand at 395.798 kb on + strand at 395.799 kb on - strand at 395.821 kb on - strand at 395.834 kb on - strand at 395.853 kb on - strand at 395.853 kb on - strand at 395.884 kb on + strand at 395.884 kb on + strand at 395.886 kb on + strand at 395.887 kb on - strand at 395.887 kb on - strand at 395.893 kb on + strand at 395.906 kb on + strand at 395.906 kb on + strand at 395.907 kb on - strand at 395.917 kb on + strand at 395.917 kb on + strand at 395.917 kb on + strand at 395.917 kb on + strand at 395.918 kb on - strand at 395.918 kb on - strand at 395.918 kb on - strand at 395.918 kb on - strand at 395.918 kb on - strand at 395.918 kb on - strand at 395.918 kb on - strand at 395.992 kb on - strand at 396.043 kb on + strand at 396.093 kb on + strand at 396.180 kb on + strand at 396.183 kb on + strand at 396.184 kb on - strand at 396.184 kb on - strand at 396.184 kb on - strand at 396.187 kb on + strand at 396.205 kb on - strand at 396.205 kb on - strand at 396.205 kb on - strand at 396.207 kb on - strand at 396.235 kb on - strand at 396.235 kb on - strand at 396.235 kb on - strand at 396.298 kb on - strand, within EX31_RS01840 at 396.306 kb on + strand, within EX31_RS01840 at 396.311 kb on + strand, within EX31_RS01840 at 396.311 kb on + strand, within EX31_RS01840 at 396.311 kb on + strand, within EX31_RS01840 at 396.311 kb on + strand, within EX31_RS01840 at 396.620 kb on + strand, within EX31_RS01840
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 393,650 - EX31_RS01830 0.64 +0.2 393,686 - EX31_RS01830 0.66 +1.6 393,727 - EX31_RS01830 0.68 +1.0 393,748 + EX31_RS01830 0.69 -0.3 393,748 + EX31_RS01830 0.69 +0.3 393,749 - EX31_RS01830 0.69 +0.8 393,796 + EX31_RS01830 0.71 -0.2 393,797 - EX31_RS01830 0.71 -0.5 393,802 + EX31_RS01830 0.71 -0.9 393,897 - EX31_RS01830 0.76 +1.0 393,897 - EX31_RS01830 0.76 +1.5 393,897 - EX31_RS01830 0.76 -0.4 394,000 + EX31_RS01830 0.80 +2.4 394,001 - EX31_RS01830 0.81 +0.7 394,023 - EX31_RS01830 0.82 +0.6 394,023 - EX31_RS01830 0.82 +1.1 394,067 - EX31_RS01830 0.84 -0.8 394,067 - EX31_RS01830 0.84 +1.0 394,069 - EX31_RS01830 0.84 +0.7 394,069 - EX31_RS01830 0.84 -1.2 394,178 - EX31_RS01830 0.89 +0.0 394,180 + EX31_RS01830 0.89 -0.8 394,181 - EX31_RS01830 0.89 -0.3 394,315 - +0.8 394,422 - +0.2 394,463 + +0.6 394,512 - -0.1 394,514 - -0.2 394,514 - -0.4 394,557 - -0.4 394,669 - +0.0 394,669 - +0.2 394,669 - +0.6 394,728 - EX31_RS01835 0.15 -0.2 394,728 - EX31_RS01835 0.15 +0.0 394,728 - EX31_RS01835 0.15 +1.2 394,761 + EX31_RS01835 0.18 -0.5 394,839 + EX31_RS01835 0.24 +0.9 394,839 + EX31_RS01835 0.24 -0.9 394,840 - EX31_RS01835 0.25 -0.3 394,873 + EX31_RS01835 0.27 +1.1 394,874 - EX31_RS01835 0.28 +0.4 394,885 + EX31_RS01835 0.29 -0.5 394,976 - EX31_RS01835 0.37 -0.1 395,041 - EX31_RS01835 0.42 +1.0 395,275 - EX31_RS01835 0.63 -1.2 395,278 + EX31_RS01835 0.63 +1.2 395,278 + EX31_RS01835 0.63 +0.6 395,279 - EX31_RS01835 0.64 -1.3 395,279 - EX31_RS01835 0.64 -0.5 395,298 - EX31_RS01835 0.65 +0.3 395,298 - EX31_RS01835 0.65 +0.1 395,332 - EX31_RS01835 0.68 -0.4 395,332 - EX31_RS01835 0.68 +0.5 395,332 - EX31_RS01835 0.68 +0.8 395,332 - EX31_RS01835 0.68 -0.1 395,383 + EX31_RS01835 0.73 -0.5 395,389 - EX31_RS01835 0.73 -0.4 395,389 - EX31_RS01835 0.73 +0.8 395,527 - EX31_RS01835 0.86 +0.0 395,613 - +0.2 395,663 - +0.6 395,683 - +0.2 395,683 - -0.7 395,683 - -0.7 395,724 + +0.8 395,724 + -1.4 395,726 + +1.6 395,726 + +0.3 395,726 + -0.6 395,727 - +0.7 395,727 - -0.9 395,745 - +2.5 395,771 + +0.6 395,772 - +1.1 395,774 + -0.7 395,798 + +0.7 395,799 - +0.8 395,821 - +0.8 395,834 - +1.1 395,853 - -0.5 395,853 - -1.9 395,884 + +0.0 395,884 + -0.5 395,886 + -0.7 395,887 - -0.0 395,887 - -0.4 395,893 + +0.1 395,906 + +0.0 395,906 + +0.2 395,907 - +0.8 395,917 + +0.4 395,917 + -0.0 395,917 + -1.5 395,917 + +0.6 395,918 - +0.2 395,918 - -0.5 395,918 - -0.9 395,918 - -0.4 395,918 - +3.0 395,918 - -0.5 395,918 - +0.5 395,992 - -1.9 396,043 + +0.8 396,093 + +1.6 396,180 + +0.6 396,183 + +1.3 396,184 - +0.4 396,184 - +0.8 396,184 - +0.8 396,187 + +0.2 396,205 - +0.9 396,205 - -0.6 396,205 - +0.7 396,207 - +0.9 396,235 - -0.4 396,235 - +0.4 396,235 - -1.7 396,298 - EX31_RS01840 0.13 +1.6 396,306 + EX31_RS01840 0.14 -0.0 396,311 + EX31_RS01840 0.14 +0.2 396,311 + EX31_RS01840 0.14 +0.5 396,311 + EX31_RS01840 0.14 +0.2 396,311 + EX31_RS01840 0.14 +2.3 396,620 + EX31_RS01840 0.34 -0.8
Or see this region's nucleotide sequence