Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15420 and EX31_RS15425 are separated by 311 nucleotides EX31_RS15425 and EX31_RS25400 are separated by 246 nucleotides EX31_RS25400 and EX31_RS15430 are separated by 298 nucleotides
EX31_RS15420: EX31_RS15420 - pyruvate:ferredoxin (flavodoxin) oxidoreductase, at 35,316 to 38,834
_RS15420
EX31_RS15425: EX31_RS15425 - DUF2461 family protein, at 39,146 to 39,364
_RS15425
EX31_RS25400: EX31_RS25400 - transcriptional regulator, at 39,611 to 39,727
_RS25400
EX31_RS15430: EX31_RS15430 - FAD-binding oxidoreductase, at 40,026 to 41,126
_RS15430
Position (kb) 39
40 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 38.622 kb on + strand at 38.622 kb on + strand at 38.623 kb on - strand at 38.667 kb on + strand at 38.744 kb on + strand at 38.744 kb on + strand at 38.744 kb on + strand at 38.744 kb on + strand at 38.745 kb on - strand at 38.745 kb on - strand at 38.745 kb on - strand at 38.745 kb on - strand at 38.745 kb on - strand at 38.747 kb on + strand at 38.748 kb on - strand at 38.752 kb on + strand at 38.779 kb on + strand at 38.779 kb on + strand at 38.779 kb on + strand at 38.779 kb on + strand at 38.779 kb on + strand at 38.780 kb on - strand at 38.788 kb on + strand at 38.788 kb on + strand at 38.788 kb on + strand at 38.788 kb on + strand at 38.788 kb on + strand at 38.788 kb on + strand at 38.788 kb on + strand at 38.788 kb on + strand at 38.789 kb on - strand at 38.789 kb on - strand at 38.789 kb on - strand at 38.789 kb on - strand at 38.789 kb on - strand at 38.789 kb on - strand at 38.789 kb on - strand at 38.789 kb on - strand at 38.789 kb on - strand at 38.789 kb on - strand at 38.789 kb on - strand at 38.789 kb on - strand at 38.789 kb on - strand at 38.789 kb on - strand at 38.789 kb on - strand at 39.012 kb on + strand at 39.038 kb on - strand at 39.045 kb on + strand at 39.045 kb on + strand at 39.046 kb on - strand at 39.046 kb on - strand at 39.046 kb on - strand at 39.068 kb on + strand at 39.068 kb on + strand at 39.068 kb on + strand at 39.068 kb on + strand at 39.068 kb on + strand at 39.069 kb on - strand at 39.069 kb on - strand at 39.069 kb on - strand at 39.071 kb on + strand at 39.071 kb on + strand at 39.177 kb on + strand, within EX31_RS15425 at 39.177 kb on + strand, within EX31_RS15425 at 39.177 kb on + strand, within EX31_RS15425 at 39.177 kb on + strand, within EX31_RS15425 at 39.177 kb on + strand, within EX31_RS15425 at 39.178 kb on - strand, within EX31_RS15425 at 39.178 kb on - strand, within EX31_RS15425 at 39.178 kb on - strand, within EX31_RS15425 at 39.275 kb on - strand, within EX31_RS15425 at 39.275 kb on - strand, within EX31_RS15425 at 39.290 kb on + strand, within EX31_RS15425 at 39.300 kb on - strand, within EX31_RS15425 at 39.399 kb on + strand at 39.475 kb on + strand at 39.504 kb on + strand at 39.504 kb on + strand at 39.504 kb on + strand at 39.504 kb on + strand at 39.528 kb on + strand at 39.528 kb on + strand at 39.528 kb on + strand at 39.528 kb on + strand at 39.528 kb on + strand at 39.529 kb on - strand at 39.529 kb on - strand at 39.529 kb on - strand at 39.529 kb on - strand at 39.529 kb on - strand at 39.529 kb on - strand at 39.529 kb on - strand at 39.529 kb on - strand at 39.542 kb on + strand at 39.542 kb on + strand at 39.542 kb on + strand at 39.543 kb on - strand at 39.543 kb on - strand at 39.543 kb on - strand at 39.543 kb on - strand at 39.543 kb on - strand at 39.543 kb on - strand at 39.550 kb on - strand at 39.550 kb on - strand at 39.563 kb on + strand at 39.563 kb on + strand at 39.567 kb on + strand at 39.567 kb on + strand at 39.568 kb on - strand at 39.568 kb on - strand at 39.578 kb on + strand at 39.596 kb on + strand at 39.596 kb on + strand at 39.596 kb on + strand at 39.597 kb on - strand at 39.597 kb on - strand at 39.613 kb on - strand at 39.613 kb on - strand at 39.621 kb on + strand at 39.621 kb on + strand at 39.621 kb on + strand at 39.621 kb on + strand at 39.622 kb on - strand at 39.622 kb on - strand at 39.622 kb on - strand at 39.653 kb on - strand, within EX31_RS25400 at 39.653 kb on - strand, within EX31_RS25400 at 39.710 kb on + strand, within EX31_RS25400 at 39.711 kb on - strand, within EX31_RS25400 at 39.711 kb on - strand, within EX31_RS25400 at 39.744 kb on + strand at 39.745 kb on - strand at 39.812 kb on + strand at 39.812 kb on + strand at 39.812 kb on + strand at 39.838 kb on + strand at 39.839 kb on - strand at 39.859 kb on - strand at 39.859 kb on - strand at 39.880 kb on + strand at 39.880 kb on + strand at 39.880 kb on + strand at 39.880 kb on + strand at 39.880 kb on + strand at 39.881 kb on - strand at 39.881 kb on - strand at 39.881 kb on - strand at 39.881 kb on - strand at 39.881 kb on - strand at 39.881 kb on - strand at 39.888 kb on + strand at 39.888 kb on + strand at 39.888 kb on + strand at 39.889 kb on - strand at 39.889 kb on - strand at 39.890 kb on + strand at 39.890 kb on + strand at 39.891 kb on - strand at 39.891 kb on - strand at 39.914 kb on + strand at 39.915 kb on - strand at 39.915 kb on - strand at 39.915 kb on - strand at 39.915 kb on - strand at 39.922 kb on + strand at 39.922 kb on + strand at 39.922 kb on + strand at 39.922 kb on + strand at 39.922 kb on + strand at 39.923 kb on - strand at 39.923 kb on - strand at 39.923 kb on - strand at 39.923 kb on - strand at 39.923 kb on - strand at 39.923 kb on - strand at 39.926 kb on + strand at 39.926 kb on + strand at 39.926 kb on + strand at 39.926 kb on + strand at 39.926 kb on + strand at 39.926 kb on + strand at 39.926 kb on + strand at 39.927 kb on - strand at 39.941 kb on + strand at 39.966 kb on + strand at 39.972 kb on + strand at 39.978 kb on + strand at 39.999 kb on + strand at 39.999 kb on + strand at 40.021 kb on + strand at 40.021 kb on + strand at 40.022 kb on - strand at 40.022 kb on - strand at 40.022 kb on - strand at 40.061 kb on + strand at 40.061 kb on + strand at 40.061 kb on + strand at 40.061 kb on + strand at 40.061 kb on + strand at 40.061 kb on + strand at 40.062 kb on - strand at 40.094 kb on + strand at 40.191 kb on + strand, within EX31_RS15430 at 40.191 kb on + strand, within EX31_RS15430 at 40.191 kb on + strand, within EX31_RS15430 at 40.191 kb on + strand, within EX31_RS15430 at 40.194 kb on + strand, within EX31_RS15430 at 40.194 kb on + strand, within EX31_RS15430 at 40.194 kb on + strand, within EX31_RS15430 at 40.194 kb on + strand, within EX31_RS15430 at 40.194 kb on + strand, within EX31_RS15430 at 40.195 kb on - strand, within EX31_RS15430 at 40.195 kb on - strand, within EX31_RS15430 at 40.195 kb on - strand, within EX31_RS15430 at 40.195 kb on - strand, within EX31_RS15430 at 40.196 kb on + strand, within EX31_RS15430 at 40.196 kb on + strand, within EX31_RS15430 at 40.196 kb on + strand, within EX31_RS15430 at 40.196 kb on + strand, within EX31_RS15430 at 40.197 kb on - strand, within EX31_RS15430 at 40.197 kb on - strand, within EX31_RS15430 at 40.197 kb on - strand, within EX31_RS15430 at 40.197 kb on - strand, within EX31_RS15430 at 40.197 kb on - strand, within EX31_RS15430 at 40.197 kb on - strand, within EX31_RS15430 at 40.236 kb on + strand, within EX31_RS15430 at 40.237 kb on - strand, within EX31_RS15430 at 40.237 kb on - strand, within EX31_RS15430 at 40.241 kb on + strand, within EX31_RS15430 at 40.241 kb on + strand, within EX31_RS15430 at 40.265 kb on - strand, within EX31_RS15430 at 40.319 kb on + strand, within EX31_RS15430 at 40.319 kb on + strand, within EX31_RS15430 at 40.320 kb on - strand, within EX31_RS15430 at 40.320 kb on - strand, within EX31_RS15430 at 40.320 kb on - strand, within EX31_RS15430 at 40.341 kb on + strand, within EX31_RS15430 at 40.342 kb on - strand, within EX31_RS15430 at 40.376 kb on + strand, within EX31_RS15430 at 40.377 kb on - strand, within EX31_RS15430 at 40.380 kb on - strand, within EX31_RS15430 at 40.391 kb on + strand, within EX31_RS15430 at 40.391 kb on + strand, within EX31_RS15430 at 40.425 kb on + strand, within EX31_RS15430 at 40.425 kb on + strand, within EX31_RS15430 at 40.425 kb on + strand, within EX31_RS15430 at 40.425 kb on + strand, within EX31_RS15430 at 40.426 kb on - strand, within EX31_RS15430 at 40.426 kb on - strand, within EX31_RS15430 at 40.427 kb on + strand, within EX31_RS15430 at 40.427 kb on + strand, within EX31_RS15430 at 40.428 kb on - strand, within EX31_RS15430 at 40.428 kb on - strand, within EX31_RS15430 at 40.428 kb on - strand, within EX31_RS15430 at 40.487 kb on + strand, within EX31_RS15430 at 40.488 kb on - strand, within EX31_RS15430 at 40.488 kb on - strand, within EX31_RS15430 at 40.553 kb on + strand, within EX31_RS15430 at 40.554 kb on - strand, within EX31_RS15430 at 40.554 kb on - strand, within EX31_RS15430 at 40.554 kb on - strand, within EX31_RS15430 at 40.559 kb on + strand, within EX31_RS15430 at 40.559 kb on + strand, within EX31_RS15430 at 40.560 kb on - strand, within EX31_RS15430 at 40.590 kb on + strand, within EX31_RS15430 at 40.590 kb on + strand, within EX31_RS15430 at 40.590 kb on + strand, within EX31_RS15430 at 40.590 kb on + strand, within EX31_RS15430 at 40.591 kb on - strand, within EX31_RS15430 at 40.591 kb on - strand, within EX31_RS15430 at 40.591 kb on - strand, within EX31_RS15430 at 40.591 kb on - strand, within EX31_RS15430 at 40.657 kb on + strand, within EX31_RS15430 at 40.657 kb on + strand, within EX31_RS15430 at 40.657 kb on + strand, within EX31_RS15430 at 40.658 kb on - strand, within EX31_RS15430 at 40.658 kb on - strand, within EX31_RS15430 at 40.658 kb on - strand, within EX31_RS15430 at 40.658 kb on - strand, within EX31_RS15430
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 38,622 + +0.2 38,622 + +0.4 38,623 - +1.5 38,667 + -0.5 38,744 + +2.5 38,744 + +1.3 38,744 + -0.5 38,744 + -0.1 38,745 - -1.2 38,745 - -0.2 38,745 - -0.3 38,745 - +0.8 38,745 - +0.7 38,747 + +0.1 38,748 - +0.4 38,752 + +1.7 38,779 + +1.3 38,779 + -0.3 38,779 + +0.2 38,779 + +0.4 38,779 + -0.2 38,780 - -2.2 38,788 + -0.8 38,788 + +0.6 38,788 + -1.5 38,788 + -0.9 38,788 + +1.1 38,788 + +0.3 38,788 + +0.5 38,788 + +0.5 38,789 - -0.5 38,789 - -1.5 38,789 - +0.1 38,789 - +0.3 38,789 - +0.0 38,789 - +0.1 38,789 - -0.4 38,789 - +3.1 38,789 - +0.5 38,789 - +1.6 38,789 - +0.8 38,789 - -0.9 38,789 - -0.6 38,789 - +0.8 38,789 - +1.5 39,012 + -1.3 39,038 - +0.1 39,045 + -1.3 39,045 + -0.6 39,046 - +0.7 39,046 - -0.8 39,046 - +1.1 39,068 + -0.9 39,068 + -0.8 39,068 + +0.2 39,068 + +0.3 39,068 + -0.2 39,069 - +0.8 39,069 - -0.0 39,069 - +0.9 39,071 + +0.9 39,071 + +0.4 39,177 + EX31_RS15425 0.14 -1.1 39,177 + EX31_RS15425 0.14 +1.0 39,177 + EX31_RS15425 0.14 +2.1 39,177 + EX31_RS15425 0.14 -0.3 39,177 + EX31_RS15425 0.14 +0.5 39,178 - EX31_RS15425 0.15 +0.6 39,178 - EX31_RS15425 0.15 -0.5 39,178 - EX31_RS15425 0.15 +1.1 39,275 - EX31_RS15425 0.59 -0.4 39,275 - EX31_RS15425 0.59 +0.5 39,290 + EX31_RS15425 0.66 -2.1 39,300 - EX31_RS15425 0.70 -0.2 39,399 + +0.8 39,475 + +0.7 39,504 + +0.7 39,504 + -0.4 39,504 + +1.5 39,504 + +0.8 39,528 + +1.4 39,528 + -0.5 39,528 + +0.2 39,528 + +0.5 39,528 + +1.2 39,529 - +0.1 39,529 - -0.3 39,529 - -1.5 39,529 - +0.2 39,529 - +0.9 39,529 - -1.0 39,529 - +0.4 39,529 - +2.3 39,542 + +1.5 39,542 + -0.5 39,542 + -0.7 39,543 - +0.2 39,543 - +1.7 39,543 - -0.4 39,543 - +0.5 39,543 - +4.1 39,543 - +0.0 39,550 - -0.2 39,550 - -1.4 39,563 + +2.7 39,563 + +0.5 39,567 + -2.1 39,567 + -0.1 39,568 - +0.1 39,568 - +1.6 39,578 + +0.7 39,596 + +0.5 39,596 + +1.2 39,596 + -0.6 39,597 - +1.9 39,597 - -0.6 39,613 - -0.5 39,613 - +0.8 39,621 + -0.7 39,621 + +1.0 39,621 + +1.1 39,621 + -0.7 39,622 - -0.4 39,622 - +0.5 39,622 - +0.8 39,653 - EX31_RS25400 0.36 -1.5 39,653 - EX31_RS25400 0.36 -0.3 39,710 + EX31_RS25400 0.85 -0.2 39,711 - EX31_RS25400 0.85 -0.3 39,711 - EX31_RS25400 0.85 +0.5 39,744 + -1.1 39,745 - +1.7 39,812 + -0.2 39,812 + +0.5 39,812 + +0.3 39,838 + -0.2 39,839 - +1.0 39,859 - -0.2 39,859 - -0.0 39,880 + +0.4 39,880 + +0.5 39,880 + -0.2 39,880 + -0.5 39,880 + +0.1 39,881 - -0.8 39,881 - -1.5 39,881 - +0.8 39,881 - +1.1 39,881 - +0.7 39,881 - +0.1 39,888 + +0.2 39,888 + -0.9 39,888 + +0.3 39,889 - +0.2 39,889 - +1.1 39,890 + +0.4 39,890 + +0.9 39,891 - +0.4 39,891 - -0.3 39,914 + +0.5 39,915 - +1.7 39,915 - +1.3 39,915 - -0.5 39,915 - +0.5 39,922 + +0.1 39,922 + +0.2 39,922 + +0.5 39,922 + +1.3 39,922 + -2.0 39,923 - +0.3 39,923 - +1.2 39,923 - -0.9 39,923 - +0.3 39,923 - -1.1 39,923 - +0.8 39,926 + +0.2 39,926 + -0.6 39,926 + +0.1 39,926 + -0.9 39,926 + -1.9 39,926 + -0.3 39,926 + -1.4 39,927 - +0.9 39,941 + +0.3 39,966 + +0.3 39,972 + -0.2 39,978 + +1.6 39,999 + +0.4 39,999 + -0.5 40,021 + -0.8 40,021 + -0.7 40,022 - +1.4 40,022 - +0.4 40,022 - +0.5 40,061 + -1.7 40,061 + +0.6 40,061 + +2.9 40,061 + -0.9 40,061 + -0.5 40,061 + -0.1 40,062 - -0.4 40,094 + -0.0 40,191 + EX31_RS15430 0.15 +0.8 40,191 + EX31_RS15430 0.15 -0.2 40,191 + EX31_RS15430 0.15 -0.1 40,191 + EX31_RS15430 0.15 -0.6 40,194 + EX31_RS15430 0.15 +0.1 40,194 + EX31_RS15430 0.15 -1.1 40,194 + EX31_RS15430 0.15 +0.1 40,194 + EX31_RS15430 0.15 +0.8 40,194 + EX31_RS15430 0.15 +0.4 40,195 - EX31_RS15430 0.15 -1.0 40,195 - EX31_RS15430 0.15 +1.2 40,195 - EX31_RS15430 0.15 +2.2 40,195 - EX31_RS15430 0.15 -0.7 40,196 + EX31_RS15430 0.15 -0.7 40,196 + EX31_RS15430 0.15 +0.6 40,196 + EX31_RS15430 0.15 +1.9 40,196 + EX31_RS15430 0.15 -0.3 40,197 - EX31_RS15430 0.16 -0.2 40,197 - EX31_RS15430 0.16 +0.3 40,197 - EX31_RS15430 0.16 +0.2 40,197 - EX31_RS15430 0.16 -0.3 40,197 - EX31_RS15430 0.16 +0.6 40,197 - EX31_RS15430 0.16 -0.6 40,236 + EX31_RS15430 0.19 -0.8 40,237 - EX31_RS15430 0.19 +2.1 40,237 - EX31_RS15430 0.19 +0.2 40,241 + EX31_RS15430 0.20 -0.8 40,241 + EX31_RS15430 0.20 +0.9 40,265 - EX31_RS15430 0.22 -0.7 40,319 + EX31_RS15430 0.27 +0.6 40,319 + EX31_RS15430 0.27 +0.7 40,320 - EX31_RS15430 0.27 +1.1 40,320 - EX31_RS15430 0.27 +0.1 40,320 - EX31_RS15430 0.27 -1.4 40,341 + EX31_RS15430 0.29 +0.5 40,342 - EX31_RS15430 0.29 +1.0 40,376 + EX31_RS15430 0.32 +0.0 40,377 - EX31_RS15430 0.32 +1.4 40,380 - EX31_RS15430 0.32 -1.7 40,391 + EX31_RS15430 0.33 -0.8 40,391 + EX31_RS15430 0.33 -0.2 40,425 + EX31_RS15430 0.36 +0.6 40,425 + EX31_RS15430 0.36 +0.0 40,425 + EX31_RS15430 0.36 +0.5 40,425 + EX31_RS15430 0.36 +0.3 40,426 - EX31_RS15430 0.36 +0.5 40,426 - EX31_RS15430 0.36 +0.1 40,427 + EX31_RS15430 0.36 +0.8 40,427 + EX31_RS15430 0.36 -1.6 40,428 - EX31_RS15430 0.37 -0.6 40,428 - EX31_RS15430 0.37 -0.1 40,428 - EX31_RS15430 0.37 +0.1 40,487 + EX31_RS15430 0.42 +0.8 40,488 - EX31_RS15430 0.42 -0.1 40,488 - EX31_RS15430 0.42 +2.5 40,553 + EX31_RS15430 0.48 +0.6 40,554 - EX31_RS15430 0.48 +0.2 40,554 - EX31_RS15430 0.48 +0.3 40,554 - EX31_RS15430 0.48 +0.1 40,559 + EX31_RS15430 0.48 -0.2 40,559 + EX31_RS15430 0.48 -0.9 40,560 - EX31_RS15430 0.49 +0.7 40,590 + EX31_RS15430 0.51 +1.3 40,590 + EX31_RS15430 0.51 -0.7 40,590 + EX31_RS15430 0.51 +0.8 40,590 + EX31_RS15430 0.51 -0.1 40,591 - EX31_RS15430 0.51 +0.4 40,591 - EX31_RS15430 0.51 -0.4 40,591 - EX31_RS15430 0.51 +1.5 40,591 - EX31_RS15430 0.51 +0.1 40,657 + EX31_RS15430 0.57 +0.6 40,657 + EX31_RS15430 0.57 -0.7 40,657 + EX31_RS15430 0.57 -1.5 40,658 - EX31_RS15430 0.57 +1.1 40,658 - EX31_RS15430 0.57 -0.6 40,658 - EX31_RS15430 0.57 +1.5 40,658 - EX31_RS15430 0.57 +1.1
Or see this region's nucleotide sequence