Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22620 and EX31_RS22625 overlap by 4 nucleotides EX31_RS22625 and EX31_RS22630 are separated by 180 nucleotides EX31_RS22630 and EX31_RS22635 are separated by 111 nucleotides
EX31_RS22620: EX31_RS22620 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 280,199 to 281,263
_RS22620
EX31_RS22625: EX31_RS22625 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 281,260 to 281,769
_RS22625
EX31_RS22630: EX31_RS22630 - tRNA-(ms[2]io[6]A)-hydroxylase, at 281,950 to 282,582
_RS22630
EX31_RS22635: EX31_RS22635 - UDP-2,3-diacylglucosamine diphosphatase, at 282,694 to 283,416
_RS22635
Position (kb) 281
282 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 280.497 kb on + strand, within EX31_RS22620 at 280.497 kb on + strand, within EX31_RS22620 at 280.497 kb on + strand, within EX31_RS22620 at 280.497 kb on + strand, within EX31_RS22620 at 280.497 kb on + strand, within EX31_RS22620 at 280.498 kb on - strand, within EX31_RS22620 at 280.498 kb on - strand, within EX31_RS22620 at 280.498 kb on - strand, within EX31_RS22620 at 280.498 kb on - strand, within EX31_RS22620 at 280.608 kb on + strand, within EX31_RS22620 at 280.608 kb on + strand, within EX31_RS22620 at 280.609 kb on - strand, within EX31_RS22620 at 280.609 kb on - strand, within EX31_RS22620 at 280.609 kb on - strand, within EX31_RS22620 at 280.609 kb on - strand, within EX31_RS22620 at 280.609 kb on - strand, within EX31_RS22620 at 280.735 kb on - strand, within EX31_RS22620 at 280.747 kb on + strand, within EX31_RS22620 at 280.748 kb on - strand, within EX31_RS22620 at 280.824 kb on + strand, within EX31_RS22620 at 280.825 kb on - strand, within EX31_RS22620 at 280.843 kb on + strand, within EX31_RS22620 at 280.843 kb on + strand, within EX31_RS22620 at 280.844 kb on - strand, within EX31_RS22620 at 280.938 kb on - strand, within EX31_RS22620 at 281.182 kb on - strand at 281.404 kb on - strand, within EX31_RS22625 at 281.404 kb on - strand, within EX31_RS22625 at 281.456 kb on + strand, within EX31_RS22625 at 281.614 kb on - strand, within EX31_RS22625 at 281.667 kb on + strand, within EX31_RS22625 at 281.668 kb on - strand, within EX31_RS22625 at 281.668 kb on - strand, within EX31_RS22625 at 281.775 kb on + strand at 281.775 kb on + strand at 281.775 kb on + strand at 281.775 kb on + strand at 281.776 kb on - strand at 281.798 kb on - strand at 281.821 kb on + strand at 281.916 kb on + strand at 281.916 kb on + strand at 281.916 kb on + strand at 281.916 kb on + strand at 281.917 kb on - strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.949 kb on + strand at 281.963 kb on + strand at 282.060 kb on + strand, within EX31_RS22630 at 282.109 kb on + strand, within EX31_RS22630 at 282.110 kb on - strand, within EX31_RS22630 at 282.110 kb on - strand, within EX31_RS22630 at 282.110 kb on - strand, within EX31_RS22630 at 282.207 kb on + strand, within EX31_RS22630 at 282.207 kb on + strand, within EX31_RS22630 at 282.207 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.211 kb on - strand, within EX31_RS22630 at 282.211 kb on - strand, within EX31_RS22630 at 282.211 kb on - strand, within EX31_RS22630 at 282.211 kb on - strand, within EX31_RS22630 at 282.214 kb on + strand, within EX31_RS22630 at 282.214 kb on + strand, within EX31_RS22630 at 282.214 kb on + strand, within EX31_RS22630 at 282.214 kb on + strand, within EX31_RS22630 at 282.214 kb on + strand, within EX31_RS22630 at 282.215 kb on - strand, within EX31_RS22630 at 282.215 kb on - strand, within EX31_RS22630 at 282.215 kb on - strand, within EX31_RS22630 at 282.215 kb on - strand, within EX31_RS22630 at 282.216 kb on + strand, within EX31_RS22630 at 282.261 kb on + strand, within EX31_RS22630 at 282.261 kb on + strand, within EX31_RS22630 at 282.285 kb on + strand, within EX31_RS22630 at 282.285 kb on + strand, within EX31_RS22630 at 282.286 kb on - strand, within EX31_RS22630 at 282.286 kb on - strand, within EX31_RS22630 at 282.286 kb on - strand, within EX31_RS22630 at 282.316 kb on + strand, within EX31_RS22630 at 282.316 kb on + strand, within EX31_RS22630 at 282.317 kb on - strand, within EX31_RS22630 at 282.430 kb on + strand, within EX31_RS22630 at 282.451 kb on + strand, within EX31_RS22630 at 282.452 kb on - strand, within EX31_RS22630 at 282.567 kb on + strand at 282.567 kb on + strand at 282.567 kb on + strand at 282.568 kb on - strand at 282.568 kb on - strand at 282.568 kb on - strand at 282.568 kb on - strand at 282.568 kb on - strand at 282.568 kb on - strand at 282.615 kb on + strand at 282.616 kb on - strand at 282.616 kb on - strand at 282.623 kb on + strand at 282.623 kb on + strand at 282.624 kb on - strand at 282.624 kb on - strand at 282.671 kb on - strand at 282.680 kb on - strand at 282.680 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 280,497 + EX31_RS22620 0.28 -0.6 280,497 + EX31_RS22620 0.28 -2.1 280,497 + EX31_RS22620 0.28 -1.6 280,497 + EX31_RS22620 0.28 -0.5 280,497 + EX31_RS22620 0.28 -1.6 280,498 - EX31_RS22620 0.28 -1.3 280,498 - EX31_RS22620 0.28 -4.1 280,498 - EX31_RS22620 0.28 -0.5 280,498 - EX31_RS22620 0.28 -0.7 280,608 + EX31_RS22620 0.38 -0.3 280,608 + EX31_RS22620 0.38 -3.4 280,609 - EX31_RS22620 0.38 +0.3 280,609 - EX31_RS22620 0.38 -1.8 280,609 - EX31_RS22620 0.38 -1.7 280,609 - EX31_RS22620 0.38 -3.1 280,609 - EX31_RS22620 0.38 -2.0 280,735 - EX31_RS22620 0.50 -1.5 280,747 + EX31_RS22620 0.51 -0.9 280,748 - EX31_RS22620 0.52 -2.6 280,824 + EX31_RS22620 0.59 -1.5 280,825 - EX31_RS22620 0.59 +0.9 280,843 + EX31_RS22620 0.60 -2.3 280,843 + EX31_RS22620 0.60 -2.2 280,844 - EX31_RS22620 0.61 -1.3 280,938 - EX31_RS22620 0.69 -2.1 281,182 - -3.1 281,404 - EX31_RS22625 0.28 -2.1 281,404 - EX31_RS22625 0.28 -2.8 281,456 + EX31_RS22625 0.38 -2.8 281,614 - EX31_RS22625 0.69 -2.2 281,667 + EX31_RS22625 0.80 -1.1 281,668 - EX31_RS22625 0.80 -1.7 281,668 - EX31_RS22625 0.80 -2.7 281,775 + -1.7 281,775 + -2.7 281,775 + -3.5 281,775 + -1.3 281,776 - -2.9 281,798 - -2.2 281,821 + -4.2 281,916 + -0.6 281,916 + -2.2 281,916 + +0.9 281,916 + +0.3 281,917 - -0.7 281,918 + -2.3 281,918 + -0.9 281,918 + +1.0 281,918 + +0.4 281,918 + -1.7 281,918 + -0.1 281,918 + +1.7 281,918 + -0.1 281,918 + +0.3 281,918 + +1.2 281,918 + -0.1 281,918 + +0.1 281,919 - +0.3 281,919 - -0.2 281,919 - -0.4 281,919 - -1.5 281,919 - -0.3 281,919 - +0.5 281,919 - +0.3 281,919 - -0.2 281,919 - -0.8 281,949 + -0.6 281,963 + -0.3 282,060 + EX31_RS22630 0.17 +0.2 282,109 + EX31_RS22630 0.25 +0.3 282,110 - EX31_RS22630 0.25 +0.3 282,110 - EX31_RS22630 0.25 +1.3 282,110 - EX31_RS22630 0.25 +0.0 282,207 + EX31_RS22630 0.41 +0.6 282,207 + EX31_RS22630 0.41 -1.5 282,207 + EX31_RS22630 0.41 +1.7 282,210 + EX31_RS22630 0.41 +1.7 282,210 + EX31_RS22630 0.41 +0.2 282,210 + EX31_RS22630 0.41 +0.3 282,210 + EX31_RS22630 0.41 +1.0 282,210 + EX31_RS22630 0.41 +0.4 282,210 + EX31_RS22630 0.41 -1.1 282,210 + EX31_RS22630 0.41 -0.3 282,210 + EX31_RS22630 0.41 +1.1 282,210 + EX31_RS22630 0.41 -0.3 282,210 + EX31_RS22630 0.41 +0.3 282,210 + EX31_RS22630 0.41 +0.4 282,210 + EX31_RS22630 0.41 -0.6 282,211 - EX31_RS22630 0.41 -0.0 282,211 - EX31_RS22630 0.41 -0.4 282,211 - EX31_RS22630 0.41 -0.2 282,211 - EX31_RS22630 0.41 +0.6 282,214 + EX31_RS22630 0.42 +0.4 282,214 + EX31_RS22630 0.42 +0.1 282,214 + EX31_RS22630 0.42 -0.0 282,214 + EX31_RS22630 0.42 +1.2 282,214 + EX31_RS22630 0.42 +0.9 282,215 - EX31_RS22630 0.42 +0.0 282,215 - EX31_RS22630 0.42 +1.9 282,215 - EX31_RS22630 0.42 +0.8 282,215 - EX31_RS22630 0.42 +2.5 282,216 + EX31_RS22630 0.42 +0.7 282,261 + EX31_RS22630 0.49 -0.7 282,261 + EX31_RS22630 0.49 -1.0 282,285 + EX31_RS22630 0.53 -0.3 282,285 + EX31_RS22630 0.53 +0.7 282,286 - EX31_RS22630 0.53 -1.0 282,286 - EX31_RS22630 0.53 +1.9 282,286 - EX31_RS22630 0.53 +1.5 282,316 + EX31_RS22630 0.58 -0.5 282,316 + EX31_RS22630 0.58 -0.4 282,317 - EX31_RS22630 0.58 -0.0 282,430 + EX31_RS22630 0.76 -0.1 282,451 + EX31_RS22630 0.79 +0.1 282,452 - EX31_RS22630 0.79 -0.3 282,567 + -1.5 282,567 + +1.0 282,567 + +0.7 282,568 - +0.8 282,568 - +0.5 282,568 - +1.1 282,568 - -2.0 282,568 - +0.1 282,568 - +2.4 282,615 + +0.9 282,616 - +1.0 282,616 - +0.9 282,623 + -1.4 282,623 + -0.7 282,624 - -0.5 282,624 - -2.1 282,671 - -0.1 282,680 - -0.6 282,680 - +1.0
Or see this region's nucleotide sequence