Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18615 and EX31_RS18620 are separated by 22 nucleotides EX31_RS18620 and EX31_RS18625 overlap by 4 nucleotides EX31_RS18625 and EX31_RS18630 are separated by 187 nucleotides EX31_RS18630 and EX31_RS18635 are separated by 210 nucleotides
EX31_RS18615: EX31_RS18615 - NAD(P)-dependent oxidoreductase, at 302,840 to 303,874
_RS18615
EX31_RS18620: EX31_RS18620 - N-acetylmuramoyl-L-alanine amidase, at 303,897 to 304,745
_RS18620
EX31_RS18625: EX31_RS18625 - heavy metal-binding domain-containing protein, at 304,742 to 305,068
_RS18625
EX31_RS18630: EX31_RS18630 - lipoprotein, at 305,256 to 305,837
_RS18630
EX31_RS18635: EX31_RS18635 - arginine ABC transporter ATP-binding protein ArtP, at 306,048 to 306,776
_RS18635
Position (kb) 304
305
306 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 303.767 kb on + strand, within EX31_RS18615 at 303.832 kb on + strand at 303.833 kb on - strand at 303.878 kb on + strand at 303.878 kb on + strand at 303.878 kb on + strand at 303.879 kb on - strand at 303.924 kb on + strand at 304.037 kb on + strand, within EX31_RS18620 at 304.037 kb on + strand, within EX31_RS18620 at 304.037 kb on + strand, within EX31_RS18620 at 304.038 kb on - strand, within EX31_RS18620 at 304.038 kb on - strand, within EX31_RS18620 at 304.040 kb on + strand, within EX31_RS18620 at 304.061 kb on - strand, within EX31_RS18620 at 304.113 kb on - strand, within EX31_RS18620 at 304.261 kb on - strand, within EX31_RS18620 at 304.302 kb on + strand, within EX31_RS18620 at 304.303 kb on - strand, within EX31_RS18620 at 304.303 kb on - strand, within EX31_RS18620 at 304.308 kb on + strand, within EX31_RS18620 at 304.308 kb on + strand, within EX31_RS18620 at 304.331 kb on - strand, within EX31_RS18620 at 304.351 kb on + strand, within EX31_RS18620 at 304.351 kb on + strand, within EX31_RS18620 at 304.352 kb on - strand, within EX31_RS18620 at 304.352 kb on - strand, within EX31_RS18620 at 304.352 kb on - strand, within EX31_RS18620 at 304.352 kb on - strand, within EX31_RS18620 at 304.352 kb on - strand, within EX31_RS18620 at 304.352 kb on - strand, within EX31_RS18620 at 304.360 kb on + strand, within EX31_RS18620 at 304.361 kb on - strand, within EX31_RS18620 at 304.394 kb on + strand, within EX31_RS18620 at 304.394 kb on + strand, within EX31_RS18620 at 304.395 kb on - strand, within EX31_RS18620 at 304.452 kb on - strand, within EX31_RS18620 at 304.519 kb on + strand, within EX31_RS18620 at 304.519 kb on + strand, within EX31_RS18620 at 304.520 kb on - strand, within EX31_RS18620 at 304.520 kb on - strand, within EX31_RS18620 at 304.520 kb on - strand, within EX31_RS18620 at 304.520 kb on - strand, within EX31_RS18620 at 304.520 kb on - strand, within EX31_RS18620 at 304.520 kb on - strand, within EX31_RS18620 at 304.551 kb on - strand, within EX31_RS18620 at 304.551 kb on - strand, within EX31_RS18620 at 304.551 kb on - strand, within EX31_RS18620 at 304.551 kb on - strand, within EX31_RS18620 at 304.554 kb on + strand, within EX31_RS18620 at 304.554 kb on + strand, within EX31_RS18620 at 304.582 kb on + strand, within EX31_RS18620 at 304.582 kb on + strand, within EX31_RS18620 at 304.582 kb on + strand, within EX31_RS18620 at 304.583 kb on - strand, within EX31_RS18620 at 304.702 kb on - strand at 304.826 kb on - strand, within EX31_RS18625 at 304.920 kb on + strand, within EX31_RS18625 at 305.009 kb on + strand, within EX31_RS18625 at 305.019 kb on + strand, within EX31_RS18625 at 305.020 kb on - strand, within EX31_RS18625 at 305.072 kb on + strand at 305.073 kb on - strand at 305.073 kb on - strand at 305.073 kb on - strand at 305.073 kb on - strand at 305.073 kb on - strand at 305.106 kb on + strand at 305.106 kb on + strand at 305.106 kb on + strand at 305.107 kb on - strand at 305.107 kb on - strand at 305.107 kb on - strand at 305.115 kb on + strand at 305.115 kb on + strand at 305.116 kb on - strand at 305.116 kb on - strand at 305.116 kb on - strand at 305.118 kb on + strand at 305.118 kb on + strand at 305.118 kb on + strand at 305.118 kb on + strand at 305.118 kb on + strand at 305.119 kb on - strand at 305.119 kb on - strand at 305.119 kb on - strand at 305.119 kb on - strand at 305.119 kb on - strand at 305.119 kb on - strand at 305.119 kb on - strand at 305.124 kb on - strand at 305.124 kb on - strand at 305.124 kb on - strand at 305.147 kb on + strand at 305.147 kb on + strand at 305.147 kb on + strand at 305.148 kb on - strand at 305.148 kb on - strand at 305.148 kb on - strand at 305.150 kb on + strand at 305.151 kb on - strand at 305.151 kb on - strand at 305.182 kb on - strand at 305.197 kb on + strand at 305.197 kb on + strand at 305.197 kb on + strand at 305.197 kb on + strand at 305.198 kb on - strand at 305.198 kb on - strand at 305.198 kb on - strand at 305.198 kb on - strand at 305.198 kb on - strand at 305.198 kb on - strand at 305.199 kb on + strand at 305.200 kb on - strand at 305.200 kb on - strand at 305.204 kb on + strand at 305.204 kb on + strand at 305.204 kb on + strand at 305.204 kb on + strand at 305.205 kb on - strand at 305.205 kb on - strand at 305.205 kb on - strand at 305.205 kb on - strand at 305.205 kb on - strand at 305.205 kb on - strand at 305.205 kb on - strand at 305.209 kb on + strand at 305.252 kb on + strand at 305.252 kb on + strand at 305.252 kb on + strand at 305.252 kb on + strand at 305.252 kb on + strand at 305.252 kb on + strand at 305.252 kb on + strand at 305.253 kb on - strand at 305.253 kb on - strand at 305.293 kb on + strand at 305.302 kb on + strand at 305.302 kb on + strand at 305.303 kb on - strand at 305.303 kb on - strand at 305.303 kb on - strand at 305.303 kb on - strand at 305.303 kb on - strand at 305.303 kb on - strand at 305.320 kb on + strand, within EX31_RS18630 at 305.321 kb on - strand, within EX31_RS18630 at 305.331 kb on + strand, within EX31_RS18630 at 305.331 kb on + strand, within EX31_RS18630 at 305.331 kb on + strand, within EX31_RS18630 at 305.331 kb on + strand, within EX31_RS18630 at 305.331 kb on + strand, within EX31_RS18630 at 305.331 kb on + strand, within EX31_RS18630 at 305.331 kb on + strand, within EX31_RS18630 at 305.331 kb on + strand, within EX31_RS18630 at 305.332 kb on - strand, within EX31_RS18630 at 305.332 kb on - strand, within EX31_RS18630 at 305.332 kb on - strand, within EX31_RS18630 at 305.332 kb on - strand, within EX31_RS18630 at 305.332 kb on - strand, within EX31_RS18630 at 305.332 kb on - strand, within EX31_RS18630 at 305.332 kb on - strand, within EX31_RS18630 at 305.332 kb on - strand, within EX31_RS18630 at 305.332 kb on - strand, within EX31_RS18630 at 305.333 kb on + strand, within EX31_RS18630 at 305.333 kb on + strand, within EX31_RS18630 at 305.333 kb on + strand, within EX31_RS18630 at 305.333 kb on + strand, within EX31_RS18630 at 305.333 kb on + strand, within EX31_RS18630 at 305.333 kb on + strand, within EX31_RS18630 at 305.333 kb on + strand, within EX31_RS18630 at 305.333 kb on + strand, within EX31_RS18630 at 305.334 kb on - strand, within EX31_RS18630 at 305.334 kb on - strand, within EX31_RS18630 at 305.334 kb on - strand, within EX31_RS18630 at 305.334 kb on - strand, within EX31_RS18630 at 305.334 kb on - strand, within EX31_RS18630 at 305.334 kb on - strand, within EX31_RS18630 at 305.334 kb on - strand, within EX31_RS18630 at 305.334 kb on - strand, within EX31_RS18630 at 305.334 kb on - strand, within EX31_RS18630 at 305.334 kb on - strand, within EX31_RS18630 at 305.334 kb on - strand, within EX31_RS18630 at 305.400 kb on + strand, within EX31_RS18630 at 305.415 kb on - strand, within EX31_RS18630 at 305.415 kb on - strand, within EX31_RS18630 at 305.434 kb on + strand, within EX31_RS18630 at 305.469 kb on + strand, within EX31_RS18630 at 305.469 kb on + strand, within EX31_RS18630 at 305.470 kb on - strand, within EX31_RS18630 at 305.470 kb on - strand, within EX31_RS18630 at 305.487 kb on + strand, within EX31_RS18630 at 305.488 kb on - strand, within EX31_RS18630 at 305.488 kb on - strand, within EX31_RS18630 at 305.488 kb on - strand, within EX31_RS18630 at 305.488 kb on - strand, within EX31_RS18630 at 305.488 kb on - strand, within EX31_RS18630 at 305.620 kb on + strand, within EX31_RS18630 at 305.638 kb on + strand, within EX31_RS18630 at 305.775 kb on + strand, within EX31_RS18630 at 305.775 kb on + strand, within EX31_RS18630 at 305.775 kb on + strand, within EX31_RS18630 at 305.775 kb on + strand, within EX31_RS18630 at 305.775 kb on + strand, within EX31_RS18630 at 305.775 kb on + strand, within EX31_RS18630 at 305.777 kb on + strand, within EX31_RS18630 at 305.777 kb on + strand, within EX31_RS18630 at 305.777 kb on + strand, within EX31_RS18630 at 305.777 kb on + strand, within EX31_RS18630 at 305.778 kb on - strand, within EX31_RS18630 at 305.835 kb on + strand at 305.852 kb on + strand at 305.857 kb on + strand at 305.868 kb on + strand at 305.868 kb on + strand at 305.868 kb on + strand at 305.869 kb on - strand at 305.869 kb on - strand at 305.869 kb on - strand at 305.869 kb on - strand at 305.869 kb on - strand at 305.888 kb on + strand at 305.924 kb on - strand at 305.924 kb on - strand at 305.929 kb on + strand at 305.929 kb on + strand at 305.929 kb on + strand at 305.929 kb on + strand at 305.929 kb on + strand at 305.929 kb on + strand at 305.929 kb on + strand at 305.930 kb on - strand at 305.930 kb on - strand at 305.930 kb on - strand at 305.940 kb on - strand at 305.940 kb on - strand at 305.940 kb on - strand at 305.940 kb on - strand at 305.967 kb on + strand at 305.967 kb on + strand at 305.968 kb on - strand at 305.968 kb on - strand at 305.992 kb on + strand at 305.992 kb on + strand at 305.992 kb on + strand at 305.992 kb on + strand at 305.992 kb on + strand at 305.993 kb on - strand at 305.993 kb on - strand at 305.993 kb on - strand at 305.993 kb on - strand at 305.993 kb on - strand at 305.996 kb on + strand at 305.996 kb on + strand at 305.996 kb on + strand at 305.996 kb on + strand at 305.996 kb on + strand at 305.996 kb on + strand at 305.996 kb on + strand at 305.997 kb on - strand at 305.997 kb on - strand at 305.997 kb on - strand at 305.997 kb on - strand at 305.997 kb on - strand at 306.010 kb on + strand at 306.010 kb on + strand at 306.011 kb on - strand at 306.011 kb on - strand at 306.033 kb on - strand at 306.039 kb on + strand at 306.053 kb on + strand at 306.062 kb on + strand at 306.063 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 303,767 + EX31_RS18615 0.90 -0.1 303,832 + -0.1 303,833 - +0.7 303,878 + +0.9 303,878 + +1.1 303,878 + +0.3 303,879 - +1.1 303,924 + +0.9 304,037 + EX31_RS18620 0.16 +0.1 304,037 + EX31_RS18620 0.16 +1.4 304,037 + EX31_RS18620 0.16 +0.5 304,038 - EX31_RS18620 0.17 -0.3 304,038 - EX31_RS18620 0.17 -3.1 304,040 + EX31_RS18620 0.17 +0.6 304,061 - EX31_RS18620 0.19 -1.8 304,113 - EX31_RS18620 0.25 +1.4 304,261 - EX31_RS18620 0.43 -1.0 304,302 + EX31_RS18620 0.48 -0.5 304,303 - EX31_RS18620 0.48 -0.8 304,303 - EX31_RS18620 0.48 +0.8 304,308 + EX31_RS18620 0.48 +4.2 304,308 + EX31_RS18620 0.48 -0.7 304,331 - EX31_RS18620 0.51 -0.7 304,351 + EX31_RS18620 0.53 +1.5 304,351 + EX31_RS18620 0.53 +0.9 304,352 - EX31_RS18620 0.54 -0.5 304,352 - EX31_RS18620 0.54 -1.3 304,352 - EX31_RS18620 0.54 +0.6 304,352 - EX31_RS18620 0.54 +0.6 304,352 - EX31_RS18620 0.54 -0.7 304,352 - EX31_RS18620 0.54 -0.2 304,360 + EX31_RS18620 0.55 -0.8 304,361 - EX31_RS18620 0.55 +0.2 304,394 + EX31_RS18620 0.59 +0.3 304,394 + EX31_RS18620 0.59 +0.7 304,395 - EX31_RS18620 0.59 -0.1 304,452 - EX31_RS18620 0.65 -0.5 304,519 + EX31_RS18620 0.73 +0.7 304,519 + EX31_RS18620 0.73 -0.4 304,520 - EX31_RS18620 0.73 -0.7 304,520 - EX31_RS18620 0.73 -0.7 304,520 - EX31_RS18620 0.73 -0.1 304,520 - EX31_RS18620 0.73 +0.1 304,520 - EX31_RS18620 0.73 -0.6 304,520 - EX31_RS18620 0.73 +0.2 304,551 - EX31_RS18620 0.77 +0.3 304,551 - EX31_RS18620 0.77 +0.0 304,551 - EX31_RS18620 0.77 -0.3 304,551 - EX31_RS18620 0.77 -1.0 304,554 + EX31_RS18620 0.77 +1.7 304,554 + EX31_RS18620 0.77 +0.4 304,582 + EX31_RS18620 0.81 +0.6 304,582 + EX31_RS18620 0.81 -0.1 304,582 + EX31_RS18620 0.81 +0.4 304,583 - EX31_RS18620 0.81 -0.1 304,702 - -0.2 304,826 - EX31_RS18625 0.26 +1.9 304,920 + EX31_RS18625 0.54 -0.3 305,009 + EX31_RS18625 0.82 +0.1 305,019 + EX31_RS18625 0.85 -0.5 305,020 - EX31_RS18625 0.85 +0.1 305,072 + +0.1 305,073 - -2.1 305,073 - -0.7 305,073 - +0.3 305,073 - +1.1 305,073 - -0.1 305,106 + -0.0 305,106 + +0.9 305,106 + +0.0 305,107 - -0.6 305,107 - -0.5 305,107 - +0.3 305,115 + +1.6 305,115 + -0.4 305,116 - -0.5 305,116 - -3.3 305,116 - -1.4 305,118 + -0.7 305,118 + -1.3 305,118 + +1.3 305,118 + +0.9 305,118 + +0.8 305,119 - -0.8 305,119 - -0.2 305,119 - +0.4 305,119 - -0.6 305,119 - -0.0 305,119 - -0.3 305,119 - -0.3 305,124 - +0.9 305,124 - -1.3 305,124 - -0.1 305,147 + +1.0 305,147 + -2.1 305,147 + +0.6 305,148 - -0.5 305,148 - -1.1 305,148 - +0.2 305,150 + +0.9 305,151 - +1.4 305,151 - +0.0 305,182 - -2.0 305,197 + +0.3 305,197 + +0.9 305,197 + +1.2 305,197 + -0.0 305,198 - +0.8 305,198 - +0.8 305,198 - +1.3 305,198 - -0.1 305,198 - +0.5 305,198 - +0.9 305,199 + -0.2 305,200 - +0.2 305,200 - -1.3 305,204 + -0.3 305,204 + +0.5 305,204 + +0.4 305,204 + -0.5 305,205 - -0.3 305,205 - +1.0 305,205 - -0.3 305,205 - -1.2 305,205 - -0.0 305,205 - +0.1 305,205 - +0.5 305,209 + -1.8 305,252 + +0.3 305,252 + -0.2 305,252 + +0.7 305,252 + +1.6 305,252 + +0.4 305,252 + +0.7 305,252 + +0.4 305,253 - -0.1 305,253 - +0.9 305,293 + +1.4 305,302 + -1.3 305,302 + -0.3 305,303 - -0.4 305,303 - +0.9 305,303 - +0.5 305,303 - +0.1 305,303 - +1.2 305,303 - +0.2 305,320 + EX31_RS18630 0.11 +0.9 305,321 - EX31_RS18630 0.11 -0.5 305,331 + EX31_RS18630 0.13 -0.7 305,331 + EX31_RS18630 0.13 +0.2 305,331 + EX31_RS18630 0.13 +2.1 305,331 + EX31_RS18630 0.13 -0.1 305,331 + EX31_RS18630 0.13 +0.3 305,331 + EX31_RS18630 0.13 +0.7 305,331 + EX31_RS18630 0.13 -0.9 305,331 + EX31_RS18630 0.13 +1.0 305,332 - EX31_RS18630 0.13 -0.4 305,332 - EX31_RS18630 0.13 -0.3 305,332 - EX31_RS18630 0.13 -0.5 305,332 - EX31_RS18630 0.13 +0.2 305,332 - EX31_RS18630 0.13 +0.8 305,332 - EX31_RS18630 0.13 -1.1 305,332 - EX31_RS18630 0.13 +0.9 305,332 - EX31_RS18630 0.13 +1.4 305,332 - EX31_RS18630 0.13 +0.3 305,333 + EX31_RS18630 0.13 +1.3 305,333 + EX31_RS18630 0.13 -0.6 305,333 + EX31_RS18630 0.13 -2.0 305,333 + EX31_RS18630 0.13 +0.8 305,333 + EX31_RS18630 0.13 +0.2 305,333 + EX31_RS18630 0.13 +0.3 305,333 + EX31_RS18630 0.13 +0.2 305,333 + EX31_RS18630 0.13 +1.3 305,334 - EX31_RS18630 0.13 +0.7 305,334 - EX31_RS18630 0.13 +3.2 305,334 - EX31_RS18630 0.13 +0.7 305,334 - EX31_RS18630 0.13 +0.2 305,334 - EX31_RS18630 0.13 -0.3 305,334 - EX31_RS18630 0.13 +0.3 305,334 - EX31_RS18630 0.13 -0.7 305,334 - EX31_RS18630 0.13 +0.0 305,334 - EX31_RS18630 0.13 -0.3 305,334 - EX31_RS18630 0.13 -1.2 305,334 - EX31_RS18630 0.13 +1.3 305,400 + EX31_RS18630 0.25 -1.0 305,415 - EX31_RS18630 0.27 -1.1 305,415 - EX31_RS18630 0.27 +0.4 305,434 + EX31_RS18630 0.31 +0.3 305,469 + EX31_RS18630 0.37 -1.9 305,469 + EX31_RS18630 0.37 +0.9 305,470 - EX31_RS18630 0.37 +1.0 305,470 - EX31_RS18630 0.37 -0.3 305,487 + EX31_RS18630 0.40 +2.2 305,488 - EX31_RS18630 0.40 -0.7 305,488 - EX31_RS18630 0.40 +0.5 305,488 - EX31_RS18630 0.40 +0.9 305,488 - EX31_RS18630 0.40 -0.5 305,488 - EX31_RS18630 0.40 -0.2 305,620 + EX31_RS18630 0.63 +1.7 305,638 + EX31_RS18630 0.66 +0.7 305,775 + EX31_RS18630 0.89 +1.3 305,775 + EX31_RS18630 0.89 -0.0 305,775 + EX31_RS18630 0.89 -0.4 305,775 + EX31_RS18630 0.89 +1.0 305,775 + EX31_RS18630 0.89 +0.4 305,775 + EX31_RS18630 0.89 -0.4 305,777 + EX31_RS18630 0.90 +0.6 305,777 + EX31_RS18630 0.90 -1.0 305,777 + EX31_RS18630 0.90 +2.6 305,777 + EX31_RS18630 0.90 +0.3 305,778 - EX31_RS18630 0.90 +1.3 305,835 + +1.0 305,852 + -0.0 305,857 + +0.2 305,868 + +0.9 305,868 + +0.1 305,868 + -0.6 305,869 - -0.0 305,869 - +0.4 305,869 - +0.9 305,869 - +1.3 305,869 - +0.2 305,888 + +2.1 305,924 - +0.3 305,924 - +0.0 305,929 + +0.0 305,929 + -0.0 305,929 + -0.7 305,929 + -0.0 305,929 + +0.4 305,929 + +0.3 305,929 + +0.4 305,930 - -0.1 305,930 - +0.4 305,930 - +0.8 305,940 - +0.1 305,940 - +1.0 305,940 - -0.0 305,940 - -0.3 305,967 + +0.1 305,967 + +0.7 305,968 - -0.6 305,968 - -0.1 305,992 + +0.2 305,992 + +0.5 305,992 + +0.3 305,992 + -1.8 305,992 + -0.7 305,993 - -0.5 305,993 - +0.3 305,993 - -0.1 305,993 - -2.1 305,993 - -0.0 305,996 + -0.5 305,996 + +0.4 305,996 + +0.3 305,996 + +0.5 305,996 + +1.3 305,996 + +0.3 305,996 + +0.8 305,997 - -0.0 305,997 - +0.0 305,997 - -0.8 305,997 - -0.1 305,997 - -0.2 306,010 + -1.3 306,010 + -1.7 306,011 - +0.2 306,011 - +0.3 306,033 - +1.3 306,039 + +0.5 306,053 + -0.7 306,062 + -0.2 306,063 - -0.5
Or see this region's nucleotide sequence