Strain Fitness in Rahnella sp. WP5 around EX31_RS14340

Experiment: L-Glutamine (N); ShewMM media; shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS14330 and EX31_RS14335 are separated by 52 nucleotidesEX31_RS14335 and EX31_RS14340 are separated by 87 nucleotidesEX31_RS14340 and EX31_RS14345 overlap by 1 nucleotidesEX31_RS14345 and EX31_RS14350 are separated by 64 nucleotidesEX31_RS14350 and EX31_RS14355 are separated by 53 nucleotides EX31_RS14330: EX31_RS14330 - tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL, at 47,992 to 48,465 _RS14330 EX31_RS14335: EX31_RS14335 - serine O-acetyltransferase, at 48,518 to 49,339 _RS14335 EX31_RS14340: EX31_RS14340 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 49,427 to 50,446 _RS14340 EX31_RS14345: EX31_RS14345 - protein-export chaperone SecB, at 50,446 to 50,916 _RS14345 EX31_RS14350: EX31_RS14350 - glutaredoxin 3, at 50,981 to 51,232 _RS14350 EX31_RS14355: EX31_RS14355 - rhodanese-like domain-containing protein, at 51,286 to 51,717 _RS14355 Position (kb) 49 50 51Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 48.464 kb on - strandat 48.553 kb on + strandat 48.553 kb on + strandat 48.554 kb on - strandat 48.574 kb on + strandat 48.575 kb on - strandat 48.707 kb on - strand, within EX31_RS14335at 48.707 kb on - strand, within EX31_RS14335at 48.757 kb on + strand, within EX31_RS14335at 48.788 kb on - strand, within EX31_RS14335at 48.819 kb on - strand, within EX31_RS14335at 48.910 kb on + strand, within EX31_RS14335at 48.911 kb on - strand, within EX31_RS14335at 48.914 kb on + strand, within EX31_RS14335at 48.995 kb on - strand, within EX31_RS14335at 49.048 kb on - strand, within EX31_RS14335at 49.200 kb on + strand, within EX31_RS14335at 49.200 kb on + strand, within EX31_RS14335at 49.201 kb on - strand, within EX31_RS14335at 49.300 kb on + strandat 49.300 kb on + strandat 49.301 kb on - strandat 49.306 kb on + strandat 49.322 kb on + strandat 49.323 kb on - strandat 49.396 kb on - strandat 49.550 kb on - strand, within EX31_RS14340at 49.727 kb on - strand, within EX31_RS14340at 50.187 kb on - strand, within EX31_RS14340at 50.216 kb on - strand, within EX31_RS14340at 50.316 kb on - strand, within EX31_RS14340at 50.616 kb on - strand, within EX31_RS14345at 50.662 kb on - strand, within EX31_RS14345at 50.752 kb on + strand, within EX31_RS14345at 50.752 kb on + strand, within EX31_RS14345at 50.753 kb on - strand, within EX31_RS14345at 50.753 kb on - strand, within EX31_RS14345at 50.868 kb on - strand, within EX31_RS14345at 50.871 kb on + strandat 50.872 kb on - strandat 50.872 kb on - strandat 50.872 kb on - strandat 50.923 kb on - strandat 50.923 kb on - strandat 50.923 kb on - strandat 50.926 kb on - strandat 50.926 kb on - strandat 50.940 kb on - strandat 50.940 kb on - strandat 50.940 kb on - strandat 50.940 kb on - strandat 50.949 kb on - strandat 50.957 kb on - strandat 50.957 kb on - strandat 50.980 kb on - strandat 50.980 kb on - strandat 51.027 kb on - strand, within EX31_RS14350at 51.027 kb on - strand, within EX31_RS14350at 51.027 kb on - strand, within EX31_RS14350at 51.027 kb on - strand, within EX31_RS14350at 51.027 kb on - strand, within EX31_RS14350at 51.027 kb on - strand, within EX31_RS14350at 51.027 kb on - strand, within EX31_RS14350at 51.027 kb on - strand, within EX31_RS14350at 51.116 kb on - strand, within EX31_RS14350at 51.116 kb on - strand, within EX31_RS14350at 51.116 kb on - strand, within EX31_RS14350at 51.164 kb on - strand, within EX31_RS14350at 51.164 kb on - strand, within EX31_RS14350at 51.167 kb on - strand, within EX31_RS14350at 51.167 kb on - strand, within EX31_RS14350at 51.167 kb on - strand, within EX31_RS14350at 51.167 kb on - strand, within EX31_RS14350at 51.214 kb on - strandat 51.214 kb on - strandat 51.214 kb on - strandat 51.267 kb on + strandat 51.279 kb on + strandat 51.280 kb on - strandat 51.280 kb on - strandat 51.280 kb on - strandat 51.280 kb on - strandat 51.280 kb on - strandat 51.288 kb on - strandat 51.288 kb on - strandat 51.288 kb on - strandat 51.288 kb on - strandat 51.301 kb on + strandat 51.301 kb on + strandat 51.301 kb on + strandat 51.301 kb on + strandat 51.302 kb on - strandat 51.333 kb on + strand, within EX31_RS14355at 51.334 kb on - strand, within EX31_RS14355at 51.334 kb on - strand, within EX31_RS14355at 51.334 kb on - strand, within EX31_RS14355at 51.348 kb on + strand, within EX31_RS14355at 51.349 kb on - strand, within EX31_RS14355at 51.349 kb on - strand, within EX31_RS14355at 51.427 kb on - strand, within EX31_RS14355at 51.442 kb on - strand, within EX31_RS14355at 51.442 kb on - strand, within EX31_RS14355at 51.442 kb on - strand, within EX31_RS14355at 51.442 kb on - strand, within EX31_RS14355at 51.442 kb on - strand, within EX31_RS14355

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking
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48,464 - -0.6
48,553 + -3.6
48,553 + -2.9
48,554 - +0.3
48,574 + -1.8
48,575 - -0.1
48,707 - EX31_RS14335 0.23 +0.9
48,707 - EX31_RS14335 0.23 -0.7
48,757 + EX31_RS14335 0.29 +1.5
48,788 - EX31_RS14335 0.33 +0.5
48,819 - EX31_RS14335 0.37 -0.3
48,910 + EX31_RS14335 0.48 +0.2
48,911 - EX31_RS14335 0.48 +0.2
48,914 + EX31_RS14335 0.48 -0.8
48,995 - EX31_RS14335 0.58 -1.8
49,048 - EX31_RS14335 0.64 -2.1
49,200 + EX31_RS14335 0.83 -0.5
49,200 + EX31_RS14335 0.83 +0.5
49,201 - EX31_RS14335 0.83 -1.6
49,300 + +0.9
49,300 + -3.0
49,301 - -0.1
49,306 + -2.9
49,322 + -1.8
49,323 - -0.7
49,396 - +0.0
49,550 - EX31_RS14340 0.12 +0.5
49,727 - EX31_RS14340 0.29 -0.5
50,187 - EX31_RS14340 0.75 -0.1
50,216 - EX31_RS14340 0.77 -1.0
50,316 - EX31_RS14340 0.87 -1.2
50,616 - EX31_RS14345 0.36 -1.2
50,662 - EX31_RS14345 0.46 -0.0
50,752 + EX31_RS14345 0.65 +0.9
50,752 + EX31_RS14345 0.65 -0.1
50,753 - EX31_RS14345 0.65 +1.3
50,753 - EX31_RS14345 0.65 -0.8
50,868 - EX31_RS14345 0.90 -0.4
50,871 + +0.5
50,872 - -0.2
50,872 - +2.7
50,872 - -0.3
50,923 - +1.1
50,923 - +0.5
50,923 - +0.7
50,926 - +0.5
50,926 - +1.6
50,940 - -0.0
50,940 - +1.4
50,940 - +0.3
50,940 - +0.8
50,949 - +0.3
50,957 - +0.3
50,957 - +0.2
50,980 - +0.8
50,980 - +1.0
51,027 - EX31_RS14350 0.18 -0.3
51,027 - EX31_RS14350 0.18 +0.4
51,027 - EX31_RS14350 0.18 -0.1
51,027 - EX31_RS14350 0.18 -1.1
51,027 - EX31_RS14350 0.18 +0.5
51,027 - EX31_RS14350 0.18 -0.8
51,027 - EX31_RS14350 0.18 -0.6
51,027 - EX31_RS14350 0.18 +0.8
51,116 - EX31_RS14350 0.54 -1.4
51,116 - EX31_RS14350 0.54 +0.9
51,116 - EX31_RS14350 0.54 +1.2
51,164 - EX31_RS14350 0.73 -0.3
51,164 - EX31_RS14350 0.73 -1.2
51,167 - EX31_RS14350 0.74 +0.0
51,167 - EX31_RS14350 0.74 +0.4
51,167 - EX31_RS14350 0.74 +1.4
51,167 - EX31_RS14350 0.74 +0.3
51,214 - +0.0
51,214 - +0.6
51,214 - -0.6
51,267 + -2.4
51,279 + -1.6
51,280 - -0.1
51,280 - +2.5
51,280 - +1.2
51,280 - +0.8
51,280 - -0.4
51,288 - +0.5
51,288 - +0.4
51,288 - -0.1
51,288 - -0.1
51,301 + -1.3
51,301 + -2.7
51,301 + -2.0
51,301 + +2.2
51,302 - +0.5
51,333 + EX31_RS14355 0.11 -1.1
51,334 - EX31_RS14355 0.11 +0.4
51,334 - EX31_RS14355 0.11 +1.1
51,334 - EX31_RS14355 0.11 -0.7
51,348 + EX31_RS14355 0.14 +0.5
51,349 - EX31_RS14355 0.15 +0.8
51,349 - EX31_RS14355 0.15 +1.3
51,427 - EX31_RS14355 0.33 +0.7
51,442 - EX31_RS14355 0.36 -0.2
51,442 - EX31_RS14355 0.36 -0.0
51,442 - EX31_RS14355 0.36 +0.9
51,442 - EX31_RS14355 0.36 -0.2
51,442 - EX31_RS14355 0.36 +1.4

Or see this region's nucleotide sequence