Strain Fitness in Rahnella sp. WP5 around EX31_RS12005

Experiment: L-Glutamine (N); ShewMM media; shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS12000 and EX31_RS12005 are separated by 100 nucleotidesEX31_RS12005 and EX31_RS12010 are separated by 206 nucleotides EX31_RS12000: EX31_RS12000 - amidohydrolase family protein, at 63,382 to 64,800 _RS12000 EX31_RS12005: EX31_RS12005 - LysR family transcriptional regulator, at 64,901 to 65,755 _RS12005 EX31_RS12010: EX31_RS12010 - cysteine protease, at 65,962 to 66,651 _RS12010 Position (kb) 64 65 66Strain fitness (log2 ratio) -2 -1 0 1 2at 63.902 kb on + strand, within EX31_RS12000at 63.902 kb on + strand, within EX31_RS12000at 63.902 kb on + strand, within EX31_RS12000at 63.902 kb on + strand, within EX31_RS12000at 63.902 kb on + strand, within EX31_RS12000at 63.902 kb on + strand, within EX31_RS12000at 63.902 kb on + strand, within EX31_RS12000at 63.903 kb on - strand, within EX31_RS12000at 63.903 kb on - strand, within EX31_RS12000at 63.903 kb on - strand, within EX31_RS12000at 63.903 kb on - strand, within EX31_RS12000at 63.903 kb on - strand, within EX31_RS12000at 63.903 kb on - strand, within EX31_RS12000at 63.949 kb on - strand, within EX31_RS12000at 63.949 kb on - strand, within EX31_RS12000at 63.949 kb on - strand, within EX31_RS12000at 63.949 kb on - strand, within EX31_RS12000at 63.961 kb on - strand, within EX31_RS12000at 64.046 kb on + strand, within EX31_RS12000at 64.046 kb on + strand, within EX31_RS12000at 64.046 kb on + strand, within EX31_RS12000at 64.046 kb on + strand, within EX31_RS12000at 64.046 kb on + strand, within EX31_RS12000at 64.046 kb on + strand, within EX31_RS12000at 64.047 kb on - strand, within EX31_RS12000at 64.047 kb on - strand, within EX31_RS12000at 64.047 kb on - strand, within EX31_RS12000at 64.292 kb on - strand, within EX31_RS12000at 64.382 kb on + strand, within EX31_RS12000at 64.382 kb on + strand, within EX31_RS12000at 64.382 kb on + strand, within EX31_RS12000at 64.404 kb on + strand, within EX31_RS12000at 64.404 kb on + strand, within EX31_RS12000at 64.404 kb on + strand, within EX31_RS12000at 64.404 kb on + strand, within EX31_RS12000at 64.404 kb on + strand, within EX31_RS12000at 64.404 kb on + strand, within EX31_RS12000at 64.405 kb on - strand, within EX31_RS12000at 64.405 kb on - strand, within EX31_RS12000at 64.405 kb on - strand, within EX31_RS12000at 64.405 kb on - strand, within EX31_RS12000at 64.405 kb on - strand, within EX31_RS12000at 64.405 kb on - strand, within EX31_RS12000at 64.405 kb on - strand, within EX31_RS12000at 64.405 kb on - strand, within EX31_RS12000at 64.435 kb on + strand, within EX31_RS12000at 64.435 kb on + strand, within EX31_RS12000at 64.435 kb on + strand, within EX31_RS12000at 64.435 kb on + strand, within EX31_RS12000at 64.435 kb on + strand, within EX31_RS12000at 64.435 kb on + strand, within EX31_RS12000at 64.436 kb on - strand, within EX31_RS12000at 64.436 kb on - strand, within EX31_RS12000at 64.436 kb on - strand, within EX31_RS12000at 64.529 kb on + strand, within EX31_RS12000at 64.529 kb on + strand, within EX31_RS12000at 64.530 kb on - strand, within EX31_RS12000at 64.530 kb on - strand, within EX31_RS12000at 64.530 kb on - strand, within EX31_RS12000at 64.607 kb on + strand, within EX31_RS12000at 64.695 kb on + strandat 64.695 kb on + strandat 64.696 kb on - strandat 64.795 kb on - strandat 64.838 kb on + strandat 64.838 kb on + strandat 64.902 kb on + strandat 64.906 kb on + strandat 64.906 kb on + strandat 64.906 kb on + strandat 64.907 kb on - strandat 64.907 kb on - strandat 64.907 kb on - strandat 65.063 kb on + strand, within EX31_RS12005at 65.064 kb on - strand, within EX31_RS12005at 65.124 kb on + strand, within EX31_RS12005at 65.124 kb on + strand, within EX31_RS12005at 65.131 kb on + strand, within EX31_RS12005at 65.140 kb on + strand, within EX31_RS12005at 65.140 kb on + strand, within EX31_RS12005at 65.141 kb on - strand, within EX31_RS12005at 65.141 kb on - strand, within EX31_RS12005at 65.141 kb on - strand, within EX31_RS12005at 65.141 kb on - strand, within EX31_RS12005at 65.182 kb on - strand, within EX31_RS12005at 65.182 kb on - strand, within EX31_RS12005at 65.390 kb on - strand, within EX31_RS12005at 65.469 kb on - strand, within EX31_RS12005at 65.469 kb on - strand, within EX31_RS12005at 65.661 kb on + strand, within EX31_RS12005at 65.661 kb on + strand, within EX31_RS12005at 65.661 kb on + strand, within EX31_RS12005at 65.662 kb on - strand, within EX31_RS12005at 65.662 kb on - strand, within EX31_RS12005at 65.662 kb on - strand, within EX31_RS12005at 65.662 kb on - strand, within EX31_RS12005at 65.683 kb on + strandat 65.684 kb on - strandat 65.716 kb on + strandat 65.716 kb on + strandat 65.717 kb on - strandat 65.799 kb on - strandat 65.807 kb on - strandat 65.807 kb on - strandat 65.872 kb on - strandat 65.885 kb on + strandat 65.995 kb on + strandat 65.997 kb on + strandat 66.006 kb on - strandat 66.008 kb on - strandat 66.008 kb on - strandat 66.009 kb on + strandat 66.012 kb on + strandat 66.101 kb on - strand, within EX31_RS12010at 66.128 kb on - strand, within EX31_RS12010at 66.169 kb on + strand, within EX31_RS12010at 66.170 kb on - strand, within EX31_RS12010at 66.170 kb on - strand, within EX31_RS12010at 66.208 kb on + strand, within EX31_RS12010at 66.208 kb on + strand, within EX31_RS12010at 66.209 kb on - strand, within EX31_RS12010at 66.209 kb on - strand, within EX31_RS12010at 66.228 kb on + strand, within EX31_RS12010at 66.228 kb on + strand, within EX31_RS12010at 66.467 kb on + strand, within EX31_RS12010at 66.467 kb on + strand, within EX31_RS12010at 66.467 kb on + strand, within EX31_RS12010at 66.547 kb on - strand, within EX31_RS12010at 66.609 kb on + strandat 66.716 kb on + strandat 66.716 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking
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63,902 + EX31_RS12000 0.37 -0.2
63,902 + EX31_RS12000 0.37 +0.0
63,902 + EX31_RS12000 0.37 -0.4
63,902 + EX31_RS12000 0.37 +0.9
63,902 + EX31_RS12000 0.37 +0.1
63,902 + EX31_RS12000 0.37 -0.5
63,902 + EX31_RS12000 0.37 -1.4
63,903 - EX31_RS12000 0.37 +0.6
63,903 - EX31_RS12000 0.37 +1.0
63,903 - EX31_RS12000 0.37 -0.4
63,903 - EX31_RS12000 0.37 +0.1
63,903 - EX31_RS12000 0.37 -0.8
63,903 - EX31_RS12000 0.37 -0.2
63,949 - EX31_RS12000 0.40 +0.7
63,949 - EX31_RS12000 0.40 +0.8
63,949 - EX31_RS12000 0.40 -0.0
63,949 - EX31_RS12000 0.40 +0.4
63,961 - EX31_RS12000 0.41 +0.8
64,046 + EX31_RS12000 0.47 +0.3
64,046 + EX31_RS12000 0.47 +0.4
64,046 + EX31_RS12000 0.47 -0.3
64,046 + EX31_RS12000 0.47 -0.2
64,046 + EX31_RS12000 0.47 +0.0
64,046 + EX31_RS12000 0.47 -0.4
64,047 - EX31_RS12000 0.47 +0.5
64,047 - EX31_RS12000 0.47 -0.1
64,047 - EX31_RS12000 0.47 -0.1
64,292 - EX31_RS12000 0.64 +1.1
64,382 + EX31_RS12000 0.70 +0.5
64,382 + EX31_RS12000 0.70 +1.1
64,382 + EX31_RS12000 0.70 -0.7
64,404 + EX31_RS12000 0.72 -0.2
64,404 + EX31_RS12000 0.72 -0.2
64,404 + EX31_RS12000 0.72 -0.0
64,404 + EX31_RS12000 0.72 +0.1
64,404 + EX31_RS12000 0.72 +0.3
64,404 + EX31_RS12000 0.72 -0.4
64,405 - EX31_RS12000 0.72 -0.2
64,405 - EX31_RS12000 0.72 -0.2
64,405 - EX31_RS12000 0.72 +0.7
64,405 - EX31_RS12000 0.72 +0.2
64,405 - EX31_RS12000 0.72 +0.0
64,405 - EX31_RS12000 0.72 +2.0
64,405 - EX31_RS12000 0.72 -0.1
64,405 - EX31_RS12000 0.72 +0.9
64,435 + EX31_RS12000 0.74 -0.1
64,435 + EX31_RS12000 0.74 -0.9
64,435 + EX31_RS12000 0.74 +0.9
64,435 + EX31_RS12000 0.74 -1.0
64,435 + EX31_RS12000 0.74 +0.1
64,435 + EX31_RS12000 0.74 +0.1
64,436 - EX31_RS12000 0.74 +0.1
64,436 - EX31_RS12000 0.74 -1.0
64,436 - EX31_RS12000 0.74 +0.8
64,529 + EX31_RS12000 0.81 +0.8
64,529 + EX31_RS12000 0.81 +0.1
64,530 - EX31_RS12000 0.81 +0.1
64,530 - EX31_RS12000 0.81 +0.3
64,530 - EX31_RS12000 0.81 +0.2
64,607 + EX31_RS12000 0.86 -0.6
64,695 + +0.0
64,695 + +1.7
64,696 - +0.3
64,795 - -0.2
64,838 + +0.2
64,838 + -0.4
64,902 + +0.8
64,906 + -0.6
64,906 + +0.7
64,906 + -1.8
64,907 - -0.5
64,907 - +0.6
64,907 - +0.5
65,063 + EX31_RS12005 0.19 -0.3
65,064 - EX31_RS12005 0.19 -0.0
65,124 + EX31_RS12005 0.26 -0.8
65,124 + EX31_RS12005 0.26 -0.1
65,131 + EX31_RS12005 0.27 -2.5
65,140 + EX31_RS12005 0.28 +1.1
65,140 + EX31_RS12005 0.28 -0.2
65,141 - EX31_RS12005 0.28 -0.9
65,141 - EX31_RS12005 0.28 -0.0
65,141 - EX31_RS12005 0.28 -0.4
65,141 - EX31_RS12005 0.28 -0.5
65,182 - EX31_RS12005 0.33 +0.5
65,182 - EX31_RS12005 0.33 +0.2
65,390 - EX31_RS12005 0.57 -0.6
65,469 - EX31_RS12005 0.66 +0.1
65,469 - EX31_RS12005 0.66 +0.4
65,661 + EX31_RS12005 0.89 -0.7
65,661 + EX31_RS12005 0.89 -0.3
65,661 + EX31_RS12005 0.89 -0.0
65,662 - EX31_RS12005 0.89 -0.5
65,662 - EX31_RS12005 0.89 -0.5
65,662 - EX31_RS12005 0.89 -1.0
65,662 - EX31_RS12005 0.89 +0.3
65,683 + +0.4
65,684 - -0.6
65,716 + +1.6
65,716 + +0.4
65,717 - -0.2
65,799 - -0.4
65,807 - -0.5
65,807 - -0.5
65,872 - -0.0
65,885 + +0.3
65,995 + +1.2
65,997 + +0.4
66,006 - +0.9
66,008 - +0.6
66,008 - -0.4
66,009 + -0.3
66,012 + +0.2
66,101 - EX31_RS12010 0.20 +0.4
66,128 - EX31_RS12010 0.24 -0.9
66,169 + EX31_RS12010 0.30 -0.1
66,170 - EX31_RS12010 0.30 -0.4
66,170 - EX31_RS12010 0.30 -0.4
66,208 + EX31_RS12010 0.36 +0.3
66,208 + EX31_RS12010 0.36 +0.1
66,209 - EX31_RS12010 0.36 -0.5
66,209 - EX31_RS12010 0.36 +0.3
66,228 + EX31_RS12010 0.39 +0.2
66,228 + EX31_RS12010 0.39 -0.5
66,467 + EX31_RS12010 0.73 +1.1
66,467 + EX31_RS12010 0.73 -0.0
66,467 + EX31_RS12010 0.73 +0.6
66,547 - EX31_RS12010 0.85 +0.1
66,609 + +1.5
66,716 + +0.8
66,716 + +0.1

Or see this region's nucleotide sequence