Experiment: L-Glutamine (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS08540 and EX31_RS08545 are separated by 24 nucleotides EX31_RS08545 and EX31_RS08550 are separated by 23 nucleotides EX31_RS08550 and EX31_RS08555 are separated by 216 nucleotides EX31_RS08555 and EX31_RS08560 are separated by 59 nucleotides EX31_RS08560 and EX31_RS08565 are separated by 84 nucleotides
EX31_RS08540: EX31_RS08540 - translation initiation factor IF-2, at 214,385 to 217,078
_RS08540
EX31_RS08545: EX31_RS08545 - transcription termination/antitermination protein NusA, at 217,103 to 218,611
_RS08545
EX31_RS08550: EX31_RS08550 - ribosome maturation factor RimP, at 218,635 to 219,087
_RS08550
EX31_RS08555: EX31_RS08555 - tRNA-Met, at 219,304 to 219,380
_RS08555
EX31_RS08560: EX31_RS08560 - tRNA-Leu, at 219,440 to 219,526
_RS08560
EX31_RS08565: EX31_RS08565 - preprotein translocase subunit SecG, at 219,611 to 219,946
_RS08565
Position (kb) 217
218
219 Strain fitness (log2 ratio) -2
-1
0
1
2 at 217.102 kb on - strand at 217.105 kb on - strand at 217.105 kb on - strand at 217.130 kb on - strand at 217.130 kb on - strand at 217.130 kb on - strand at 217.148 kb on - strand at 217.148 kb on - strand at 217.148 kb on - strand at 217.148 kb on - strand at 217.148 kb on - strand at 217.148 kb on - strand at 217.229 kb on - strand at 217.229 kb on - strand at 217.229 kb on - strand at 217.229 kb on - strand at 217.229 kb on - strand at 217.253 kb on - strand at 217.253 kb on - strand at 217.253 kb on - strand at 217.253 kb on - strand at 217.265 kb on - strand, within EX31_RS08545 at 217.265 kb on - strand, within EX31_RS08545 at 217.265 kb on - strand, within EX31_RS08545 at 217.286 kb on - strand, within EX31_RS08545 at 217.286 kb on - strand, within EX31_RS08545 at 217.286 kb on - strand, within EX31_RS08545 at 217.335 kb on - strand, within EX31_RS08545 at 217.335 kb on - strand, within EX31_RS08545 at 217.416 kb on - strand, within EX31_RS08545 at 217.416 kb on - strand, within EX31_RS08545 at 217.424 kb on - strand, within EX31_RS08545 at 217.435 kb on - strand, within EX31_RS08545 at 217.435 kb on - strand, within EX31_RS08545 at 217.435 kb on - strand, within EX31_RS08545 at 217.435 kb on - strand, within EX31_RS08545 at 217.435 kb on - strand, within EX31_RS08545 at 217.507 kb on - strand, within EX31_RS08545 at 217.520 kb on - strand, within EX31_RS08545 at 217.520 kb on - strand, within EX31_RS08545 at 218.637 kb on - strand at 218.973 kb on - strand, within EX31_RS08550 at 218.973 kb on - strand, within EX31_RS08550 at 218.973 kb on - strand, within EX31_RS08550 at 219.077 kb on - strand at 219.288 kb on - strand at 219.399 kb on - strand at 219.402 kb on - strand at 219.429 kb on - strand at 219.429 kb on - strand at 219.429 kb on - strand at 219.429 kb on - strand at 219.429 kb on - strand at 219.550 kb on - strand at 219.552 kb on - strand at 219.552 kb on - strand at 219.552 kb on - strand at 219.552 kb on - strand at 219.552 kb on - strand at 219.552 kb on - strand at 219.552 kb on - strand at 219.552 kb on - strand at 219.552 kb on - strand at 219.565 kb on - strand at 219.565 kb on - strand at 219.565 kb on - strand at 219.585 kb on - strand at 219.585 kb on - strand at 219.597 kb on - strand at 219.606 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; shaking remove 217,102 - +1.0 217,105 - -0.7 217,105 - +1.1 217,130 - +2.6 217,130 - +0.9 217,130 - +0.5 217,148 - +0.4 217,148 - -0.9 217,148 - +0.6 217,148 - +1.9 217,148 - +0.0 217,148 - -0.6 217,229 - -1.8 217,229 - -0.9 217,229 - +0.2 217,229 - -0.4 217,229 - +1.1 217,253 - +0.7 217,253 - +1.3 217,253 - +0.3 217,253 - -0.4 217,265 - EX31_RS08545 0.11 -0.2 217,265 - EX31_RS08545 0.11 +0.2 217,265 - EX31_RS08545 0.11 -0.4 217,286 - EX31_RS08545 0.12 +0.5 217,286 - EX31_RS08545 0.12 +0.4 217,286 - EX31_RS08545 0.12 -0.7 217,335 - EX31_RS08545 0.15 -0.6 217,335 - EX31_RS08545 0.15 +0.2 217,416 - EX31_RS08545 0.21 -0.3 217,416 - EX31_RS08545 0.21 +0.8 217,424 - EX31_RS08545 0.21 -1.7 217,435 - EX31_RS08545 0.22 -1.0 217,435 - EX31_RS08545 0.22 -0.7 217,435 - EX31_RS08545 0.22 -1.0 217,435 - EX31_RS08545 0.22 -0.5 217,435 - EX31_RS08545 0.22 -0.4 217,507 - EX31_RS08545 0.27 -0.3 217,520 - EX31_RS08545 0.28 +1.3 217,520 - EX31_RS08545 0.28 +0.4 218,637 - -0.0 218,973 - EX31_RS08550 0.75 -0.8 218,973 - EX31_RS08550 0.75 +0.1 218,973 - EX31_RS08550 0.75 -0.9 219,077 - -1.5 219,288 - +1.1 219,399 - +0.4 219,402 - -0.3 219,429 - -0.3 219,429 - -0.4 219,429 - -0.9 219,429 - +0.7 219,429 - +0.1 219,550 - +0.2 219,552 - +0.7 219,552 - -0.1 219,552 - +0.2 219,552 - -0.1 219,552 - +0.4 219,552 - +0.1 219,552 - -0.3 219,552 - -0.5 219,552 - +0.8 219,565 - -1.3 219,565 - -0.0 219,565 - -0.3 219,585 - +0.6 219,585 - +1.0 219,597 - +0.3 219,606 - +0.3
Or see this region's nucleotide sequence