Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS32295 and AZOBR_RS32300 are separated by 14 nucleotides AZOBR_RS32300 and AZOBR_RS32305 are separated by 27 nucleotides AZOBR_RS32305 and AZOBR_RS32310 overlap by 4 nucleotides AZOBR_RS32310 and AZOBR_RS32315 are separated by 157 nucleotides
AZOBR_RS32295: AZOBR_RS32295 - Asp/Glu racemase, at 613,572 to 614,327
_RS32295
AZOBR_RS32300: AZOBR_RS32300 - alpha/beta hydrolase, at 614,342 to 615,151
_RS32300
AZOBR_RS32305: AZOBR_RS32305 - hypothetical protein, at 615,179 to 616,225
_RS32305
AZOBR_RS32310: AZOBR_RS32310 - N-carbamoylsarcosine amidase, at 616,222 to 616,836
_RS32310
AZOBR_RS32315: AZOBR_RS32315 - TetR family transcriptional regulator, at 616,994 to 617,692
_RS32315
Position (kb) 615
616
617 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 614.253 kb on + strand at 614.254 kb on - strand at 614.254 kb on - strand at 614.322 kb on + strand at 614.322 kb on + strand at 614.323 kb on - strand at 614.336 kb on + strand at 614.336 kb on + strand at 614.337 kb on - strand at 614.402 kb on + strand at 614.402 kb on + strand at 614.402 kb on + strand at 614.403 kb on - strand at 614.403 kb on - strand at 614.513 kb on + strand, within AZOBR_RS32300 at 614.513 kb on + strand, within AZOBR_RS32300 at 614.513 kb on + strand, within AZOBR_RS32300 at 614.514 kb on - strand, within AZOBR_RS32300 at 614.514 kb on - strand, within AZOBR_RS32300 at 614.514 kb on - strand, within AZOBR_RS32300 at 614.514 kb on - strand, within AZOBR_RS32300 at 614.514 kb on - strand, within AZOBR_RS32300 at 614.630 kb on + strand, within AZOBR_RS32300 at 614.630 kb on + strand, within AZOBR_RS32300 at 614.775 kb on - strand, within AZOBR_RS32300 at 615.113 kb on + strand at 615.113 kb on + strand at 615.113 kb on + strand at 615.114 kb on - strand at 615.114 kb on - strand at 615.199 kb on + strand at 615.710 kb on + strand, within AZOBR_RS32305 at 615.711 kb on - strand, within AZOBR_RS32305 at 615.842 kb on + strand, within AZOBR_RS32305 at 615.842 kb on + strand, within AZOBR_RS32305 at 615.842 kb on + strand, within AZOBR_RS32305 at 615.843 kb on - strand, within AZOBR_RS32305 at 615.929 kb on + strand, within AZOBR_RS32305 at 615.930 kb on - strand, within AZOBR_RS32305 at 615.930 kb on - strand, within AZOBR_RS32305 at 615.930 kb on - strand, within AZOBR_RS32305 at 615.960 kb on - strand, within AZOBR_RS32305 at 616.247 kb on - strand at 616.247 kb on - strand at 616.247 kb on - strand at 616.795 kb on + strand at 617.100 kb on - strand, within AZOBR_RS32315 at 617.100 kb on - strand, within AZOBR_RS32315 at 617.166 kb on - strand, within AZOBR_RS32315
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 614,253 + -1.3 614,254 - -3.0 614,254 - +1.4 614,322 + -1.9 614,322 + +1.0 614,323 - -1.7 614,336 + -2.6 614,336 + +0.1 614,337 - +0.1 614,402 + -0.7 614,402 + +1.4 614,402 + +0.0 614,403 - -0.4 614,403 - -2.4 614,513 + AZOBR_RS32300 0.21 +0.3 614,513 + AZOBR_RS32300 0.21 +1.7 614,513 + AZOBR_RS32300 0.21 -0.5 614,514 - AZOBR_RS32300 0.21 -0.6 614,514 - AZOBR_RS32300 0.21 +1.7 614,514 - AZOBR_RS32300 0.21 +1.7 614,514 - AZOBR_RS32300 0.21 +0.7 614,514 - AZOBR_RS32300 0.21 +0.9 614,630 + AZOBR_RS32300 0.36 +0.1 614,630 + AZOBR_RS32300 0.36 +0.4 614,775 - AZOBR_RS32300 0.53 -0.2 615,113 + -0.9 615,113 + -2.9 615,113 + -0.0 615,114 - -5.1 615,114 - +0.9 615,199 + -0.5 615,710 + AZOBR_RS32305 0.51 -1.0 615,711 - AZOBR_RS32305 0.51 +0.6 615,842 + AZOBR_RS32305 0.63 -0.7 615,842 + AZOBR_RS32305 0.63 +1.5 615,842 + AZOBR_RS32305 0.63 +0.4 615,843 - AZOBR_RS32305 0.63 +0.7 615,929 + AZOBR_RS32305 0.72 +2.1 615,930 - AZOBR_RS32305 0.72 +2.2 615,930 - AZOBR_RS32305 0.72 -0.9 615,930 - AZOBR_RS32305 0.72 -1.0 615,960 - AZOBR_RS32305 0.75 +0.2 616,247 - +1.5 616,247 - -0.0 616,247 - -0.3 616,795 + +0.0 617,100 - AZOBR_RS32315 0.15 -3.4 617,100 - AZOBR_RS32315 0.15 -3.6 617,166 - AZOBR_RS32315 0.25 -1.7
Or see this region's nucleotide sequence