Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS32190 and AZOBR_RS32195 are separated by 91 nucleotides AZOBR_RS32195 and AZOBR_RS32200 are separated by 96 nucleotides AZOBR_RS32200 and AZOBR_RS32205 are separated by 125 nucleotides AZOBR_RS32205 and AZOBR_RS32210 overlap by 4 nucleotides AZOBR_RS32210 and AZOBR_RS32215 are separated by 304 nucleotides AZOBR_RS32215 and AZOBR_RS32220 are separated by 22 nucleotides
AZOBR_RS32190: AZOBR_RS32190 - glycerol-3-phosphate dehydrogenase subunit C, at 589,497 to 589,766
_RS32190
AZOBR_RS32195: AZOBR_RS32195 - glycerol-3-phosphate dehydrogenase subunit C, at 589,858 to 590,124
_RS32195
AZOBR_RS32200: AZOBR_RS32200 - glycerol-3-phosphate dehydrogenase subunit C, at 590,221 to 590,493
_RS32200
AZOBR_RS32205: AZOBR_RS32205 - hypothetical protein, at 590,619 to 590,852
_RS32205
AZOBR_RS32210: AZOBR_RS32210 - hypothetical protein, at 590,849 to 591,100
_RS32210
AZOBR_RS32215: AZOBR_RS32215 - hypothetical protein, at 591,405 to 591,755
_RS32215
AZOBR_RS32220: AZOBR_RS32220 - hypothetical protein, at 591,778 to 592,857
_RS32220
Position (kb)
590
591 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 589.665 kb on + strand, within AZOBR_RS32190 at 589.666 kb on - strand, within AZOBR_RS32190 at 589.666 kb on - strand, within AZOBR_RS32190 at 589.666 kb on - strand, within AZOBR_RS32190 at 589.849 kb on + strand at 589.960 kb on + strand, within AZOBR_RS32195 at 589.961 kb on - strand, within AZOBR_RS32195 at 590.026 kb on + strand, within AZOBR_RS32195 at 590.026 kb on + strand, within AZOBR_RS32195 at 590.027 kb on - strand, within AZOBR_RS32195 at 590.027 kb on - strand, within AZOBR_RS32195 at 590.027 kb on - strand, within AZOBR_RS32195 at 590.072 kb on - strand, within AZOBR_RS32195 at 590.206 kb on + strand at 590.206 kb on + strand at 590.206 kb on + strand at 590.206 kb on + strand at 590.206 kb on + strand at 590.206 kb on + strand at 590.207 kb on - strand at 590.389 kb on + strand, within AZOBR_RS32200 at 590.389 kb on + strand, within AZOBR_RS32200 at 590.389 kb on + strand, within AZOBR_RS32200 at 590.389 kb on + strand, within AZOBR_RS32200 at 590.390 kb on - strand, within AZOBR_RS32200 at 590.491 kb on + strand at 590.491 kb on + strand at 590.492 kb on - strand at 590.566 kb on - strand at 590.566 kb on - strand at 590.715 kb on + strand, within AZOBR_RS32205 at 590.715 kb on + strand, within AZOBR_RS32205 at 590.715 kb on + strand, within AZOBR_RS32205 at 590.715 kb on + strand, within AZOBR_RS32205 at 590.715 kb on + strand, within AZOBR_RS32205 at 590.715 kb on + strand, within AZOBR_RS32205 at 590.716 kb on - strand, within AZOBR_RS32205 at 590.716 kb on - strand, within AZOBR_RS32205 at 590.716 kb on - strand, within AZOBR_RS32205 at 591.449 kb on - strand, within AZOBR_RS32215 at 591.449 kb on - strand, within AZOBR_RS32215 at 591.489 kb on - strand, within AZOBR_RS32215 at 591.513 kb on + strand, within AZOBR_RS32215 at 591.513 kb on + strand, within AZOBR_RS32215 at 591.513 kb on + strand, within AZOBR_RS32215 at 591.514 kb on - strand, within AZOBR_RS32215 at 591.514 kb on - strand, within AZOBR_RS32215 at 591.645 kb on + strand, within AZOBR_RS32215 at 591.645 kb on + strand, within AZOBR_RS32215 at 591.645 kb on + strand, within AZOBR_RS32215 at 591.646 kb on - strand, within AZOBR_RS32215 at 591.646 kb on - strand, within AZOBR_RS32215 at 591.646 kb on - strand, within AZOBR_RS32215 at 591.646 kb on - strand, within AZOBR_RS32215 at 591.646 kb on - strand, within AZOBR_RS32215 at 591.737 kb on + strand at 591.737 kb on + strand at 591.737 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 589,665 + AZOBR_RS32190 0.62 -0.4 589,666 - AZOBR_RS32190 0.63 -1.5 589,666 - AZOBR_RS32190 0.63 -0.4 589,666 - AZOBR_RS32190 0.63 +0.9 589,849 + -0.9 589,960 + AZOBR_RS32195 0.38 -0.8 589,961 - AZOBR_RS32195 0.39 +0.4 590,026 + AZOBR_RS32195 0.63 +0.1 590,026 + AZOBR_RS32195 0.63 -0.6 590,027 - AZOBR_RS32195 0.63 +0.3 590,027 - AZOBR_RS32195 0.63 -0.3 590,027 - AZOBR_RS32195 0.63 -0.3 590,072 - AZOBR_RS32195 0.80 -0.8 590,206 + +0.4 590,206 + -0.3 590,206 + -0.1 590,206 + +0.1 590,206 + -0.7 590,206 + -1.1 590,207 - +0.8 590,389 + AZOBR_RS32200 0.62 -3.9 590,389 + AZOBR_RS32200 0.62 +0.1 590,389 + AZOBR_RS32200 0.62 +1.2 590,389 + AZOBR_RS32200 0.62 +0.1 590,390 - AZOBR_RS32200 0.62 +0.3 590,491 + +1.7 590,491 + +0.5 590,492 - +1.5 590,566 - +1.1 590,566 - +0.7 590,715 + AZOBR_RS32205 0.41 +1.3 590,715 + AZOBR_RS32205 0.41 -0.7 590,715 + AZOBR_RS32205 0.41 -0.2 590,715 + AZOBR_RS32205 0.41 +1.4 590,715 + AZOBR_RS32205 0.41 +0.6 590,715 + AZOBR_RS32205 0.41 +1.3 590,716 - AZOBR_RS32205 0.41 -0.6 590,716 - AZOBR_RS32205 0.41 +1.0 590,716 - AZOBR_RS32205 0.41 +0.1 591,449 - AZOBR_RS32215 0.13 +1.0 591,449 - AZOBR_RS32215 0.13 -3.4 591,489 - AZOBR_RS32215 0.24 -0.2 591,513 + AZOBR_RS32215 0.31 -4.3 591,513 + AZOBR_RS32215 0.31 +1.4 591,513 + AZOBR_RS32215 0.31 -0.8 591,514 - AZOBR_RS32215 0.31 -3.4 591,514 - AZOBR_RS32215 0.31 +0.6 591,645 + AZOBR_RS32215 0.68 +2.1 591,645 + AZOBR_RS32215 0.68 -0.9 591,645 + AZOBR_RS32215 0.68 -3.0 591,646 - AZOBR_RS32215 0.69 -0.2 591,646 - AZOBR_RS32215 0.69 +0.1 591,646 - AZOBR_RS32215 0.69 +0.3 591,646 - AZOBR_RS32215 0.69 -0.7 591,646 - AZOBR_RS32215 0.69 +0.0 591,737 + -0.2 591,737 + +1.3 591,737 + -0.8
Or see this region's nucleotide sequence