Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS32100 and AZOBR_RS32105 overlap by 4 nucleotides AZOBR_RS32105 and AZOBR_RS32110 are separated by 11 nucleotides AZOBR_RS32110 and AZOBR_RS32115 are separated by 107 nucleotides AZOBR_RS32115 and AZOBR_RS32120 are separated by 391 nucleotides
AZOBR_RS32100: AZOBR_RS32100 - cytochrome C, at 572,893 to 573,513
_RS32100
AZOBR_RS32105: AZOBR_RS32105 - nitrate reductase, at 573,510 to 573,992
_RS32105
AZOBR_RS32110: AZOBR_RS32110 - nitrate reductase catalytic subunit, at 574,004 to 576,514
_RS32110
AZOBR_RS32115: AZOBR_RS32115 - nitrate reductase, at 576,622 to 576,855
_RS32115
AZOBR_RS32120: AZOBR_RS32120 - hypothetical protein, at 577,247 to 577,744
_RS32120
Position (kb)
574
575
576
577 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 573.005 kb on + strand, within AZOBR_RS32100 at 573.006 kb on - strand, within AZOBR_RS32100 at 573.006 kb on - strand, within AZOBR_RS32100 at 573.006 kb on - strand, within AZOBR_RS32100 at 573.006 kb on - strand, within AZOBR_RS32100 at 573.317 kb on + strand, within AZOBR_RS32100 at 573.620 kb on - strand, within AZOBR_RS32105 at 573.761 kb on - strand, within AZOBR_RS32105 at 573.761 kb on - strand, within AZOBR_RS32105 at 574.392 kb on + strand, within AZOBR_RS32110 at 574.393 kb on - strand, within AZOBR_RS32110 at 574.393 kb on - strand, within AZOBR_RS32110 at 574.393 kb on - strand, within AZOBR_RS32110 at 574.435 kb on - strand, within AZOBR_RS32110 at 574.464 kb on + strand, within AZOBR_RS32110 at 574.465 kb on - strand, within AZOBR_RS32110 at 574.545 kb on + strand, within AZOBR_RS32110 at 574.545 kb on + strand, within AZOBR_RS32110 at 574.545 kb on + strand, within AZOBR_RS32110 at 574.545 kb on + strand, within AZOBR_RS32110 at 574.546 kb on - strand, within AZOBR_RS32110 at 574.546 kb on - strand, within AZOBR_RS32110 at 574.546 kb on - strand, within AZOBR_RS32110 at 574.546 kb on - strand, within AZOBR_RS32110 at 574.555 kb on - strand, within AZOBR_RS32110 at 574.597 kb on - strand, within AZOBR_RS32110 at 574.623 kb on + strand, within AZOBR_RS32110 at 574.623 kb on + strand, within AZOBR_RS32110 at 574.624 kb on - strand, within AZOBR_RS32110 at 574.624 kb on - strand, within AZOBR_RS32110 at 574.624 kb on - strand, within AZOBR_RS32110 at 574.624 kb on - strand, within AZOBR_RS32110 at 574.657 kb on - strand, within AZOBR_RS32110 at 574.707 kb on + strand, within AZOBR_RS32110 at 574.707 kb on + strand, within AZOBR_RS32110 at 574.708 kb on - strand, within AZOBR_RS32110 at 574.708 kb on - strand, within AZOBR_RS32110 at 574.708 kb on - strand, within AZOBR_RS32110 at 574.708 kb on - strand, within AZOBR_RS32110 at 574.963 kb on - strand, within AZOBR_RS32110 at 575.010 kb on + strand, within AZOBR_RS32110 at 575.010 kb on + strand, within AZOBR_RS32110 at 575.010 kb on + strand, within AZOBR_RS32110 at 575.011 kb on - strand, within AZOBR_RS32110 at 575.067 kb on + strand, within AZOBR_RS32110 at 575.331 kb on + strand, within AZOBR_RS32110 at 575.331 kb on + strand, within AZOBR_RS32110 at 575.331 kb on + strand, within AZOBR_RS32110 at 575.331 kb on + strand, within AZOBR_RS32110 at 575.332 kb on - strand, within AZOBR_RS32110 at 575.488 kb on - strand, within AZOBR_RS32110 at 575.688 kb on + strand, within AZOBR_RS32110 at 575.689 kb on - strand, within AZOBR_RS32110 at 575.689 kb on - strand, within AZOBR_RS32110 at 575.689 kb on - strand, within AZOBR_RS32110 at 575.689 kb on - strand, within AZOBR_RS32110 at 575.763 kb on + strand, within AZOBR_RS32110 at 575.763 kb on + strand, within AZOBR_RS32110 at 575.763 kb on + strand, within AZOBR_RS32110 at 575.764 kb on - strand, within AZOBR_RS32110 at 575.764 kb on - strand, within AZOBR_RS32110 at 575.764 kb on - strand, within AZOBR_RS32110 at 575.898 kb on + strand, within AZOBR_RS32110 at 575.898 kb on + strand, within AZOBR_RS32110 at 576.028 kb on - strand, within AZOBR_RS32110 at 576.223 kb on - strand, within AZOBR_RS32110 at 576.244 kb on - strand, within AZOBR_RS32110 at 576.244 kb on - strand, within AZOBR_RS32110 at 576.259 kb on + strand, within AZOBR_RS32110 at 576.493 kb on - strand at 576.514 kb on + strand at 576.515 kb on - strand at 576.626 kb on + strand at 576.626 kb on + strand at 576.635 kb on + strand at 576.636 kb on - strand at 576.636 kb on - strand at 576.891 kb on + strand at 577.138 kb on + strand at 577.138 kb on + strand at 577.139 kb on - strand at 577.149 kb on - strand at 577.249 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 573,005 + AZOBR_RS32100 0.18 +2.6 573,006 - AZOBR_RS32100 0.18 -0.2 573,006 - AZOBR_RS32100 0.18 +1.4 573,006 - AZOBR_RS32100 0.18 +1.0 573,006 - AZOBR_RS32100 0.18 +1.4 573,317 + AZOBR_RS32100 0.68 +0.2 573,620 - AZOBR_RS32105 0.23 +0.1 573,761 - AZOBR_RS32105 0.52 -2.0 573,761 - AZOBR_RS32105 0.52 -2.7 574,392 + AZOBR_RS32110 0.15 -3.8 574,393 - AZOBR_RS32110 0.15 -0.2 574,393 - AZOBR_RS32110 0.15 -1.7 574,393 - AZOBR_RS32110 0.15 +0.6 574,435 - AZOBR_RS32110 0.17 -1.7 574,464 + AZOBR_RS32110 0.18 +1.3 574,465 - AZOBR_RS32110 0.18 +0.8 574,545 + AZOBR_RS32110 0.22 +0.8 574,545 + AZOBR_RS32110 0.22 -3.3 574,545 + AZOBR_RS32110 0.22 +0.2 574,545 + AZOBR_RS32110 0.22 -1.2 574,546 - AZOBR_RS32110 0.22 +0.3 574,546 - AZOBR_RS32110 0.22 -1.7 574,546 - AZOBR_RS32110 0.22 +0.4 574,546 - AZOBR_RS32110 0.22 +0.6 574,555 - AZOBR_RS32110 0.22 -0.1 574,597 - AZOBR_RS32110 0.24 +1.2 574,623 + AZOBR_RS32110 0.25 +0.8 574,623 + AZOBR_RS32110 0.25 -0.2 574,624 - AZOBR_RS32110 0.25 -0.3 574,624 - AZOBR_RS32110 0.25 +0.7 574,624 - AZOBR_RS32110 0.25 -1.1 574,624 - AZOBR_RS32110 0.25 +0.6 574,657 - AZOBR_RS32110 0.26 -2.2 574,707 + AZOBR_RS32110 0.28 +0.8 574,707 + AZOBR_RS32110 0.28 +3.2 574,708 - AZOBR_RS32110 0.28 -2.5 574,708 - AZOBR_RS32110 0.28 -2.1 574,708 - AZOBR_RS32110 0.28 -1.7 574,708 - AZOBR_RS32110 0.28 -1.3 574,963 - AZOBR_RS32110 0.38 -0.2 575,010 + AZOBR_RS32110 0.40 -0.5 575,010 + AZOBR_RS32110 0.40 -3.4 575,010 + AZOBR_RS32110 0.40 -0.1 575,011 - AZOBR_RS32110 0.40 -0.4 575,067 + AZOBR_RS32110 0.42 -1.9 575,331 + AZOBR_RS32110 0.53 -0.1 575,331 + AZOBR_RS32110 0.53 -1.0 575,331 + AZOBR_RS32110 0.53 +0.3 575,331 + AZOBR_RS32110 0.53 -0.4 575,332 - AZOBR_RS32110 0.53 +0.3 575,488 - AZOBR_RS32110 0.59 -0.3 575,688 + AZOBR_RS32110 0.67 -0.5 575,689 - AZOBR_RS32110 0.67 -4.4 575,689 - AZOBR_RS32110 0.67 -0.2 575,689 - AZOBR_RS32110 0.67 -0.6 575,689 - AZOBR_RS32110 0.67 -0.4 575,763 + AZOBR_RS32110 0.70 -0.1 575,763 + AZOBR_RS32110 0.70 +0.3 575,763 + AZOBR_RS32110 0.70 -0.2 575,764 - AZOBR_RS32110 0.70 -2.1 575,764 - AZOBR_RS32110 0.70 -2.3 575,764 - AZOBR_RS32110 0.70 -3.4 575,898 + AZOBR_RS32110 0.75 +1.1 575,898 + AZOBR_RS32110 0.75 +0.4 576,028 - AZOBR_RS32110 0.81 +0.2 576,223 - AZOBR_RS32110 0.88 +1.1 576,244 - AZOBR_RS32110 0.89 -0.1 576,244 - AZOBR_RS32110 0.89 +1.1 576,259 + AZOBR_RS32110 0.90 -0.1 576,493 - -0.4 576,514 + -2.2 576,515 - -3.8 576,626 + +0.6 576,626 + +0.7 576,635 + -1.3 576,636 - -2.2 576,636 - +0.7 576,891 + -1.6 577,138 + +0.1 577,138 + +0.1 577,139 - -3.1 577,149 - +0.2 577,249 - -1.0
Or see this region's nucleotide sequence