Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS31950 and AZOBR_RS31955 are separated by 340 nucleotides AZOBR_RS31955 and AZOBR_RS31960 overlap by 4 nucleotides AZOBR_RS31960 and AZOBR_RS31965 are separated by 192 nucleotides
AZOBR_RS31950: AZOBR_RS31950 - GntR family transcriptional regulator, at 534,967 to 535,656
_RS31950
AZOBR_RS31955: AZOBR_RS31955 - lipid kinase, at 535,997 to 536,881
_RS31955
AZOBR_RS31960: AZOBR_RS31960 - HAD family hydrolase, at 536,878 to 537,555
_RS31960
AZOBR_RS31965: AZOBR_RS31965 - membrane protein, at 537,748 to 538,887
_RS31965
Position (kb) 535
536
537 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 535.553 kb on + strand, within AZOBR_RS31950 at 535.553 kb on + strand, within AZOBR_RS31950 at 535.554 kb on - strand, within AZOBR_RS31950 at 535.554 kb on - strand, within AZOBR_RS31950 at 535.554 kb on - strand, within AZOBR_RS31950 at 535.554 kb on - strand, within AZOBR_RS31950 at 535.593 kb on - strand at 535.593 kb on - strand at 536.012 kb on + strand at 536.012 kb on + strand at 536.012 kb on + strand at 536.013 kb on - strand at 536.013 kb on - strand at 536.223 kb on + strand, within AZOBR_RS31955 at 536.224 kb on - strand, within AZOBR_RS31955 at 536.933 kb on + strand at 536.933 kb on + strand at 536.934 kb on - strand at 537.126 kb on - strand, within AZOBR_RS31960 at 537.494 kb on + strand at 537.495 kb on - strand at 537.495 kb on - strand at 537.495 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 535,553 + AZOBR_RS31950 0.85 +0.9 535,553 + AZOBR_RS31950 0.85 -2.6 535,554 - AZOBR_RS31950 0.85 -3.0 535,554 - AZOBR_RS31950 0.85 +0.1 535,554 - AZOBR_RS31950 0.85 +1.3 535,554 - AZOBR_RS31950 0.85 +0.2 535,593 - +0.4 535,593 - +1.6 536,012 + +0.0 536,012 + +0.7 536,012 + -1.2 536,013 - +0.1 536,013 - +0.5 536,223 + AZOBR_RS31955 0.26 -3.9 536,224 - AZOBR_RS31955 0.26 -0.4 536,933 + +2.6 536,933 + +0.0 536,934 - -1.0 537,126 - AZOBR_RS31960 0.37 +0.5 537,494 + -0.3 537,495 - +0.3 537,495 - -2.3 537,495 - +0.7
Or see this region's nucleotide sequence