Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS31125 and AZOBR_RS31130 are separated by 79 nucleotides AZOBR_RS31130 and AZOBR_RS31135 are separated by 279 nucleotides
AZOBR_RS31125: AZOBR_RS31125 - biotin synthase, at 347,194 to 348,117
_RS31125
AZOBR_RS31130: AZOBR_RS31130 - membrane protein, at 348,197 to 348,904
_RS31130
AZOBR_RS31135: AZOBR_RS31135 - pyruvate phosphate dikinase, at 349,184 to 351,877
_RS31135
Position (kb)
348
349 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 347.383 kb on + strand, within AZOBR_RS31125 at 347.632 kb on + strand, within AZOBR_RS31125 at 347.632 kb on + strand, within AZOBR_RS31125 at 347.633 kb on - strand, within AZOBR_RS31125 at 347.633 kb on - strand, within AZOBR_RS31125 at 347.635 kb on + strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.668 kb on + strand, within AZOBR_RS31125 at 347.669 kb on - strand, within AZOBR_RS31125 at 347.669 kb on - strand, within AZOBR_RS31125 at 347.959 kb on + strand, within AZOBR_RS31125 at 348.043 kb on + strand at 348.044 kb on - strand at 348.044 kb on - strand at 348.233 kb on + strand at 348.233 kb on + strand at 348.234 kb on - strand at 348.234 kb on - strand at 348.234 kb on - strand at 348.269 kb on + strand, within AZOBR_RS31130 at 348.270 kb on - strand, within AZOBR_RS31130 at 348.270 kb on - strand, within AZOBR_RS31130 at 348.434 kb on + strand, within AZOBR_RS31130 at 348.575 kb on + strand, within AZOBR_RS31130 at 348.575 kb on + strand, within AZOBR_RS31130 at 348.575 kb on + strand, within AZOBR_RS31130 at 348.602 kb on + strand, within AZOBR_RS31130 at 348.603 kb on - strand, within AZOBR_RS31130 at 348.603 kb on - strand, within AZOBR_RS31130 at 348.638 kb on + strand, within AZOBR_RS31130 at 348.639 kb on - strand, within AZOBR_RS31130 at 348.639 kb on - strand, within AZOBR_RS31130 at 348.671 kb on + strand, within AZOBR_RS31130 at 348.671 kb on + strand, within AZOBR_RS31130 at 348.672 kb on - strand, within AZOBR_RS31130 at 348.672 kb on - strand, within AZOBR_RS31130 at 348.987 kb on - strand at 348.987 kb on - strand at 348.987 kb on - strand at 349.017 kb on - strand at 349.161 kb on + strand at 349.161 kb on + strand at 349.214 kb on + strand at 349.355 kb on + strand at 349.355 kb on + strand at 349.356 kb on - strand at 349.362 kb on - strand at 349.379 kb on + strand at 349.757 kb on + strand, within AZOBR_RS31135 at 349.874 kb on + strand, within AZOBR_RS31135 at 349.875 kb on - strand, within AZOBR_RS31135
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 347,383 + AZOBR_RS31125 0.20 -3.2 347,632 + AZOBR_RS31125 0.47 -2.6 347,632 + AZOBR_RS31125 0.47 +1.0 347,633 - AZOBR_RS31125 0.48 +0.1 347,633 - AZOBR_RS31125 0.48 -4.9 347,635 + AZOBR_RS31125 0.48 +0.4 347,636 - AZOBR_RS31125 0.48 -0.1 347,636 - AZOBR_RS31125 0.48 +0.2 347,636 - AZOBR_RS31125 0.48 -1.7 347,636 - AZOBR_RS31125 0.48 -0.6 347,636 - AZOBR_RS31125 0.48 -0.4 347,636 - AZOBR_RS31125 0.48 -3.2 347,668 + AZOBR_RS31125 0.51 -0.3 347,669 - AZOBR_RS31125 0.51 +1.4 347,669 - AZOBR_RS31125 0.51 -1.1 347,959 + AZOBR_RS31125 0.83 -1.0 348,043 + +0.0 348,044 - -0.1 348,044 - -0.6 348,233 + +0.1 348,233 + +0.2 348,234 - +1.0 348,234 - +0.5 348,234 - -0.3 348,269 + AZOBR_RS31130 0.10 +0.2 348,270 - AZOBR_RS31130 0.10 +3.2 348,270 - AZOBR_RS31130 0.10 -0.4 348,434 + AZOBR_RS31130 0.33 +0.6 348,575 + AZOBR_RS31130 0.53 -1.5 348,575 + AZOBR_RS31130 0.53 -0.1 348,575 + AZOBR_RS31130 0.53 +0.7 348,602 + AZOBR_RS31130 0.57 -2.9 348,603 - AZOBR_RS31130 0.57 +0.5 348,603 - AZOBR_RS31130 0.57 +0.3 348,638 + AZOBR_RS31130 0.62 -1.2 348,639 - AZOBR_RS31130 0.62 +0.3 348,639 - AZOBR_RS31130 0.62 +0.5 348,671 + AZOBR_RS31130 0.67 -1.0 348,671 + AZOBR_RS31130 0.67 +0.3 348,672 - AZOBR_RS31130 0.67 +0.6 348,672 - AZOBR_RS31130 0.67 -0.1 348,987 - -1.0 348,987 - -3.3 348,987 - +0.4 349,017 - -0.3 349,161 + +0.8 349,161 + +0.2 349,214 + +0.2 349,355 + +1.4 349,355 + -1.6 349,356 - +1.9 349,362 - -1.4 349,379 + +2.4 349,757 + AZOBR_RS31135 0.21 +0.1 349,874 + AZOBR_RS31135 0.26 -3.4 349,875 - AZOBR_RS31135 0.26 -1.8
Or see this region's nucleotide sequence