Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS29180 and AZOBR_RS29185 are separated by 63 nucleotides AZOBR_RS29185 and AZOBR_RS29190 are separated by 86 nucleotides
AZOBR_RS29180: AZOBR_RS29180 - CoA-transferase, at 672,419 to 673,660
_RS29180
AZOBR_RS29185: AZOBR_RS29185 - aldehyde dehydrogenase, at 673,724 to 675,160
_RS29185
AZOBR_RS29190: AZOBR_RS29190 - hydroxyacid dehydrogenase, at 675,247 to 676,149
_RS29190
Position (kb) 673
674
675
676 Strain fitness (log2 ratio) -2
-1
0
1
2 at 672.800 kb on + strand, within AZOBR_RS29180 at 672.801 kb on - strand, within AZOBR_RS29180 at 672.836 kb on + strand, within AZOBR_RS29180 at 672.836 kb on + strand, within AZOBR_RS29180 at 672.836 kb on + strand, within AZOBR_RS29180 at 672.854 kb on + strand, within AZOBR_RS29180 at 672.974 kb on + strand, within AZOBR_RS29180 at 672.974 kb on + strand, within AZOBR_RS29180 at 672.975 kb on - strand, within AZOBR_RS29180 at 673.532 kb on + strand, within AZOBR_RS29180 at 673.823 kb on + strand at 673.824 kb on - strand at 674.051 kb on + strand, within AZOBR_RS29185 at 674.204 kb on + strand, within AZOBR_RS29185 at 674.205 kb on - strand, within AZOBR_RS29185 at 674.789 kb on + strand, within AZOBR_RS29185 at 674.789 kb on + strand, within AZOBR_RS29185 at 674.790 kb on - strand, within AZOBR_RS29185 at 675.038 kb on + strand at 675.038 kb on + strand at 675.038 kb on + strand at 675.135 kb on - strand at 675.212 kb on + strand at 675.212 kb on + strand at 675.213 kb on - strand at 675.213 kb on - strand at 675.776 kb on - strand, within AZOBR_RS29190 at 675.776 kb on - strand, within AZOBR_RS29190 at 676.069 kb on + strand at 676.070 kb on - strand at 676.103 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 672,800 + AZOBR_RS29180 0.31 +1.9 672,801 - AZOBR_RS29180 0.31 -0.3 672,836 + AZOBR_RS29180 0.34 +1.9 672,836 + AZOBR_RS29180 0.34 +0.8 672,836 + AZOBR_RS29180 0.34 +1.0 672,854 + AZOBR_RS29180 0.35 -1.8 672,974 + AZOBR_RS29180 0.45 -0.3 672,974 + AZOBR_RS29180 0.45 -2.5 672,975 - AZOBR_RS29180 0.45 +0.1 673,532 + AZOBR_RS29180 0.90 +0.6 673,823 + +0.6 673,824 - +0.3 674,051 + AZOBR_RS29185 0.23 +0.4 674,204 + AZOBR_RS29185 0.33 -1.9 674,205 - AZOBR_RS29185 0.33 -0.5 674,789 + AZOBR_RS29185 0.74 -0.2 674,789 + AZOBR_RS29185 0.74 +0.7 674,790 - AZOBR_RS29185 0.74 -1.0 675,038 + -0.2 675,038 + +0.1 675,038 + +0.8 675,135 - -1.5 675,212 + -1.5 675,212 + +0.6 675,213 - -1.1 675,213 - +1.5 675,776 - AZOBR_RS29190 0.59 -1.0 675,776 - AZOBR_RS29190 0.59 -2.2 676,069 + +0.5 676,070 - -1.0 676,103 - -0.6
Or see this region's nucleotide sequence