Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS28165 and AZOBR_RS28170 are separated by 36 nucleotides AZOBR_RS28170 and AZOBR_RS28175 are separated by 126 nucleotides AZOBR_RS28175 and AZOBR_RS28180 are separated by 26 nucleotides
AZOBR_RS28165: AZOBR_RS28165 - hypothetical protein, at 490,501 to 490,743
_RS28165
AZOBR_RS28170: AZOBR_RS28170 - TetR family transcriptional regulator, at 490,780 to 491,376
_RS28170
AZOBR_RS28175: AZOBR_RS28175 - short-chain dehydrogenase, at 491,503 to 492,258
_RS28175
AZOBR_RS28180: AZOBR_RS28180 - acetyl-CoA acetyltransferase, at 492,285 to 493,478
_RS28180
Position (kb) 491
492
493 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 491.235 kb on - strand, within AZOBR_RS28170 at 491.512 kb on - strand at 491.966 kb on - strand, within AZOBR_RS28175 at 491.966 kb on - strand, within AZOBR_RS28175 at 492.273 kb on + strand at 492.274 kb on - strand at 492.663 kb on + strand, within AZOBR_RS28180 at 492.750 kb on + strand, within AZOBR_RS28180 at 492.750 kb on + strand, within AZOBR_RS28180 at 492.832 kb on - strand, within AZOBR_RS28180 at 492.832 kb on - strand, within AZOBR_RS28180
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 491,235 - AZOBR_RS28170 0.76 -2.0 491,512 - +0.8 491,966 - AZOBR_RS28175 0.61 +0.1 491,966 - AZOBR_RS28175 0.61 +0.3 492,273 + +0.3 492,274 - +0.3 492,663 + AZOBR_RS28180 0.32 -2.1 492,750 + AZOBR_RS28180 0.39 -2.3 492,750 + AZOBR_RS28180 0.39 -3.2 492,832 - AZOBR_RS28180 0.46 +0.5 492,832 - AZOBR_RS28180 0.46 -1.4
Or see this region's nucleotide sequence