Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS23695 and AZOBR_RS23700 are separated by 47 nucleotides AZOBR_RS23700 and AZOBR_RS23705 are separated by 203 nucleotides AZOBR_RS23705 and AZOBR_RS23710 are separated by 16 nucleotides AZOBR_RS23710 and AZOBR_RS23715 are separated by 218 nucleotides
AZOBR_RS23695: AZOBR_RS23695 - L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (from data) , at 349,974 to 353,681
_RS23695
AZOBR_RS23700: AZOBR_RS23700 - hypothetical protein, at 353,729 to 353,917
_RS23700
AZOBR_RS23705: AZOBR_RS23705 - beta-lactamase, at 354,121 to 355,170
_RS23705
AZOBR_RS23710: AZOBR_RS23710 - extensin, at 355,187 to 355,894
_RS23710
AZOBR_RS23715: AZOBR_RS23715 - hypothetical protein, at 356,113 to 356,418
_RS23715
Position (kb)
354
355
356 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 353.134 kb on - strand, within AZOBR_RS23695 at 353.190 kb on + strand, within AZOBR_RS23695 at 353.256 kb on + strand, within AZOBR_RS23695 at 353.256 kb on + strand at 353.730 kb on + strand at 354.238 kb on + strand, within AZOBR_RS23705 at 354.262 kb on + strand, within AZOBR_RS23705 at 354.263 kb on - strand, within AZOBR_RS23705 at 354.263 kb on - strand, within AZOBR_RS23705 at 354.263 kb on - strand, within AZOBR_RS23705 at 354.263 kb on - strand, within AZOBR_RS23705 at 354.320 kb on + strand, within AZOBR_RS23705 at 354.320 kb on + strand, within AZOBR_RS23705 at 354.320 kb on + strand, within AZOBR_RS23705 at 354.320 kb on + strand, within AZOBR_RS23705 at 354.320 kb on + strand, within AZOBR_RS23705 at 354.320 kb on + strand, within AZOBR_RS23705 at 354.321 kb on - strand, within AZOBR_RS23705 at 354.321 kb on - strand, within AZOBR_RS23705 at 354.321 kb on - strand, within AZOBR_RS23705 at 354.475 kb on + strand, within AZOBR_RS23705 at 354.541 kb on + strand, within AZOBR_RS23705 at 354.541 kb on + strand, within AZOBR_RS23705 at 354.542 kb on - strand, within AZOBR_RS23705 at 354.542 kb on - strand, within AZOBR_RS23705 at 354.602 kb on - strand, within AZOBR_RS23705 at 354.730 kb on + strand, within AZOBR_RS23705 at 354.731 kb on - strand, within AZOBR_RS23705 at 354.731 kb on - strand, within AZOBR_RS23705 at 354.838 kb on + strand, within AZOBR_RS23705 at 354.838 kb on + strand, within AZOBR_RS23705 at 354.838 kb on + strand, within AZOBR_RS23705 at 354.838 kb on + strand, within AZOBR_RS23705 at 354.839 kb on - strand, within AZOBR_RS23705 at 354.839 kb on - strand, within AZOBR_RS23705 at 354.839 kb on - strand, within AZOBR_RS23705 at 354.916 kb on + strand, within AZOBR_RS23705 at 354.917 kb on - strand, within AZOBR_RS23705 at 355.156 kb on + strand at 355.156 kb on + strand at 355.248 kb on + strand at 355.462 kb on - strand, within AZOBR_RS23710 at 355.462 kb on - strand, within AZOBR_RS23710 at 355.895 kb on - strand at 355.895 kb on - strand at 355.986 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 353,134 - AZOBR_RS23695 0.85 -2.5 353,190 + AZOBR_RS23695 0.87 -1.4 353,256 + AZOBR_RS23695 0.89 -1.5 353,256 + -1.2 353,730 + -0.2 354,238 + AZOBR_RS23705 0.11 -1.7 354,262 + AZOBR_RS23705 0.13 +1.0 354,263 - AZOBR_RS23705 0.14 -1.7 354,263 - AZOBR_RS23705 0.14 +0.5 354,263 - AZOBR_RS23705 0.14 +1.0 354,263 - AZOBR_RS23705 0.14 -1.2 354,320 + AZOBR_RS23705 0.19 +1.2 354,320 + AZOBR_RS23705 0.19 -0.3 354,320 + AZOBR_RS23705 0.19 -1.4 354,320 + AZOBR_RS23705 0.19 -0.1 354,320 + AZOBR_RS23705 0.19 +1.8 354,320 + AZOBR_RS23705 0.19 +0.3 354,321 - AZOBR_RS23705 0.19 +1.4 354,321 - AZOBR_RS23705 0.19 -0.8 354,321 - AZOBR_RS23705 0.19 -2.8 354,475 + AZOBR_RS23705 0.34 -4.6 354,541 + AZOBR_RS23705 0.40 +0.0 354,541 + AZOBR_RS23705 0.40 +0.2 354,542 - AZOBR_RS23705 0.40 +1.6 354,542 - AZOBR_RS23705 0.40 +0.5 354,602 - AZOBR_RS23705 0.46 -1.0 354,730 + AZOBR_RS23705 0.58 +1.4 354,731 - AZOBR_RS23705 0.58 -0.6 354,731 - AZOBR_RS23705 0.58 -3.6 354,838 + AZOBR_RS23705 0.68 +0.5 354,838 + AZOBR_RS23705 0.68 +0.6 354,838 + AZOBR_RS23705 0.68 -3.8 354,838 + AZOBR_RS23705 0.68 -4.2 354,839 - AZOBR_RS23705 0.68 +1.0 354,839 - AZOBR_RS23705 0.68 +0.6 354,839 - AZOBR_RS23705 0.68 +3.0 354,916 + AZOBR_RS23705 0.76 -3.9 354,917 - AZOBR_RS23705 0.76 -2.5 355,156 + +0.1 355,156 + +0.6 355,248 + -2.3 355,462 - AZOBR_RS23710 0.39 -0.0 355,462 - AZOBR_RS23710 0.39 -1.9 355,895 - -0.3 355,895 - +1.2 355,986 + +0.7
Or see this region's nucleotide sequence