Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS22505 and AZOBR_RS22510 are separated by 176 nucleotides AZOBR_RS22510 and AZOBR_RS22515 are separated by 79 nucleotides AZOBR_RS22515 and AZOBR_RS22520 are separated by 196 nucleotides
AZOBR_RS22505: AZOBR_RS22505 - omega amino acid--pyruvate aminotransferase, at 60,240 to 61,550
_RS22505
AZOBR_RS22510: AZOBR_RS22510 - LysR family transcriptional regulator, at 61,727 to 62,689
_RS22510
AZOBR_RS22515: AZOBR_RS22515 - membrane protein of unknown function, at 62,769 to 63,329
_RS22515
AZOBR_RS22520: AZOBR_RS22520 - hypothetical protein, at 63,526 to 63,918
_RS22520
Position (kb) 61
62
63 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 61.183 kb on + strand, within AZOBR_RS22505 at 61.330 kb on + strand, within AZOBR_RS22505 at 61.330 kb on + strand, within AZOBR_RS22505 at 61.331 kb on - strand, within AZOBR_RS22505 at 61.331 kb on - strand, within AZOBR_RS22505 at 61.331 kb on - strand, within AZOBR_RS22505 at 61.424 kb on - strand at 61.703 kb on - strand at 61.718 kb on + strand at 61.719 kb on - strand at 61.719 kb on - strand at 61.722 kb on + strand at 61.723 kb on - strand at 61.723 kb on - strand at 62.276 kb on + strand, within AZOBR_RS22510 at 62.277 kb on - strand, within AZOBR_RS22510 at 62.277 kb on - strand, within AZOBR_RS22510 at 62.465 kb on + strand, within AZOBR_RS22510 at 62.466 kb on - strand, within AZOBR_RS22510 at 62.490 kb on - strand, within AZOBR_RS22510 at 62.549 kb on + strand, within AZOBR_RS22510 at 62.802 kb on + strand at 62.802 kb on + strand at 62.802 kb on + strand at 63.377 kb on + strand at 63.378 kb on - strand at 63.525 kb on + strand at 63.525 kb on + strand at 63.526 kb on - strand at 63.535 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 61,183 + AZOBR_RS22505 0.72 +0.3 61,330 + AZOBR_RS22505 0.83 +0.5 61,330 + AZOBR_RS22505 0.83 +0.3 61,331 - AZOBR_RS22505 0.83 +0.1 61,331 - AZOBR_RS22505 0.83 -0.3 61,331 - AZOBR_RS22505 0.83 +0.3 61,424 - -0.1 61,703 - -2.1 61,718 + -0.0 61,719 - -0.1 61,719 - -3.9 61,722 + -1.2 61,723 - -0.8 61,723 - -0.1 62,276 + AZOBR_RS22510 0.57 -0.4 62,277 - AZOBR_RS22510 0.57 -0.2 62,277 - AZOBR_RS22510 0.57 -0.5 62,465 + AZOBR_RS22510 0.77 +0.8 62,466 - AZOBR_RS22510 0.77 +0.4 62,490 - AZOBR_RS22510 0.79 +0.0 62,549 + AZOBR_RS22510 0.85 +0.0 62,802 + +0.1 62,802 + -1.2 62,802 + -0.1 63,377 + +1.0 63,378 - +2.9 63,525 + -3.6 63,525 + +0.8 63,526 - -2.3 63,535 - +0.5
Or see this region's nucleotide sequence