Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS17700 and AZOBR_RS17705 are separated by 848 nucleotides AZOBR_RS17705 and AZOBR_RS17710 are separated by 129 nucleotides AZOBR_RS17710 and AZOBR_RS17715 are separated by 297 nucleotides
AZOBR_RS17700: AZOBR_RS17700 - putative N-acetylmuramoyl-L-alanine amidase, at 731,882 to 734,587
_RS17700
AZOBR_RS17705: AZOBR_RS17705 - hypothetical protein, at 735,436 to 736,497
_RS17705
AZOBR_RS17710: AZOBR_RS17710 - hypothetical protein, at 736,627 to 736,974
_RS17710
AZOBR_RS17715: AZOBR_RS17715 - glycosyl transferase, at 737,272 to 738,327
_RS17715
Position (kb)
735
736
737 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 734.948 kb on + strand at 734.948 kb on + strand at 734.949 kb on - strand at 734.949 kb on - strand at 735.094 kb on + strand at 735.095 kb on - strand at 735.258 kb on + strand at 735.259 kb on - strand at 736.022 kb on - strand, within AZOBR_RS17705 at 736.329 kb on - strand, within AZOBR_RS17705 at 736.786 kb on - strand, within AZOBR_RS17710 at 736.792 kb on + strand, within AZOBR_RS17710 at 736.817 kb on - strand, within AZOBR_RS17710 at 736.817 kb on - strand, within AZOBR_RS17710 at 736.842 kb on + strand, within AZOBR_RS17710 at 736.980 kb on + strand at 736.980 kb on + strand at 736.980 kb on + strand at 736.981 kb on - strand at 736.981 kb on - strand at 736.981 kb on - strand at 737.168 kb on + strand at 737.168 kb on + strand at 737.169 kb on - strand at 737.177 kb on - strand at 737.300 kb on - strand at 737.300 kb on - strand at 737.350 kb on + strand at 737.351 kb on - strand at 737.351 kb on - strand at 737.351 kb on - strand at 737.351 kb on - strand at 737.351 kb on - strand at 737.418 kb on + strand, within AZOBR_RS17715 at 737.419 kb on - strand, within AZOBR_RS17715 at 737.419 kb on - strand, within AZOBR_RS17715
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 734,948 + +2.1 734,948 + -1.6 734,949 - +2.1 734,949 - -2.6 735,094 + -1.4 735,095 - +0.1 735,258 + +1.4 735,259 - +0.9 736,022 - AZOBR_RS17705 0.55 +1.5 736,329 - AZOBR_RS17705 0.84 +1.3 736,786 - AZOBR_RS17710 0.46 -2.4 736,792 + AZOBR_RS17710 0.47 -2.5 736,817 - AZOBR_RS17710 0.55 +0.9 736,817 - AZOBR_RS17710 0.55 -0.8 736,842 + AZOBR_RS17710 0.62 +0.8 736,980 + -4.9 736,980 + -1.3 736,980 + +2.4 736,981 - -1.5 736,981 - +0.1 736,981 - +0.3 737,168 + -0.9 737,168 + -1.1 737,169 - +0.9 737,177 - -0.8 737,300 - -0.7 737,300 - -0.4 737,350 + -4.1 737,351 - +0.7 737,351 - -0.1 737,351 - +0.8 737,351 - +0.5 737,351 - +0.3 737,418 + AZOBR_RS17715 0.14 -1.9 737,419 - AZOBR_RS17715 0.14 -0.6 737,419 - AZOBR_RS17715 0.14 -1.2
Or see this region's nucleotide sequence