Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS17000 and AZOBR_RS17005 are separated by 298 nucleotides AZOBR_RS17005 and AZOBR_RS17010 overlap by 60 nucleotides AZOBR_RS17010 and AZOBR_RS17015 are separated by 169 nucleotides AZOBR_RS17015 and AZOBR_RS17020 are separated by 9 nucleotides
AZOBR_RS17000: AZOBR_RS17000 - 1-deoxy-D-xylulose-5-phosphate synthase, at 568,540 to 570,471
_RS17000
AZOBR_RS17005: AZOBR_RS17005 - radical SAM protein, at 570,770 to 571,897
_RS17005
AZOBR_RS17010: AZOBR_RS17010 - hypothetical protein, at 571,838 to 572,107
_RS17010
AZOBR_RS17015: AZOBR_RS17015 - hypothetical protein, at 572,277 to 572,663
_RS17015
AZOBR_RS17020: AZOBR_RS17020 - hypothetical protein, at 572,673 to 573,068
_RS17020
Position (kb)
570
571
572 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 569.883 kb on - strand, within AZOBR_RS17000 at 569.985 kb on - strand, within AZOBR_RS17000 at 570.122 kb on + strand, within AZOBR_RS17000 at 570.122 kb on + strand, within AZOBR_RS17000 at 570.135 kb on + strand, within AZOBR_RS17000 at 570.136 kb on - strand, within AZOBR_RS17000 at 570.136 kb on - strand, within AZOBR_RS17000 at 570.136 kb on - strand, within AZOBR_RS17000 at 570.219 kb on - strand, within AZOBR_RS17000 at 570.272 kb on + strand, within AZOBR_RS17000 at 570.272 kb on + strand, within AZOBR_RS17000 at 570.272 kb on + strand, within AZOBR_RS17000 at 570.272 kb on + strand, within AZOBR_RS17000 at 570.272 kb on + strand, within AZOBR_RS17000 at 570.272 kb on + strand, within AZOBR_RS17000 at 570.272 kb on + strand, within AZOBR_RS17000 at 570.273 kb on - strand, within AZOBR_RS17000 at 570.273 kb on - strand, within AZOBR_RS17000 at 570.273 kb on - strand, within AZOBR_RS17000 at 570.491 kb on - strand at 570.502 kb on + strand at 570.503 kb on - strand at 570.503 kb on - strand at 570.503 kb on - strand at 570.610 kb on + strand at 570.611 kb on - strand at 570.611 kb on - strand at 570.615 kb on + strand at 570.615 kb on + strand at 570.616 kb on - strand at 570.620 kb on + strand at 570.620 kb on + strand at 570.621 kb on - strand at 570.621 kb on - strand at 570.621 kb on - strand at 570.621 kb on - strand at 570.625 kb on + strand at 570.625 kb on + strand at 570.625 kb on + strand at 570.626 kb on - strand at 570.809 kb on + strand at 570.809 kb on + strand at 570.810 kb on - strand at 570.810 kb on - strand at 570.917 kb on + strand, within AZOBR_RS17005 at 571.079 kb on + strand, within AZOBR_RS17005 at 571.079 kb on + strand, within AZOBR_RS17005 at 571.211 kb on + strand, within AZOBR_RS17005 at 571.211 kb on + strand, within AZOBR_RS17005 at 571.370 kb on + strand, within AZOBR_RS17005 at 571.376 kb on + strand, within AZOBR_RS17005 at 571.523 kb on + strand, within AZOBR_RS17005 at 571.523 kb on + strand, within AZOBR_RS17005 at 571.524 kb on - strand, within AZOBR_RS17005 at 571.583 kb on + strand, within AZOBR_RS17005 at 571.584 kb on - strand, within AZOBR_RS17005 at 571.584 kb on - strand, within AZOBR_RS17005 at 571.649 kb on + strand, within AZOBR_RS17005 at 571.650 kb on - strand, within AZOBR_RS17005 at 571.664 kb on + strand, within AZOBR_RS17005 at 571.665 kb on - strand, within AZOBR_RS17005 at 571.665 kb on - strand, within AZOBR_RS17005 at 572.432 kb on - strand, within AZOBR_RS17015 at 572.857 kb on + strand, within AZOBR_RS17020 at 572.858 kb on - strand, within AZOBR_RS17020 at 572.873 kb on - strand, within AZOBR_RS17020 at 572.873 kb on - strand, within AZOBR_RS17020 at 572.873 kb on - strand, within AZOBR_RS17020
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 569,883 - AZOBR_RS17000 0.70 +0.9 569,985 - AZOBR_RS17000 0.75 -3.3 570,122 + AZOBR_RS17000 0.82 -1.7 570,122 + AZOBR_RS17000 0.82 +0.2 570,135 + AZOBR_RS17000 0.83 -0.3 570,136 - AZOBR_RS17000 0.83 -2.4 570,136 - AZOBR_RS17000 0.83 -3.4 570,136 - AZOBR_RS17000 0.83 -4.5 570,219 - AZOBR_RS17000 0.87 +3.3 570,272 + AZOBR_RS17000 0.90 -2.6 570,272 + AZOBR_RS17000 0.90 +2.1 570,272 + AZOBR_RS17000 0.90 +0.3 570,272 + AZOBR_RS17000 0.90 -0.9 570,272 + AZOBR_RS17000 0.90 +0.1 570,272 + AZOBR_RS17000 0.90 +1.2 570,272 + AZOBR_RS17000 0.90 -1.4 570,273 - AZOBR_RS17000 0.90 -0.6 570,273 - AZOBR_RS17000 0.90 -0.3 570,273 - AZOBR_RS17000 0.90 -1.3 570,491 - +0.0 570,502 + +0.7 570,503 - +1.7 570,503 - +0.5 570,503 - +0.6 570,610 + +0.7 570,611 - -1.3 570,611 - -0.2 570,615 + -1.6 570,615 + -0.1 570,616 - +0.3 570,620 + -0.1 570,620 + -2.1 570,621 - -0.5 570,621 - +1.2 570,621 - +0.1 570,621 - +1.1 570,625 + -1.8 570,625 + +1.3 570,625 + -1.6 570,626 - +0.2 570,809 + +0.4 570,809 + -0.3 570,810 - +0.7 570,810 - -0.2 570,917 + AZOBR_RS17005 0.13 -0.8 571,079 + AZOBR_RS17005 0.27 +0.5 571,079 + AZOBR_RS17005 0.27 -0.1 571,211 + AZOBR_RS17005 0.39 +1.4 571,211 + AZOBR_RS17005 0.39 -0.7 571,370 + AZOBR_RS17005 0.53 -0.3 571,376 + AZOBR_RS17005 0.54 -0.6 571,523 + AZOBR_RS17005 0.67 +1.3 571,523 + AZOBR_RS17005 0.67 +0.0 571,524 - AZOBR_RS17005 0.67 +0.9 571,583 + AZOBR_RS17005 0.72 +0.3 571,584 - AZOBR_RS17005 0.72 +0.9 571,584 - AZOBR_RS17005 0.72 -0.0 571,649 + AZOBR_RS17005 0.78 +0.4 571,650 - AZOBR_RS17005 0.78 +0.1 571,664 + AZOBR_RS17005 0.79 -0.1 571,665 - AZOBR_RS17005 0.79 +0.7 571,665 - AZOBR_RS17005 0.79 -0.8 572,432 - AZOBR_RS17015 0.40 -3.3 572,857 + AZOBR_RS17020 0.46 +1.0 572,858 - AZOBR_RS17020 0.47 +0.5 572,873 - AZOBR_RS17020 0.51 +1.0 572,873 - AZOBR_RS17020 0.51 +1.0 572,873 - AZOBR_RS17020 0.51 -0.1
Or see this region's nucleotide sequence