Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS03210 and AZOBR_RS03215 are separated by 136 nucleotides AZOBR_RS03215 and AZOBR_RS03220 are separated by 113 nucleotides AZOBR_RS03220 and AZOBR_RS03225 are separated by 91 nucleotides
AZOBR_RS03210: AZOBR_RS03210 - hypothetical protein, at 682,141 to 682,755
_RS03210
AZOBR_RS03215: AZOBR_RS03215 - hypothetical protein, at 682,892 to 683,377
_RS03215
AZOBR_RS03220: AZOBR_RS03220 - 3-dehydroquinate synthase, at 683,491 to 684,627
_RS03220
AZOBR_RS03225: AZOBR_RS03225 - shikimate kinase, at 684,719 to 685,207
_RS03225
Position (kb) 683
684
685 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 682.634 kb on + strand, within AZOBR_RS03210 at 682.634 kb on + strand, within AZOBR_RS03210 at 682.634 kb on + strand, within AZOBR_RS03210 at 682.634 kb on + strand, within AZOBR_RS03210 at 682.635 kb on - strand, within AZOBR_RS03210 at 682.635 kb on - strand, within AZOBR_RS03210 at 682.778 kb on - strand at 683.091 kb on + strand, within AZOBR_RS03215 at 683.477 kb on + strand at 683.478 kb on - strand at 685.389 kb on - strand at 685.389 kb on - strand at 685.389 kb on - strand at 685.442 kb on + strand at 685.492 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 682,634 + AZOBR_RS03210 0.80 -0.7 682,634 + AZOBR_RS03210 0.80 +0.8 682,634 + AZOBR_RS03210 0.80 -0.1 682,634 + AZOBR_RS03210 0.80 -0.0 682,635 - AZOBR_RS03210 0.80 -1.2 682,635 - AZOBR_RS03210 0.80 +0.5 682,778 - -1.0 683,091 + AZOBR_RS03215 0.41 -2.0 683,477 + -0.0 683,478 - -1.8 685,389 - -3.4 685,389 - -2.3 685,389 - +0.6 685,442 + -1.2 685,492 - -2.2
Or see this region's nucleotide sequence