Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS03130 and AZOBR_RS03135 are separated by 260 nucleotides AZOBR_RS03135 and AZOBR_RS03140 are separated by 12 nucleotides AZOBR_RS03140 and AZOBR_RS03145 are separated by 184 nucleotides
AZOBR_RS03130: AZOBR_RS03130 - hypothetical protein, at 667,704 to 668,327
_RS03130
AZOBR_RS03135: AZOBR_RS03135 - orotidine 5'-phosphate decarboxylase, at 668,588 to 669,301
_RS03135
AZOBR_RS03140: AZOBR_RS03140 - N-(5'-phosphoribosyl)anthranilate isomerase, at 669,314 to 669,958
_RS03140
AZOBR_RS03145: AZOBR_RS03145 - diguanylate cyclase, at 670,143 to 671,018
_RS03145
Position (kb) 669
670 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 668.539 kb on + strand at 668.868 kb on + strand, within AZOBR_RS03135 at 668.869 kb on - strand at 668.869 kb on - strand, within AZOBR_RS03135 at 668.869 kb on - strand at 669.299 kb on + strand at 669.299 kb on + strand at 669.299 kb on + strand at 669.299 kb on + strand at 669.300 kb on - strand at 669.313 kb on + strand at 669.390 kb on - strand at 670.036 kb on + strand at 670.037 kb on - strand at 670.437 kb on + strand, within AZOBR_RS03145 at 670.437 kb on + strand, within AZOBR_RS03145 at 670.662 kb on + strand, within AZOBR_RS03145 at 670.662 kb on + strand, within AZOBR_RS03145
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 668,539 + +2.0 668,868 + AZOBR_RS03135 0.39 +1.5 668,869 - +0.9 668,869 - AZOBR_RS03135 0.39 -2.8 668,869 - +0.9 669,299 + +3.6 669,299 + -0.4 669,299 + +0.4 669,299 + +1.7 669,300 - +0.1 669,313 + +1.0 669,390 - -0.5 670,036 + +0.0 670,037 - +0.6 670,437 + AZOBR_RS03145 0.34 +0.3 670,437 + AZOBR_RS03145 0.34 +1.6 670,662 + AZOBR_RS03145 0.59 -2.9 670,662 + AZOBR_RS03145 0.59 -1.7
Or see this region's nucleotide sequence