Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01760 and AZOBR_RS01765 overlap by 4 nucleotides AZOBR_RS01765 and AZOBR_RS01770 are separated by 9 nucleotides AZOBR_RS01770 and AZOBR_RS01775 overlap by 8 nucleotides AZOBR_RS01775 and AZOBR_RS01780 are separated by 41 nucleotides
AZOBR_RS01760: AZOBR_RS01760 - septum formation protein Maf, at 359,034 to 359,642
_RS01760
AZOBR_RS01765: AZOBR_RS01765 - shikimate 5-dehydrogenase, at 359,639 to 360,478
_RS01765
AZOBR_RS01770: AZOBR_RS01770 - dephospho-CoA kinase, at 360,488 to 361,132
_RS01770
AZOBR_RS01775: AZOBR_RS01775 - DNA polymerase III subunit epsilon, at 361,125 to 361,802
_RS01775
AZOBR_RS01780: AZOBR_RS01780 - quinone oxidoreductase, at 361,844 to 362,818
_RS01780
Position (kb) 360
361
362 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 359.517 kb on + strand, within AZOBR_RS01760 at 359.517 kb on + strand, within AZOBR_RS01760 at 361.103 kb on + strand at 361.103 kb on + strand at 361.248 kb on + strand, within AZOBR_RS01775 at 361.249 kb on - strand, within AZOBR_RS01775 at 361.569 kb on + strand, within AZOBR_RS01775 at 361.612 kb on - strand, within AZOBR_RS01775 at 361.612 kb on - strand, within AZOBR_RS01775 at 361.612 kb on - strand, within AZOBR_RS01775 at 361.612 kb on - strand, within AZOBR_RS01775 at 361.957 kb on + strand, within AZOBR_RS01780 at 361.957 kb on + strand, within AZOBR_RS01780 at 361.958 kb on - strand, within AZOBR_RS01780 at 362.016 kb on + strand, within AZOBR_RS01780 at 362.016 kb on + strand, within AZOBR_RS01780 at 362.017 kb on - strand, within AZOBR_RS01780 at 362.017 kb on - strand, within AZOBR_RS01780 at 362.043 kb on + strand, within AZOBR_RS01780 at 362.043 kb on + strand, within AZOBR_RS01780 at 362.044 kb on - strand, within AZOBR_RS01780 at 362.044 kb on - strand, within AZOBR_RS01780 at 362.044 kb on - strand, within AZOBR_RS01780 at 362.044 kb on - strand, within AZOBR_RS01780 at 362.119 kb on + strand, within AZOBR_RS01780 at 362.119 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 359,517 + AZOBR_RS01760 0.79 +0.2 359,517 + AZOBR_RS01760 0.79 +2.5 361,103 + +0.1 361,103 + +0.3 361,248 + AZOBR_RS01775 0.18 -1.9 361,249 - AZOBR_RS01775 0.18 -3.1 361,569 + AZOBR_RS01775 0.65 -1.9 361,612 - AZOBR_RS01775 0.72 -2.6 361,612 - AZOBR_RS01775 0.72 -2.7 361,612 - AZOBR_RS01775 0.72 -3.1 361,612 - AZOBR_RS01775 0.72 -4.0 361,957 + AZOBR_RS01780 0.12 +0.3 361,957 + AZOBR_RS01780 0.12 -1.0 361,958 - AZOBR_RS01780 0.12 -4.2 362,016 + AZOBR_RS01780 0.18 +0.6 362,016 + AZOBR_RS01780 0.18 -0.8 362,017 - AZOBR_RS01780 0.18 -1.1 362,017 - AZOBR_RS01780 0.18 -0.3 362,043 + AZOBR_RS01780 0.20 -0.6 362,043 + AZOBR_RS01780 0.20 -0.2 362,044 - AZOBR_RS01780 0.21 +1.0 362,044 - AZOBR_RS01780 0.21 -2.9 362,044 - AZOBR_RS01780 0.21 -0.2 362,044 - AZOBR_RS01780 0.21 +1.1 362,119 + AZOBR_RS01780 0.28 -3.1 362,119 + -0.2
Or see this region's nucleotide sequence