Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01070 and AZOBR_RS01075 overlap by 1 nucleotides AZOBR_RS01075 and AZOBR_RS01080 are separated by 75 nucleotides AZOBR_RS01080 and AZOBR_RS01085 are separated by 309 nucleotides
AZOBR_RS01070: AZOBR_RS01070 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 218,753 to 219,247
_RS01070
AZOBR_RS01075: AZOBR_RS01075 - Rrf2 family transcriptional regulator, at 219,247 to 219,702
_RS01075
AZOBR_RS01080: AZOBR_RS01080 - cysteine synthase, at 219,778 to 220,737
_RS01080
AZOBR_RS01085: AZOBR_RS01085 - hypothetical protein, at 221,047 to 221,820
_RS01085
Position (kb) 219
220
221 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 219.693 kb on + strand at 220.702 kb on + strand at 220.702 kb on + strand at 220.702 kb on + strand at 220.703 kb on - strand at 220.736 kb on - strand at 220.790 kb on + strand at 220.791 kb on - strand at 220.819 kb on + strand at 220.844 kb on + strand at 220.844 kb on + strand at 220.845 kb on - strand at 220.924 kb on + strand at 221.141 kb on - strand, within AZOBR_RS01085 at 221.299 kb on + strand, within AZOBR_RS01085 at 221.300 kb on - strand, within AZOBR_RS01085 at 221.300 kb on - strand, within AZOBR_RS01085 at 221.300 kb on - strand, within AZOBR_RS01085 at 221.301 kb on + strand, within AZOBR_RS01085 at 221.302 kb on - strand, within AZOBR_RS01085 at 221.302 kb on - strand, within AZOBR_RS01085 at 221.348 kb on - strand, within AZOBR_RS01085 at 221.680 kb on + strand, within AZOBR_RS01085
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 219,693 + -1.1 220,702 + +0.2 220,702 + -3.2 220,702 + -0.4 220,703 - -2.5 220,736 - +0.3 220,790 + +1.6 220,791 - +1.1 220,819 + -1.8 220,844 + -1.1 220,844 + +0.8 220,845 - +1.3 220,924 + -0.2 221,141 - AZOBR_RS01085 0.12 -0.3 221,299 + AZOBR_RS01085 0.33 +0.2 221,300 - AZOBR_RS01085 0.33 +0.7 221,300 - AZOBR_RS01085 0.33 -2.9 221,300 - AZOBR_RS01085 0.33 +1.7 221,301 + AZOBR_RS01085 0.33 -0.7 221,302 - AZOBR_RS01085 0.33 -1.1 221,302 - AZOBR_RS01085 0.33 +0.5 221,348 - AZOBR_RS01085 0.39 -0.6 221,680 + AZOBR_RS01085 0.82 +0.2
Or see this region's nucleotide sequence