Experiment: D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS00630 and AZOBR_RS00635 are separated by 134 nucleotides AZOBR_RS00635 and AZOBR_RS00640 are separated by 155 nucleotides AZOBR_RS00640 and AZOBR_RS00645 are separated by 20 nucleotides
AZOBR_RS00630: AZOBR_RS00630 - acetylglutamate kinase, at 127,733 to 128,146
_RS00630
AZOBR_RS00635: AZOBR_RS00635 - pterin-4-alpha-carbinolamine dehydratase, at 128,281 to 128,583
_RS00635
AZOBR_RS00640: AZOBR_RS00640 - pyrophosphorylase, at 128,739 to 129,566
_RS00640
AZOBR_RS00645: AZOBR_RS00645 - magnesium transporter, at 129,587 to 130,576
_RS00645
Position (kb) 128
129
130 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 127.854 kb on + strand, within AZOBR_RS00630 at 127.930 kb on + strand, within AZOBR_RS00630 at 127.930 kb on + strand, within AZOBR_RS00630 at 128.146 kb on + strand at 128.147 kb on - strand at 128.158 kb on + strand at 128.159 kb on - strand at 128.159 kb on - strand at 128.246 kb on - strand at 128.377 kb on + strand, within AZOBR_RS00635 at 128.648 kb on - strand at 128.648 kb on - strand at 128.650 kb on - strand at 128.828 kb on - strand, within AZOBR_RS00640 at 129.588 kb on + strand at 129.588 kb on + strand at 130.356 kb on + strand, within AZOBR_RS00645 at 130.356 kb on + strand, within AZOBR_RS00645 at 130.356 kb on + strand, within AZOBR_RS00645 at 130.357 kb on - strand, within AZOBR_RS00645 at 130.357 kb on - strand, within AZOBR_RS00645 at 130.378 kb on - strand, within AZOBR_RS00645
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml remove 127,854 + AZOBR_RS00630 0.29 -1.5 127,930 + AZOBR_RS00630 0.48 -0.6 127,930 + AZOBR_RS00630 0.48 -1.1 128,146 + +2.0 128,147 - +0.4 128,158 + -0.0 128,159 - +2.5 128,159 - -3.3 128,246 - +1.1 128,377 + AZOBR_RS00635 0.32 -4.0 128,648 - -2.5 128,648 - +0.5 128,650 - +0.0 128,828 - AZOBR_RS00640 0.11 +1.0 129,588 + -1.5 129,588 + -1.1 130,356 + AZOBR_RS00645 0.78 -1.3 130,356 + AZOBR_RS00645 0.78 +1.0 130,356 + AZOBR_RS00645 0.78 -2.0 130,357 - AZOBR_RS00645 0.78 -3.7 130,357 - AZOBR_RS00645 0.78 -0.7 130,378 - AZOBR_RS00645 0.80 -2.4
Or see this region's nucleotide sequence