Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22965 and EX31_RS22970 are separated by 172 nucleotides EX31_RS22970 and EX31_RS22975 are separated by 526 nucleotides
EX31_RS22965: EX31_RS22965 - DUF2461 domain-containing protein, at 356,034 to 356,747
_RS22965
EX31_RS22970: EX31_RS22970 - hypothetical protein, at 356,920 to 357,249
_RS22970
EX31_RS22975: EX31_RS22975 - sugar porter family MFS transporter, at 357,776 to 359,191
_RS22975
Position (kb) 356
357
358 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 355.959 kb on - strand at 355.959 kb on - strand at 355.961 kb on - strand at 356.026 kb on + strand at 356.065 kb on + strand at 356.065 kb on + strand at 356.310 kb on - strand, within EX31_RS22965 at 356.395 kb on + strand, within EX31_RS22965 at 356.396 kb on - strand, within EX31_RS22965 at 356.396 kb on - strand, within EX31_RS22965 at 356.396 kb on - strand, within EX31_RS22965 at 356.396 kb on - strand, within EX31_RS22965 at 356.412 kb on + strand, within EX31_RS22965 at 356.412 kb on + strand, within EX31_RS22965 at 356.412 kb on + strand, within EX31_RS22965 at 356.412 kb on + strand, within EX31_RS22965 at 356.413 kb on - strand, within EX31_RS22965 at 356.413 kb on - strand, within EX31_RS22965 at 356.460 kb on - strand, within EX31_RS22965 at 356.460 kb on - strand, within EX31_RS22965 at 356.561 kb on + strand, within EX31_RS22965 at 356.562 kb on - strand, within EX31_RS22965 at 356.621 kb on - strand, within EX31_RS22965 at 356.633 kb on + strand, within EX31_RS22965 at 356.640 kb on - strand, within EX31_RS22965 at 356.672 kb on + strand, within EX31_RS22965 at 356.730 kb on - strand at 356.730 kb on - strand at 356.828 kb on + strand at 356.829 kb on - strand at 356.914 kb on + strand at 356.914 kb on + strand at 356.915 kb on - strand at 357.004 kb on + strand, within EX31_RS22970 at 357.004 kb on + strand, within EX31_RS22970 at 357.004 kb on + strand, within EX31_RS22970 at 357.005 kb on - strand, within EX31_RS22970 at 357.005 kb on - strand, within EX31_RS22970 at 357.005 kb on - strand, within EX31_RS22970 at 357.027 kb on - strand, within EX31_RS22970 at 357.027 kb on - strand, within EX31_RS22970 at 357.061 kb on + strand, within EX31_RS22970 at 357.062 kb on - strand, within EX31_RS22970 at 357.062 kb on - strand, within EX31_RS22970 at 357.062 kb on - strand, within EX31_RS22970 at 357.062 kb on - strand, within EX31_RS22970 at 357.062 kb on - strand, within EX31_RS22970 at 357.062 kb on - strand, within EX31_RS22970 at 357.062 kb on - strand, within EX31_RS22970 at 357.149 kb on + strand, within EX31_RS22970 at 357.252 kb on + strand at 357.263 kb on - strand at 357.263 kb on - strand at 357.263 kb on - strand at 357.270 kb on + strand at 357.270 kb on + strand at 357.270 kb on + strand at 357.271 kb on - strand at 357.271 kb on - strand at 357.271 kb on - strand at 357.271 kb on - strand at 357.326 kb on + strand at 357.339 kb on - strand at 357.339 kb on - strand at 357.339 kb on - strand at 357.383 kb on + strand at 357.384 kb on - strand at 357.384 kb on - strand at 357.384 kb on - strand at 357.384 kb on - strand at 357.384 kb on - strand at 357.384 kb on - strand at 357.412 kb on + strand at 357.412 kb on + strand at 357.413 kb on - strand at 357.413 kb on - strand at 357.416 kb on - strand at 357.430 kb on - strand at 357.433 kb on + strand at 357.433 kb on + strand at 357.433 kb on + strand at 357.433 kb on + strand at 357.433 kb on + strand at 357.433 kb on + strand at 357.433 kb on + strand at 357.433 kb on + strand at 357.433 kb on + strand at 357.433 kb on + strand at 357.433 kb on + strand at 357.433 kb on + strand at 357.433 kb on + strand at 357.434 kb on - strand at 357.434 kb on - strand at 357.434 kb on - strand at 357.434 kb on - strand at 357.434 kb on - strand at 357.434 kb on - strand at 357.434 kb on - strand at 357.434 kb on - strand at 357.434 kb on - strand at 357.434 kb on - strand at 357.434 kb on - strand at 357.450 kb on + strand at 357.489 kb on - strand at 357.489 kb on - strand at 357.489 kb on - strand at 357.557 kb on + strand at 357.588 kb on + strand at 357.588 kb on + strand at 357.665 kb on + strand at 357.665 kb on + strand at 357.665 kb on + strand at 357.666 kb on - strand at 357.666 kb on - strand at 357.666 kb on - strand at 357.666 kb on - strand at 357.666 kb on - strand at 357.666 kb on - strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.667 kb on + strand at 357.668 kb on - strand at 357.668 kb on - strand at 357.668 kb on - strand at 357.668 kb on - strand at 357.668 kb on - strand at 357.668 kb on - strand at 357.668 kb on - strand at 357.668 kb on - strand at 357.668 kb on - strand at 357.668 kb on - strand at 357.668 kb on - strand at 357.668 kb on - strand at 357.668 kb on - strand at 357.668 kb on - strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.669 kb on + strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.670 kb on - strand at 357.711 kb on - strand at 357.752 kb on + strand at 357.752 kb on + strand at 357.753 kb on - strand at 357.796 kb on + strand at 357.919 kb on + strand, within EX31_RS22975 at 357.919 kb on + strand, within EX31_RS22975 at 357.922 kb on + strand, within EX31_RS22975 at 357.922 kb on + strand, within EX31_RS22975 at 357.922 kb on + strand, within EX31_RS22975 at 358.069 kb on + strand, within EX31_RS22975 at 358.069 kb on + strand, within EX31_RS22975 at 358.070 kb on - strand, within EX31_RS22975 at 358.070 kb on - strand, within EX31_RS22975 at 358.070 kb on - strand, within EX31_RS22975 at 358.179 kb on - strand, within EX31_RS22975 at 358.179 kb on - strand, within EX31_RS22975 at 358.229 kb on + strand, within EX31_RS22975 at 358.229 kb on + strand, within EX31_RS22975 at 358.230 kb on - strand, within EX31_RS22975 at 358.230 kb on - strand, within EX31_RS22975 at 358.230 kb on - strand, within EX31_RS22975
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 355,959 - -0.5 355,959 - -1.3 355,961 - +0.9 356,026 + -0.1 356,065 + -0.8 356,065 + -0.5 356,310 - EX31_RS22965 0.39 -1.6 356,395 + EX31_RS22965 0.51 -1.9 356,396 - EX31_RS22965 0.51 -0.6 356,396 - EX31_RS22965 0.51 +0.7 356,396 - EX31_RS22965 0.51 -1.3 356,396 - EX31_RS22965 0.51 -2.1 356,412 + EX31_RS22965 0.53 -1.0 356,412 + EX31_RS22965 0.53 +0.8 356,412 + EX31_RS22965 0.53 +1.2 356,412 + EX31_RS22965 0.53 -0.5 356,413 - EX31_RS22965 0.53 -1.5 356,413 - EX31_RS22965 0.53 +0.8 356,460 - EX31_RS22965 0.60 -0.5 356,460 - EX31_RS22965 0.60 -1.1 356,561 + EX31_RS22965 0.74 -0.1 356,562 - EX31_RS22965 0.74 +0.3 356,621 - EX31_RS22965 0.82 +1.0 356,633 + EX31_RS22965 0.84 -0.5 356,640 - EX31_RS22965 0.85 -0.5 356,672 + EX31_RS22965 0.89 +0.5 356,730 - +0.9 356,730 - -0.2 356,828 + -0.3 356,829 - -0.7 356,914 + +0.9 356,914 + +0.4 356,915 - -0.6 357,004 + EX31_RS22970 0.25 +0.8 357,004 + EX31_RS22970 0.25 -1.1 357,004 + EX31_RS22970 0.25 +0.4 357,005 - EX31_RS22970 0.26 +1.6 357,005 - EX31_RS22970 0.26 -0.5 357,005 - EX31_RS22970 0.26 -0.9 357,027 - EX31_RS22970 0.32 +0.8 357,027 - EX31_RS22970 0.32 +0.4 357,061 + EX31_RS22970 0.43 -0.1 357,062 - EX31_RS22970 0.43 -0.2 357,062 - EX31_RS22970 0.43 +1.2 357,062 - EX31_RS22970 0.43 -0.8 357,062 - EX31_RS22970 0.43 +0.3 357,062 - EX31_RS22970 0.43 -0.0 357,062 - EX31_RS22970 0.43 +0.6 357,062 - EX31_RS22970 0.43 +0.3 357,149 + EX31_RS22970 0.69 +0.4 357,252 + -1.0 357,263 - -0.5 357,263 - -0.3 357,263 - +1.2 357,270 + +0.6 357,270 + +1.5 357,270 + -0.9 357,271 - -0.7 357,271 - +0.1 357,271 - +0.6 357,271 - -0.1 357,326 + +0.5 357,339 - +0.7 357,339 - -1.1 357,339 - -1.2 357,383 + +0.1 357,384 - +0.6 357,384 - -0.3 357,384 - -0.5 357,384 - -0.1 357,384 - -1.1 357,384 - +0.8 357,412 + +1.1 357,412 + +0.7 357,413 - -0.4 357,413 - -0.3 357,416 - +0.7 357,430 - +0.5 357,433 + -0.8 357,433 + -1.4 357,433 + +0.6 357,433 + -1.0 357,433 + -0.2 357,433 + +0.2 357,433 + -0.4 357,433 + -0.9 357,433 + +0.7 357,433 + -0.4 357,433 + +0.3 357,433 + -1.7 357,433 + +0.7 357,434 - +0.8 357,434 - +0.2 357,434 - -0.0 357,434 - +0.3 357,434 - +0.1 357,434 - +0.4 357,434 - +1.8 357,434 - -0.5 357,434 - -1.1 357,434 - -0.4 357,434 - -0.2 357,450 + +0.5 357,489 - -0.5 357,489 - -0.0 357,489 - -1.3 357,557 + -0.3 357,588 + +2.0 357,588 + +0.3 357,665 + +1.5 357,665 + -0.2 357,665 + +0.0 357,666 - -0.5 357,666 - -0.9 357,666 - +0.8 357,666 - +0.1 357,666 - +0.6 357,666 - +0.4 357,667 + +0.2 357,667 + +0.2 357,667 + +0.5 357,667 + +1.0 357,667 + +1.8 357,667 + +0.6 357,667 + +0.8 357,667 + +0.8 357,667 + +1.1 357,667 + +0.1 357,667 + +1.1 357,667 + +0.1 357,667 + -3.1 357,667 + +0.2 357,667 + +0.1 357,667 + +0.5 357,668 - -0.3 357,668 - -1.7 357,668 - +1.4 357,668 - +0.9 357,668 - +0.8 357,668 - +0.2 357,668 - -3.1 357,668 - -0.2 357,668 - +1.2 357,668 - -0.8 357,668 - -0.0 357,668 - +0.2 357,668 - +0.8 357,668 - +0.3 357,669 + -0.8 357,669 + +0.3 357,669 + -0.2 357,669 + -0.5 357,669 + +0.8 357,669 + +0.2 357,669 + +0.1 357,669 + -0.2 357,669 + +0.1 357,669 + +0.9 357,669 + -1.4 357,669 + +0.7 357,669 + -0.2 357,669 + +0.8 357,669 + -1.0 357,669 + +0.2 357,669 + -1.9 357,669 + +0.0 357,669 + +1.0 357,670 - +0.2 357,670 - +0.6 357,670 - +0.9 357,670 - -1.0 357,670 - -0.5 357,670 - -1.8 357,670 - -1.4 357,670 - +0.8 357,670 - +1.3 357,670 - +0.4 357,670 - +0.2 357,670 - +0.2 357,670 - +0.1 357,670 - -1.2 357,670 - -0.2 357,670 - -0.2 357,670 - -1.8 357,670 - -1.9 357,670 - +0.2 357,670 - +0.8 357,670 - -0.2 357,670 - +0.8 357,670 - -0.8 357,670 - +1.0 357,670 - +0.6 357,670 - +0.0 357,711 - -0.5 357,752 + -0.3 357,752 + +0.7 357,753 - +0.3 357,796 + +1.0 357,919 + EX31_RS22975 0.10 -0.4 357,919 + EX31_RS22975 0.10 +0.3 357,922 + EX31_RS22975 0.10 +0.2 357,922 + EX31_RS22975 0.10 -1.0 357,922 + EX31_RS22975 0.10 +1.1 358,069 + EX31_RS22975 0.21 +0.5 358,069 + EX31_RS22975 0.21 -1.5 358,070 - EX31_RS22975 0.21 +1.1 358,070 - EX31_RS22975 0.21 -0.2 358,070 - EX31_RS22975 0.21 +1.8 358,179 - EX31_RS22975 0.28 +1.8 358,179 - EX31_RS22975 0.28 -0.8 358,229 + EX31_RS22975 0.32 -0.3 358,229 + EX31_RS22975 0.32 +0.6 358,230 - EX31_RS22975 0.32 +0.8 358,230 - EX31_RS22975 0.32 +1.6 358,230 - EX31_RS22975 0.32 -0.2
Or see this region's nucleotide sequence