Strain Fitness in Rahnella sp. WP5 around EX31_RS17665

Experiment: L-Glutamine (N); RCH2 media; shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS17660 and EX31_RS17665 are separated by 10 nucleotidesEX31_RS17665 and EX31_RS17670 are separated by 230 nucleotides EX31_RS17660: EX31_RS17660 - alpha/beta hydrolase, at 82,355 to 83,383 _RS17660 EX31_RS17665: EX31_RS17665 - MFS transporter, at 83,394 to 84,590 _RS17665 EX31_RS17670: EX31_RS17670 - LysR family transcriptional regulator, at 84,821 to 85,711 _RS17670 Position (kb) 83 84 85Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 82.419 kb on + strandat 82.452 kb on + strandat 82.452 kb on + strandat 82.452 kb on + strandat 82.452 kb on + strandat 82.452 kb on + strandat 82.452 kb on + strandat 82.453 kb on - strandat 82.453 kb on - strandat 82.453 kb on - strandat 82.453 kb on - strandat 82.453 kb on - strandat 82.453 kb on - strandat 82.453 kb on - strandat 82.453 kb on - strandat 82.453 kb on - strandat 82.453 kb on - strandat 82.453 kb on - strandat 82.482 kb on + strand, within EX31_RS17660at 82.482 kb on + strand, within EX31_RS17660at 82.648 kb on + strand, within EX31_RS17660at 82.648 kb on + strand, within EX31_RS17660at 82.648 kb on + strand, within EX31_RS17660at 82.738 kb on + strand, within EX31_RS17660at 82.740 kb on + strand, within EX31_RS17660at 82.740 kb on + strand, within EX31_RS17660at 82.740 kb on + strand, within EX31_RS17660at 82.741 kb on - strand, within EX31_RS17660at 82.741 kb on - strand, within EX31_RS17660at 82.741 kb on - strand, within EX31_RS17660at 82.783 kb on + strand, within EX31_RS17660at 82.784 kb on - strand, within EX31_RS17660at 82.792 kb on + strand, within EX31_RS17660at 82.793 kb on - strand, within EX31_RS17660at 82.863 kb on + strand, within EX31_RS17660at 82.864 kb on - strand, within EX31_RS17660at 82.864 kb on - strand, within EX31_RS17660at 82.864 kb on - strand, within EX31_RS17660at 82.864 kb on - strand, within EX31_RS17660at 82.975 kb on - strand, within EX31_RS17660at 82.975 kb on - strand, within EX31_RS17660at 82.975 kb on - strand, within EX31_RS17660at 82.975 kb on - strand, within EX31_RS17660at 82.975 kb on - strand, within EX31_RS17660at 82.992 kb on + strand, within EX31_RS17660at 82.992 kb on + strand, within EX31_RS17660at 83.009 kb on - strand, within EX31_RS17660at 83.129 kb on + strand, within EX31_RS17660at 83.129 kb on + strand, within EX31_RS17660at 83.130 kb on - strand, within EX31_RS17660at 83.130 kb on - strand, within EX31_RS17660at 83.130 kb on - strand, within EX31_RS17660at 83.217 kb on - strand, within EX31_RS17660at 83.248 kb on - strand, within EX31_RS17660at 83.284 kb on + strandat 83.375 kb on + strandat 83.375 kb on + strandat 83.375 kb on + strandat 83.375 kb on + strandat 83.375 kb on + strandat 83.376 kb on - strandat 83.376 kb on - strandat 83.376 kb on - strandat 83.376 kb on - strandat 83.376 kb on - strandat 83.412 kb on - strandat 83.417 kb on + strandat 83.417 kb on + strandat 83.418 kb on - strandat 83.418 kb on - strandat 83.495 kb on + strandat 83.495 kb on + strandat 83.561 kb on + strand, within EX31_RS17665at 83.561 kb on + strand, within EX31_RS17665at 83.561 kb on + strand, within EX31_RS17665at 83.561 kb on + strand, within EX31_RS17665at 83.679 kb on + strand, within EX31_RS17665at 83.679 kb on + strand, within EX31_RS17665at 83.680 kb on - strand, within EX31_RS17665at 83.680 kb on - strand, within EX31_RS17665at 83.680 kb on - strand, within EX31_RS17665at 83.680 kb on - strand, within EX31_RS17665at 83.741 kb on + strand, within EX31_RS17665at 83.843 kb on + strand, within EX31_RS17665at 83.843 kb on + strand, within EX31_RS17665at 83.843 kb on + strand, within EX31_RS17665at 83.844 kb on - strand, within EX31_RS17665at 83.844 kb on - strand, within EX31_RS17665at 83.847 kb on + strand, within EX31_RS17665at 83.848 kb on - strand, within EX31_RS17665at 83.882 kb on - strand, within EX31_RS17665at 83.889 kb on + strand, within EX31_RS17665at 84.030 kb on + strand, within EX31_RS17665at 84.031 kb on - strand, within EX31_RS17665at 84.031 kb on - strand, within EX31_RS17665at 84.031 kb on - strand, within EX31_RS17665at 84.031 kb on - strand, within EX31_RS17665at 84.031 kb on - strand, within EX31_RS17665at 84.031 kb on - strand, within EX31_RS17665at 84.032 kb on + strand, within EX31_RS17665at 84.032 kb on + strand, within EX31_RS17665at 84.033 kb on - strand, within EX31_RS17665at 84.033 kb on - strand, within EX31_RS17665at 84.033 kb on - strand, within EX31_RS17665at 84.120 kb on + strand, within EX31_RS17665at 84.120 kb on + strand, within EX31_RS17665at 84.121 kb on - strand, within EX31_RS17665at 84.265 kb on + strand, within EX31_RS17665at 84.265 kb on + strand, within EX31_RS17665at 84.266 kb on - strand, within EX31_RS17665at 84.266 kb on - strand, within EX31_RS17665at 84.340 kb on - strand, within EX31_RS17665at 84.340 kb on - strand, within EX31_RS17665at 84.447 kb on + strand, within EX31_RS17665at 84.447 kb on + strand, within EX31_RS17665at 84.448 kb on - strand, within EX31_RS17665at 84.448 kb on - strand, within EX31_RS17665at 84.486 kb on - strandat 84.486 kb on - strandat 84.486 kb on - strandat 84.544 kb on + strandat 84.570 kb on - strandat 84.590 kb on + strandat 84.591 kb on - strandat 84.591 kb on - strandat 84.591 kb on - strandat 84.591 kb on - strandat 84.591 kb on - strandat 84.595 kb on - strandat 84.597 kb on + strandat 84.598 kb on - strandat 84.598 kb on - strandat 84.618 kb on + strandat 84.619 kb on - strandat 84.653 kb on - strandat 84.658 kb on - strandat 84.678 kb on - strandat 84.692 kb on - strandat 84.718 kb on + strandat 84.719 kb on - strandat 84.760 kb on + strandat 84.848 kb on + strandat 84.856 kb on + strandat 84.953 kb on - strand, within EX31_RS17670at 84.976 kb on + strand, within EX31_RS17670at 84.976 kb on + strand, within EX31_RS17670at 84.977 kb on - strand, within EX31_RS17670at 85.050 kb on + strand, within EX31_RS17670at 85.050 kb on + strand, within EX31_RS17670at 85.062 kb on + strand, within EX31_RS17670at 85.062 kb on + strand, within EX31_RS17670at 85.280 kb on - strand, within EX31_RS17670at 85.284 kb on + strand, within EX31_RS17670at 85.284 kb on + strand, within EX31_RS17670at 85.284 kb on + strand, within EX31_RS17670at 85.284 kb on + strand, within EX31_RS17670at 85.285 kb on - strand, within EX31_RS17670at 85.285 kb on - strand, within EX31_RS17670at 85.337 kb on + strand, within EX31_RS17670at 85.351 kb on + strand, within EX31_RS17670at 85.351 kb on + strand, within EX31_RS17670at 85.396 kb on + strand, within EX31_RS17670at 85.396 kb on + strand, within EX31_RS17670at 85.399 kb on + strand, within EX31_RS17670at 85.427 kb on + strand, within EX31_RS17670at 85.428 kb on - strand, within EX31_RS17670at 85.428 kb on - strand, within EX31_RS17670at 85.461 kb on + strand, within EX31_RS17670at 85.461 kb on + strand, within EX31_RS17670at 85.495 kb on + strand, within EX31_RS17670at 85.573 kb on + strand, within EX31_RS17670

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking
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82,419 + +0.7
82,452 + +0.9
82,452 + -0.8
82,452 + +0.2
82,452 + +1.7
82,452 + -0.4
82,452 + +0.6
82,453 - +0.7
82,453 - +0.9
82,453 - -1.1
82,453 - -1.4
82,453 - -0.1
82,453 - +0.0
82,453 - +0.9
82,453 - -0.1
82,453 - +0.2
82,453 - -0.8
82,453 - +1.5
82,482 + EX31_RS17660 0.12 -0.6
82,482 + EX31_RS17660 0.12 +0.4
82,648 + EX31_RS17660 0.28 +0.7
82,648 + EX31_RS17660 0.28 -0.4
82,648 + EX31_RS17660 0.28 +4.9
82,738 + EX31_RS17660 0.37 +0.7
82,740 + EX31_RS17660 0.37 +0.3
82,740 + EX31_RS17660 0.37 +0.6
82,740 + EX31_RS17660 0.37 +0.2
82,741 - EX31_RS17660 0.38 -0.3
82,741 - EX31_RS17660 0.38 -0.1
82,741 - EX31_RS17660 0.38 -1.3
82,783 + EX31_RS17660 0.42 +1.4
82,784 - EX31_RS17660 0.42 +0.5
82,792 + EX31_RS17660 0.42 -1.7
82,793 - EX31_RS17660 0.43 -0.3
82,863 + EX31_RS17660 0.49 +0.7
82,864 - EX31_RS17660 0.49 -0.8
82,864 - EX31_RS17660 0.49 +0.9
82,864 - EX31_RS17660 0.49 +0.6
82,864 - EX31_RS17660 0.49 -0.4
82,975 - EX31_RS17660 0.60 +0.4
82,975 - EX31_RS17660 0.60 -1.7
82,975 - EX31_RS17660 0.60 -1.2
82,975 - EX31_RS17660 0.60 +1.1
82,975 - EX31_RS17660 0.60 +1.1
82,992 + EX31_RS17660 0.62 -0.5
82,992 + EX31_RS17660 0.62 +0.4
83,009 - EX31_RS17660 0.64 -0.7
83,129 + EX31_RS17660 0.75 +0.4
83,129 + EX31_RS17660 0.75 -0.8
83,130 - EX31_RS17660 0.75 +2.0
83,130 - EX31_RS17660 0.75 -0.3
83,130 - EX31_RS17660 0.75 +0.2
83,217 - EX31_RS17660 0.84 -0.3
83,248 - EX31_RS17660 0.87 +2.1
83,284 + +0.2
83,375 + +2.4
83,375 + +0.7
83,375 + -0.2
83,375 + -1.3
83,375 + -1.2
83,376 - +0.7
83,376 - -0.4
83,376 - +0.6
83,376 - -0.4
83,376 - -1.8
83,412 - +0.0
83,417 + -1.2
83,417 + -0.7
83,418 - +0.2
83,418 - -1.3
83,495 + +0.4
83,495 + -0.6
83,561 + EX31_RS17665 0.14 -1.1
83,561 + EX31_RS17665 0.14 +2.1
83,561 + EX31_RS17665 0.14 +0.6
83,561 + EX31_RS17665 0.14 +0.9
83,679 + EX31_RS17665 0.24 +0.0
83,679 + EX31_RS17665 0.24 +1.7
83,680 - EX31_RS17665 0.24 +0.3
83,680 - EX31_RS17665 0.24 -0.5
83,680 - EX31_RS17665 0.24 +1.4
83,680 - EX31_RS17665 0.24 +0.7
83,741 + EX31_RS17665 0.29 +1.0
83,843 + EX31_RS17665 0.38 -0.3
83,843 + EX31_RS17665 0.38 -0.1
83,843 + EX31_RS17665 0.38 +1.5
83,844 - EX31_RS17665 0.38 +2.0
83,844 - EX31_RS17665 0.38 -1.2
83,847 + EX31_RS17665 0.38 +0.8
83,848 - EX31_RS17665 0.38 +1.7
83,882 - EX31_RS17665 0.41 +1.7
83,889 + EX31_RS17665 0.41 +1.0
84,030 + EX31_RS17665 0.53 +0.7
84,031 - EX31_RS17665 0.53 -0.6
84,031 - EX31_RS17665 0.53 -0.5
84,031 - EX31_RS17665 0.53 +0.2
84,031 - EX31_RS17665 0.53 -0.3
84,031 - EX31_RS17665 0.53 -0.4
84,031 - EX31_RS17665 0.53 -1.3
84,032 + EX31_RS17665 0.53 +0.9
84,032 + EX31_RS17665 0.53 -1.3
84,033 - EX31_RS17665 0.53 +2.1
84,033 - EX31_RS17665 0.53 +1.2
84,033 - EX31_RS17665 0.53 +0.5
84,120 + EX31_RS17665 0.61 -0.2
84,120 + EX31_RS17665 0.61 +0.4
84,121 - EX31_RS17665 0.61 +0.8
84,265 + EX31_RS17665 0.73 +1.0
84,265 + EX31_RS17665 0.73 +1.0
84,266 - EX31_RS17665 0.73 -0.4
84,266 - EX31_RS17665 0.73 +1.1
84,340 - EX31_RS17665 0.79 +1.0
84,340 - EX31_RS17665 0.79 -0.1
84,447 + EX31_RS17665 0.88 -1.4
84,447 + EX31_RS17665 0.88 +1.3
84,448 - EX31_RS17665 0.88 -1.7
84,448 - EX31_RS17665 0.88 +0.0
84,486 - +0.1
84,486 - -1.3
84,486 - -2.4
84,544 + +0.9
84,570 - +0.1
84,590 + -1.4
84,591 - +1.2
84,591 - +1.5
84,591 - -0.6
84,591 - -1.1
84,591 - -0.2
84,595 - -0.6
84,597 + +0.4
84,598 - +1.7
84,598 - -0.8
84,618 + -0.7
84,619 - +0.4
84,653 - +1.1
84,658 - +0.5
84,678 - +0.9
84,692 - -0.8
84,718 + -2.2
84,719 - -0.1
84,760 + -0.5
84,848 + +0.1
84,856 + +0.3
84,953 - EX31_RS17670 0.15 -0.6
84,976 + EX31_RS17670 0.17 -0.3
84,976 + EX31_RS17670 0.17 +1.3
84,977 - EX31_RS17670 0.18 +0.2
85,050 + EX31_RS17670 0.26 +1.0
85,050 + EX31_RS17670 0.26 -0.5
85,062 + EX31_RS17670 0.27 -0.3
85,062 + EX31_RS17670 0.27 +0.6
85,280 - EX31_RS17670 0.52 -0.4
85,284 + EX31_RS17670 0.52 +1.6
85,284 + EX31_RS17670 0.52 +1.2
85,284 + EX31_RS17670 0.52 -0.1
85,284 + EX31_RS17670 0.52 -0.6
85,285 - EX31_RS17670 0.52 +2.1
85,285 - EX31_RS17670 0.52 -1.5
85,337 + EX31_RS17670 0.58 -1.7
85,351 + EX31_RS17670 0.59 -1.6
85,351 + EX31_RS17670 0.59 +0.3
85,396 + EX31_RS17670 0.65 +0.7
85,396 + EX31_RS17670 0.65 -2.4
85,399 + EX31_RS17670 0.65 +0.1
85,427 + EX31_RS17670 0.68 +1.0
85,428 - EX31_RS17670 0.68 +0.7
85,428 - EX31_RS17670 0.68 +1.3
85,461 + EX31_RS17670 0.72 +2.2
85,461 + EX31_RS17670 0.72 +0.5
85,495 + EX31_RS17670 0.76 +0.3
85,573 + EX31_RS17670 0.84 +0.7

Or see this region's nucleotide sequence