Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS13480 and EX31_RS13485 are separated by 99 nucleotides EX31_RS13485 and EX31_RS13490 are separated by 45 nucleotides EX31_RS13490 and EX31_RS13495 overlap by 20 nucleotides EX31_RS13495 and EX31_RS13500 are separated by 278 nucleotides
EX31_RS13480: EX31_RS13480 - site-specific tyrosine recombinase XerD, at 131,046 to 132,005
_RS13480
EX31_RS13485: EX31_RS13485 - flavodoxin FldB, at 132,105 to 132,623
_RS13485
EX31_RS13490: EX31_RS13490 - membrane protein, at 132,669 to 133,121
_RS13490
EX31_RS13495: EX31_RS13495 - FAD assembly factor SdhE, at 133,102 to 133,368
_RS13495
EX31_RS13500: EX31_RS13500 - tRNA-modifying protein YgfZ, at 133,647 to 134,639
_RS13500
Position (kb) 132
133
134 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 131.718 kb on + strand, within EX31_RS13480 at 131.718 kb on + strand, within EX31_RS13480 at 131.719 kb on - strand, within EX31_RS13480 at 131.719 kb on - strand, within EX31_RS13480 at 131.719 kb on - strand, within EX31_RS13480 at 131.719 kb on - strand, within EX31_RS13480 at 131.719 kb on - strand, within EX31_RS13480 at 131.757 kb on - strand, within EX31_RS13480 at 131.778 kb on + strand, within EX31_RS13480 at 131.816 kb on + strand, within EX31_RS13480 at 131.817 kb on - strand, within EX31_RS13480 at 131.817 kb on - strand, within EX31_RS13480 at 131.904 kb on - strand, within EX31_RS13480 at 132.018 kb on + strand at 132.019 kb on - strand at 132.034 kb on + strand at 132.034 kb on + strand at 132.034 kb on + strand at 132.034 kb on + strand at 132.034 kb on + strand at 132.035 kb on - strand at 132.061 kb on - strand at 132.078 kb on + strand at 132.078 kb on + strand at 132.079 kb on - strand at 132.092 kb on + strand at 132.104 kb on + strand at 132.124 kb on - strand at 132.141 kb on + strand at 132.141 kb on + strand at 132.141 kb on + strand at 132.204 kb on - strand, within EX31_RS13485 at 132.208 kb on + strand, within EX31_RS13485 at 132.208 kb on + strand, within EX31_RS13485 at 132.208 kb on + strand, within EX31_RS13485 at 132.208 kb on + strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.209 kb on - strand, within EX31_RS13485 at 132.240 kb on + strand, within EX31_RS13485 at 132.240 kb on + strand, within EX31_RS13485 at 132.241 kb on - strand, within EX31_RS13485 at 132.241 kb on - strand, within EX31_RS13485 at 132.241 kb on - strand, within EX31_RS13485 at 132.241 kb on - strand, within EX31_RS13485 at 132.241 kb on - strand, within EX31_RS13485 at 132.334 kb on + strand, within EX31_RS13485 at 132.357 kb on + strand, within EX31_RS13485 at 132.417 kb on + strand, within EX31_RS13485 at 132.482 kb on + strand, within EX31_RS13485 at 132.631 kb on + strand at 132.632 kb on - strand at 132.632 kb on - strand at 132.632 kb on - strand at 132.690 kb on - strand at 132.711 kb on + strand at 132.711 kb on + strand at 132.711 kb on + strand at 132.809 kb on + strand, within EX31_RS13490 at 132.837 kb on + strand, within EX31_RS13490 at 132.838 kb on - strand, within EX31_RS13490 at 132.838 kb on - strand, within EX31_RS13490 at 132.838 kb on - strand, within EX31_RS13490 at 132.849 kb on + strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.850 kb on - strand, within EX31_RS13490 at 132.858 kb on + strand, within EX31_RS13490 at 132.894 kb on + strand, within EX31_RS13490 at 132.894 kb on + strand, within EX31_RS13490 at 132.895 kb on - strand, within EX31_RS13490 at 132.942 kb on - strand, within EX31_RS13490 at 132.942 kb on - strand, within EX31_RS13490 at 132.944 kb on + strand, within EX31_RS13490 at 132.944 kb on + strand, within EX31_RS13490 at 132.945 kb on - strand, within EX31_RS13490 at 132.945 kb on - strand, within EX31_RS13490 at 133.058 kb on - strand, within EX31_RS13490 at 133.073 kb on + strand, within EX31_RS13490 at 133.074 kb on - strand, within EX31_RS13490 at 133.155 kb on + strand, within EX31_RS13495 at 133.155 kb on + strand, within EX31_RS13495 at 133.155 kb on + strand, within EX31_RS13495 at 133.156 kb on - strand, within EX31_RS13495 at 133.243 kb on + strand, within EX31_RS13495 at 133.243 kb on + strand, within EX31_RS13495 at 133.243 kb on + strand, within EX31_RS13495 at 133.244 kb on - strand, within EX31_RS13495 at 133.244 kb on - strand, within EX31_RS13495 at 133.244 kb on - strand, within EX31_RS13495 at 133.244 kb on - strand, within EX31_RS13495 at 133.274 kb on + strand, within EX31_RS13495 at 133.274 kb on + strand, within EX31_RS13495 at 133.274 kb on + strand, within EX31_RS13495 at 133.274 kb on + strand, within EX31_RS13495 at 133.275 kb on - strand, within EX31_RS13495 at 133.275 kb on - strand, within EX31_RS13495 at 133.275 kb on - strand, within EX31_RS13495 at 133.306 kb on - strand, within EX31_RS13495 at 133.359 kb on + strand at 133.359 kb on + strand at 133.360 kb on - strand at 133.360 kb on - strand at 133.363 kb on - strand at 133.426 kb on + strand at 133.426 kb on + strand at 133.426 kb on + strand at 133.426 kb on + strand at 133.430 kb on + strand at 133.430 kb on + strand at 133.430 kb on + strand at 133.430 kb on + strand at 133.430 kb on + strand at 133.430 kb on + strand at 133.430 kb on + strand at 133.430 kb on + strand at 133.430 kb on + strand at 133.430 kb on + strand at 133.430 kb on + strand at 133.430 kb on + strand at 133.430 kb on + strand at 133.431 kb on - strand at 133.431 kb on - strand at 133.431 kb on - strand at 133.431 kb on - strand at 133.431 kb on - strand at 133.431 kb on - strand at 133.431 kb on - strand at 133.431 kb on - strand at 133.431 kb on - strand at 133.431 kb on - strand at 133.443 kb on + strand at 133.443 kb on + strand at 133.443 kb on + strand at 133.443 kb on + strand at 133.444 kb on - strand at 133.466 kb on + strand at 133.466 kb on + strand at 133.539 kb on + strand at 133.540 kb on - strand at 133.575 kb on + strand at 133.575 kb on + strand at 133.576 kb on - strand at 133.576 kb on - strand at 133.604 kb on + strand at 133.604 kb on + strand at 133.604 kb on + strand at 133.604 kb on + strand at 133.605 kb on - strand at 133.605 kb on - strand at 133.605 kb on - strand at 133.605 kb on - strand at 133.605 kb on - strand at 133.605 kb on - strand at 133.627 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 131,718 + EX31_RS13480 0.70 +0.2 131,718 + EX31_RS13480 0.70 -1.3 131,719 - EX31_RS13480 0.70 +2.2 131,719 - EX31_RS13480 0.70 +0.8 131,719 - EX31_RS13480 0.70 -0.2 131,719 - EX31_RS13480 0.70 -0.4 131,719 - EX31_RS13480 0.70 -0.2 131,757 - EX31_RS13480 0.74 -2.0 131,778 + EX31_RS13480 0.76 -0.8 131,816 + EX31_RS13480 0.80 -0.9 131,817 - EX31_RS13480 0.80 +0.7 131,817 - EX31_RS13480 0.80 -0.4 131,904 - EX31_RS13480 0.89 +0.1 132,018 + -1.7 132,019 - +0.9 132,034 + +1.0 132,034 + +0.5 132,034 + +0.5 132,034 + +0.3 132,034 + +0.7 132,035 - -0.2 132,061 - -0.0 132,078 + +0.4 132,078 + +2.2 132,079 - -0.0 132,092 + -1.4 132,104 + +0.0 132,124 - -1.7 132,141 + +0.1 132,141 + -0.4 132,141 + -2.2 132,204 - EX31_RS13485 0.19 -0.1 132,208 + EX31_RS13485 0.20 +0.7 132,208 + EX31_RS13485 0.20 +1.0 132,208 + EX31_RS13485 0.20 -0.1 132,208 + EX31_RS13485 0.20 -1.2 132,209 - EX31_RS13485 0.20 +0.1 132,209 - EX31_RS13485 0.20 +2.5 132,209 - EX31_RS13485 0.20 +0.5 132,209 - EX31_RS13485 0.20 -1.1 132,209 - EX31_RS13485 0.20 +1.0 132,209 - EX31_RS13485 0.20 -1.5 132,209 - EX31_RS13485 0.20 +0.6 132,209 - EX31_RS13485 0.20 +0.9 132,209 - EX31_RS13485 0.20 +0.1 132,209 - EX31_RS13485 0.20 -1.0 132,240 + EX31_RS13485 0.26 +0.9 132,240 + EX31_RS13485 0.26 +3.2 132,241 - EX31_RS13485 0.26 +0.2 132,241 - EX31_RS13485 0.26 -1.0 132,241 - EX31_RS13485 0.26 +0.4 132,241 - EX31_RS13485 0.26 -0.4 132,241 - EX31_RS13485 0.26 +0.1 132,334 + EX31_RS13485 0.44 -0.8 132,357 + EX31_RS13485 0.49 -2.7 132,417 + EX31_RS13485 0.60 -1.1 132,482 + EX31_RS13485 0.73 +0.9 132,631 + +0.5 132,632 - -0.9 132,632 - +0.5 132,632 - -0.2 132,690 - +0.2 132,711 + +0.7 132,711 + +0.8 132,711 + +1.9 132,809 + EX31_RS13490 0.31 -1.1 132,837 + EX31_RS13490 0.37 +0.2 132,838 - EX31_RS13490 0.37 -0.1 132,838 - EX31_RS13490 0.37 -0.3 132,838 - EX31_RS13490 0.37 -0.4 132,849 + EX31_RS13490 0.40 +0.1 132,850 - EX31_RS13490 0.40 -1.4 132,850 - EX31_RS13490 0.40 -0.8 132,850 - EX31_RS13490 0.40 +0.0 132,850 - EX31_RS13490 0.40 -0.7 132,850 - EX31_RS13490 0.40 -0.2 132,850 - EX31_RS13490 0.40 -0.2 132,850 - EX31_RS13490 0.40 +0.2 132,850 - EX31_RS13490 0.40 +1.3 132,858 + EX31_RS13490 0.42 -0.5 132,894 + EX31_RS13490 0.50 -0.2 132,894 + EX31_RS13490 0.50 +0.5 132,895 - EX31_RS13490 0.50 +0.0 132,942 - EX31_RS13490 0.60 -0.3 132,942 - EX31_RS13490 0.60 +0.3 132,944 + EX31_RS13490 0.61 +0.2 132,944 + EX31_RS13490 0.61 +0.4 132,945 - EX31_RS13490 0.61 -0.5 132,945 - EX31_RS13490 0.61 +1.2 133,058 - EX31_RS13490 0.86 -0.8 133,073 + EX31_RS13490 0.89 +0.3 133,074 - EX31_RS13490 0.89 -0.8 133,155 + EX31_RS13495 0.20 -0.1 133,155 + EX31_RS13495 0.20 +0.2 133,155 + EX31_RS13495 0.20 +0.2 133,156 - EX31_RS13495 0.20 +0.1 133,243 + EX31_RS13495 0.53 -1.8 133,243 + EX31_RS13495 0.53 -1.3 133,243 + EX31_RS13495 0.53 -0.0 133,244 - EX31_RS13495 0.53 +0.2 133,244 - EX31_RS13495 0.53 -0.8 133,244 - EX31_RS13495 0.53 -0.5 133,244 - EX31_RS13495 0.53 -0.2 133,274 + EX31_RS13495 0.64 +0.2 133,274 + EX31_RS13495 0.64 +0.9 133,274 + EX31_RS13495 0.64 -0.1 133,274 + EX31_RS13495 0.64 -1.5 133,275 - EX31_RS13495 0.65 -0.5 133,275 - EX31_RS13495 0.65 +0.6 133,275 - EX31_RS13495 0.65 -1.0 133,306 - EX31_RS13495 0.76 -1.5 133,359 + -0.2 133,359 + -0.1 133,360 - -0.5 133,360 - -1.0 133,363 - -1.4 133,426 + -0.7 133,426 + +1.6 133,426 + -0.3 133,426 + +0.7 133,430 + -0.2 133,430 + +0.7 133,430 + -0.1 133,430 + +0.6 133,430 + +0.1 133,430 + +0.3 133,430 + +0.4 133,430 + +0.3 133,430 + -0.0 133,430 + +0.2 133,430 + -1.8 133,430 + +1.4 133,430 + +0.5 133,431 - +1.9 133,431 - -0.3 133,431 - +0.3 133,431 - +1.7 133,431 - +2.2 133,431 - -0.2 133,431 - +0.9 133,431 - +0.4 133,431 - +0.4 133,431 - -1.8 133,443 + +0.1 133,443 + +1.3 133,443 + -1.1 133,443 + -0.7 133,444 - -0.2 133,466 + -0.8 133,466 + +0.2 133,539 + +0.3 133,540 - +0.0 133,575 + -0.5 133,575 + +0.9 133,576 - -1.3 133,576 - -0.7 133,604 + -1.2 133,604 + -0.0 133,604 + -1.5 133,604 + -1.0 133,605 - +0.8 133,605 - -0.0 133,605 - -0.5 133,605 - -0.2 133,605 - +0.0 133,605 - -1.5 133,627 + -0.5
Or see this region's nucleotide sequence