Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS08060 and EX31_RS08065 are separated by 332 nucleotides EX31_RS08065 and EX31_RS08070 are separated by 30 nucleotides EX31_RS08070 and EX31_RS08075 are separated by 83 nucleotides
EX31_RS08060: EX31_RS08060 - DNA topoisomerase IV subunit A, at 103,210 to 105,483
_RS08060
EX31_RS08065: EX31_RS08065 - NAD(P)H-dependent oxidoreductase, at 105,816 to 106,403
_RS08065
EX31_RS08070: EX31_RS08070 - antibiotic biosynthesis monooxygenase, at 106,434 to 106,739
_RS08070
EX31_RS08075: EX31_RS08075 - DNA topoisomerase IV subunit B, at 106,823 to 108,718
_RS08075
Position (kb) 105
106
107 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 105.592 kb on - strand at 105.592 kb on - strand at 105.592 kb on - strand at 105.592 kb on - strand at 105.592 kb on - strand at 105.592 kb on - strand at 105.592 kb on - strand at 105.592 kb on - strand at 105.592 kb on - strand at 105.592 kb on - strand at 105.592 kb on - strand at 105.592 kb on - strand at 105.606 kb on - strand at 105.606 kb on - strand at 105.606 kb on - strand at 105.637 kb on - strand at 105.712 kb on - strand at 105.756 kb on + strand at 105.756 kb on + strand at 105.756 kb on + strand at 105.756 kb on + strand at 105.756 kb on + strand at 105.756 kb on + strand at 105.757 kb on - strand at 105.757 kb on - strand at 105.757 kb on - strand at 105.757 kb on - strand at 105.761 kb on + strand at 105.761 kb on + strand at 105.761 kb on + strand at 105.761 kb on + strand at 105.761 kb on + strand at 105.761 kb on + strand at 105.761 kb on + strand at 105.762 kb on - strand at 105.768 kb on + strand at 105.768 kb on + strand at 105.768 kb on + strand at 105.769 kb on - strand at 105.769 kb on - strand at 105.790 kb on + strand at 105.791 kb on - strand at 105.791 kb on - strand at 105.832 kb on + strand at 105.957 kb on + strand, within EX31_RS08065 at 105.984 kb on + strand, within EX31_RS08065 at 105.984 kb on + strand, within EX31_RS08065 at 105.984 kb on + strand, within EX31_RS08065 at 105.984 kb on + strand, within EX31_RS08065 at 105.984 kb on + strand, within EX31_RS08065 at 105.984 kb on + strand, within EX31_RS08065 at 105.984 kb on + strand, within EX31_RS08065 at 105.985 kb on - strand, within EX31_RS08065 at 105.985 kb on - strand, within EX31_RS08065 at 105.985 kb on - strand, within EX31_RS08065 at 105.985 kb on - strand, within EX31_RS08065 at 105.985 kb on - strand, within EX31_RS08065 at 105.985 kb on - strand, within EX31_RS08065 at 106.008 kb on + strand, within EX31_RS08065 at 106.008 kb on + strand, within EX31_RS08065 at 106.009 kb on - strand, within EX31_RS08065 at 106.009 kb on - strand, within EX31_RS08065 at 106.057 kb on - strand, within EX31_RS08065 at 106.088 kb on + strand, within EX31_RS08065 at 106.088 kb on + strand, within EX31_RS08065 at 106.089 kb on - strand, within EX31_RS08065 at 106.095 kb on + strand, within EX31_RS08065 at 106.096 kb on - strand, within EX31_RS08065 at 106.096 kb on - strand, within EX31_RS08065 at 106.112 kb on + strand, within EX31_RS08065 at 106.137 kb on + strand, within EX31_RS08065 at 106.170 kb on + strand, within EX31_RS08065 at 106.170 kb on + strand, within EX31_RS08065 at 106.170 kb on + strand, within EX31_RS08065 at 106.170 kb on + strand, within EX31_RS08065 at 106.170 kb on + strand, within EX31_RS08065 at 106.170 kb on + strand, within EX31_RS08065 at 106.171 kb on - strand, within EX31_RS08065 at 106.171 kb on - strand, within EX31_RS08065 at 106.171 kb on - strand, within EX31_RS08065 at 106.171 kb on - strand, within EX31_RS08065 at 106.171 kb on - strand, within EX31_RS08065 at 106.171 kb on - strand, within EX31_RS08065 at 106.171 kb on - strand, within EX31_RS08065 at 106.217 kb on + strand, within EX31_RS08065 at 106.218 kb on - strand, within EX31_RS08065 at 106.240 kb on + strand, within EX31_RS08065 at 106.255 kb on + strand, within EX31_RS08065 at 106.255 kb on + strand, within EX31_RS08065 at 106.255 kb on + strand, within EX31_RS08065 at 106.255 kb on + strand, within EX31_RS08065 at 106.255 kb on + strand, within EX31_RS08065 at 106.255 kb on + strand, within EX31_RS08065 at 106.256 kb on - strand, within EX31_RS08065 at 106.256 kb on - strand, within EX31_RS08065 at 106.256 kb on - strand, within EX31_RS08065 at 106.256 kb on - strand, within EX31_RS08065 at 106.256 kb on - strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.257 kb on + strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.258 kb on - strand, within EX31_RS08065 at 106.298 kb on + strand, within EX31_RS08065 at 106.298 kb on + strand, within EX31_RS08065 at 106.298 kb on + strand, within EX31_RS08065 at 106.298 kb on + strand, within EX31_RS08065 at 106.299 kb on - strand, within EX31_RS08065 at 106.299 kb on - strand, within EX31_RS08065 at 106.299 kb on - strand, within EX31_RS08065 at 106.299 kb on - strand, within EX31_RS08065 at 106.299 kb on - strand, within EX31_RS08065 at 106.299 kb on - strand, within EX31_RS08065 at 106.299 kb on - strand, within EX31_RS08065 at 106.299 kb on - strand, within EX31_RS08065 at 106.299 kb on - strand, within EX31_RS08065 at 106.299 kb on - strand, within EX31_RS08065 at 106.299 kb on - strand, within EX31_RS08065 at 106.299 kb on - strand, within EX31_RS08065 at 106.313 kb on + strand, within EX31_RS08065 at 106.313 kb on + strand, within EX31_RS08065 at 106.313 kb on + strand, within EX31_RS08065 at 106.313 kb on + strand, within EX31_RS08065 at 106.313 kb on + strand, within EX31_RS08065 at 106.314 kb on - strand, within EX31_RS08065 at 106.314 kb on - strand, within EX31_RS08065 at 106.314 kb on - strand, within EX31_RS08065 at 106.314 kb on - strand, within EX31_RS08065 at 106.314 kb on - strand, within EX31_RS08065 at 106.314 kb on - strand, within EX31_RS08065 at 106.314 kb on - strand, within EX31_RS08065 at 106.319 kb on + strand, within EX31_RS08065 at 106.320 kb on - strand, within EX31_RS08065 at 106.320 kb on - strand, within EX31_RS08065 at 106.320 kb on - strand, within EX31_RS08065 at 106.320 kb on - strand, within EX31_RS08065 at 106.387 kb on + strand at 106.387 kb on + strand at 106.388 kb on - strand at 106.388 kb on - strand at 106.388 kb on - strand at 106.401 kb on + strand at 106.401 kb on + strand at 106.402 kb on - strand at 106.402 kb on - strand at 106.402 kb on - strand at 106.402 kb on - strand at 106.402 kb on - strand at 106.434 kb on - strand at 106.445 kb on + strand at 106.445 kb on + strand at 106.446 kb on - strand at 106.446 kb on - strand at 106.462 kb on + strand at 106.463 kb on - strand at 106.489 kb on + strand, within EX31_RS08070 at 106.499 kb on + strand, within EX31_RS08070 at 106.499 kb on + strand, within EX31_RS08070 at 106.554 kb on + strand, within EX31_RS08070 at 106.554 kb on + strand, within EX31_RS08070 at 106.554 kb on + strand, within EX31_RS08070 at 106.554 kb on + strand, within EX31_RS08070 at 106.554 kb on + strand, within EX31_RS08070 at 106.554 kb on + strand, within EX31_RS08070 at 106.554 kb on + strand, within EX31_RS08070 at 106.554 kb on + strand, within EX31_RS08070 at 106.554 kb on + strand, within EX31_RS08070 at 106.555 kb on - strand, within EX31_RS08070 at 106.555 kb on - strand, within EX31_RS08070 at 106.555 kb on - strand, within EX31_RS08070 at 106.555 kb on - strand, within EX31_RS08070 at 106.555 kb on - strand, within EX31_RS08070 at 106.555 kb on - strand, within EX31_RS08070 at 106.555 kb on - strand, within EX31_RS08070 at 106.555 kb on - strand, within EX31_RS08070 at 106.555 kb on - strand, within EX31_RS08070 at 106.555 kb on - strand, within EX31_RS08070 at 106.555 kb on - strand, within EX31_RS08070 at 106.555 kb on - strand, within EX31_RS08070 at 106.555 kb on - strand, within EX31_RS08070 at 106.586 kb on + strand, within EX31_RS08070 at 106.586 kb on + strand, within EX31_RS08070 at 106.586 kb on + strand, within EX31_RS08070 at 106.587 kb on - strand, within EX31_RS08070 at 106.587 kb on - strand, within EX31_RS08070 at 106.595 kb on - strand, within EX31_RS08070 at 106.673 kb on + strand, within EX31_RS08070 at 106.673 kb on + strand, within EX31_RS08070 at 106.725 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 105,592 - +0.1 105,592 - -2.3 105,592 - -1.4 105,592 - +1.7 105,592 - -0.2 105,592 - +0.8 105,592 - +0.6 105,592 - +0.8 105,592 - +0.3 105,592 - +2.1 105,592 - +0.1 105,592 - -0.1 105,606 - +1.3 105,606 - +0.7 105,606 - +0.7 105,637 - -0.3 105,712 - -0.4 105,756 + +0.1 105,756 + -0.2 105,756 + -2.8 105,756 + -0.2 105,756 + +0.4 105,756 + -0.1 105,757 - +1.0 105,757 - -1.1 105,757 - +0.0 105,757 - +0.1 105,761 + +0.5 105,761 + +0.7 105,761 + +0.1 105,761 + -0.2 105,761 + -0.9 105,761 + +0.4 105,761 + -0.7 105,762 - -1.7 105,768 + +1.2 105,768 + -0.3 105,768 + -0.5 105,769 - +0.2 105,769 - -0.7 105,790 + +1.0 105,791 - -0.2 105,791 - -0.3 105,832 + -0.9 105,957 + EX31_RS08065 0.24 +0.0 105,984 + EX31_RS08065 0.29 -0.3 105,984 + EX31_RS08065 0.29 -0.9 105,984 + EX31_RS08065 0.29 +0.1 105,984 + EX31_RS08065 0.29 +0.1 105,984 + EX31_RS08065 0.29 -0.7 105,984 + EX31_RS08065 0.29 +0.8 105,984 + EX31_RS08065 0.29 +0.4 105,985 - EX31_RS08065 0.29 +0.5 105,985 - EX31_RS08065 0.29 -1.5 105,985 - EX31_RS08065 0.29 +0.9 105,985 - EX31_RS08065 0.29 +1.1 105,985 - EX31_RS08065 0.29 -0.7 105,985 - EX31_RS08065 0.29 -0.2 106,008 + EX31_RS08065 0.33 +0.5 106,008 + EX31_RS08065 0.33 +0.7 106,009 - EX31_RS08065 0.33 +1.1 106,009 - EX31_RS08065 0.33 -0.2 106,057 - EX31_RS08065 0.41 +0.7 106,088 + EX31_RS08065 0.46 +0.3 106,088 + EX31_RS08065 0.46 +0.1 106,089 - EX31_RS08065 0.46 +0.6 106,095 + EX31_RS08065 0.47 +0.6 106,096 - EX31_RS08065 0.48 +0.2 106,096 - EX31_RS08065 0.48 -0.1 106,112 + EX31_RS08065 0.50 -1.1 106,137 + EX31_RS08065 0.55 -0.5 106,170 + EX31_RS08065 0.60 +0.7 106,170 + EX31_RS08065 0.60 +0.1 106,170 + EX31_RS08065 0.60 -0.1 106,170 + EX31_RS08065 0.60 +1.2 106,170 + EX31_RS08065 0.60 -0.8 106,170 + EX31_RS08065 0.60 +1.1 106,171 - EX31_RS08065 0.60 +0.4 106,171 - EX31_RS08065 0.60 +0.2 106,171 - EX31_RS08065 0.60 -0.9 106,171 - EX31_RS08065 0.60 +0.1 106,171 - EX31_RS08065 0.60 +0.9 106,171 - EX31_RS08065 0.60 +0.0 106,171 - EX31_RS08065 0.60 -0.5 106,217 + EX31_RS08065 0.68 -0.9 106,218 - EX31_RS08065 0.68 +0.6 106,240 + EX31_RS08065 0.72 +0.2 106,255 + EX31_RS08065 0.75 +0.5 106,255 + EX31_RS08065 0.75 -0.3 106,255 + EX31_RS08065 0.75 +0.5 106,255 + EX31_RS08065 0.75 -0.1 106,255 + EX31_RS08065 0.75 -0.1 106,255 + EX31_RS08065 0.75 +0.2 106,256 - EX31_RS08065 0.75 +0.3 106,256 - EX31_RS08065 0.75 +1.8 106,256 - EX31_RS08065 0.75 +0.7 106,256 - EX31_RS08065 0.75 +2.3 106,256 - EX31_RS08065 0.75 +0.8 106,257 + EX31_RS08065 0.75 +1.1 106,257 + EX31_RS08065 0.75 -1.9 106,257 + EX31_RS08065 0.75 -0.6 106,257 + EX31_RS08065 0.75 +0.6 106,257 + EX31_RS08065 0.75 +0.5 106,257 + EX31_RS08065 0.75 -0.2 106,257 + EX31_RS08065 0.75 +0.7 106,257 + EX31_RS08065 0.75 +0.7 106,257 + EX31_RS08065 0.75 +1.1 106,257 + EX31_RS08065 0.75 -0.5 106,257 + EX31_RS08065 0.75 +0.1 106,257 + EX31_RS08065 0.75 -0.4 106,257 + EX31_RS08065 0.75 +0.2 106,257 + EX31_RS08065 0.75 +1.2 106,257 + EX31_RS08065 0.75 +0.2 106,257 + EX31_RS08065 0.75 -0.7 106,257 + EX31_RS08065 0.75 +0.7 106,258 - EX31_RS08065 0.75 +1.4 106,258 - EX31_RS08065 0.75 -0.7 106,258 - EX31_RS08065 0.75 -0.1 106,258 - EX31_RS08065 0.75 -0.3 106,258 - EX31_RS08065 0.75 -1.0 106,258 - EX31_RS08065 0.75 -1.5 106,258 - EX31_RS08065 0.75 -0.1 106,258 - EX31_RS08065 0.75 +0.3 106,258 - EX31_RS08065 0.75 +1.7 106,258 - EX31_RS08065 0.75 +0.7 106,258 - EX31_RS08065 0.75 +0.3 106,258 - EX31_RS08065 0.75 +0.6 106,258 - EX31_RS08065 0.75 -0.1 106,258 - EX31_RS08065 0.75 +0.6 106,258 - EX31_RS08065 0.75 +0.2 106,258 - EX31_RS08065 0.75 +0.2 106,258 - EX31_RS08065 0.75 -1.7 106,258 - EX31_RS08065 0.75 +0.5 106,258 - EX31_RS08065 0.75 -1.7 106,258 - EX31_RS08065 0.75 -0.4 106,258 - EX31_RS08065 0.75 +0.6 106,258 - EX31_RS08065 0.75 +1.3 106,258 - EX31_RS08065 0.75 -0.2 106,298 + EX31_RS08065 0.82 +0.3 106,298 + EX31_RS08065 0.82 -0.7 106,298 + EX31_RS08065 0.82 +0.8 106,298 + EX31_RS08065 0.82 +0.1 106,299 - EX31_RS08065 0.82 -0.1 106,299 - EX31_RS08065 0.82 -1.3 106,299 - EX31_RS08065 0.82 +0.9 106,299 - EX31_RS08065 0.82 +0.1 106,299 - EX31_RS08065 0.82 +0.7 106,299 - EX31_RS08065 0.82 +0.3 106,299 - EX31_RS08065 0.82 -0.9 106,299 - EX31_RS08065 0.82 -2.4 106,299 - EX31_RS08065 0.82 -1.5 106,299 - EX31_RS08065 0.82 +1.7 106,299 - EX31_RS08065 0.82 -0.2 106,299 - EX31_RS08065 0.82 -1.9 106,313 + EX31_RS08065 0.85 +0.1 106,313 + EX31_RS08065 0.85 +0.3 106,313 + EX31_RS08065 0.85 -0.6 106,313 + EX31_RS08065 0.85 +0.5 106,313 + EX31_RS08065 0.85 -0.1 106,314 - EX31_RS08065 0.85 +0.5 106,314 - EX31_RS08065 0.85 +1.5 106,314 - EX31_RS08065 0.85 +0.0 106,314 - EX31_RS08065 0.85 -0.3 106,314 - EX31_RS08065 0.85 -0.5 106,314 - EX31_RS08065 0.85 +0.4 106,314 - EX31_RS08065 0.85 +1.6 106,319 + EX31_RS08065 0.86 +1.1 106,320 - EX31_RS08065 0.86 -0.0 106,320 - EX31_RS08065 0.86 -1.4 106,320 - EX31_RS08065 0.86 -0.8 106,320 - EX31_RS08065 0.86 +0.4 106,387 + -0.6 106,387 + +0.7 106,388 - -0.8 106,388 - +0.5 106,388 - +0.5 106,401 + -0.4 106,401 + -0.2 106,402 - +0.6 106,402 - +1.4 106,402 - +0.5 106,402 - +0.0 106,402 - -0.3 106,434 - -1.4 106,445 + +0.4 106,445 + +1.1 106,446 - -0.4 106,446 - +0.2 106,462 + +0.4 106,463 - +0.4 106,489 + EX31_RS08070 0.18 +1.4 106,499 + EX31_RS08070 0.21 +0.4 106,499 + EX31_RS08070 0.21 +0.2 106,554 + EX31_RS08070 0.39 -0.2 106,554 + EX31_RS08070 0.39 +0.7 106,554 + EX31_RS08070 0.39 +0.5 106,554 + EX31_RS08070 0.39 -0.4 106,554 + EX31_RS08070 0.39 +0.1 106,554 + EX31_RS08070 0.39 -0.4 106,554 + EX31_RS08070 0.39 -0.4 106,554 + EX31_RS08070 0.39 -1.3 106,554 + EX31_RS08070 0.39 +1.4 106,555 - EX31_RS08070 0.40 +0.4 106,555 - EX31_RS08070 0.40 +0.3 106,555 - EX31_RS08070 0.40 -0.5 106,555 - EX31_RS08070 0.40 +0.8 106,555 - EX31_RS08070 0.40 +1.1 106,555 - EX31_RS08070 0.40 +0.6 106,555 - EX31_RS08070 0.40 -0.2 106,555 - EX31_RS08070 0.40 +1.1 106,555 - EX31_RS08070 0.40 -1.9 106,555 - EX31_RS08070 0.40 +1.3 106,555 - EX31_RS08070 0.40 +1.3 106,555 - EX31_RS08070 0.40 +0.5 106,555 - EX31_RS08070 0.40 -0.5 106,586 + EX31_RS08070 0.50 -0.0 106,586 + EX31_RS08070 0.50 -1.6 106,586 + EX31_RS08070 0.50 +1.3 106,587 - EX31_RS08070 0.50 +0.3 106,587 - EX31_RS08070 0.50 -0.3 106,595 - EX31_RS08070 0.53 -1.1 106,673 + EX31_RS08070 0.78 +0.2 106,673 + EX31_RS08070 0.78 +0.1 106,725 - +0.6
Or see this region's nucleotide sequence