Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00835 and EX31_RS00840 overlap by 8 nucleotides EX31_RS00840 and EX31_RS00845 are separated by 48 nucleotides EX31_RS00845 and EX31_RS00850 are separated by 534 nucleotides
EX31_RS00835: EX31_RS00835 - branched-chain amino acid ABC transporter permease, at 181,425 to 182,312
_RS00835
EX31_RS00840: EX31_RS00840 - branched-chain amino acid ABC transporter permease, at 182,305 to 183,198
_RS00840
EX31_RS00845: EX31_RS00845 - ABC transporter substrate-binding protein, at 183,247 to 184,374
_RS00845
EX31_RS00850: EX31_RS00850 - GntR family transcriptional regulator, at 184,909 to 185,610
_RS00850
Position (kb)
183
184
185 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 182.269 kb on + strand at 182.269 kb on + strand at 182.270 kb on - strand at 182.291 kb on + strand at 182.291 kb on + strand at 182.350 kb on + strand at 182.351 kb on - strand at 182.351 kb on - strand at 182.351 kb on - strand at 182.413 kb on + strand, within EX31_RS00840 at 182.414 kb on - strand, within EX31_RS00840 at 182.598 kb on + strand, within EX31_RS00840 at 182.598 kb on + strand, within EX31_RS00840 at 182.598 kb on + strand, within EX31_RS00840 at 182.649 kb on + strand, within EX31_RS00840 at 182.650 kb on - strand, within EX31_RS00840 at 182.849 kb on + strand, within EX31_RS00840 at 182.850 kb on - strand, within EX31_RS00840 at 182.906 kb on - strand, within EX31_RS00840 at 183.064 kb on - strand, within EX31_RS00840 at 183.083 kb on + strand, within EX31_RS00840 at 183.084 kb on - strand, within EX31_RS00840 at 183.091 kb on - strand, within EX31_RS00840 at 183.091 kb on - strand, within EX31_RS00840 at 183.134 kb on - strand at 183.177 kb on + strand at 183.177 kb on + strand at 183.177 kb on + strand at 183.177 kb on + strand at 183.178 kb on - strand at 183.178 kb on - strand at 183.178 kb on - strand at 183.178 kb on - strand at 183.236 kb on - strand at 183.290 kb on + strand at 183.303 kb on + strand at 183.303 kb on + strand at 183.303 kb on + strand at 183.304 kb on - strand at 183.304 kb on - strand at 183.304 kb on - strand at 183.328 kb on + strand at 183.328 kb on + strand at 183.328 kb on + strand at 183.328 kb on + strand at 183.329 kb on - strand at 183.436 kb on + strand, within EX31_RS00845 at 183.437 kb on - strand, within EX31_RS00845 at 183.437 kb on - strand, within EX31_RS00845 at 183.437 kb on - strand, within EX31_RS00845 at 183.438 kb on - strand, within EX31_RS00845 at 183.453 kb on - strand, within EX31_RS00845 at 183.457 kb on - strand, within EX31_RS00845 at 183.457 kb on - strand, within EX31_RS00845 at 183.462 kb on - strand, within EX31_RS00845 at 183.462 kb on - strand, within EX31_RS00845 at 183.498 kb on - strand, within EX31_RS00845 at 183.509 kb on + strand, within EX31_RS00845 at 183.509 kb on + strand, within EX31_RS00845 at 183.510 kb on - strand, within EX31_RS00845 at 183.514 kb on + strand, within EX31_RS00845 at 183.546 kb on + strand, within EX31_RS00845 at 183.546 kb on + strand, within EX31_RS00845 at 183.581 kb on + strand, within EX31_RS00845 at 183.614 kb on - strand, within EX31_RS00845 at 183.617 kb on + strand, within EX31_RS00845 at 183.617 kb on + strand, within EX31_RS00845 at 183.617 kb on + strand, within EX31_RS00845 at 183.617 kb on + strand, within EX31_RS00845 at 183.617 kb on + strand, within EX31_RS00845 at 183.618 kb on - strand, within EX31_RS00845 at 183.618 kb on - strand, within EX31_RS00845 at 183.618 kb on - strand, within EX31_RS00845 at 183.693 kb on - strand, within EX31_RS00845 at 183.702 kb on - strand, within EX31_RS00845 at 183.707 kb on + strand, within EX31_RS00845 at 183.707 kb on + strand, within EX31_RS00845 at 183.708 kb on - strand, within EX31_RS00845 at 183.708 kb on - strand, within EX31_RS00845 at 183.752 kb on + strand, within EX31_RS00845 at 183.773 kb on + strand, within EX31_RS00845 at 183.849 kb on - strand, within EX31_RS00845 at 184.028 kb on + strand, within EX31_RS00845 at 184.029 kb on - strand, within EX31_RS00845 at 184.099 kb on + strand, within EX31_RS00845 at 184.104 kb on + strand, within EX31_RS00845 at 184.104 kb on + strand, within EX31_RS00845 at 184.104 kb on + strand, within EX31_RS00845 at 184.105 kb on - strand, within EX31_RS00845 at 184.105 kb on - strand, within EX31_RS00845 at 184.162 kb on + strand, within EX31_RS00845 at 184.163 kb on - strand, within EX31_RS00845 at 184.163 kb on - strand, within EX31_RS00845 at 184.327 kb on - strand at 184.327 kb on - strand at 184.455 kb on - strand at 184.557 kb on + strand at 184.557 kb on + strand at 184.829 kb on - strand at 184.829 kb on - strand at 184.832 kb on + strand at 184.993 kb on + strand, within EX31_RS00850 at 184.994 kb on - strand, within EX31_RS00850 at 185.167 kb on + strand, within EX31_RS00850 at 185.184 kb on + strand, within EX31_RS00850 at 185.186 kb on + strand, within EX31_RS00850 at 185.186 kb on + strand, within EX31_RS00850 at 185.186 kb on + strand, within EX31_RS00850 at 185.187 kb on - strand, within EX31_RS00850 at 185.345 kb on + strand, within EX31_RS00850 at 185.345 kb on + strand, within EX31_RS00850 at 185.352 kb on + strand, within EX31_RS00850 at 185.364 kb on + strand, within EX31_RS00850 at 185.365 kb on - strand, within EX31_RS00850
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 182,269 + -1.8 182,269 + +1.9 182,270 - +1.1 182,291 + +0.3 182,291 + +0.6 182,350 + +1.2 182,351 - +2.0 182,351 - +0.6 182,351 - -1.0 182,413 + EX31_RS00840 0.12 +0.8 182,414 - EX31_RS00840 0.12 -0.0 182,598 + EX31_RS00840 0.33 -0.4 182,598 + EX31_RS00840 0.33 -0.9 182,598 + EX31_RS00840 0.33 -0.0 182,649 + EX31_RS00840 0.38 +1.4 182,650 - EX31_RS00840 0.39 +0.4 182,849 + EX31_RS00840 0.61 -0.5 182,850 - EX31_RS00840 0.61 -0.0 182,906 - EX31_RS00840 0.67 +0.2 183,064 - EX31_RS00840 0.85 +1.8 183,083 + EX31_RS00840 0.87 +0.2 183,084 - EX31_RS00840 0.87 +0.7 183,091 - EX31_RS00840 0.88 -0.4 183,091 - EX31_RS00840 0.88 +0.4 183,134 - +0.7 183,177 + +0.5 183,177 + +1.0 183,177 + -0.2 183,177 + -2.2 183,178 - +1.0 183,178 - +0.1 183,178 - +2.0 183,178 - -0.3 183,236 - +1.0 183,290 + -0.1 183,303 + -0.5 183,303 + -0.0 183,303 + -1.0 183,304 - +0.4 183,304 - -0.4 183,304 - -0.0 183,328 + -0.7 183,328 + -1.3 183,328 + +0.7 183,328 + -0.4 183,329 - +0.7 183,436 + EX31_RS00845 0.17 +2.0 183,437 - EX31_RS00845 0.17 +0.5 183,437 - EX31_RS00845 0.17 +0.8 183,437 - EX31_RS00845 0.17 +2.7 183,438 - EX31_RS00845 0.17 -1.6 183,453 - EX31_RS00845 0.18 -0.0 183,457 - EX31_RS00845 0.19 +1.0 183,457 - EX31_RS00845 0.19 +1.4 183,462 - EX31_RS00845 0.19 -0.4 183,462 - EX31_RS00845 0.19 +0.2 183,498 - EX31_RS00845 0.22 +0.3 183,509 + EX31_RS00845 0.23 +0.5 183,509 + EX31_RS00845 0.23 +1.0 183,510 - EX31_RS00845 0.23 -0.2 183,514 + EX31_RS00845 0.24 -1.0 183,546 + EX31_RS00845 0.27 +0.6 183,546 + EX31_RS00845 0.27 +0.6 183,581 + EX31_RS00845 0.30 +1.4 183,614 - EX31_RS00845 0.33 +0.3 183,617 + EX31_RS00845 0.33 +0.6 183,617 + EX31_RS00845 0.33 -1.2 183,617 + EX31_RS00845 0.33 -0.0 183,617 + EX31_RS00845 0.33 +0.7 183,617 + EX31_RS00845 0.33 +2.0 183,618 - EX31_RS00845 0.33 +0.5 183,618 - EX31_RS00845 0.33 +0.3 183,618 - EX31_RS00845 0.33 +1.0 183,693 - EX31_RS00845 0.40 -0.0 183,702 - EX31_RS00845 0.40 +1.0 183,707 + EX31_RS00845 0.41 +0.0 183,707 + EX31_RS00845 0.41 +0.6 183,708 - EX31_RS00845 0.41 -1.4 183,708 - EX31_RS00845 0.41 +0.1 183,752 + EX31_RS00845 0.45 +0.2 183,773 + EX31_RS00845 0.47 +0.7 183,849 - EX31_RS00845 0.53 -0.9 184,028 + EX31_RS00845 0.69 -1.4 184,029 - EX31_RS00845 0.69 +1.1 184,099 + EX31_RS00845 0.76 -2.4 184,104 + EX31_RS00845 0.76 -0.4 184,104 + EX31_RS00845 0.76 +1.0 184,104 + EX31_RS00845 0.76 +0.2 184,105 - EX31_RS00845 0.76 +1.6 184,105 - EX31_RS00845 0.76 +3.5 184,162 + EX31_RS00845 0.81 +1.3 184,163 - EX31_RS00845 0.81 -0.4 184,163 - EX31_RS00845 0.81 -0.2 184,327 - +0.2 184,327 - -2.5 184,455 - +1.2 184,557 + -0.3 184,557 + +0.2 184,829 - +1.5 184,829 - -1.0 184,832 + +1.4 184,993 + EX31_RS00850 0.12 -0.0 184,994 - EX31_RS00850 0.12 -0.6 185,167 + EX31_RS00850 0.37 +0.4 185,184 + EX31_RS00850 0.39 +0.7 185,186 + EX31_RS00850 0.39 +0.7 185,186 + EX31_RS00850 0.39 -0.5 185,186 + EX31_RS00850 0.39 +2.5 185,187 - EX31_RS00850 0.40 +3.3 185,345 + EX31_RS00850 0.62 -1.6 185,345 + EX31_RS00850 0.62 -0.5 185,352 + EX31_RS00850 0.63 +0.4 185,364 + EX31_RS00850 0.65 +1.3 185,365 - EX31_RS00850 0.65 +0.0
Or see this region's nucleotide sequence